GenBank-Updates@genbank.bio.net (04/19/91)
LOCUS PTITETLMOB 5413 bp ds-DNA BCT 19-APR-1991
DEFINITION Plasmid pTBig (from Bacillus sp.) replication protein (rep),
tetracycline-resistance protein (tetL) and mobilizable protein
(mob) genes, complete cds
ACCESSION M63891
KEYWORDS mob gene; mobilizable protein; rep gene; replication protein;
tetL gene; tetracycline resistance protein.
SOURCE Plasmid pTBig (from Bacillus sp.) DNA.
ORGANISM Plasmid pTBig
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 5413)
AUTHORS Oskam,L., Hillenga,D.J., Venema,G. and Bron,S.
TITLE The large bacillus plasmid pTBig contains two integrated rolling
circle plasmids
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS join(5098..5347,1219..1481)
/product="replication protein"
/gene="rep"
/codon_start=5098
CDS complement(1..1216)
/note="ORF"
/codon_start=1216
/partial
CDS 1681..3057
/product="tetracycline resistance protein"
/gene="tetL"
/codon_start=1681
misc_feature 3177..3448
/note="Palu minus origin of replication"
misc_feature 3527..3551
/note="RSa site"
CDS 3622..4869
/product="mobilizable protein"
/gene="mob"
/codon_start=3622
rep_origin 4930..4946
BASE COUNT 1717 a 822 c 1094 g 1780 t
ORIGIN
1 aagcttcttt caattgactt tctttaattc gttttccatc ttttgttttg ttcaaagttt
61 cttctcggta ttcttcattt tgatcaataa caacgcctga aaaagctacc agcacatcta
121 tatcatcgta gcctttttcc ttaatatact tcttaaactc atggaaataa cggactgcat
181 gtaaacgaga agctgtgaca accattgctt ttgctttacc gccaatttta tgtctagtta
241 cattccgaaa ttgttcgacc atgatagccg tcttttgttg tagagtatat ggatgtaatg
301 attgataacg cttgatagct tttactccct gtgttgtcga taattccgga ttctctgatg
361 tgtttttggc aatacgataa tatgttttgt aggtcatata attttgtaat acatctaata
421 taaatccttc ttcgatagct tgacgcatac tgtaaatatg aaaaggttta aacgttccat
481 ctggttgttt tgtcccaaac atttctaatg tcttttcttt aggagtagct gtaaaagcaa
541 agaaacttaa atttttatgt ctgccatgtg tcagaagttc ctgaacgatt tcatcttcaa
601 aatctaactg ctcagcttcg atttctgctt ccagttctgc atattcccgt aacgcttcct
661 ctgtatctgc taatgctgct tttaattttt tcgcactttt tcctgtctgg gaggaatgtg
721 cttcatctac aataacggca aagcgacttc ccttgttaac ttctacttct tcataaataa
781 caggaaactt ttgaagtgtc gtgataataa ttcgttttcc gttattaata gcatctctta
841 aatctttgga agtctttttg tcatcaatgg tttctacaag ccctgttgta tgatcgaaac
901 tggaaattgt ttgttgtaat tgccgatcaa gtactgttcg atctgttacc acgataactg
961 atgtaaaaat gctttcatta tcttcattgt gcaaacttgc taaatgatat gccaaccatg
1021 ctatgctgtt cgactttcca gaacctgcgc tgtgttggat aagataattt tccccgcttc
1081 ctttttgacg aacatcttca acaagttttc tgaccacatc taactgatga tagcgtggga
1141 aaatcagctt agaacttgtt ttgacgcttt cctttccgtt tttgataaac ttttcttttt
1201 ttacttctaa atgcatgaat cctgtaaagg atacggattt tatgaccgat gatgaagaaa
1261 agaatttgaa acgtttgtct gatttggagg aaggtttaca ccgtaaaagg ttaatctcct
1321 atggtggttt gttaaaagaa atacataaaa aattaaacct tgatgacaca gaagaaggcg
1381 atttgattca tacagatgat gacgaaaaag ccgatgaaga tggattttct attattgcaa
1441 tgtggaattg ggaacggaaa aattatttta ttaaagagta gttcaacaaa cgggccatat
1501 tgttgtataa gtgatgaaat actgaattta aaacttagtt tatatgtggt aaaatgtttt
1561 aatcaagttt aggaggaatt aattatgaag tgtaatgaat gtaacagggt tcaattaaaa
1621 gagggaagcg tatcattaac cctataaact acgtctgccc tcattattgg agggtgaaat
1681 gtgaatacat cctattcaca atcgaattta cgacacaacc aaattttaat ttggctttgc
1741 attttatctt tttttagcgt attaaatgaa atggttttga acgtctcatt acctgatatt
1801 gcaaatgatt ttaataaacc acctgcgagt acaaactggg tgaacacagc ctttatgtta
1861 accttttcca ttggaacagc tgtatatgga aagctatctg atcaattagg catcaaaagg
1921 ttactcctat ttggaattat aataaattgt ttcgggtcgg taattgggtt tgttggccat
1981 tctttctttt ccttacttat tatggctcgt tttattcaag gggctggtgc agctgcattt
2041 ccagcactcg taatggttgt agttgcgcgc tatattccaa aggaaaatag gggtaaagca
2101 tttggtctta ttggatcgat agtagccatg ggagaaggag tcggtccagc gattggtgga
2161 atgatagccc attatattca ttggtcctat cttctactca ttcctatgat aacaattatc
2221 actgttccgt ttcttatgaa attattaaag aaagaagtaa ggataaaagg tcattttgat
2281 atcaaaggaa ttatactaat gtctgtaggc attgtatttt ttatgttgtt tacaacatca
2341 tatagcattt cttttcttat cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc
2401 aggaaagtaa cagatccttt tgttgatccc ggattaggga aaaatatacc ttttatgatt
2461 ggagttcttt gtgggggaat tatatttgga acagtagcag ggtttgtctc tatggttcct
2521 tatatgatga aagatgttca ccagctaagt actgccgaaa tcggaagtgt aattattttc
2581 cctggaacaa tgagtgtcat tattttcggc tacattggtg ggatacttgt tgatagaaga
2641 ggtcctttat acgtgttaaa catcggagtt acatttcttt ctgttagctt tttaactgct
2701 tcctttcttt tagaaacaac atcatggttc atgacaatta taatcgtatt tgttttaggt
2761 gggctttcgt tcaccaaaac agttatatca acaattgttt caagtagctt gaaacagcag
2821 gaagctggtg ctggaatgag tttgcttaac tttaccagct ttttatcaga gggaacaggt
2881 attgcaattg taggtggttt attatccata cccttacttg atcaaaggtt gttacctatg
2941 gaagttgatc agtcaactta tctgtatagt aatttgttat tacttttttc aggaatcatt
3001 gtcattagtt ggctggttac cttgaatgta tataaacatt ctcaaaggga tttctaaatc
3061 gttaagggat caactttggg agagagttca aaattgatcc tttttttata acaggaattc
3121 aaatcttttt gttccattaa agggcgcgat tgctgaataa aagatacgag agacctctct
3181 tgtatctttt ttattttgag tggttttgtc cgttacacta gaaaaccgaa agacaataaa
3241 aattttattc ttgctgagtc tggctttcgg taagctagac aaaacggaca aaataaaaat
3301 tggcaagggt ttaaaggtgg agattttttg agtgatcttc tcaaaaaata ctacctgtcc
3361 cttgctgatt tttaaacgag cacgagagca aaacccccct ttgctgaggt ggcagagggc
3421 aggttttttt gtttcttttt tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt
3481 tggtcggcac tgccgacagc ctcgcagagc acacacttta tgaatataaa gtatagtgtg
3541 tttatacttt acttggaagt ggttgccgga aagagcgaaa atgcctcaca tttgtgccac
3601 ctaaaaagga gcgatttaca tatgagttat gcagtttgta gaatgcaaaa agtgaaatca
3661 gctggactaa aaggcatgca atttcataat caaagagagc gaaaaagtag aacgaatgat
3721 gatattgacc atgagcgaac acgtgaaaat tatgatttga aaaatgataa aaatattgat
3781 tacaacgaac gtgtcaaaga aattattgaa tcacaaaaaa caggtacaag aaaaacgagg
3841 aaagatgctg ttcttgtaaa tgagttgcta gtaacatctg accgagattt ttttgagcaa
3901 ctggatccag gagaacaaaa acgatttttt gaggaaagtt ataaattatt ttccgaacga
3961 tatggcaagc aaaatattgc ttatgcaaca gttcataatg atgagcaaac ccctcacatg
4021 catttaggtg ttgtgcctat gcgtgatgga aaactgcaag gaaaaaatgt gtttaatcgt
4081 caagaactgt tatggctaca agataaattc cccgagcata tgaaaaaaca gggttttgag
4141 ttgaagcgtg gtgaacgtgg ctctgaccgt aaacatattg agacagctaa atttaaaaaa
4201 caaactttgg aaaaagagat tgatctttta gagagcgaat tgaaaaataa aaaaagtgag
4261 ttggcgattt tatctgaaga agtttcgggt gaatttaaga ttccagtgaa aagagaaaag
4321 aagagcgttg aagttccgac agggaaacga aatcttttgg ggatcgagca aaagaaaacg
4381 gttatgaaat caacggggaa tgtaatttta aaagacgaag tgtttcaaga tttgaagcaa
4441 aaagtaaaag caggcgcagt tttgcaaacg agagtggatc agttgctgaa tactgattac
4501 gccaaagaga atcaatcgtt gaaaaaagaa gtgaaagagt tgcgatctac aaataaatct
4561 ttatccgaag aaaacggtcg tttaaagagt acagtggagc atctaacgaa tgagatcgaa
4621 agtttatatg tattgacgaa agacttttta aaagcccgta caaacgattt agagagtttt
4681 aaggagttgt tcggggtatt tgtaggtaaa gtgaaagaaa aagcgcctag agggcttttc
4741 gtgcgtcagc atgagcgatc agaagaaaag aagaatactt tttcgcttca agatgttttg
4801 cagcgtgatc gagaacttcg tgagcaaaga aaagcaaaga ggaaaaaatc gcatgatttg
4861 gaacgataag aaaaggcact cgaatgagtg ccttttgtgc gttttgagcg tagcgaaaaa
4921 acggttcttt cttatcttga tacatataga aataacgtca tttttatttt agttgctgaa
4981 aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat tgaaaaccct taaaattggt
5041 tgcacagaaa aaccccatct gttaaagtta taagtgacca aacaaataac taaatagatg
5101 ggggtttctt ttaatattat gtgtcctaat agtagcattt attcagatga aaaatcaagg
5161 gttttagtgg acaagacaaa aagtggaaaa gtgagaccat ggagagaaaa gaaaatcgct
5221 aatgttgatt actttgaact tctgcatatt cttgaattta aaaaggctga aagagtaaaa
5281 gattgtgctg aaatattaga gtataaacaa aatcgtgaaa caggcgaaag aaagttgtat
5341 cgagtgtttg agtcaagtat ttataggcag tttttttaac accttaaatt tgaaagcgga
5401 ttcaaacctt agg
//