GenBank-Updates@genbank.bio.net (04/23/91)
LOCUS ECOICLRA 1223 bp ds-DNA BCT 23-APR-1991
DEFINITION E.coli repressor protein for the aceBAK operon (iclR) gene,
complete cds.
ACCESSION M63914 M34937
KEYWORDS aspartokinase II-homoserine dehydrogenase II; iclR gene; metL gene;
regulatory protein of the aceBAK operon; repressor.
SOURCE E.coli (strain K-12) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1223)
AUTHORS Negre,D., Cortay,J.-C., Old,I.G., Galinier,A., Richaud,C., Saint
Girons,I. and Cozzone,A.J.
TITLE Overproduction and characterization of the iclR gene product of
Escherichia coli K-12 and comparison with that of Salmonella
typhimurium LT2
JOURNAL Gene 97, 29-37 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS complement(1..20)
/product="aspartokinase II-homoserine dehydrogenase II"
/gene="metL"
/codon_start=20
/partial
-10_signal complement(70..75)
/gene="metL"
-35_signal complement(93..98)
/gene="metL"
protein_bind complement(116..127)
/note="MetR binding site"
-35_signal 147..152
/gene="iclR"
-10_signal 170..175
/gene="iclR"
CDS 181..1044
/product="repressor of the aceBAK operon"
/gene="iclR"
/codon_start=181
BASE COUNT 333 a 309 c 288 g 293 t
ORIGIN
1 tgttccactt tgctgctcac acttgctccc gacacgctca acccagattt aataaaaatt
61 caacaaacca tactggcata aacgcatctg tggtaaaagc gaccaccacg caacatgaga
121 tttgttcaac attaactcat cggatcagtt cagtaactat tgcattagct aacaataaaa
181 atgaaaatga tttccacgat acagaaaaaa gagactgtca tggtcgcacc cattcccgcg
241 aaacgcggca gaaaacccgc cgttgccacc gcaccagcga ctggacaggt tcagtcttta
301 acgcgtggcc tgaaattact ggagtggatt gccgaatcca atggcagtgt ggcactcacg
361 gaactggcgc aacaagccgg gttacccaat tccacgaccc accgcctgct aaccacgatg
421 caacagcagg gtttcgtgcg tcaggttggc gaactgggac attgggcaat cggcgcacat
481 gcctttatgg tcggcagcag ctttctccag agccgtaatt tgttagcgat tgttcaccct
541 atcctgcgca atctaatgga agagtctggc gaaacggtca atatggcggt gcttgatcaa
601 agcgatcacg aagcgattat tatcgaccag gtacagtgta cgcatctgat gcgaatgtcc
661 gcgcctatcg gcggtaaatt gccgatgcac gcttccggtg cgggtaaagc ctttttagcc
721 caactgagcg aagaacaggt gacgaagctg ctgcaccgca aagggttaca tgcctatacc
781 cacgcaacgc tggtgtctcc tgtgcattta aaagaagatc tcgcccaaac gcgcaaacgg
841 ggttattcat ttgacgatga ggaacatgca ctggggctac gttgccttgc agcgtgtatt
901 ttcgatgagc accgtgaacc gtttgccgca atttctattt ccggaccgat ttcacgtatt
961 accgatgacc gcgtgaccga gtttggcgcg atggtgatta aagcggcgaa ggaagtgacg
1021 ctggcgtacg gtggaatgcg ctgacttttt ctggcgggca gaggcaatat tctgcccatc
1081 atacctgagt ggcaatagaa taagggtgtc tgttaatcgc attgacgcca aaataactta
1141 atgtcataca cttcacacac ttttattaac tcagcattat ttttaaacat caaaccactt
1201 aattattaac aatcgcaaga tct
//