GenBank-Updates@genbank.bio.net (04/23/91)
LOCUS MUSSPI2A 1781 bp ss-mRNA ROD 23-APR-1991
DEFINITION Mouse spi2 proteinase inhibitor (spi2/eb1) mRNA, 3' end.
ACCESSION M64085
KEYWORDS proteinase inhibitor; serpin; serum protein.
SOURCE Mouse (strain 129) embryo chondrocytic EB22 teratocarcinoma cell
line, cDNA to mRNA, clone pEB22/1.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1781)
AUTHORS Inglis,J.D., Lee,M., Davidson,D. and Hill,R.E.
TITLE Isolation of two novel murine serpin-encoding cDNAs from a
chondrocyte cell-line
JOURNAL Unpublished (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 3..1164
/product="spi2 proteinase inhibitor"
/gene="spi2/eb1"
/codon_start=4
/partial
misc_feature 952..997
/note="reactive centre"
/gene="spi2/eb1"
BASE COUNT 448 a 478 c 422 g 433 t
ORIGIN chromosome 12
1 tgaccaccag gagccagata tcacagacac tggcagctgg ctcagctctg cagcatctgc
61 actgccttgg ccctcctgtc cctgggagca aagagcaaca ccctgaagga aatcctagaa
121 ggtctcaagt tcaacctcac agagacccct gaaccagaca tccaccaggg ctttaggtac
181 ttgctagacc ttctaagtca gccagggaac caggtacaga tcagcacagg cagtgccctg
241 tttattgaaa agcacctaca gatcctggca gagttcaagg agaaggcaag ggctctgtac
301 caggctgagg ccttcacagc agatttccag caacctctca aggccacaaa gctcatcaat
361 gactatgtgg gcaatcacac ccaggggaac atcaaggaac tcatctcagg cctgaaagag
421 agcacattga tggtgctggt gaactacatc tactttaaag gcaaatggaa gaaccccttt
481 gacccgaatg atacatttaa gtccgagttc tacttggatg agaagaggtc tgtgattgtg
541 tccatgatga aaactggtta cctgacaact ccctacttcc gggatgagga gctgtcctgc
601 actgtggtgg agctgaaata cacaggaaat gccagtgcca tgttcatcct ccctgaccag
661 actgtggtgg agctgaaata cacaggaaat gccagtgcca tgttcatcct ccctgaccag
721 tctctgaagc ccaggatgat acatgagctc cgcctgccca agttctccat ctccaccgac
781 tacagcctgg agcacatcct tcctgagttg ggcatcaggg aagtcttctc cacacaggct
841 gacctgtctg caatcacagg aaccaaggat ctgagagtct ctctggtggt ccacaaggct
901 gtgctggatg tggctgagac aggcacagag gctgctgctg ccacaggaat ggcaggtgtc
961 ggatgttgtg cagtttttga ctttctggaa atatttttca acaggccatt cctgatgatt
1021 atctctgaca caaaagctca cattgccctc tttatggcaa aagttacaaa tccagagaga
1081 tgtatgaact tcccaaatgg tgagggtgct tcttcccaac ggctggaatc taagcgtttg
1141 tttttggtga tccgctatgc ctgattggac agtgacactt accaactctg tcacgtcctc
1201 atgcagagaa agaagcctat ggctggttat tgtcagactc tctgtcatga tggtatcacg
1261 aaatcaggtt cctgacctga aatttgtgtt attccctttc cctgctgtct ccattgtatc
1321 tgcctcaacc caaaagcctg ggcccagcag tgaggctctc tcctaactta ggctctgctt
1381 atgtctgcct tcatcctttc agtcatgatg ggactgtgca agtttacagg ccaacccata
1441 aggactaaga aaggaacctg gtgccctggg tctatctgca gcatctgaaa tgcttggtgc
1501 ccagatctgc cttatgcttg ccttcctctg ggagaattat cctcagcccc tgcatagtct
1561 cctggcccca cccagatctg gcacaggtgg agatctcagc cctgcagctg catggggcct
1621 gtgggtcaga gcagctcctc cccctgtagc actcctactc agaacagagt agtctttctc
1681 aatgtctctt gttgaccaac tacgtaggac tggtggacac ctctgcatta ctcacacaaa
1741 ttgaaagggc aggagctaag gatcaataaa catgtaactg c
//GenBank-Updates@genbank.bio.net (04/23/91)
LOCUS MUSSPI2B 2035 bp ss-mRNA ROD 23-APR-1991
DEFINITION Mouse spi2 proteinase inhibitor (spi2/eb4) mRNA, complete cds.
ACCESSION M64086
KEYWORDS proteinase inhibitor; serpin; serum protein.
SOURCE Mouse (strain 129) embryo chondrocytic EB22 teratocarcinoma cell
line, cDNA to mRNA, clone pEB22/4.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 2035)
AUTHORS Inglis,J.D., Lee,M., Davidson,D. and Hill,R.E.
TITLE Isolation of two novel murine serpin-encoding cDNAs from a
chondrocyte cell-line
JOURNAL Unpublished (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 61..1317
/product="spi2 proteinase inhibitor"
/gene="spi2/eb4"
/codon_start=61
misc_feature 1189..1234
/note="reactive centre"
/gene="spi2/eb4"
BASE COUNT 505 a 564 c 492 g 474 t
ORIGIN chromosome 12
1 cacgagcaca gaggctggca gctggctggt ttcagctctg cagactgcag aacacagaag
61 atggctttca ttgcagctct ggggctcttg atggctggga tctgccctgc tgtcctctgc
121 ttcccagatg gcacgttggg aatggatgct gcagtccaag aagaccatga caatgggaca
181 caactggaca gtctcacatt ggcctccatc aacactgact ttgccttcag cctctacaag
241 gagctggttt tgaagaatcc agataaaaat attgtcttct ccccacttag catctcagcg
301 gccttggctg tcatgtccct gggagcaaag ggcaacaccc tggaagagat tctagaaggt
361 ctcaagttca atcttacaga gacctctgag gcagacatcc accagggctt tgggcacctc
421 ctacagaggc tcaaccagcc aaaggaccag gtacagatca gcacgggtag tgccctgttt
481 attgaaaagc gccagcagat cctgacagaa ttccaggaga aggcaaagac tctgtaccag
541 gctgaggcct tcacagcaga cttccagcag cctcgacagg ccaaaaagct catcaatgac
601 tatgtgagga aacagaccca ggggatgatc aaggaactgg tctcagacct ggataaaagg
661 acattgatgg tgctggtgaa ttatatctac tttaaagcca aatggaaggt gccctttgac
721 cctcttgaca cgttcaagtc tgagttctac tgcggcaaga ggaggcccgt gatagtgccc
781 atgatgagca tggaggacct gaccacaccc tacttccgag atgaggagct gtcctgcact
841 gtggtggagc tgaagtacac aggaaatgcc agcgccctgt tcatcctccc tgaccagggc
901 aggatgcagc aggtggaagc cagcttacaa ccagagaccc tgaggaagtg gaagaattct
961 ctgaaaccca ggatgataga tgagctccac ctgcccaagt tctccatctc caccgactac
1021 agcctggagg atgtcctttc aaagctgggc atcagggaag tcttctccac acaggctgac
1081 ctgtctgcaa tcacaggaac caaggatctg agagtgtctc aggtggtcca caaggctgtg
1141 ctggatgtgg ctgagacagg cacagaagca gctgctgcca ctggagtcaa atttgtccca
1201 atgtctgcga aactgtaccc tctgactgta tatttcaatc ggcctttcct gataatgatc
1261 tttgacacag aaactgaaat tgcccccttt atagccaaga tagccaaccc caaatgagac
1321 tagaactccc caagtgttga cgcttcttcc cgggagccag gcattgagcc tgtctgtggg
1381 tctccatgtg cattttggct tccatgctct gcttggcctt ggcatgcctg gattagatag
1441 tgactaactg tgttataacc tcatgtacag acatccctgt gggaagtcag tgccgtgctc
1501 ccagacttct tggtagcact agcccatgtt cctgagcctg aaatttgtct tgtcccctac
1561 ccctgctctc tccctgtatc tgcctccacc caaaagcctg ggccccatca agtaagctca
1621 gtcccagttt agactctggt tatgtctccc ttcagccttg ctgtcttgat gggactgtgc
1681 aaccttacag gccaacccat atggaccaag aggaaagctt ggctggcccg tgtctatccg
1741 cagcatctag gactacttgg tgcccagtct gcctcactct ttcatttctc caggctttgc
1801 ctccaggcac tgcccccctg caggatctcc tcactttgtc cacatctggc acagagttag
1861 agccctcact tctgcagctg catggggtct gtgggtcaga gcagatcctt tcccctggca
1921 ctcctactta gaacaaagta gcctttcttt tagttcccag ctgaccaacc tcacacaaaa
1981 gaggaacacc aaccagaata aagaggtagg agcaaaggat caataaacat gtaac
//