GenBank-Updates@genbank.bio.net (04/23/91)
LOCUS LBAEMRR 938 bp ds-DNA BCT 23-APR-1991
DEFINITION L.reuteri methylase (emrR) gene, complete cds.
ACCESSION M64090
KEYWORDS MLS antibiotics resistance; erythromycin resistance; methylase.
SOURCE L.reuteri (strain 100-63) DNA.
ORGANISM Lactobacillus reuteri
Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods;
Lactobacillaceae.
REFERENCE 1 (bases 1 to 938)
AUTHORS Connell,H., Tannock,G.W., Luchansky,J.B., Mercer,A.A., Miller,L.A.
and Klaenhammer,T.R.
TITLE Characterization of a plasmid (pGT633) from Lactobacillus reuteri
that encodes resistance to MLS antibiotics
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS join(23..79,168..902)
/function="erythromycin resistance"
/product="methylase"
/gene="emrR"
/evidence=EXPERIMENTAL
/codon_start=23
sig_peptide 23..79
/pseudo=true
/function="inoperative translational attenuator"
/note="leader peptide"
/gene="emrR"
/evidence=EXPERIMENTAL
/codon_start=23
stem_loop 111..149
/function="2:3 stem loop prediction for `on' conformation"
/gene="emrR"
stem_loop 138..172
/function="3:4 hairpin prediction for `off' conformation"
/gene="emrR"
mat_peptide 168..899
/function="erythromycin resistance"
/product="methylase"
/gene="emrR"
/evidence=EXPERIMENTAL
/codon_start=168
stem_loop 898..938
/function="terminator"
/gene="emrR"
BASE COUNT 395 a 119 c 125 g 299 t
ORIGIN
1 cataaaaaag gagaattaat atatgggcat ttttagtatt tttgtaatca acacagttca
61 ttatcaacca aacaaaaaat aagtggttat caaccaaaca aaaaataagt ggttataatg
121 gattgttaat ataaacattc attataacct cataggagtg ggttataatg aacaaaaaaa
181 atataaaaga tagtcaaaac tttattactt caaagcatca tataaatgaa attttgagaa
241 atgtacattt aaatacaaat gataatatta ttgagattgg ttcagggaaa ggtcatttct
301 cgtttgaatt agctaaaagg tgtaattatg taaccgccat tgaaatagat cctaaattat
361 gtaggataac taaaaacaaa cttattgaat atgagaactt ccaggttatc aataaagata
421 ttttacaatt taagtttcct aaaaataagt catataagat atttggaaat ataccctaca
481 atataagtac agatataatt cgaaaaattg tttttgaaag cacagctaca gaaagttatt
541 taatagtgga atatggattt gctaaaaggt tgctaaatac aaatcgttca ctagcactat
601 ttttaatgac agaagttgat atatccatat taagtaaaat ccctagagaa tactttcatc
661 caaaacctag agttaatagc tcgttaattg tattaaaaag acacccttca aaaatatcac
721 tcaaagatag aaaacaatat gaaaattttg ttatgaaatg ggttaacaaa gaatacataa
781 aactattttc caaaaaccaa ttttatcaag ccttaaaata tgcaagaatt gacgatttaa
841 acaatattag ctttgaacaa ttcttgtctc ttttcaatag ctataaatta tttaatagat
901 aagttaaggg gtgcataaac tgcatccctt aacttatc
//