GenBank-Updates@genbank.bio.net (04/23/91)
LOCUS VIBTDHAB 6816 bp ds-DNA BCT 23-APR-1991
DEFINITION V.mimicus thermostable direct hemolysin gene, complete cds.
ACCESSION M64120
KEYWORDS thermostable direct hemolysin.
SOURCE V.mimicus DNA.
ORGANISM Vibrio mimicus
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (sites)
AUTHORS Terai,A., Baba,K., Shirai,H., Yoshida,O., Takeda,Y. and
Nishibuchi,M.
TITLE A molecular basis for insertion sequence-mediated spread of the
thermostable direct hemolysin gene among Vibrio species
JOURNAL Unpublished (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
-35_signal 1440..1445
/gene="tdh"
-10_signal 1462..1467
/gene="tdh"
RBS 1504..1508
/gene="tdh"
CDS 1515..2084
/product="thermostable direct hemolysin"
/gene="tdh"
/codon_start=1515
terminator 2091..2116
/gene="tdh"
BASE COUNT 2234 a 1136 c 1359 g 2087 t
ORIGIN
1 gagctcatca gagattatga ttgaaattcg ttttcttaaa tcatcatttt ttctatactt
61 gaaatttgtt cttgacaaac aatattaatc aactcttttg ctgcagcgat tgtcggtatc
121 tttaaatttt tctctctgat cttttcatcg attcttctat caagcaactc ataagctttt
181 tgtagcacat ctgctcgttc atcttttgag agttccttaa attcttgatt tcgttccaat
241 agagatgtta agtagttttg taacggacct gagcacatta aatactccat ctttatacca
301 gcgagaatct tttacttaat agtatcccgt ggctttgttg cgtaaatcga tggaactaaa
361 ttcaaagctt acgatccgat cggctacgtt cattcaactc tgtagtctca tgcctaaacc
421 acgttacaaa acaaacaact ggaagcaata caatcaagct cccatcaacc gcggctctat
481 gacgttttgg attgatgaag aagcgataag agagtggaaa caaggcaagg aaaacaagcg
541 cgggaaacct cgtattttca gtgatttggc catcacaacc gctttgattg taaagcgcat
601 tttttcaatg ccacttcgcg cttcgcaagg gtttatggat cccgtgtttc gacttgctca
661 acttccactg cgctgcccac actacagttg tatcagttgt cgagctaaag acgttgatgt
721 aagttttaaa aagaaaacca aaggaccgat tcacatcttg ctatcgatgc gacaggtctc
781 aaggtttatg gtgagggcga gtggaaagtc aaaaaacatg gcacggatgg taaacgcaga
841 gtgtggcgga aattacatct cgtagtagat acgagtacgc acgaaattat tgccgcagag
901 ttgagtttat caacggtgag tgaggcagaa gtgctgccta atttgcttaa gcaaacacgt
961 cgaaaaatct ttgagatatc aggcgatggg gcttatgaca cgagggattg ccacgcagca
1021 atccgtatta gacgaaccgt ggctcttatc cctccaagag agggagcggc attctgggag
1081 agaggtcatc tgagaaatgg ttgccaaaag ctatatggtt cgaacaagca ttggaaaaag
1141 cactatggct accacaagcg ctcgctatct gaaacggcga tgtatcgagt caaacagttg
1201 cttggtggac ggctaagtct aagaaattac aacgcacaag ttggtgaaac ttacgcgatg
1261 atcaaagcgt tgaacaagct tacaggcttg gtatgcctat tggttgacaa ttaatctaaa
1321 tagggctgtt cggcttcgaa aatgacttac gcaacaaagc cattcaggca ttacggttac
1381 ttatagagtt gtaagcacta tcaaagattt gggaggtgtt tttttcatga ttattcagtt
1441 tgctttttgg ttttttttag gtttcatgac gtctgccatt ctggcaaagt tattaatcaa
1501 ctcataggtt ttttatgaaa taccaatatt ttgcaaaaaa atcattttta tttatatcca
1561 tgttggctgc attcaaaaca tttgcttttg agcttccatc tgtccctttt cctgcccccg
1621 gttctgatga gatattgttt gttgttcgag atacaacttt taataccaaa gctccggtca
1681 atgtaaaggt ctctgacttt tggacaaacc gtgatgtaaa aagaaaaccg tacgaagatg
1741 tttatggtca atcagtattc acaacgtcag gtactaaatg gttgacatcc tacatgactg
1801 tgaacattaa tgataaagac tatacaatgg cagcggtgtc tggctataag agcggtcatt
1861 ctgctgtgtt cgtaaaatca gatcaagtac agcttcaaca ttcctataat tctgtagcca
1921 actttgttgg tgaagatgaa gattctattc caagtaaaat gtatttggat gaaactccag
1981 aatattttgt taatgtagaa gcatatgaaa gtggtagtgg taatgtattg gtaatgtgta
2041 tatccaacaa agaatcgttt tttgaatgtg aacatcaacg ataaaagtaa aaagccacag
2101 aatattctgt ggctttgaaa ataattaata ataattttct gatttatgca tgtcaatgca
2161 aagctaggtt gagcacatgt catgtttttt attatagcca acaaccaaag tctcgcttgt
2221 tgataaatat cagacaaaag agaatgatta ttaataatgt tttagcttat ggttttttat
2281 gaaatttaaa gtgaatgttt ttgcaattgt gctatgtttg ttttcacgct atttattcgc
2341 ttccgatggt tacactttag attcaaaagt atcctccgtt agctttgcta ctattaaaag
2401 tcagtatgtg gttgagccgg cggtttttgg gaacttatct gggtcaatag atgagaatgg
2461 aagttttgaa atttctattg atattaatag tataaataca ggtatatcta ttcgagatac
2521 tcgtttgagt gatatattat ttgagtcttt taaatatcct aaagttaatg ttgttggcaa
2581 ggtggattta aatatattaa acgagggtgt ccaaaatata acagtatcat ctgatgtaaa
2641 attttttgat aaaataaaaa caatcaactt tccagtgaca gtattaaaat ctaataacta
2701 tttgatggtt agttcaagtt cccctgtcgt cataagcatg tctgactttg atattcctag
2761 tagtaactta atagagttgg ccaaaaccgt tggtggtatt agcatttcta gccaagtgcc
2821 actcatgttg tgcttgtcgt ttaaaaaata ataactatca ccaaaagttg tagaaagcag
2881 aacgatgagt tttcgattgt attatttaac tttttgagaa aatcaagtat gattagtgac
2941 aatatatgaa ttaaagaaca ggagaatatt tatatgtaaa agaaagatct tattaaaaat
3001 gcatattagc ctgattgtgt tattggtttt agttatcgtg atagaagtta ggtaggataa
3061 ttaaatattt cattttgtta aagattaaat ttatcattag cgacaaaact ttgagaaaat
3121 aaacatgaat aaattattat ttttacttat cgtgctatta tcaccaatag catcttccca
3181 agaatcaacg attattaaca acatggactt tggcccttta gctaagttga taggtgaatg
3241 gaaaacggct gatactggag ggattgatgt tgcccctgct caagaaggtt caacatcagg
3301 aaaaggagga cctgcagttg aatcatttta tgaaacaata acttttgaac cggctgctga
3361 tgtcaaaaat gcaagtgatc aatatcaagt gatcaatatc tagtagctct ttactacaaa
3421 caagaagtat ttagaaagcg agataatggt aaatttcatg atcagcgagg ttatttgatt
3481 tatgataaga aaaaccaaaa aatttataac tcttactgta ttccaagggc tgtttgtatt
3541 gtggcagaag gtcaggttgg cgataaaata caattcaaaa caatgcaaaa tggagtagca
3601 gaaagtgaat acctgcttga aaatgacaca acgcaaggtt ttataatgac attagatata
3661 agtgaggatg atgtaattag ttatactcaa acgacacagc taagagttta tggtaaaccg
3721 ttttcccata cagatactag cactttagtt cgagtgaatt aatgtattta taagctttaa
3781 aatgttttaa tgtgattaat ttttaataat taataaaaac cagtaaaaac actggttttg
3841 tatagaagca agctctaccg ctttaatgcg gtcacgcact cgactatctc gcatttgagt
3901 gtgctggttt tcgtgtttat gtttttgatt tttggtcagg gttactttta aggggcgtag
3961 cctgaacttt atgccaataa aaatcaagca tattcaataa tcacgagaat gatagcaaac
4021 cgtgtttacc gatatgataa atgttgaaaa taataaatca tttttcactg gtgtcttggc
4081 tatataacat agttttattg aagaaaaccc aataaatata aggaatggct atgataaaaa
4141 tacgaaactc atcatttcat attcagcaaa gttcgcattc aacgactaag aatcggtttc
4201 agtcattgat ggtaaataag atccagcata ccccatcaaa agaagctatt tgttacaagc
4261 cttccgctaa taaaagagct cttgatggac atgtgctaga tgtgttgaaa aacatttcta
4321 agaaaaatgg atttgatttt tttactttaa aagccagtga tgatgatatc tcgcaaatat
4381 taacaaacac agagaaaact cagctatcac cggagtgttc agataaaagt tctttttcgt
4441 tggggtttga atccgagcga agcttactac gactttatcg cgttcaatta cttcttgaac
4501 aggatcaaag cgagtttgtt gcatacacta aattcattaa tgctcaacca gatgagttca
4561 agttaagctt tcaatccttc cgtaatttaa ggaatttctg caagacgcta actgaagaag
4621 aaatagatgt attcagatta actgcaatct taaaaccgtc tcgttttttg atcgaaatat
4681 ataaagattt ggcacctaat ttccctagtg attcggaatt gattctgtca aaaatcacac
4741 aacttaatgc agacggatta aagttcattc ctggtgcatt tgaatttaac gctaatcaag
4801 atgccttact taccaaggct tatcctgtgt atgcacattt aagacatatt attttccaag
4861 aaagtaccac gaaagagcta atacaggggg ttaataatat atcaaaaaat gatatgaata
4921 tgtggaaggc tcgctgggta gctgatttgt gtgcttttga tcgcaatgga tatggcatga
4981 cagagtgtat ctatcaaagg atgcaaacca ttttcaatct agttgaagaa gtacaagaaa
5041 acaaaattga tattcttgaa ttccaagaga aatattataa acatctagat tccatttata
5101 atctagataa gagagtctca aactcaatgc ttgacgaaat agataggaaa atcatttgtc
5161 agctaatcag ttattttgaa aaccctaccg acgaaataat atcaaatatt atatatggat
5221 atgaaagctt tgatggcgac aaaaaagaaa atattaaaaa caattatacc aatataataa
5281 gttgtgaaga gtgtgaggca cctacatact tacctgccgt attgaaaacg gccctatctg
5341 agtttgaaaa gttatctgag gtagaacacc acaatgcgat gtcgttagca acccaatata
5401 cattagattt gttaaatgtt gtatatgaac aattgggttt aaagccagaa aaaatcataa
5461 gtgcgagaga tgcggcttat agtgagaatt ggaaaaaagg caattgggct aaaaaatatt
5521 tagataataa tataaagttt agaataaaaa cagagtcatc atcagttgtt acagaataag
5581 aaacatgatc attattatca tattatttgt aataaggaaa taatatattt aaaatatatc
5641 ggcacaaggg ttagacatct tgaatagagt taaacggctt tgttgcggta atcgatggaa
5701 ctaaattcaa agcttacgat ttgatcggct acgttcattc aactctgcag tctcatgcct
5761 aaaccacgtt acaaaacaac aactggaagc aatacaatca agctctcatc aaccgcggct
5821 ctgtgacgtt ttggattgat gaagaagcga taagagagtg gaaacaaggc aagcaaaaca
5881 agcgcgggag acctcgtatt ttcagtgatt tggccatcac aaccgctttg atggtaaagc
5941 gcattttttc aatgccactt cgccgttcgc aagggtttat ggatcccgtg tttcgacttg
6001 ctcaacttcc actgcgctac ccacactaca gttgtatcag tcgtcgagcc aaagacgttg
6061 atgtaagttt taaaacgaaa accaaaggac cgattcacat cttgctatcg atgcggcagg
6121 tctcaaggtt tatggtgggg gcgagtggaa agtcaaaaaa catggcacgg atggtaaacg
6181 cagagtgtgg cggaaattac atctcgcagt agatacgagt acgcacgaaa ttattgccgc
6241 agagttgagt ttatcaacgg tgagtgaggc agaagtgctg cctaatttgc ttaagcaaac
6301 acgtcgaaaa atccttgaga tatcaggcga tggggcttat gacacgaggg attgccacgc
6361 agcaatccgt attagacgaa ccgtggctct tatccctcca agagagggag cggcattctg
6421 ggaaagaggt catccgagaa atggttgcca aaagctatat ggttcgaaca agcattggaa
6481 aaagcgctat ggctaccaca agcgctcgct atcagaaacg gcgatgtatc gagtcaaaca
6541 gttgcttggt ggacggctaa gtctaagaaa ttacaacgca caagttggtg aaacttacgc
6601 gatgatcaaa gcgttgaaca agcttacagg cttggtatgc ctaaaacttg tcgtattggc
6661 tgacaattgg ctgttcggct tcgaaaatga tttacgcaac aacgccgata ttatcatata
6721 ctggattttt ttattaaatg acaggagtaa atatgtcttt gttttctgta ccatctccta
6781 ttcgggggat gttaaaaatg aatttatcat ctcgag
//