GenBank-Updates@genbank.bio.net (04/24/91)
LOCUS AFAPHBS 1499 bp ds-DNA BCT 24-APR-1991
DEFINITION Alcaligenes eutrophus poly(3-hydroxybutyrate) synthase (phbC) gene,
5' end.
ACCESSION M64341
KEYWORDS poly(3-hydroxybutyrate) synthase.
SOURCE Alcaligenes eutrophus DNA.
ORGANISM Alcaligenes eutrophus
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Aerobic rods and cocci; Alcaligenaceae.
REFERENCE 1 (bases 1 to 1499)
AUTHORS Schubert,P., Krueger,N. and Steinbuechel,A.
TITLE Molecular analysis of the Alcaligenes eutrophus
poly(3-hydroxybutyrate) biosynthetic operon: Identification of the
N terminus of poly(3-hydroxybutyrate) synthase and identification
of the promoter
JOURNAL J. Bacteriol. 173, 168-175 (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
-35_signal 501..506
/gene="phbC"
-10_signal 525..530
/gene="phbC"
mRNA 536..>1499
/gene="phbC"
RBS 826..832
/gene="phbC"
CDS 843..>1499
/product="poly(3-hydroxybutyrate) synthase"
/gene="phbC"
/codon_start=843
BASE COUNT 270 a 500 c 486 g 243 t
ORIGIN
1 cccgggcaag taccttgccg acatctatgc gctggcgcgc acgcgcctgg cgcgcgccgg
61 ctgtaccgag gtctacggcg gcgacgcctg caccgtggcc gacgccggtc gcttctactc
121 ctatcggcgc gatggcgtga ccggccgcat ggccagcctg gtctggctgg cggactgagc
181 ccgccgctgc ctcactcgtc cttgcccctg gccgcctgcg cgcgctcggc ttcagccttg
241 cgtcggcggc ggccgggcgt gcccatgatg tagagcacca gcgccaccgg cgccatgcca
301 tacatcagga aggtggcaac gcctgccacc acgttgtgct cggtgatcgc catcatcagc
361 gccacgtaga gccagccaat ggccacgatg tacatcaaaa attcatcctt ctcgcctatg
421 ctctggggcc tcggcagatg cgagcgctgc ataccgtccg gtaggtcggg aagcgtgcag
481 tgccgaggcg gattcccgca ttgacagcgc gtgcgttgca aggcaacaat ggactcaaat
541 gtctcggaat cgctgacgat tcccaggttt ctccggcaag catagcgcat ggcgtctcca
601 tgcgagaatg tcgcgcttgc cggataaaag gggagccgct atcggaatgg acgcaagcca
661 cggccgcagc aggtgcggtc gagggcttcc agccagttcc agggcagatg tgccggcaga
721 ccctcccgct ttgggggagg cgcaagccgg ttccattcgg atagcatctc cccatgcaaa
781 gtgccggcca gggcaatgcc cggagccggt tcgaatagtg acggcagaga gacaatcaaa
841 tcatggcgac cggcaaaggc gcggcagctt ccacgcagga aggcaagtcc caaccattca
901 aggtcacgcc ggggccattc gatccagcca catggctgga atggtcccgc cagtggcagg
961 gcactgaagg caacggccac gcggccgcgt ccggcattcc gggcctggat gcgctggcag
1021 gcgtcaagat cgcgccggcg cagctgggtg atatccagca gcgctacatg aaggacttct
1081 cagcgctgtg gcaggccatg gccgagggca aggccgaggc caccggtccg ctgcacgacc
1141 ggcgcttcgc cggcgacgca tggcgcacca acctcccata tcgcttcgct gccgcgttct
1201 acctgctcaa tgcgcgcgcc ttgaccgagc tggccgatgc cgtcgaggcc gatgccaaga
1261 cccgccagcg catccgcttc gcgatctcgc aatgggtcga tgcgatgtcg cccgccaact
1321 tccttgccac caatcccgag gcgcagcgcc tgctgatcga gtcgggcggc gaatcgctgc
1381 gtgccggcgt gcgcaacatg atggaagacc tgacacgcgg caagatctcg cagaccgacg
1441 agagcgcgtt tgaggtcggc cgcaatgtcg cggtgaccga aggcgccgtg gtcttcgag
//