GenBank-Updates@genbank.bio.net (04/25/91)
LOCUS ECOPATESPE 4341 bp ds-DNA BCT 25-APR-1991 DEFINITION E.coli ornithine decarboxylase (speF) and putrescine transport protein (patE) genes, complete cds. ACCESSION M64495 KEYWORDS ornithine decarboxylase; patE gene; polyamine transport protein; putrescine transport protein; speF gene; transmembrane protein. SOURCE E.coli (strain DR112) DNA, clone pPT71. ORGANISM Escherichia coli Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1 to 4341) AUTHORS Kashiwagi,K., Suzuki,T., Suzuki,F., Furuchi,T., Kobayashi,H. and Igarashi,K. TITLE Coexistence of the genes for putrescine transport protein and ornithine decarboxylase at 16 min on Escherichia coli chromosome JOURNAL Unpublished (1991) STANDARD full staff_entry FEATURES Location/Qualifiers CDS 660..2858 /product="ornithine decarboxylase" /gene="speF" /codon_start=660 CDS 2855..4174 /product="putrescine transport protein" /gene="patE" /codon_start=2855 BASE COUNT 1076 a 1008 c 1061 g 1196 t ORIGIN 16 min. on K-12 map. 1 ggatcccgcc cgcccacgcc atttcattac tgaaaccggt attggctatc ggtttatgct 61 ttgaatatta attttaatac agcctgcctt ttattaatta aagccgtaat aataaatacg 121 gctttttatc ttaaacaaca cacaaaaata acaattcaat attttatatt actgagtaaa 181 aagctcatca ttaaataaat taagattgat cattttttat tgatcacctt cacagttcaa 241 ccgtatttcc tggatagaat atcttcacct tcattcacat caggaaaggt aaattaaatg 301 gaaaataaca gccgcactat gccccatata aggcggacaa ctcatattat gaagtttgct 361 catcgcaata gcttcgactt tcacttcttc aatgcccgtt agtctaccga ctaagggcac 421 ttcagcgtac aggtcttcct gactctctgt attacaggca tatgcctgta ttccgcagtg 481 ctcattctct gttaaccctt gtattatcag ccacctgact ggtaagggtt tctgcattct 541 gaaaaaagca atctacctga tttatttatc agcggggtaa ctttgctttt ttccggcacg 601 atcgatttct cattcgagaa attgaggacc tgctattacc taaaataaag agatgaaaaa 661 tgtcaaaatt aaaaattgcg gttagtgatt cttgcccgga ctgttttacc acgcagcgag 721 aatgtatcta cattaatgaa agtcgtaata tcgatgtggc ggcaatagtt ttatcgctca 781 acgatgttac atgcggaaaa ctcgatgaaa tcgatgccac gggttatggc atcccggtat 841 ttattgctac tgaaaatcaa gaacgtgtac ccgcagagta tttgccccgt atttcgggtg 901 tctttgagaa ttgcgaatcg cgacgagaat tttatggtcg ccagttagaa accgctgcca 961 gccattatga aactcaactg cgcccacctt tcttccgcgc actggtcgat tatgtcaatc 1021 aaggtaacag cgcgtttgat tgccctggtc atcagggcgg cgaatttttc cgtcgccatc 1081 cggcggggaa tcagtttgtg gaatactttg gtgaggcgct gttccgtgcc gacttgtgca 1141 acgccgacgt agcgatgggc gatctgctga ttcacgaagg cgcgccatgc attgcacagc 1201 aacatgcggc aaaagtgttt aatgccgata aaacctactt cgttttaaat ggcacttcat 1261 cttctaacaa agtggtttta aacgccctgc taacaccggg tgatctggtg ctgtttgatc 1321 gcaataacca caaatctaac caccacggag cgttgctaca ggctggtgca acaccggttt 1381 atctggaaac ggcacgtaac ccgtatggct ttatcggtgg cattgatgcg cactgttttg 1441 aagaaagtta cctgcgtgag ctgatcgcgg aagtcgcacc gcagcgggca aaagaggctc 1501 gtcctttccg cctcgctgtg attcagttag gcacctacga cggtacgatt tataacgccc 1561 gccaagtggt ggataaaatt ggtcatctgt gtgactacat cctgtttgac tcagcatggg 1621 tcggctatga acagtttatt ccgatgatgg cggactgttc gccgctgttg ctggatctta 1681 atgagaacga tccgggtatt ctggttacgc aatctgtgca taaacaacag gctggttttt 1741 ctcagacttc acaaattcat aaaaaagaca gccacatcaa agggcaacag cgttatgtac 1801 cgcacaaacg catgaacaac gcctttatga tgcacgcctc caccagcccg ttctatccgc 1861 tgtttgccgc actgaatatc aacgccaaaa tgcatgaagg tgtcagcggt cgtaatatgt 1921 ggatggattg tgtggtaaat ggcattaatg cccgcaaact gatcctcgat aactgtcagc 1981 atattcgtcc gttcgtacct gaactggtgg atggtaaacc ctggcagtcg tatgaaacag 2041 cgcaaattgc ggttgatctg cgcttcttcc agtttgtacc aggggaacac tggcattctt 2101 ttgaaggcta tgcagagaat caatactttg tcgatccatg caaactgttg ctgacaaccc 2161 caggtattga tgcacgtaac ggcgaatatg aagcgttcgg tgtacccgcg acgattcttg 2221 ctaacttcct gcgcgaaaat ggcgtagtgc cggaaaaatg cgatcttaac tccatcctct 2281 tcctgctgac tccggcagaa gatatggcca aacttcagca acttgttgcc ctgctggtac 2341 gcttcgaaaa actgcttgag tccgacgcgc cattagcaga agtgctacct tccatctaca 2401 aacagcatga agagcgctac gccggttata ccctgcgtca gttgtgtcag gaaatgcatg 2461 atttgtatgc ccgccacaac gtgaaacaac tgcaaaaaga gatgttccgt aaggagcact 2521 tcccacgcgt cagcatgaat ccgcaagaag ccaactacgc ctatttacgc ggtgaagtgg 2581 aactggttcg tctgccggat gcagaaggcc gtatcgctgc cgaaggtgcg cttccttatc 2641 ctccgggtgt gctgtgtgtt gttccgggtg aaatctgggg tggtgctgtt ctgcgttact 2701 tcagcgctct ggaagaaggg atcaacctgc tgccaggttt tgcaccggag ctgcagggtg 2761 tctatatcga agaacatgat ggtcgtaagc aagtttggtg ctatgtcatc aagcctcgtg 2821 atgcgcaaag caccctgttg aaaggggaaa aattatgagt caggctaaat cgaacaaaat 2881 gggcgtcgtt cagttaacca tactgacgat ggtcaacatg atgggctccg gtatcatcat 2941 gctgccgaca aagcttgccg aagtcgggac aatctcaatt atctcctggc tggtgacagc 3001 cgtaggctca atggcactgg catgggcatt cgcaaaatgc ggtatgttca gccgtaaatc 3061 aggcggtatg ggcggttatg ccgagtatgc tttcggtaaa tccggtaact ttatggcgaa 3121 ctatacctac ggcgtctcac tgctgattgc taacgtcgcg attgctattt cggcggttgg 3181 ttacggcacc gaactgctcg gcgcgagttt gtcgccagtg cagattggtc ttgcgaccat 3241 cggggtgctg tggatttgta ccgtggctaa ctttggtggt gcgcgcatta ccgggcaaat 3301 cagtagcatt accgtgtggg gggtcattat tccggtcgtc ggtctgtgca ttattggctg 3361 gttctggttt agcccgacgc tgtacgttga ttcctggaat ccgcatcatg caccgttctt 3421 cagtgcggta ggttcttcca tcgctatgac gctgtgggct tttcttggtc tggagtctgc 3481 gtgtgcgaat actgatgtag tggaaaaccc ggaacgtaat gtgccaatcg cggtactcgg 3541 cggtacgtta ggtgcggcgg tgatttatat cgtctccacc aacgtgattg ccgggattgt 3601 gccaaatatg gagctggcaa attcaacggc accatttggt ctggccttcg cgcagatgtt 3661 cacgccggaa gtgggtaaag tcattatggc gctgatggtg atgtcctgct gcggttcgct 3721 acttggctgg cagttcacca ttgcccaggt gtttaaatct tcatctgatg aaggctactt 3781 ccctaaaatt ttctcccgtg taaccaaagt ggatgcaccg gtgcagggaa tgttgaccat 3841 tgtgattatt cagagtggat tggcactgat gaccattagc ccgtcgctaa acagtcagtt 3901 caacgtgctg gttaacctgg ccgtggtgac caatatcatt ccgtatattc tgtcaatggc 3961 ggcattagtc attattcaga aggtcgctaa tgtgcccccc tcaaaagcga aagttgcaaa 4021 ctttgttgct tttgttggcg cgatgtatag cttttatgcg ctgtactcat ccggggaaga 4081 agccatgctg tacggttcca tcgtgacctt ccttggttgg acactgtatg gtctggtatc 4141 accacgcttt gaactgaaaa ataaacacgg ttaataaaac aaagtaaaaa agggcgattg 4201 aatgatcgcc ctttctgttt gttggcaaat agttcgttat ttatgccgca tccggcagat 4261 gtagggtcta aacgcgatcg aacacagcaa ctatttgcgt aattatatgg ggcatctgcg 4321 acaaaaactg gaacaggatc c //