GenBank-Updates@genbank.bio.net (04/25/91)
LOCUS ECOPATABCD 4385 bp ds-DNA BCT 25-APR-1991
DEFINITION E.coli transport protein (patA, patB, patC and patD) genes,
complete cds.
ACCESSION M64519
KEYWORDS patA gene; patB gene; patC gene; patD gene;
periplasmic bindin protein; polyamine transport protein;
putrescine transport protein; spermidine transport protein;
transmembrane protein.
SOURCE E.coli (strain DR112) DNA, clone pPT104.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 4385)
AUTHORS Furuchi,T., Kashiwagi,K., Kobayashi,H. and Igarashi,K.
TITLE Characteristics of the gene for spermidine and putrescine transport
system mapped at 15 min on Escherichia coli chromosome
JOURNAL J. Biol. Chem. (1991) In press
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 379..1515
/product="transport protein"
/gene="patA"
/codon_start=379
CDS 1529..2356
/product="transport protein"
/gene="patB"
/codon_start=1529
CDS 2353..3147
/product="transport protein"
/gene="patC"
/codon_start=2353
CDS 3144..4190
/product="transport protein"
/gene="patD"
/codon_start=3144
sig_peptide 3144..3212
/gene="patD"
/codon_start=3144
mat_peptide 3213..4187
/product="transport protein"
/gene="patD"
/codon_start=3213
BASE COUNT 1068 a 1059 c 1125 g 1133 t
ORIGIN 15 min. on K-12 map.
1 ttcgagctgc tctttcagca gatgcaataa cttccattgg ccttccgtgc tgggaacctg
61 tctcacccca gcttttgatt gggtatccag agacacgtag ttcaaaaatc gctcaagtag
121 tttatccatg tagtcaccct cactttttgt gacaacatta ttaagaagca agaaaagaca
181 aatattgcgt caggtcactt ttacccctgc aagcgggaat atttatcagc attaccttaa
241 tgaatataaa gctaagccag taattcacaa caaagattgg cgattcaagc ggtttgccgt
301 agaattaccg ccctcattaa gagtcaccaa ggtggttaac cacaaacccc gcatcggtaa
361 gccatccgtt gcgtttacat gggacagagt aaaaaattga ataaacaacc gagttcgctt
421 tcaccgctgg tgcaattggc gggaattcgc aaatgctttg atggtaaaga ggtcattccc
481 cagctggatc tgactatcaa caatggcgag ttcctcacgc tgcttggccc ttctggctgc
541 ggtaaaacaa ccgttcttcg cctgattgca ggtctggaaa ctgttgattc cggacgcatc
601 atgctggata acgaggacat cacccacgtt ccggcggaaa accgctatgt gaacactgtt
661 ttccaaagct acgcactttt cccccacatg accgtgttcg aaaatgtggc ctttgggttg
721 cgcatgcaaa aaacccccgc tgctgaaatt acgccccgcg tgatggaagc cctgcggatg
781 gtgcagttgg aaaccttcgc tcaacgcaaa ccgcatcagc tctctggtgg tcaacagcaa
841 cgcgtcgcca ttgctcgcgc ggtggttaac aagcctcgtc tgttgttgct ggatgagtcg
901 ctctcagcgc tggattacaa actgcgtaag caaatgcaga acgagctgaa agcgttacag
961 cgtaagcttg gcattacatt cgtctttgtg actcacgacc aggaagaagc actcaccatg
1021 tcagacagga ttgtggtgat gcgcgatggt cgcattgagc aagacggcac gccgcgtgaa
1081 atctacgaag agccgaaaaa cctgtttgtt gccggcttca ttggcgaaat caatatgttt
1141 aacgccactg tcatcgaacg tctagacgag cagcgcgtac gcgccaacgt tgaaggccgc
1201 gaatgtaata tctacgttaa cttcgccgtt gaaccggggc aaaaactgca tgttctgctg
1261 cgcccggaag acttacgtgt tgaagagatt aacgacgaca accacgctga agggctgatt
1321 ggttacgttc gcgagcgtaa ctacaaaggc atgacgctgg agtcggttgt tgaactggaa
1381 aatggcaaga tggtgatggt cagcgaattc ttcaatgaag acgatcctga ctttgaccac
1441 tctctcgacc aaaaaatggc cattaattgg gtagaaagct gggaggtcgt actggctgat
1501 gaagaacaca agtaagttcc agaatgtagt gattgtcact attgtcggtt ggcttgtgtt
1561 gtttgtcttt ctgcccaacc tgatgatcat tggcaccagc tttttgaccc gcgacgacgc
1621 cagttttgtc aaaatggtct ttacgctgga taactacacg cgtctgctcg atccgctcta
1681 ttttgaagtg ctattgcact cgctgaatat ggcgctgatc gccaccctcg cctgcctggt
1741 gctgggctac ccgtttgcct ggtttctggc gaagttgcca cacaaggtgc gtccgctgct
1801 gctgtttctg ctgattgttc cgttctggac caactcatta attcgtatct acgggctgaa
1861 aattttcctc agcaccaaag gctatctcaa cgagtttttg ctctggctgg gcgttatcga
1921 cacaccaatc cgtatcatgt tcacgcccag tgcggtgatt atcggtctgg tttacattct
1981 gctgccgttt atggtgatgc cgctgtactc cagtatcgaa aaactggata agccgttgct
2041 tgaggcggcg cgcgatctcg gtgccagcaa gttacagact tttatccgta tcattattcc
2101 actgacgatg ccgggaatta ttgccggatg tctgctggtg atgctgccag cgatgggcct
2161 gttctatgta tccgacctga tgggcggcgc gaaaaacctg ctgatcggta acgtcatcaa
2221 ggtccagttc cttaatattc gtgactggcc gtttggtgca gctaccagca ttacgctgac
2281 tatcgtaatg ggcctgatgt tgctggttta ctggcgcgct tctcgtttgc tgaataagaa
2341 ggtggaactc gaatgatcgg tcgactgctt cgcggcggtt ttatgaccgc tatctacgcg
2401 tacctgtata tcccaatcat tattttgatt gtgaactcct ttaacagctc gcgctttggc
2461 atcaactggc agggctttac caccaaatgg tatagcctgc tgatgaacaa cgacagcctg
2521 ttacaggcag cgcagcattc actaacaatg gcggtgtttt cggcgacgtt tgctacgctt
2581 atcggttcac tgacggcagt tgcgctgtac cgttatcgct ttcgtggtaa gccgttcgtt
2641 agcggaatgc tgtttgtggt gatgatgtca ccagatatcg tgatggcgat ttcgctgctg
2701 gtgctgttta tgctgctggg tattcagctt ggcttctggt cgctgctgtt ctcgcatatc
2761 accttctgcc tgccatttgt ggtggtgacg gtgtattcgc gcctgaaagg ttttgacgtg
2821 cgaatgctgg aagcggcgaa agatctcggt gccagcgaat ttaccattct gcgaaaaatc
2881 attctgccac tggcaatgcc agcggtggcg gcgggctggg tgttaagctt taccctgtcg
2941 atggacgatg tggtagtttc ttcgttcgtc accggaccaa gttatgaaat tctgccgtta
3001 aaaatttatt cgatggtcaa agtcggcgta tcgccggaag ttaacgcgct ggcaaccata
3061 ttactggtgc tgtcgctggt gatggttatt gccagccagc ttattgctcg tgataaaacg
3121 aaaggtaaca caggggacgt taaatgaaaa aatggtcacg ccacctgctc gcggcgggtg
3181 ctctggcact gggcatgagc gccgctcacg ccgatgacaa caacacgctg tatttctaca
3241 actggaccga gtacgtgccg ccaggactgc ttgaacagtt caccaaagaa accggtatta
3301 aggttatcta ttcgacttac gagtcgaacg aaaccatgta cgccaagctg aaaacataca
3361 aagacggtgc ctatgatctg gtggttcctt caacctatta cgttgataaa atgcgtaaag
3421 aagggatgat ccagaagatc gacaagtcga agttaacaaa cttcagcaat ctcgatccag
3481 acatgctcaa caagcctttt gacccgaata acgactactc cattccgtat atctggggtg
3541 cgacggcgat tggtgttaac ggtgatgcgg tggatccgaa atctgtcacc agctgggccg
3601 atctgtggaa gccagagtac aaaggcagcc tgctgttgac cgacgatgcc cgtgaagtgt
3661 tccagatggc gctgcgtaag ctgggctact ccggtaacac caccgatccg aaagagattg
3721 aagctgcata taacgagctg aaaaaactga tgccaaacgt ggcagccttt aactccgata
3781 acccggcgaa cccgtacatg gaaggcgaag ttaacctcgg catgatctgg aacggttctg
3841 ctttcgttgc acgccaggcg ggtacgccaa ttgacgtggt gtggccgaaa gaaggcggca
3901 ttttctggat ggacagcctg gcgatcccgg caaatgccaa aaacaaagaa ggcgcgctga
3961 aattgatcaa cttcctgctg cgcccggatg tggcaaaaca ggttgctgaa actatcggtt
4021 atccaacgcc aaaccttgcg gcgcgtaagc tgttaagtcc agaagtggcg aacgataaaa
4081 cactctaccc ggatgctgaa accattaaaa atggcgaatg gcagaatgac gttggcgcag
4141 ccagcagcat ttatgaagag tattatcaga agctgaaagc aggacgttaa ttcagcaagc
4201 gggcggtggt attaccgccc gttttttagt tagcaaggat gtggctaatg agtcaggaca
4261 gtaaagtttt ctttcgtata ttcctcggta tcggtctggt actgatcctc atttctgttg
4321 ttgtctttta taaccagttc acctatgcaa agacgcgatt cataccgaag gcgtcattgt
4381 tgata
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