GenBank-Updates@genbank.bio.net (04/04/91)
LOCUS BACCEL 1612 bp ds-DNA BCT 04-APR-1991
DEFINITION Bacillus sp. cellulase gene, complete cds, clone pNK2.
ACCESSION M14729
KEYWORDS cellulase.
SOURCE Bacillus sp. (alkalophyllic; strain N-4 (ATCC 21833)) DNA, clone
pNK2.
ORGANISM Bacillus sp.
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 1612)
AUTHORS Fukumori,F., Sashihara,N., Kudo,T. and Horikoshi,K.
TITLE Nucleotide sequences of two cellulase genes from alkalophilic
Bacillus sp. strain N-4 and their strong homology
JOURNAL J. Bacteriol. 168, 479-485 (1986)
STANDARD full staff_review
COMMENT A region of dyad symmetry, which could be a transcription
termination signal, is located at positions 1393-1431.
FEATURES Location/Qualifiers
CDS 129..1358
/note="cellulase (EC 3.2.1.4)"
/codon_start=129
BASE COUNT 545 a 269 c 365 g 433 t
ORIGIN 1 bp upstream of EcoRI site.
1 gaattccgca acacattttt tttggaagag ggtgatagcg tttacatata ctccttaaaa
61 agtgcaagcg cagactaaaa cgatttcgtt tcagtatgaa aagctaaaac attaccgagg
121 aggaaaatat gaaaaagata actactattt ttgtcgtatt gctcatgaca ctggcattgt
181 tcattatagg aaacacgact gctgctgatg attattcagt tgtagaggag catgggcaat
241 taagtattag taacggagaa ttagtcaatg atcgaggcga accagttcag ttaaaaggga
301 tgagttccca tggtttacaa tggtacggtc aatttgtaaa ctatgaaagc atgaaatggc
361 taagagatga ttggggtata actgtattcc gagcagcgat gtatacatct tcgggaggat
421 atattgagga tccttccgta aaggaaaaag taaaagaggc tgttgaggct gcgatagacc
481 ttggtatata tgtcataatt gattggcaca tcctttcaga caatgacccg aatatatata
541 aagaagaagc aaaggatttc tttgatgaaa tgtctgagct gtatggagat tacccgaatg
601 tgatatacga aattgcaaat gaaccgaatg gtagtgatgt tacgtgggac aatcaaataa
661 aaccgtatgc agaggaagta attccggtta tccgtaacaa tgatcctaat aacattatta
721 ttgtaggtac aggtacatgg agtcaggatg ttcatcatgc tgctgataat cagttaacag
781 atccgaacgt catgtatgca tttcattttt atgcaggaac acatggacaa aatttacgag
841 accaagtaga ttatgcatta gatcaaggag cagcaatatt tgttagtgaa tggggaacga
901 gtgaagctac tggtgatggc ggcgtgtttt tagatgaagc acaagtgtgg attgacttta
961 tggatgaaag aaatttaagc tgggcaaact ggtctctaac gcacaaagat gagtcatctg
1021 cggcgttaat gccaggtgca agcccaactg gtgggtggac agaggctgaa ctatctccat
1081 ctgggacatt tgtgagggaa aaaataagag agtcagcaac aacaccacct agtgatccaa
1141 caccaccatc tgatccagat ccaggtgaac cagaaccaga tccaggtgaa ccggatccaa
1201 cgccaccaag tgatccagga gattatccgg catgggatcc aaatacaatt tatacagatg
1261 aaattgtgta ccataacggc cagctatggc aagcaaaatg gtggacgcaa aatcaagagc
1321 caggcgaccc atacggtccg tgggaaccac tcaattaacg atataatgat agaaatttac
1381 taatgatata aggagaatgc caagagtcta aattggacga ttggcattct cattttacat
1441 attagagttg aaatactttc cgctattcgt ctgcaataag ttaacccata cttagtagta
1501 gtttccaatc tgtaaaacac ttttaagaaa aggttttctt acgaatcatt tacatgcaaa
1561 ttaataaatt gtgagacatt agtgtaaaac tttttatgta gtagtaaagc tt
//GenBank-Updates@genbank.bio.net (04/25/91)
LOCUS BACCEL 1612 bp ds-DNA BCT 25-APR-1991
DEFINITION Bacillus sp. cellulase gene, complete cds, clone pNK2.
ACCESSION M14729
KEYWORDS cellulase.
SOURCE Bacillus sp. (alkalophyllic; strain N-4 (ATCC 21833)) DNA, clone
pNK2.
ORGANISM Bacillus sp.
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 1612)
AUTHORS Fukumori,F., Sashihara,N., Kudo,T. and Horikoshi,K.
TITLE Nucleotide sequences of two cellulase genes from alkalophilic
Bacillus sp. strain N-4 and their strong homology
JOURNAL J. Bacteriol. 168, 479-485 (1986)
STANDARD full staff_review
COMMENT A region of dyad symmetry, which could be a transcription
termination signal, is located at positions 1393-1431.
FEATURES Location/Qualifiers
CDS 129..1358
/note="cellulase (EC 3.2.1.4)"
/codon_start=129
BASE COUNT 545 a 269 c 365 g 433 t
ORIGIN 1 bp upstream of EcoRI site.
1 gaattccgca acacattttt tttggaagag ggtgatagcg tttacatata ctccttaaaa
61 agtgcaagcg cagactaaaa cgatttcgtt tcagtatgaa aagctaaaac attaccgagg
121 aggaaaatat gaaaaagata actactattt ttgtcgtatt gctcatgaca ctggcattgt
181 tcattatagg aaacacgact gctgctgatg attattcagt tgtagaggag catgggcaat
241 taagtattag taacggagaa ttagtcaatg atcgaggcga accagttcag ttaaaaggga
301 tgagttccca tggtttacaa tggtacggtc aatttgtaaa ctatgaaagc atgaaatggc
361 taagagatga ttggggtata actgtattcc gagcagcgat gtatacatct tcgggaggat
421 atattgagga tccttccgta aaggaaaaag taaaagaggc tgttgaggct gcgatagacc
481 ttggtatata tgtcataatt gattggcaca tcctttcaga caatgacccg aatatatata
541 aagaagaagc aaaggatttc tttgatgaaa tgtctgagct gtatggagat tacccgaatg
601 tgatatacga aattgcaaat gaaccgaatg gtagtgatgt tacgtgggac aatcaaataa
661 aaccgtatgc agaggaagta attccggtta tccgtaacaa tgatcctaat aacattatta
721 ttgtaggtac aggtacatgg agtcaggatg ttcatcatgc tgctgataat cagttaacag
781 atccgaacgt catgtatgca tttcattttt atgcaggaac acatggacaa aatttacgag
841 accaagtaga ttatgcatta gatcaaggag cagcaatatt tgttagtgaa tggggaacga
901 gtgaagctac tggtgatggc ggcgtgtttt tagatgaagc acaagtgtgg attgacttta
961 tggatgaaag aaatttaagc tgggcaaact ggtctctaac gcacaaagat gagtcatctg
1021 cggcgttaat gccaggtgca agcccaactg gtgggtggac agaggctgaa ctatctccat
1081 ctgggacatt tgtgagggaa aaaataagag agtcagcaac aacaccacct agtgatccaa
1141 caccaccatc tgatccagat ccaggtgaac cagaaccaga tccaggtgaa ccggatccaa
1201 cgccaccaag tgatccagga gattatccgg catgggatcc aaatacaatt tatacagatg
1261 aaattgtgta ccataacggc cagctatggc aagcaaaatg gtggacgcaa aatcaagagc
1321 caggcgaccc atacggtccg tgggaaccac tcaattaacg atataatgat agaaatttac
1381 taatgatata aggagaatgc caagagtcta aattggacga ttggcattct cattttacat
1441 attagagttg aaatactttc cgctattcgt ctgcaataag ttaacccata cttagtagta
1501 gtttccaatc tgtaaaacac ttttaagaaa aggttttctt acgaatcatt tacatgcaaa
1561 ttaataaatt gtgagacatt agtgtaaaac tttttatgta gtagtaaagc tt
//