GenBank-Updates@genbank.bio.net (04/25/91)
LOCUS ECOCELA 4989 bp ds-DNA BCT 25-APR-1991
DEFINITION E.coli cellobiose permease proteins celB, celC, celD and celF
genes, complete cds.
ACCESSION M64438
KEYWORDS PTS enzyme II; PTS enzyme III; PTS enzyme IV; celA gene;
cellosbiose phosph-beta-glucosidase.
SOURCE E.coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Parker,L.L. and Hall,B.G.
TITLE Characterization and Nucleotide Sequence of the Cryptic cel operon
of Escherichia coli K12
JOURNAL Genetics 124, 455-471 (1990)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Parker,L.L. and Hall,B.G.
TITLE Mechanisms of Activation of the Cryptic cel operon of Escherichia
coli K12
JOURNAL Genetics 124, 473-482 (1990)
STANDARD full staff_review
REFERENCE 3 (bases 1 to 4989)
AUTHORS Reizer,J. and Saier,M.H.Jr..
TITLE The cellobiose permease of Escherichia coli consists of three
proteins and is homologous to the lactose permease of
Staphylococcus aureus
JOURNAL Res. Microbiol. 141, 1061-1067 (1991)
STANDARD full automatic
FEATURES Location/Qualifiers
misc_feature 59..167
/function="'insertion sequences IS1, IS2, and IS5 insert
and activate the cryptic operon'"
/citation=Genetics 124, 473-482 (1990)
CDS 256..606
/product="PTS enzymeIV cel"
/gene="celA"
/codon_start=256
/citation=Res. Microbiol. 141, 1061-1067 (1991)
/partial
CDS 691..1944
/product="'PTS enzymeII cel'"
/gene="celB"
/codon_start=691
CDS 2089..2439
/product="'PTS enzymeIII cel'"
/gene="celC"
/codon_start=2089
CDS 2447..3289
/product="repressor"
/gene="celD"
/codon_start=2447
mutation 2983..2983
/note="'G' in mutation"
/citation=Genetics 124, 473-482 (1990)
mutation 2995..2995
/note="'G' in mutation"
/citation=Genetics 124, 473-482 (1990)
mutation 2996..2996
/note="'C' in mutation"
/citation=Genetics 124, 473-482 (1990)
CDS 3394..4512
/product="'cellosbiose phosph-beta-glucosidase'"
/gene="celF"
/codon_start=3394
BASE COUNT 1312 a 1032 c 1260 g 1385 t
ORIGIN
1 aagcttgagt aacaacggaa accggccatt gcgccggttt tttttggcct gagttcttaa
61 ttatcttcgc gaattatttg cccgaaatgt gaagagggtc ataaccacag gtcaaggaga
121 aacaatttat aaggtcaaag aaatactatt gctcaggtct ataccgtata ctcctttcag
181 ccacaaaaaa agtcatgttg gtttcgcaag ttacccagag catggaagca ggttaaggct
241 tgcggagtgt ctggctgaca gataatcgtc gatgagggca gttttatgga aaagaaacac
301 atttatctgt tttgttctgc gggcatgtct acctctttac tggtatcaaa aatgcgcgca
361 caggcagaaa aatatgaagt tccggtcatt attgaagcat ttccggaaac actggctggt
421 gaaaaaggtc agaatgccga tgtcgtgtta ttagggccgc agattgctta tatgttgccc
481 gaaatccagc gtttgttacc caacaaaccg gttgaagtaa ttgactcgct gctttatggc
541 aaagtcgatg gtttaggcgt gcttaaggct gcggttgcag cgattaaaaa agccgcagca
601 aattaattta ttttaaattt tcccgtcaaa gagttatttc ataaatcaat accgcaatat
661 ttaaattgcg gtttttaagg gtatttttct atgagtaatg ttattgcatc gcttgaaaag
721 gtactcctcc cttttgcagt taaaatagga aagcagccac acgttaatgc aatcaaaaat
781 ggctttattc gcttaatgcc gttaaccctt gcgggggcca tgtttgtatt aattaacaac
841 gtttttctaa gctttgggga ggggtcgttt ttttattcct taggtattcg cctcgacgcc
901 tcaaccattg aaacacttaa tggtctgaaa ggtattggcg gcaacgtata taacggaaca
961 ttaggaataa tgtctttaat ggcaccgttc tttattggca tggcgctggc agaagagcgt
1021 aaagtcgatg cgctggcggc tgggttgtta tccgttgcag catttatgac cgtcacccca
1081 tatagtgtcg gtgaggccta tgcggttggt gcaaactggt taggtggggc gaatatcatc
1141 tccgggatta ttattggcct ggtggtggca gaaatgttta cctttattgt ccgccgcaat
1201 tgggtcatta aactgcccga cagcgtacct gcttcagtat cgcgttcctt ctcggcattt
1261 aattcccggc tttattattc tttccgtgat ggggattatt gcctggcgtt gaatacctgg
1321 ggcaccaact tccatcagat cattatggat accatctcaa ccccactggc atcgttgggt
1381 agcgtgtggc tggcctatgt gatcttgtcc actgctctgg ttcttcgtat tcatgctgct
1441 tgcgctgacc gcactggaca acggcattat gacgcgtggg cactggaaaa tatcgcgacc
1501 tatcagcaat atggttccgt cgaagcggcg ctggcagccg gtaagacctt ccatatctgg
1561 gccaagccga tgctggactc ctttattttc cttgggggca gtggtgcgac tttaggcctg
1621 atcctggcta tctttatcgc ctctcgccgt gctgattatc gtcaggtggc aaaactggcg
1681 ctgccgtccg gcatcttcca gattaacgaa ccgattctgt ttggtctgcc aattatcatg
1741 aacccggtga tgtttatccc gttgtactgg tacaaccgga ttctggcggc aatcaccctc
1801 gcagcgtact acatgggcat tattcctccg gtgaccaata ttgcaccgtg gaccatgcca
1861 accggtctgg gagccttctt taacaccaac ggtacgtcgc cgcattgctg gtcgcactct
1921 tcaaccttgg catcgcaacg ttaatttatc tgccctttgt tgtggtggct aacaaagcac
1981 aaaatgcgat tgataaagaa gagagcgaag aagatatcgt aacgccctga aattttaatc
2041 gatgccggta cgggtaatcg tgccggtaag aaaagagagg aacgatgtat gatggatctc
2101 gataacattc ccgatacgca aacggaagct gaagagctgg aagaagtggt gatggggctg
2161 atcatcaact ccggacaagc gcgcagcctg gcgtatgcgg cactgaaaca ggcgaagcag
2221 ggcgattttg ccgcagcaaa agccatgatg gatcagtcac ggatggcatt gaatgaagcg
2281 catctggtac agacgaaact gattgaaggc gatgcgggcg aaggtaagat gaaagtgagt
2341 ctggtgctgg tccacgctca ggatcattta atgacgtcca tgcttgcgcg tgaactgatt
2401 actgaattaa ttgagcttca tgaaaaactg aaggcataag gagtcgatga tgcagccagt
2461 gattaacgcg ccggaaattg ccactgcccg agaacagcag ttgtttaatg gcaaaaactt
2521 ccatgtgttt atctataaca aaactgagag tatcagcgga ctgcatcagc acgactatta
2581 tgaatttact ctggtattaa ccgggcgtta tttccaggag attaacggta agcgcgtgtt
2641 actggaacgg ggcgattttg tttttattcc gttaggttcg caccatcaaa gtttttatga
2701 gtttggtgcc acgcgcatat tgaacgttgg gatcagtaaa cgcttttttg agcagcatta
2761 cctgccattg ttgccttatt gctttgtcgc ttcgcaggta taccggacca ataacgcttt
2821 tctcacctat gtggaaacag tgatttcttc attgaatttc cgcgaaacag ggctggaaga
2881 gtttgttgag atggttactt tttatgtcat taaccgttta cgtcattacc gcgaagaaca
2941 ggtgattgat gatgtaccgc agtggctgaa aagtacggta gaaaagatgc atgataaaga
3001 gcagtttagt gaatcggcgc tggagaatat ggtggcgttg tcagccaaat cacaggaata
3061 tttgacgcga gcgactcaac gatattatgg caaaacgcca atgcagatta ttaatgaaat
3121 ccgtattaat tttgccaaaa aacaactgga aatgaccaac tattcagtga cggatattgc
3181 gtttgaggcc ggttatagta gcccgagttt gtttattaaa acgtttaaga aattaacgtc
3241 ctttacgcct aagagctatc gtaagaaatt gactgaattt aatcagtaag tcgttatacc
3301 tgacaattca catatctgtc ctgttgctgg attatttatg tccggggggg cagatatgcc
3361 agatatcagt attctgtact gaagggagaa attatgagcc agaaattaaa agtcgtcact
3421 attggtggcg ggagcagcta taccccggag ttactggaag gatttattaa gcgttatcac
3481 gaattgccgg tcagcgaatt atggctggtg gatgtcgaag gtggtaaacc gaaactggat
3541 attattttcg atctctgcca acggatgatt gataacgctg gcgtcccgat gaagctttat
3601 aaaacgctgg atcgccgcga agcattgaaa gatgctgatt tcgttactac ccaactgcgc
3661 gttggccaat taccggcgcg tgaactggat gaacgtattc cattaagtca tggttatctt
3721 ggtcaggaaa ccaacggcgc gggcggtttg tttaaaggtc tgcgtaccat tccggtgatt
3781 tttgacatcg taaaagatgt cgaagaactt tgtccgaatg catgggtgat taacttcact
3841 aacccggcgg gaatggtcac tgaagccgtt tatcgtcata ccggatttaa acgctttatc
3901 ggcgtgtgta atattccgat cggcatgaag atgtttattc gcgatgttct gatgctgaaa
3961 gacagcgatg atttatctat cgatttgttc ggcctcaacc atatggtgtt cattaaggat
4021 gtgctgataa atggcaagtc gcgctttgcc gaattgcttg atggtgtggc gtcagggcag
4081 ttaaaagcgt cctctgtaaa aaatattttc gatctgccat ttagtgaggg cttaattcgt
4141 tcgttgaatc tgctgccatg ttcttatctg ctgtattact tcaagcagaa agagatgctg
4201 gctattgaaa tgggcgaata ctacaaaggc ggcgcacgag cgcaggtagt acagaaagtc
4261 gagaaacaac tttttgagct gtataaaaat cctgagctga aagttaagcc gaaagaactg
4321 gaacagcgcg gtggggctta ttactctgat gcagcatgcg aagtgatcaa cgctatctac
4381 aacgacaagc aagcagaaca ttacgttaat atcccgcatc atgggcagat tgataatatt
4441 ccggcagact gggcagtaga aatgacctgt aagctggggc gcgatggcgc gacgccacat
4501 ccgcgcaatt aggcatttcg atgataaagt aatggggctg ttcacaccat taaaggcttc
4561 aagattgctg ccagtaacgc cgcaacttaa cggagaattg aacgatatgt tactggcgct
4621 aaaccttagt ccgttggtgc attccgatcg cgatgctgag ctgctggcac gcgagatgat
4681 tctggcgcac gagaaatggc tgccaaactt tgccgactgc atcgcagagc ttaaaaaagc
4741 acattaaccg aggctgatta tggaacgctt actgcttgtt aatgccgatg attttggctt
4801 aagtcaaagg acagaactac ggcattatcg aggcctgtcg caatgggatt gtcactgtcg
4861 acgacgtcac tgtgaatggc aggctattga ccatgcggtg catttgagtt gtgatgaacc
4921 aattctggcc atagggatgc actttgtcct tattatgggt aagccactga cagctatgcc
4981 ggggttaac
//