GenBank-Updates@genbank.bio.net (04/04/91)
LOCUS BACCELA 2088 bp ds-DNA BCT 04-APR-1991
DEFINITION Bacillus sp. cellulase gene, complete cds, clone pNK1.
ACCESSION M14781
KEYWORDS cellulase.
SOURCE Bacillus sp. (alkalophyllic; strain N-4 (ATCC 21833)) DNA, clone
pNK1.
ORGANISM Bacillus sp.
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 2088)
AUTHORS Fukumori,F., Sashihara,N., Kudo,T. and Horikoshi,K.
TITLE Nucleotide sequences of two cellulase genes from alkalophilic
Bacillus sp. strain N-4 and their strong homology
JOURNAL J. Bacteriol. 168, 479-485 (1986)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Nakamura,A., Fukumori,F., Horinouchi,S., Masaki,H., Kudo,T.,
Uozumi,T., Horikoshi,K. and Beppu,T.
TITLE Construction and characterization of the chimeric enzymes between
the bacillus subtilis cellulase and an alkalophilic bacillus
cellulase
JOURNAL J. Biol. Chem. 266, 1579-1583 (1991)
STANDARD full staff_review
COMMENT A region of dyad symmetry, which could be a transcription
termination signal, is located at positions 1665-1697.
FEATURES Location/Qualifiers
CDS 192..1658
/note="cellulase (EC 3.2.1.4)"
/codon_start=192
repeat_region 1212..1391
/note="repeat copy A"
repeat_region 1476..1652
/note="repeat copy B"
BASE COUNT 694 a 381 c 448 g 565 t
ORIGIN 1 bp upstream of HindIII site.
1 aagcttattt tattgattag gtatattcac tgattgaata aagagagttt gttttataaa
61 attaacaacg aaaaaaccat tttcgttcgg gaggagttga tagcgcttac aattcgctag
121 tttataagtg tgtttagaaa ctaaaacgat ttcgtctcta aagtctgtaa aaaaaatttt
181 aggaggaaaa gatgaaaaaa ctaacgacta tttttattgt atttacgtta gcattattat
241 ttgttggaaa ctctacttct gcaaataatg gttccgttgt tgagcaaaat ggacaattaa
301 gtattcaaaa cggtcaatta gtgaatgaac atggagaccc ggtacagcta aagggaatga
361 gttcacatgg attacaatgg tatggacaat ttgtaaacta cgatagtata aagtggttaa
421 gagacgattg gggaataacc gtattccgag cagcgatgta tacatcttca ggagggtata
481 ttgaagatcc ttcagtaaaa gaaaaagtaa aagaggctgt tgaagctgcg attgatctcg
541 gaatatatgt cattattgac tggcatattc tttctgataa tgacccgaac atttataagg
601 aagaagcaaa agagtttttt gatgaaatgt ctgcacttta tggggattat ccgaatgtca
661 tttatgagat tgcgaatgaa ccaaacggac ataatgtaag atgggatagc catattaaac
721 catatgcaga ggaagtaatc ccagtaatcc gtgcaaacga tcctaataat attgtcattg
781 ttgggacagc aacttggagt caggatgtac acgaagcagc tgataaccaa ctagatgacc
841 cgaatgtaat gtatgcgttc cacttctacg ctggaacaca tggtcagcaa ttaagaaatc
901 aagttgacta tgctttaagt cgaggagcag cgatattcgt tagtgagtgg ggaacgagtg
961 cggctacggg tgatggtggc gtatttttag atgaagccca agtgtggatt gactttatgg
1021 atgagagaaa tttaagctgg gcaaactggt ctttaacaca taaagatgag tcatctgcag
1081 cgttaatgcc tggtgcaaac ccaactggtg ggtggacagc ggctgaattg tctccatctg
1141 gtgcatttgt gagggaaaaa ataagagaat cagcgtctat tccgccaagc gatccaacac
1201 ctccatctga tccagatcca ggtgagccgg acccaacgcc accaagtgat ccaggggagt
1261 atccagcatg ggatccaaat caaatttaca caaatgaaat tgtgtatcat aacggtcagt
1321 tatggcaagc gaaatggtgg acacaaaatc aagagccggg agctaatcaa tatgggccat
1381 gggagccttt aggagatgct cctccaagtg aaccgagcga tccaccacca ccatcagaac
1441 cagagccgga cccaggagaa ccagatccag gtgagccaga cccaggagaa ccggatccaa
1501 cgccaccaag tgatccagga gagtatccag catgggaccc aacgcaaatt tacacgaatg
1561 aaattgttta ccataacgga caattgtggc aagcaaaatg gtggacacaa aatcaagaac
1621 ccggatatcc ttatggacca tgggagccgt taaactaaca tattaatcat aaatgaaaag
1681 gtgctcagca aagaggagca ccttttctaa caaaacttta gtccttcgct ctaacaagag
1741 gcatagtaca acatctaaaa gtaccaccgg acttaataat ttctgtaata tcgacttcta
1801 tcacttcata accacgaata cgaagctgct gattgacttc tctatttata ggtaagctaa
1861 caattctttt tttaccaata gaaagtacgt ttgttccaag tgtgaactgc tcttcttcat
1921 ttacttcaat taaatcaaaa tgctttgcta atatcttttc ttcctttttg tcaaaagctc
1981 gtgggaaata taacgcttca ttctctgaaa taatatgaac acacaatcta agtgtaaata
2041 tttttcagta aaaggtacgg ttattacttc gtactcgtgt aaaagctt
//GenBank-Updates@genbank.bio.net (04/25/91)
LOCUS BACCELA 2088 bp ds-DNA BCT 25-APR-1991
DEFINITION Bacillus sp. cellulase gene, complete cds, clone pNK1.
ACCESSION M14781 X53449
KEYWORDS cellulase.
SOURCE Bacillus sp. (alkalophyllic; strain N-4 (ATCC 21833)) DNA, clone
pNK1.
ORGANISM Bacillus sp.
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 2088)
AUTHORS Fukumori,F., Sashihara,N., Kudo,T. and Horikoshi,K.
TITLE Nucleotide sequences of two cellulase genes from alkalophilic
Bacillus sp. strain N-4 and their strong homology
JOURNAL J. Bacteriol. 168, 479-485 (1986)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Nakamura,A., Fukumori,F., Horinouchi,S., Masaki,H., Kudo,T.,
Uozumi,T., Horikoshi,K. and Beppu,T.
TITLE Construction and characterization of the chimeric enzymes between
the bacillus subtilis cellulase and an alkalophilic bacillus
cellulase
JOURNAL J. Biol. Chem. 266, 1579-1583 (1991)
STANDARD full staff_review
COMMENT A region of dyad symmetry, which could be a transcription
termination signal, is located at positions 1665-1697.
FEATURES Location/Qualifiers
CDS 192..1658
/note="cellulase (EC 3.2.1.4)"
/codon_start=192
repeat_region 1212..1391
/note="repeat copy A"
repeat_region 1476..1652
/note="repeat copy B"
BASE COUNT 694 a 381 c 448 g 565 t
ORIGIN 1 bp upstream of HindIII site.
1 aagcttattt tattgattag gtatattcac tgattgaata aagagagttt gttttataaa
61 attaacaacg aaaaaaccat tttcgttcgg gaggagttga tagcgcttac aattcgctag
121 tttataagtg tgtttagaaa ctaaaacgat ttcgtctcta aagtctgtaa aaaaaatttt
181 aggaggaaaa gatgaaaaaa ctaacgacta tttttattgt atttacgtta gcattattat
241 ttgttggaaa ctctacttct gcaaataatg gttccgttgt tgagcaaaat ggacaattaa
301 gtattcaaaa cggtcaatta gtgaatgaac atggagaccc ggtacagcta aagggaatga
361 gttcacatgg attacaatgg tatggacaat ttgtaaacta cgatagtata aagtggttaa
421 gagacgattg gggaataacc gtattccgag cagcgatgta tacatcttca ggagggtata
481 ttgaagatcc ttcagtaaaa gaaaaagtaa aagaggctgt tgaagctgcg attgatctcg
541 gaatatatgt cattattgac tggcatattc tttctgataa tgacccgaac atttataagg
601 aagaagcaaa agagtttttt gatgaaatgt ctgcacttta tggggattat ccgaatgtca
661 tttatgagat tgcgaatgaa ccaaacggac ataatgtaag atgggatagc catattaaac
721 catatgcaga ggaagtaatc ccagtaatcc gtgcaaacga tcctaataat attgtcattg
781 ttgggacagc aacttggagt caggatgtac acgaagcagc tgataaccaa ctagatgacc
841 cgaatgtaat gtatgcgttc cacttctacg ctggaacaca tggtcagcaa ttaagaaatc
901 aagttgacta tgctttaagt cgaggagcag cgatattcgt tagtgagtgg ggaacgagtg
961 cggctacggg tgatggtggc gtatttttag atgaagccca agtgtggatt gactttatgg
1021 atgagagaaa tttaagctgg gcaaactggt ctttaacaca taaagatgag tcatctgcag
1081 cgttaatgcc tggtgcaaac ccaactggtg ggtggacagc ggctgaattg tctccatctg
1141 gtgcatttgt gagggaaaaa ataagagaat cagcgtctat tccgccaagc gatccaacac
1201 ctccatctga tccagatcca ggtgagccgg acccaacgcc accaagtgat ccaggggagt
1261 atccagcatg ggatccaaat caaatttaca caaatgaaat tgtgtatcat aacggtcagt
1321 tatggcaagc gaaatggtgg acacaaaatc aagagccggg agctaatcaa tatgggccat
1381 gggagccttt aggagatgct cctccaagtg aaccgagcga tccaccacca ccatcagaac
1441 cagagccgga cccaggagaa ccagatccag gtgagccaga cccaggagaa ccggatccaa
1501 cgccaccaag tgatccagga gagtatccag catgggaccc aacgcaaatt tacacgaatg
1561 aaattgttta ccataacgga caattgtggc aagcaaaatg gtggacacaa aatcaagaac
1621 ccggatatcc ttatggacca tgggagccgt taaactaaca tattaatcat aaatgaaaag
1681 gtgctcagca aagaggagca ccttttctaa caaaacttta gtccttcgct ctaacaagag
1741 gcatagtaca acatctaaaa gtaccaccgg acttaataat ttctgtaata tcgacttcta
1801 tcacttcata accacgaata cgaagctgct gattgacttc tctatttata ggtaagctaa
1861 caattctttt tttaccaata gaaagtacgt ttgttccaag tgtgaactgc tcttcttcat
1921 ttacttcaat taaatcaaaa tgctttgcta atatcttttc ttcctttttg tcaaaagctc
1981 gtgggaaata taacgcttca ttctctgaaa taatatgaac acacaatcta agtgtaaata
2041 tttttcagta aaaggtacgg ttattacttc gtactcgtgt aaaagctt
//