GenBank-Updates@genbank.bio.net (04/26/91)
LOCUS ECOCYSAA 5280 bp ds-DNA BCT 26-APR-1991
DEFINITION E.coli nirC and open reading frames 3 and 4, complete cds and cysG
gene, 3' end.
ACCESSION M64606
KEYWORDS .
SOURCE E.coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 5280)
AUTHORS Wu,J.-Y., Siegel,L.M. and Kredich,N.M.
TITLE High-level expression of escherichia coli nadph-sulfite reductase:
requirement for a cloned cysG plasmid to overcome limiting siroheme
cofactor
JOURNAL J. Bacteriol. 173, 325-333 (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 469..1278
/gene="nirC"
/codon_start=469
CDS 1290..2663
/gene="cysG"
/codon_start=1290
/partial
CDS 2997..4247
/note="open reading frame 3"
/codon_start=2997
CDS 4433..5227
/note="open reading frame 4"
/codon_start=4433
BASE COUNT 1226 a 1458 c 1467 g 1129 t
ORIGIN
1 gatcagcaac atcgacccgt tctttgaggc cagtgtgctg tctcgtgggc tgattgcgga
61 acaccagggc gaactgtggg tcgccagccc gttgaaaaag cagccgttcc gcctgagcga
121 tggtttatgc atggaagatg agcagttttc tgtgaaacat tacgacgcgc gggtaaaaga
181 cggcgtggtg cagttgcgcg gttaattatt tttggaggcg caacgcctcc ccttttttgt
241 attttttttc attttaattg ttatcacaaa atcatttgta ctgcatcgcg gcggcaactg
301 agcaacaaat tcgtcaggaa cgaatttgaa cagccgctgg ctgccttcgg tgagggacaa
361 ggatgtccct cattaatccc caggagatag ttcactatgt gactggggtg agcgagggca
421 gccaacaaag aggcagtgca aaggatgaag tgagaaaagg ataatcaaat gtttacagac
481 tctatcaata agtgtgcggc taagctgcgc gcatctgcac ccgtgtcggc gaataatccg
541 ctcggcttct gggtcagttc ggcaatggcc ggggcttatg tcggtctcgg catcattctt
601 attttcaccc tcggcaatct gctcgacccg tccgtacgtc ctctggtgat gggagccacc
661 ttcggtatcg ccttaacgct ggtcatcatc gccggttccg aactgtttac cggccacacc
721 atgttcctga cgctgggcgt caaagcaggc accatcagcc acggtcaaat gtgggctatc
781 ctgccgcaaa cctggctcgg caacctggtc ggttccgtgt ttgtcgccct gctttacagc
841 tggggcggcg gcagtttatt gccggtcgat accagcatcg ttcactcagt cgcgctggcg
901 aaaaccaccg cgcccgccac ggtactgttc ttcaaaggcg cgctgtgtaa ctggctggtt
961 tgtctggcaa tctggatggc aatccgcacc gaaggcacgg caaaatttct tgctatctgg
1021 tggtgtctgc tggcgtttat cgcttccggc tacgagcact ccgtcgcgaa tatgacgctg
1081 ttcgccctct cctggtttgg tcatcacagc gacgcctata ccctggccgg aattggtcat
1141 aacctgttgt gggtgacact cggtaatact ttgtccggtg tcgtattcat gggattgggt
1201 tattggtatg ctacgccaaa atcggagcgt ccggctccgg caaaaatcaa tcagccagag
1261 gctgctgcca ataattaagg ggtaatgtcg tggaccattt gcctatattt tgtcaattac
1321 gcgaccgcga ctgtctgatc gtcggcggtg gcgatgtcgc agaacgcaaa gcacggttac
1381 tgctggaagc aggcgcacgt ttaacggtca atgcgctaac ctttattcca cagttcaccg
1441 tatgggcaaa tgaaggcatg ttgactctgg ttgagggacc gttcgacgaa acgcttctcg
1501 actcgtgttg gctggcgatc gcggccactg acgacgatac cgtcaaccag cgcgtcagcg
1561 acgcggcgga gtcacgccgt atcttttgca acgtggtgga tgcgccgaaa gccgccagct
1621 ttatcatgcc ctccattatt gaccgctcgc cgctgatggt cgccgtctcc tcgggcggca
1681 cctccccggt gctggcgcgt ctgctgcgcg agaaactgga atcgctgctg ccgcagcatc
1741 tggggcaggt cgcgcgctat gccgggcaac tccgcgcccg agtgaaaaag cagtttgcca
1801 cgatgggcga gcgtcgtcgc ttctgggaaa aatttttcgt caatgaccgg ctggcgcagt
1861 cgctggcgaa tgccgatgag aaagcggtta acgcgacaac cgaacgcctg tttagcgaac
1921 cgctggatca ccgtggcgaa gtcgtgctgg tcggcgccgg gccgggcgat gccggactgc
1981 tgacgctgaa agggttacaa caaatccaac aggcggatat cgtggtttac gatcgcctcg
2041 tctccgacga cattatgaac ctggtacgcc gcgatgccga tcgggtcttt gtggggaaac
2101 gcgcgggtta ccactgcgtc ccacaggagg aaatcaacca gatcctgctg cgtgaagcgc
2161 aaaaaggtaa acgcgtggta cgcctgaaag gcggcgatcc ctttatcttt ggtcgcggcg
2221 gcgaagagct ggaaacgctg tgtcatgccg gtattccttt ctcggtagtg ccggggatta
2281 ccgcggcttc cggctgctcc gcctactccg gtattccgct aactcatcgc gattacgccc
2341 agagcgtacg tctggtcacg ggtcacctga aaaccggcgg cgagctggac tgggaaaacc
2401 tggcggcaga aaaacagacg ctggtgttct acatggggct gaatcaggca gcgactatcc
2461 aggaaaaact gatcgcattc ggtatgcagg ccgatatgcc ggttgcgctg gtagaaaacg
2521 gtacctccgt gaagcaacgc gtcgtccacg gtgtgctgac gcagctcggt gaattagcgc
2581 aacaggttga aagcccggcg ctgattatcg ttggtcgcgt ggtagcctta cgcgataaat
2641 taaattggtt ctctaatcat taattaaaat aacgccctgt tatcagggct ttattttaca
2701 actactcgta atctcaaatt atttttactt aaaagtgaat taagaaacta actttaaata
2761 caccggacaa atttaaataa taattctgcc taaaaaccct tttactcgtc aaattcacct
2821 cttattcatt catacaataa ataacaccgt taagcactca atttgacctg acctggttat
2881 cgggtgataa aataaacact aaagcataat ttttcttctg gccatttcat cattgcctgt
2941 accgctctct gcatatgttt agtacgcaag gaaaatatta attaaggatg aaccctatgc
3001 aaaagaaaaa acttatttct atcgctatcg ctttaacgct acaaagttat tacattccgg
3061 ccatcgccgc agaaaataac gatgatgaaa aagaatgtcc cagtaatatc tcctccctgc
3121 ctaaagaaaa acgcgcaaaa ctctcaccga cctgccttgc tacacctgaa aatgataatc
3181 actggggctg ggttgctggc ggcgttgctg cattggtcgc aggtgtggcg attggcgttg
3241 aaaataacgg tggcggagat tctaatcatt cttatacccc ccctaagccc gataatggcg
3301 gcgacgtcac cccgcccgac gatggcggca acgtcacccc gcccgacgat ggcggcaacg
3361 tcaccccgcc cgacgatggc ggcgatgaca atgtgacccc gcccgacgat agtggcgatg
3421 acgatgtggc cccgcctgac gatagcggcg atgacgatgt aaccccgccc gacgatagcg
3481 gcgatgatga tgtgaccccg cccgacgata gcggcgatgg cgatgtgacc ccgcccgacg
3541 atagcggcga tgacgatgta accccgcccg acgatagcgg cgatgacgat gtgaccccgc
3601 ctgacgatag cggcgatgac gatgtaaccc cgcccgacga tagcggcgat gacgatgtga
3661 ccccgcccga tgatagcggc gatgacgatg taaccccgcc cgatgatagc ggcgatgacg
3721 acgacacgcc cccagatgac tctgttatta ccttcagcaa cggcgtcacc atcgataaag
3781 gcaaagacac cctgaccttc gacagcttca aactggataa cggcagcgtt cttgagggtg
3841 ccgtgtggaa ttattcagaa caggacaacc agtggcagct caccaccgcg gacggtaaaa
3901 cgctgaacgt caccggctgg gacgtgaccg acgccaatgc cgccgtgatt gaaggcaccc
3961 aggaaaacgg tctctactgg aagtacgaca gccggggcta tctgattatt gccgacgata
4021 acaccaccgt tatcagcggc gatgaccagg cgcataattc cgatcgcggc atggatatca
4081 gcggccagga tcgcaccggc gtgattattt ccggcgatag aaccgtcaac acgctcaccg
4141 gggactccag tgtgaccgac ggtgccaccg gcatggttat ctccggtgac ggcaccacca
4201 acaccatttc gggccactcc acggtggaca agccaccggc gcgctgattt ccggcaacgg
4261 caccaccacc aatttcgccg gtgacattgc cgtgagcggc ggcggcaccg ccatcatcat
4321 cgacggcgac aacgccacga ttaagaatac cggtacctct gacatcagcg gcgcaggctc
4381 caccggcacc gtcattgacg gcaataacgc ccgcgtcaac aatgacggtg atatgaccat
4441 caccgacggc ggcaccggcg gccacattac cggcgacaac gtggttatcg ataacgccgg
4501 gagcactacc gtcagcggcg cagaccgcac ggcgctgtat atcgaaggcg acaacgcgct
4561 cgttatcaac gaaggtaatc aaactatctc tggcggcgcc gtcggtacgc gcattgacgg
4621 cgacgacgcc cataccacca ataccggtga tatcgcggtg gatggcgcgg gctctgccgc
4681 cgtgattatc aacggcgaca acggcagcct gacccaggcg ggcgatctgc tggtcaccga
4741 cggcgcgatg ggcatcatca cctatggcac cggaaatgaa gcaaaaaata ccggcaacgc
4801 caccgtacgt gatgcggact cggtgggttt tgtggttgca ggcgaaaaaa acaccttcaa
4861 aaacaaaggg gatattgacg tcagccttaa cggcaccggc gcgctggtga gcggcgatat
4921 gtcgcaggtt acgctggatg gcgatattaa cgttgtctca gtccaggaca gcgaaggcgt
4981 gtttagctca gcgacagggg tgagcgtgag cggcgacagc aacgccgttg atatcaccgg
5041 caacgtaaat atcagcgccg actacgggca ggatgatctg gctgccgggg ctcccccgtt
5101 aaccggcgtt gtcgtcggcg gtaacggcaa taccgttacc cttaatggcg cgctgaatat
5161 tgatgacaac gatctgtcgc accggcggac aatacctgga cgttgttggc ctgagcgtaa
5221 caggtgatga taacgacgtt gagattgacg gcggtattaa tatcacccac agcgaggatc
//