GenBank-Updates@genbank.bio.net (05/01/91)
LOCUS PHVCPTRL1 88 bp ss-tRNA RNA 01-MAY-1991
DEFINITION P.vulgaris (bean) chloroplast Leu-tRNA-1.
ACCESSION K00235 M58294
KEYWORDS transfer RNA; transfer RNA-Leu.
SOURCE Phaseolus vulgaris (bean) leaf chloroplast tRNA.
ORGANISM Phaseolus vulgaris
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Rosidae; Rosales; Fabaceaea.
REFERENCE 1 (bases 1 to 88)
AUTHORS Osorio,M.L., Guillemaut,P., Keith,G., Canaday,J. and Weil,J.-H.
TITLE Primary structure of three leucine transfer RNAs from bean
chloroplast
JOURNAL Biochem. Biophys. Res. Commun. 92, 102-108 (1980)
STANDARD simple staff_review
REFERENCE 2 (sites)
AUTHORS Burkard,G., Canaday,J., Crouse,E.J., Gloeckler,R., Gordon,K.,
Guillemaut,P., Keith,G., Kuntz,M., Mubumbila,M., Osorio,M.L.,
Steinmetz,A. and Weil,J.H.
TITLE Protein synthesis in chloroplasts: Sequence studies and gene
mapping studies on chloroplast transfer RNAs
JOURNAL Cienc. Biol. 7, 27-37 (1982)
STANDARD full staff_review
COMMENT Contributed on tape April 1983 by M.Sprinzl & D.H.Gauss; from their
entry 1075 in Nucleic Acids Res. 11, r1-r54 (1983). [Biochem.
Biophys. Res. Commun. 92, 102-108 (1980)] also
sequenced P.vulgaris cp Leu-tRNA-2 and Leu-tRNA-3. [Biochem.
Biophys. Res. Commun. 92, 102-108 (1980)] uncertain
about identity of modified base at 38.
FEATURES Location/Qualifiers
tRNA 1..88
/anticodon=(pos:35..37,aa:Leu)
/note="Leu-tRNA-1 (NAR: 1075)"
modified_base 18..18
/note="gm = 2'-o-methylguanosine"
modified_base 20..20
/note="d = dihydrouridine"
modified_base 27..27
/note="f = pseudouridine"
modified_base 35..35
/note="unidentified uridine derivative"
modified_base 38..38
/note="i6a = n6-isopentenyladenosine, or zeatin (io6a)"
modified_base 40..40
/note="f = pseudouridine"
modified_base 66..66
/note="t = 5-methyluridine"
modified_base 67..67
/note="f = pseudouridine"
BASE COUNT 26 a 21 c 20 g 21 t
ORIGIN 5' end of mature tRNA.
1 ggggatatgg cgaaattggt agacgctacg gacttaaaat ccgtcgactt aataaatcat
61 gagggttcaa gtccctctat ccccacca
//GenBank-Updates@genbank.bio.net (05/01/91)
LOCUS PHVCPTRL2 85 bp ss-tRNA RNA 01-MAY-1991
DEFINITION P.vulgaris (bean) chloroplast Leu-tRNA-2.
ACCESSION K00236 M58296
KEYWORDS transfer RNA; transfer RNA-Leu.
SOURCE Phaseolus vulgaris (bean) leaf chloroplast tRNA.
ORGANISM Phaseolus vulgaris
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Rosidae; Rosales; Fabaceaea.
REFERENCE 1 (bases 1 to 85)
AUTHORS Osorio,M.L., Guillemaut,P., Keith,G., Canaday,J. and Weil,J.-H.
TITLE Primary structure of three leucine transfer RNAs from bean
chloroplast
JOURNAL Biochem. Biophys. Res. Commun. 92, 102-108 (1980)
STANDARD simple staff_review
REFERENCE 2 (sites)
AUTHORS Burkard,G., Canaday,J., Crouse,E.J., Gloeckler,R., Gordon,K.,
Guillemaut,P., Keith,G., Kuntz,M., Mubumbila,M., Osorio,M.L.,
Steinmetz,A. and Weil,J.H.
TITLE Protein synthesis in chloroplasts: Sequence studies and gene
mapping studies on chloroplast transfer RNAs
JOURNAL Cienc. Biol. 7, 27-37 (1982)
STANDARD full staff_review
COMMENT Contributed on tape April 1983 by M.Sprinzl & D.H.Gauss; from their
entry 1076 in Nucleic Acids Res. 11, r1-r54 (1983). [Biochem.
Biophys. Res. Commun. 92, 102-108 (1980)] also
sequenced P.vulgaris cp Leu-tRNA-1 and Leu-tRNA-3 [Biochem.
Biophys. Res. Commun. 92, 102-108 (1980)] suggests
that modified base at 38 is one of the following: i6a, ms2i6a, io6a
or ms2io6a.
FEATURES Location/Qualifiers
tRNA 1..85
/anticodon=(pos:35..37,aa:Leu)
/note="Leu-tRNA-2 (NAR: 1076)"
modified_base 18..18
/note="gm = 2'-o-methylguanosine"
modified_base 20..20
/note="d = dihydrouridine"
modified_base 35..35
/note="cm = 2'-o-methylcytidine"
modified_base 38..38
/note="unidentified adenosine derivative"
modified_base 63..63
/note="t = 5-methyluridine"
modified_base 64..64
/note="f = pseudouridine"
BASE COUNT 23 a 18 c 24 g 20 t
ORIGIN 5' end of mature tRNA.
1 ggcttgatgg tgaaattggt agacacgcga gactcaaaat ctcgtgctaa agagcgtgga
61 ggttcgagtc ctcttcaagt cacca
//GenBank-Updates@genbank.bio.net (05/01/91)
LOCUS PHVCPTRL3 83 bp ss-tRNA RNA 01-MAY-1991
DEFINITION P.vulgaris (bean) chloroplast Leu-tRNA-3.
ACCESSION K00237 M58298
KEYWORDS transfer RNA; transfer RNA-Leu.
SOURCE Phaseolus vulgaris (bean) leaf chloroplast tRNA.
ORGANISM Phaseolus vulgaris
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Rosidae; Rosales; Fabaceaea.
REFERENCE 1 (bases 1 to 83)
AUTHORS Osorio,M.L., Guillemaut,P., Keith,G., Canaday,J. and Weil,J.-H.
TITLE Primary structure of three leucine transfer RNAs from bean
chloroplast
JOURNAL Biochem. Biophys. Res. Commun. 92, 102-108 (1980)
STANDARD simple staff_review
REFERENCE 2 (sites)
AUTHORS Burkard,G., Canaday,J., Crouse,E.J., Gloeckler,R., Gordon,K.,
Guillemaut,P., Keith,G., Kuntz,M., Mubumbila,M., Osorio,M.L.,
Steinmetz,A. and Weil,J.H.
TITLE Protein synthesis in chloroplasts: Sequence studies and gene
mapping studies on chloroplast transfer RNAs
JOURNAL Cienc. Biol. 7, 27-37 (1982)
STANDARD full staff_review
COMMENT Contributed on tape April 1983 by M.Sprinzl & D.H.Gauss; from their
entry 1077 in Nucleic Acids Res. 11, r1-r54 (1983). [Biochem.
Biophys. Res. Commun. 92, 102-108 (1980)] also
sequenced P.vulgaris cp Leu-tRNA-1 and Leu-tRNA-2.
FEATURES Location/Qualifiers
tRNA 1..83
/anticodon=(pos:35..37,aa:Leu)
/note="Leu-tRNA-3 (NAR: 1077)"
modified_base 18..18
/note="gm = 2'-o-methylguanosine"
modified_base 20..20
/note="d = dihydrouridine"
modified_base 37..37
/note="m7g = 7-methylguanosine"
modified_base 38..38
/note="m1g = 1-methylguanosine"
modified_base 61..61
/note="t = 5-methyluridine"
modified_base 62..62
/note="f = pseudouridine"
BASE COUNT 19 a 20 c 26 g 18 t
ORIGIN 5' end of mature tRNA.
1 gccgctatgg tgaaattggt agacacgctg ctcttaggaa gcagtgctag agcatctcgg
61 ttcgagtccg agtagcggca cca
//