GenBank-Updates@genbank.bio.net (05/01/91)
LOCUS PHVCPTRL1 88 bp ss-tRNA RNA 01-MAY-1991 DEFINITION P.vulgaris (bean) chloroplast Leu-tRNA-1. ACCESSION K00235 M58294 KEYWORDS transfer RNA; transfer RNA-Leu. SOURCE Phaseolus vulgaris (bean) leaf chloroplast tRNA. ORGANISM Phaseolus vulgaris Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida; Rosidae; Rosales; Fabaceaea. REFERENCE 1 (bases 1 to 88) AUTHORS Osorio,M.L., Guillemaut,P., Keith,G., Canaday,J. and Weil,J.-H. TITLE Primary structure of three leucine transfer RNAs from bean chloroplast JOURNAL Biochem. Biophys. Res. Commun. 92, 102-108 (1980) STANDARD simple staff_review REFERENCE 2 (sites) AUTHORS Burkard,G., Canaday,J., Crouse,E.J., Gloeckler,R., Gordon,K., Guillemaut,P., Keith,G., Kuntz,M., Mubumbila,M., Osorio,M.L., Steinmetz,A. and Weil,J.H. TITLE Protein synthesis in chloroplasts: Sequence studies and gene mapping studies on chloroplast transfer RNAs JOURNAL Cienc. Biol. 7, 27-37 (1982) STANDARD full staff_review COMMENT Contributed on tape April 1983 by M.Sprinzl & D.H.Gauss; from their entry 1075 in Nucleic Acids Res. 11, r1-r54 (1983). [Biochem. Biophys. Res. Commun. 92, 102-108 (1980)] also sequenced P.vulgaris cp Leu-tRNA-2 and Leu-tRNA-3. [Biochem. Biophys. Res. Commun. 92, 102-108 (1980)] uncertain about identity of modified base at 38. FEATURES Location/Qualifiers tRNA 1..88 /anticodon=(pos:35..37,aa:Leu) /note="Leu-tRNA-1 (NAR: 1075)" modified_base 18..18 /note="gm = 2'-o-methylguanosine" modified_base 20..20 /note="d = dihydrouridine" modified_base 27..27 /note="f = pseudouridine" modified_base 35..35 /note="unidentified uridine derivative" modified_base 38..38 /note="i6a = n6-isopentenyladenosine, or zeatin (io6a)" modified_base 40..40 /note="f = pseudouridine" modified_base 66..66 /note="t = 5-methyluridine" modified_base 67..67 /note="f = pseudouridine" BASE COUNT 26 a 21 c 20 g 21 t ORIGIN 5' end of mature tRNA. 1 ggggatatgg cgaaattggt agacgctacg gacttaaaat ccgtcgactt aataaatcat 61 gagggttcaa gtccctctat ccccacca //
GenBank-Updates@genbank.bio.net (05/01/91)
LOCUS PHVCPTRL2 85 bp ss-tRNA RNA 01-MAY-1991 DEFINITION P.vulgaris (bean) chloroplast Leu-tRNA-2. ACCESSION K00236 M58296 KEYWORDS transfer RNA; transfer RNA-Leu. SOURCE Phaseolus vulgaris (bean) leaf chloroplast tRNA. ORGANISM Phaseolus vulgaris Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida; Rosidae; Rosales; Fabaceaea. REFERENCE 1 (bases 1 to 85) AUTHORS Osorio,M.L., Guillemaut,P., Keith,G., Canaday,J. and Weil,J.-H. TITLE Primary structure of three leucine transfer RNAs from bean chloroplast JOURNAL Biochem. Biophys. Res. Commun. 92, 102-108 (1980) STANDARD simple staff_review REFERENCE 2 (sites) AUTHORS Burkard,G., Canaday,J., Crouse,E.J., Gloeckler,R., Gordon,K., Guillemaut,P., Keith,G., Kuntz,M., Mubumbila,M., Osorio,M.L., Steinmetz,A. and Weil,J.H. TITLE Protein synthesis in chloroplasts: Sequence studies and gene mapping studies on chloroplast transfer RNAs JOURNAL Cienc. Biol. 7, 27-37 (1982) STANDARD full staff_review COMMENT Contributed on tape April 1983 by M.Sprinzl & D.H.Gauss; from their entry 1076 in Nucleic Acids Res. 11, r1-r54 (1983). [Biochem. Biophys. Res. Commun. 92, 102-108 (1980)] also sequenced P.vulgaris cp Leu-tRNA-1 and Leu-tRNA-3 [Biochem. Biophys. Res. Commun. 92, 102-108 (1980)] suggests that modified base at 38 is one of the following: i6a, ms2i6a, io6a or ms2io6a. FEATURES Location/Qualifiers tRNA 1..85 /anticodon=(pos:35..37,aa:Leu) /note="Leu-tRNA-2 (NAR: 1076)" modified_base 18..18 /note="gm = 2'-o-methylguanosine" modified_base 20..20 /note="d = dihydrouridine" modified_base 35..35 /note="cm = 2'-o-methylcytidine" modified_base 38..38 /note="unidentified adenosine derivative" modified_base 63..63 /note="t = 5-methyluridine" modified_base 64..64 /note="f = pseudouridine" BASE COUNT 23 a 18 c 24 g 20 t ORIGIN 5' end of mature tRNA. 1 ggcttgatgg tgaaattggt agacacgcga gactcaaaat ctcgtgctaa agagcgtgga 61 ggttcgagtc ctcttcaagt cacca //
GenBank-Updates@genbank.bio.net (05/01/91)
LOCUS PHVCPTRL3 83 bp ss-tRNA RNA 01-MAY-1991 DEFINITION P.vulgaris (bean) chloroplast Leu-tRNA-3. ACCESSION K00237 M58298 KEYWORDS transfer RNA; transfer RNA-Leu. SOURCE Phaseolus vulgaris (bean) leaf chloroplast tRNA. ORGANISM Phaseolus vulgaris Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida; Rosidae; Rosales; Fabaceaea. REFERENCE 1 (bases 1 to 83) AUTHORS Osorio,M.L., Guillemaut,P., Keith,G., Canaday,J. and Weil,J.-H. TITLE Primary structure of three leucine transfer RNAs from bean chloroplast JOURNAL Biochem. Biophys. Res. Commun. 92, 102-108 (1980) STANDARD simple staff_review REFERENCE 2 (sites) AUTHORS Burkard,G., Canaday,J., Crouse,E.J., Gloeckler,R., Gordon,K., Guillemaut,P., Keith,G., Kuntz,M., Mubumbila,M., Osorio,M.L., Steinmetz,A. and Weil,J.H. TITLE Protein synthesis in chloroplasts: Sequence studies and gene mapping studies on chloroplast transfer RNAs JOURNAL Cienc. Biol. 7, 27-37 (1982) STANDARD full staff_review COMMENT Contributed on tape April 1983 by M.Sprinzl & D.H.Gauss; from their entry 1077 in Nucleic Acids Res. 11, r1-r54 (1983). [Biochem. Biophys. Res. Commun. 92, 102-108 (1980)] also sequenced P.vulgaris cp Leu-tRNA-1 and Leu-tRNA-2. FEATURES Location/Qualifiers tRNA 1..83 /anticodon=(pos:35..37,aa:Leu) /note="Leu-tRNA-3 (NAR: 1077)" modified_base 18..18 /note="gm = 2'-o-methylguanosine" modified_base 20..20 /note="d = dihydrouridine" modified_base 37..37 /note="m7g = 7-methylguanosine" modified_base 38..38 /note="m1g = 1-methylguanosine" modified_base 61..61 /note="t = 5-methyluridine" modified_base 62..62 /note="f = pseudouridine" BASE COUNT 19 a 20 c 26 g 18 t ORIGIN 5' end of mature tRNA. 1 gccgctatgg tgaaattggt agacacgctg ctcttaggaa gcagtgctag agcatctcgg 61 ttcgagtccg agtagcggca cca //