GenBank-Updates@genbank.bio.net (05/01/91)
LOCUS PHVCPTRMF 77 bp ss-tRNA RNA 01-MAY-1991
DEFINITION Phaseolus vulgaris (bean) chloroplast initiator Met-tRNA-f.
ACCESSION K00319 M58300
KEYWORDS transfer RNA; transfer RNA-Met.
SOURCE Phaseolus vulgaris (bean) chloroplast tRNA.
ORGANISM Phaseolus vulgaris
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Rosidae; Rosales; Fabaceaea.
REFERENCE 1 (bases 1 to 77)
AUTHORS Canaday,J., Guillemaut,P. and Weil,J.-H.
TITLE The nucleotide sequences of the initiator transfer RNAs from bean
cytoplasm and chloroplasts
JOURNAL Nucleic Acids Res. 8, 999-1008 (1980)
STANDARD simple staff_review
REFERENCE 2 (sites)
AUTHORS Burkard,G., Canaday,J., Crouse,E.J., Gloeckler,R., Gordon,K.,
Guillemaut,P., Keith,G., Kuntz,M., Mubumbila,M., Osorio,M.L.,
Steinmetz,A. and Weil,J.H.
TITLE Protein synthesis in chloroplasts: Sequence studies and gene
mapping studies on chloroplast transfer RNAs
JOURNAL Cienc. Biol. 7, 27-37 (1982)
STANDARD full staff_review
COMMENT Contributed on tape April 1983 by M.Sprinzl & D.H.Gauss; from their
entry 1377 in Nucleic Acids Res. 11, r1-r54 (1983). Also sequenced
is the bean Met-tRNA-i. This sequence resembles prokaryotic
initiator tRNAs.
FEATURES Location/Qualifiers
tRNA 1..77
/anticodon=(pos:35..37,aa:Met)
/note="Met-tRNA-f (NAR: 1377)"
modified_base 19..19
/note="gm = 2'-o-methylguanosine"
modified_base 21..21
/note="d = dihydrouridine"
modified_base 47..47
/note="m7g = 7-methylguanosine"
modified_base 48..48
/note="x = 3-(3-amino-3-carboxypropyl)uridine, (acp3)u"
modified_base 55..55
/note="t = 5-methyluridine"
modified_base 56..56
/note="f = pseudouridine"
BASE COUNT 20 a 22 c 19 g 16 t
ORIGIN 5' end of mature tRNA.
1 cgcggagtag agcaacttgg tagctcgcaa ggctcataac cttgaagtta cgggttcaaa
61 tcccgtctcc gcaacca
//