GenBank-Updates@genbank.bio.net (05/01/91)
LOCUS PHVTRMI 75 bp ss-tRNA RNA 01-MAY-1991
DEFINITION Phaseolus vulgaris initiator Met-tRNA-i.
ACCESSION K00318 M58302
KEYWORDS transfer RNA; transfer RNA-Met.
SOURCE Phaseolus vulgaris (bean) leaves tRNA.
ORGANISM Phaseolus vulgaris
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Rosidae; Rosales; Fabaceaea.
REFERENCE 1 (bases 1 to 75)
AUTHORS Canaday,J., Guillemaut,P. and Weil,J.-H.
TITLE The nucleotide sequences of the initiator transfer RNAs from bean
cytoplasm and chloroplasts
JOURNAL Nucleic Acids Res. 8, 999-1008 (1980)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Burkard,G., Canaday,J., Crouse,E.J., Gloeckler,R., Gordon,K.,
Guillemaut,P., Keith,G., Kuntz,M., Mubumbila,M., Osorio,M.L.,
Steinmetz,A. and Weil,J.H.
TITLE Protein synthesis in chloroplasts: Sequence studies and gene
mapping studies on chloroplast transfer RNAs
JOURNAL Cienc. Biol. 7, 27-37 (1982)
STANDARD full staff_review
COMMENT Contributed on tape April 1983 by M.Sprinzl & D.H.Gauss; from their
entry 1376 in Nucleic Acids Res. 11, r1-r54 (1983). Also sequenced
is the bean chloroplast Met-tRNA-f. This sequence shows 73-100%
homology with eukaryotic initiator tRNAs. It is almost identical
with wheat germ Met-tRNA-i.
FEATURES Location/Qualifiers
tRNA 1..75
/anticodon=(pos:33..35,aa:Met)
/note="Met-tRNA-i (NAR: 1376)"
modified_base 9..9
/note="m1g = 1-methylguanosine"
modified_base 10..10
/note="m2g = 2-methylguanosine"
modified_base 25..25
/note="m2g = 2-methylguanosine"
modified_base 36..36
/note="t6a = n-((9-beta-d-ribofuranosylpurine-6-yl)
carbamoyl)threonine"
modified_base 45..45
/note="m7g = 7-methylguanosine"
modified_base 46..46
/note="d = dihydrouridine"
modified_base 47..47
/note="m5c = 5-methylcytidine"
modified_base 54..54
/note="f = pseudouridine"
modified_base 57..57
/note="m1a = 1-methyladenosine"
modified_base 63..63
/note="gm = 2'-o-methylguanosine"
BASE COUNT 19 a 22 c 23 g 11 t
ORIGIN 5' end of mature tRNA.
1 atcagagtgg cgcagcggaa gcgtggtggg cccataaccc acaggtccca ggatcgaaac
61 ctggctctga tacca
//