GenBank-Updates@genbank.bio.net (05/01/91)
LOCUS SPICPTRW 77 bp ss-tRNA RNA 01-MAY-1991
DEFINITION Spinach (S.oleracea) chloroplast Trp-tRNA.
ACCESSION K00262 M58304
KEYWORDS transfer RNA; transfer RNA-Trp.
SOURCE Spinach (Spinacia oleracea) leaf chloroplast tRNA.
ORGANISM Spinacia oleracea
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Caryophyllidae; Caryophyllales; Chenopodiaceae.
REFERENCE 1 (bases 1 to 77)
AUTHORS Canaday,J., Guillemaut,P., Gloeckler,R. and Weil,J.-H.
TITLE The nucleotide sequence of spinach chloroplast tryptophan transfer
RNA
JOURNAL Nucleic Acids Res. 9, 47-53 (1981)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Burkard,G., Canaday,J., Crouse,E.J., Gloeckler,R., Gordon,K.,
Guillemaut,P., Keith,G., Kuntz,M., Mubumbila,M., Osorio,M.L.,
Steinmetz,A. and Weil,J.H.
TITLE Protein synthesis in chloroplasts: Sequence studies and gene
mapping studies on chloroplast transfer RNAs
JOURNAL Cienc. Biol. 7, 27-37 (1982)
STANDARD full staff_review
COMMENT Contributed on tape April 1983 by M.Sprinzl & D.H.Gauss; from their
entry 1846 in Nucleic Acids Res. 11, r1-r54 (1983). Spinach cp
Trp-tRNA shows 68% homology with E.coli suppressor tRNA su+uga and
contains the same base pair at position 11:24 [Nucleic Acids Res.
9, 47-53 (1981)].
FEATURES Location/Qualifiers
tRNA 1..77
/anticodon=(pos:35..37,aa:Trp)
/note="Trp-tRNA (NAR: 1846)"
modified_base 19..19
/note="gm = 2'-o-methylguanosine"
modified_base 21..21
/note="d = dihydrouridine"
modified_base 27..27
/note="m2g = 2-methylguanosine"
modified_base 28..28
/note="f = pseudouridine"
modified_base 32..32
/note="f = pseudouridine"
modified_base 38..38
/note="i6a = n6-isopentenyladenosine (or ms2i6a =
2-methylthio-n6-isopentenyladenosine"
modified_base 48..48
/note="unidentified modified nucleotide"
modified_base 55..55
/note="t = 5-methyluridine"
modified_base 56..56
/note="f = pseudouridine"
BASE COUNT 17 a 20 c 21 g 18 t 1 others
ORIGIN 5' end of mature tRNA.
1 gcgctcttag ttcagttcgg tagaacgtgg gtctccaaaa cccgatgncg taggttcaag
61 tcctacagag cgtgcca
//