GenBank-Updates@genbank.bio.net (05/01/91)
LOCUS SHDTOXAB 1320 bp ds-DNA BCT 01-MAY-1991
DEFINITION S.dysenteriae toxin alpha- and beta-subunit gene, complete cds.
ACCESSION X07903 M32511
KEYWORDS enterotoxin; toxin.
SOURCE S.dysenteriae DNA, clone pCH79.
ORGANISM Shigella dysenteriae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1320)
AUTHORS Kozlov,Y.V., Kabishev,A.A., Fedchenko,V.I. and Baev,A.
TITLE Cloning and primary structure of Shigella toxin genes
JOURNAL Dokl. Biochem. 295, 216-220 (1987)
STANDARD simple automatic
REFERENCE 2 (bases 1 to 1320)
AUTHORS Kozlov,Y.V., Kabishev,A.A., Fedchenko,V.I. and Baev,A.A.
TITLE Cloning and primary structure of Shigella toxin genes
JOURNAL Dokl. Biochem. 295, 740-744 (1987)
STANDARD simple automatic
COMMENT EMBL features not translated to GenBank features:
key from to description
PRM 66 72 put.-35 region
PRM 91 96 put.-10 region
RBS 112 117 put.rRNA binding site
RPT 997 1004 direct repeat 1
INVREP 1008 1014 inverted repeat A
INVREP 1018 1024 inverted repeat A'
RPT 1022 1030 direct repeat 1
PRM 1028 1033 put.-35 region
RBS 1075 1078 put.rRNA binding site
FEATURES Location/Qualifiers
CDS 129..1076
/note="toxin alpha-subunit precursor"
/codon_start=129
sig_peptide 129..194
/note="toxin alpha-subunit signal peptide"
/codon_start=129
mat_peptide 195..1073
/note="toxin alpha-subunit"
/codon_start=195
CDS 1086..>1320
/note="toxin beta-subunit precursor"
/codon_start=1086
sig_peptide 1086..1145
/note="toxin beta-subunit signal peptide"
/codon_start=1086
mat_peptide 1146..>1320
/note="toxin beta-subunit"
/codon_start=1146
BASE COUNT 359 a 223 c 316 g 422 t
ORIGIN
1 gaatatgatt atcattttca ttacgttatt gttacgttta tccggtgcgc cgtaaaacgc
61 cgtccttcag ggcgtggagg atgtcaagaa tatagttatc gtatggtgct caaggagtat
121 tgtgtaatat gaaaataatt atttttagag tgctaacttt tttctttgtt atcttttcag
181 ttaatgtggt tgcgaaggaa tttaccttag acttctcgac cgcaaagacg tatgtagatt
241 cgctgaatgt cattcgctct gcaataggta ctccattaca gactatttca tcaggaggta
301 cgtctttact gatgattgat agtggcacag gggataattt gtttgcagtt gatgtcagag
361 ggatagatcc agaggaaggg cggtttaata atctacggct tattgttgaa cgaaataatt
421 tatatgtgac aggatttgtt aacaggacaa ataatgtttt ttatcgcttt gctgattttt
481 cacatgttac ctttccaggt acaacagcgg ttacattgtc tggtgacagt agctatacca
541 cgttacagcg tgttgcaggg atcagtcgta cggggatgca gataaatcgc cattcgttga
601 ctacttctta tctggattta atgtcgcata gtggaacctc actgacgcag tctgtggcaa
661 gagcgatgtt acggtttgtt actgtgacag ctgaaccttt acgttttcgg caaatacaga
721 ggggatttcg tacaacactg gatgatctca gtgggcgttc ttatgtaatg actgctgaag
781 atgttgatct tacattgaac tggggaaggt tgagtagtgt cctgcctgac tatcatggac
841 aagactctgt tcgtgtagga agaatttctt ttggaagcat taatgcaatt ctgggaagcg
901 tggcattaat actgaattgt catcatcatg catcgcgagt tgccagaatg gcatctgatg
961 agtttccttc tatgtgtccg gcagatggaa gagtccgtgg gattacgcac aataaaatat
1021 tgtgggattc atccactctg ggggcaattc tgatgcgcag aactattagc agttgagggg
1081 gtaaaatgaa aaaaacatta ttaatagctg catcgctttc atttttttca gcaagtgcgc
1141 tggcgacgcc tgattgtgta actggaaagg tggagtatac aaaatataat gatgacgata
1201 cctttacagt taaagtgggt gataaagaat tatttaccaa cagatggaat cttcagtctc
1261 ttcttctcag tgcgcaaatt acggggatga ctgtaaccat taaaactaat gcctgtcata
//