GenBank-Updates@genbank.bio.net (05/01/91)
LOCUS PEACAB80 1993 bp ds-DNA PLN 01-MAY-1991
DEFINITION Pea (P.sativum) gene AB80 encoding major light-harvesting
chlorophyll a/b-binding protein (polypeptide 15) complex precursor,
complete coding sequence.
ACCESSION K02067
KEYWORDS chlorophyll binding protein; light-harvesting complex;
major chlorophyll binding protein; polypeptide 15.
SOURCE Pea (var. Progress 9) leaf DNA.
ORGANISM Pisum sativum
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Rosidae; Rosales; Fabaceaea.
REFERENCE 1 (bases 828 to 1967)
AUTHORS Cashmore,A.R.
TITLE Structure and expression of a pea nuclear gene encoding a
chlorophyll a/b-binding polypeptide
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 81, 2960-2964 (1984)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1993)
AUTHORS Timko,M.P., Kausch,A.P., Hand,J.M., Cashmore,A.R.,
Herrera-Estrella,L., Van den Broeck,G. and Van Montagu,M.
TITLE Structure and expression of nuclear genes encoding polypeptides of
the photosynthetic apparatus
JOURNAL (in) Steinbeck,K.E., Bonitz,S., Arntzen,C.J. and Bogorad,L. (Eds.);
Molecular Biology of the Photosynthetic Apparatus: 381-396,
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1985)
STANDARD full staff_review
COMMENT Draft entry and computer-readable copy sequence [(in) Steinbeck,
K.E., Bonitz,S., Arntzen,C.J. and Bogorad,L. (Eds.);Molecular Bio]
kindly provided
by B.Fristensky, 04-MAR-1989.
The nuclear genes encoding the major chlorophyll a/b-binding (CAB)
polypeptides are present in pea as a small multigene family. [Proc.
Natl. Acad. Sci. U.S.A. 81, 2960-2964 (1984)]
compares sequences with clone pAB96 (see separate entry). Both
encode mature chlorophyll a/b-binding polypeptides. The differences
found (7.3% divergence in the coding and 20.8% divergence in the 3'
noncoding regions) suggest that the major AB polypeptides are
closely related but distinct [Proc. Natl. Acad. Sci. U.S.A. 81,
2960-2964 (1984)].
The transit sequence of AB80 is presumed to facilitate the
translocation of the precursor polypeptide into the chloroplast.
There is some uncertainty about the NH2 terminus of the mature
protein. It could be either Met-37 (bp 292-294) or Arg-38 (bp
295-297).
FEATURES Location/Qualifiers
mRNA 943..1970
/note="polypeptide 15 mRNA"
CDS 1011..1820
/note="polypeptide 15 precursor"
/codon_start=1011
mat_peptide 1119..1817
/note="polypeptide 15"
/codon_start=1119
misc_signal 1921..1927
/note="poly-A signal"
BASE COUNT 612 a 388 c 359 g 634 t
ORIGIN 11 bp upstream of HinfI site.
1 ctgcagtttt tcaaagaaat tatcttttgt tcccttttgc attgtaactt aacaataatg
61 gcgtaaataa taataaccat gattgacttg aattttggta aacaattata attaactatt
121 ccccctccct ttcccttttt aagtgtcact tttttgataa atttttgttt tttttaagtg
181 ttccttacaa agttcaagat agtattaaat gtatctttgt caaaattacc cctatgtaat
241 tattatagag agagaaaaag taaaatgaat gtaatatata attaagggta ttataggtaa
301 aaagagaaat attatttaaa aagtaacaat aatgattaac tttcttggta tatgtaaaaa
361 gttaaaaaat taccgtttaa aaaggaacgg agggaatata tactagttta ttagatttca
421 gtaatcatgc attaaaattg accagtgcat gtgtaaccaa ccatcaacta ttatttggtt
481 cttttcacaa ttttcctacc ttacctttat cttctttatt acacttgttt cttttctcaa
541 atttcgacgc gtgttgatgt aatattggta taaacaagaa actacaaaac gcagctgcca
601 taatgtcaca atttttctca aatcttgtgg ctctcaaaca ctgtataaaa cacgacaaat
661 gtggacccaa aatatatacc ttacacttct gagttagaga agcagagccc cataattaag
721 cctattttat gaaaaaaata atattatgtt gagtcatata tccataagaa tccccacagt
781 cacacatgga agagcagcat tggatacaaa tgatatgaag attttgatcc atgaacggat
841 tctagaattg caaagaaaat ctccaactag ccatagcttt agataacaca cgataagagc
901 atctgcatta taaatacaga ctcatattca tcttacaaaa tcaccattga taaggataca
961 attatcaaaa gcataacaat cttttcaatt tcattgcaat ataatacacg atggccgcat
1021 catcatcatc atccatggct ctctcttctc caaccttggc tggcaagcaa ctcaagctga
1081 acccatcaag ccaagaattg ggagctgcaa ggttcaccat gaggaagtct gctaccacca
1141 agaaagtagc ttcctctgga agcccatggt acggaccaga ccgtgttaag tacttaggcc
1201 cattctccgg tgagtctcca tcctacttga ctggagagtt ccccggtgac tacggttggg
1261 acactgccgg actctctgct gacccagaga cattctccaa gaaccgtgag cttgaagtca
1321 tccactccag atgggctatg ttgggtgctt tgggatgtgt cttcccagag cttttgtctc
1381 gcaacggtgt taaattcggc gaagctgtgt ggttcaaggc aggatctcaa atctttagtg
1441 agggtggact tgattacttg ggcaacccaa gcttggtcca tgctcaaagc atccttgcca
1501 tatgggccac tcaggttatc ttgatgggag ctgtcgaagg ttaccgtatt gccggtgggc
1561 ctctcggtga ggtggttgat ccactttacc caggtggaag ctttgatcca ttgggcttag
1621 ctgatgatcc agaagcattc gcagaattga aggtgaagga actcaagaac ggtagattag
1681 ccatgttctc aatgtttgga ttcttcgttc aagctattgt aactggaaag ggtcctttgg
1741 agaaccttgc tgatcatctt gcagacccag tcaacaacaa tgcatggtca tatgccacca
1801 actttgttcc cggaaaataa acactcttat atttatatgt ttttgtgata gtaatcttct
1861 tcccaattca atgtgaatta ttatcattat cattatcatg tgggtatgca taggttcact
1921 aatacaagat gatggatgct ttttttttac caaattttaa attttatgtt tcatgctttc
1981 cattgctaga cat
//