GenBank-Updates@genbank.bio.net (05/01/91)
LOCUS YSCENO1 741 bp ds-DNA PLN 01-MAY-1991
DEFINITION Yeast enolase 1 [phosphopyruvate hydratase, EC 4.2.1.11] gene
(ENO1), 5' end, and flank.
ACCESSION D00410 M27568 M29128
KEYWORDS enolase 1; phosphopyruvate hydratase; promoter.
SOURCE Saccharomyces cerevisiae (strain AH22) chromosomal DNA, clone
pMC1587-ENO-C.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 741)
AUTHORS Uemura,H., Jigami,Y., Tanaka,H., Toshimitu,N., Paterson,M. and
Nakasato,S.
TITLE Nucleotide sequence of the 5' flanking region responsible for the
enhancement of the expression of yeast enolase 1 gene
JOURNAL J. Biochem. 98, 859-862 (1985)
STANDARD full staff_entry
REFERENCE 2 (sites)
AUTHORS Uemura,H., Shiba,T., Paterson,M., Jigami,Y. and Tanaka,H.
TITLE Identification of a sequence containing the positive regulatory
region of Saccharomyces cerevisiae gene EN01
JOURNAL Gene 45, 67-75 (1986)
STANDARD full staff_review
COMMENT This sequence of ENO1 5' flanking region is very important for
strong promoter function of ENO1.
FEATURES Location/Qualifiers
misc_feature 28..37
/note="palindromic structure"
CDS 36..188
/note="ORF"
/codon_start=36
misc_feature 77..84
/note="palindromic structure"
misc_feature 82..89
/note="palindromic structure"
repeat_region 158..191
/note="inverted repeat"
CDS 188..265
/note="ORF"
/codon_start=188
misc_feature 194..223
/note="AT rich stretch"
TATA_signal 593..599
/gene="EN01"
/citation=Gene 45, 67-75 (1986)
mRNA (698.701)..741
/product="enolase 1"
/gene="EN01"
/citation=Gene 45, 67-75 (1986)
/partial
misc_feature 701..704
/note="CAAG box"
/gene="EN01"
/citation=Gene 45, 67-75 (1986)
CDS 739..741
/product="enolase 1"
/gene="EN01"
/codon_start=739
/citation=Gene 45, 67-75 (1986)
/partial
BASE COUNT 220 a 188 c 133 g 200 t
ORIGIN 1 bp upstream of EcoRI site.
1 ctgcagcttt tgcccgggcg gtcattgatg catgcatgtg ccgtgaagcg ggacaaccag
61 aaaagtcgtc tataaatgcc ggcacgtgcg atcatcgtgg cggggtttta agagtgcata
121 tcacaaattg tcgcattacc gcggaaccgc cagatattca ttacttgacg caaaagcgtt
181 tgaaataatg acgaaaaaga aggaagaaaa aaaaagaaaa ataccgcttc taggcgggtt
241 atctactgat ccgagcttcc actaggatag cacccaaaca cctgcatatt tggacgacct
301 ttacttacac caccaaaaac cactttcgcc tctcccgccc ctgataacgt ccactaattg
361 agcgattacc tgagcggtcc tcttttgttt gcagcatgag acttgcatac tgcaaatcgt
421 aagtagcaac gtctcaaggt caaaactgta tggaaacctt gtcacctcac ttaattctag
481 ctagcctacc ctgcaagtca agaggtctcc gtgattccta gccacctcaa ggtatgcctc
541 tccccggaaa ctgtggcctt ttctggcaca catgatctcc acgatttcaa catataaata
601 gcttttgata atggcaatat taatcaaatt tattttactt ctttcttgta acatctctct
661 tgtaatccct tattccttct agctattttt cataaaaaac caagcaactg cttatcaaca
721 cacaaacact aaatcaaaat g
//