GenBank-Updates@genbank.bio.net (05/02/91)
LOCUS POL2LAN 7440 bp ss-RNA VRL 02-MAY-1991
DEFINITION Poliovirus type 2 (Lansing strain), complete genome.
ACCESSION M12197
KEYWORDS RNA polymerase; capsid protein; complete genome;
nonstructural protein; polymerase; protease.
SOURCE Poliovirus type 2 (Lansing strain), cDNA to viral RNA, clone
pVR204.
ORGANISM Poliovirus type 2
Viridae; ss-RNA nonenveloped viruses; Isometric ss-RNA viruses;
Picornaviridae; Enterovirus.
REFERENCE 1 (bases 1 to 7440)
AUTHORS La Monica,N., Meriam,C. and Racaniello,V.R.
TITLE Mapping of sequences required for mouse neurovirulence of
Poliovirus type 2 Lansing
JOURNAL J. Virol. 57, 515-525 (1986)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 7440)
AUTHORS Racaniello,V.R.
TITLE Revision
JOURNAL Unpublished (1991)
STANDARD full staff_review
COMMENT Computer readable copy of sequence kindly provided via
electronic mail by V.R. Racaniello, 07-MAY-1986.
FEATURES Location/Qualifiers
virion 1..7440
/note="pv RNA"
CDS 748..7371
/note="poliovirus polyprotein"
/codon_start=748
mat_peptide 748..954
/note="capsid protein VP4"
/codon_start=748
mat_peptide 955..1767
/note="capsid protein VP2"
/codon_start=955
mat_peptide 1768..2481
/note="capsid protein VP3"
/codon_start=1768
mat_peptide 2482..3384
/note="capsid protein VP1"
/codon_start=2482
mat_peptide 3385..3831
/note="protease 2A"
/codon_start=3385
mat_peptide 3832..4122
/note="nonstructural protein 2B"
/codon_start=3832
mat_peptide 4123..5109
/note="nonstructural protein 2C"
/codon_start=4123
mat_peptide 5110..5370
/note="nonstructural protein 3A"
/codon_start=5110
mat_peptide 5371..5436
/note="genome-linked protein VPg"
/codon_start=5371
mat_peptide 5437..5985
/note="protease 3C"
/codon_start=5437
mat_peptide 5986..7368
/note="RNA polymerase 3D"
/codon_start=5986
BASE COUNT 2163 a 1753 c 1722 g 1802 t
ORIGIN 631 bp upstream of BalI site.
1 ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcca gtacactggt
61 attgcggtac ctttgtacgc ctgttttata ctcccttccc ccgtaactta gaagcacaat
121 gtccaagttc aataggaggg ggtacaaacc agtaccacca cgaacaagca cttctgttcc
181 cccggtgagg ctgtataggc tgtttccacg gctaaaagcg gctgatccgt tatccgctca
241 tgtacttcga gaagcctagt atcaccttgg aatcttcgat gcgttgcgct caacactcaa
301 ccccagagtg tagcttaggt cgatgagtct ggacgttcct caccggcgac ggtggtccag
361 gctgcgttgg cggcctacct gtggcccaaa gccacaggac gctagttgtg aacaaggtgt
421 gaagagccta ttgagctacc tgagagtcct ccggcccctg aatgcggcta atcctaacca
481 cggagcaggc agtggcaatc cagcgaccag cctgtcgtaa cgcgcaagtt cgtggcggaa
541 ccgactactt tgggtgtccg tgtttccttt tatttttaca atggctgctt atggtgacaa
601 tcattgattg ttatcataaa gcaaattgga ttggccatcc ggtgagaatt tgattattaa
661 attactctct tgttgggatt gctcctttga aatcttgtgc actcacacct attggaatta
721 cctcattgtt aagatatcat caccactatg ggcgcccaag tctcatcaca gaaagttgga
781 gcccatgaga attcaaacag agcttatggc ggatccacca ttaattacac tactattaat
841 tattacaggg attctgcgag caatgccgct agtaagcagg actttgcaca agacccatcc
901 aagttcactg aacctattaa agatgttctc attaagaccg ctcccacact aaactctcct
961 aatatcgagg cgtgtgggta tagcgacaga gtgatgcaac taaccctagg caattccacc
1021 attaccacac aggaggcggc caattctgtc gttgcatacg gccggtggcc cgagtacatc
1081 aaggactcag aagcaaatcc tgtggaccag ccaactgaac cggacgttgc cgcgtgcagg
1141 ttttacacac tagacactgt tacttggcgc aaggagtcca gagggtggtg gtggaaactg
1201 cctgatgcac taaaggacat gggattattc ggccagaaca tgttctacca ctacctcggg
1261 agggctggct atactgtgca cgtacagtgt aatgcttcaa agtttcacca gggcgccctc
1321 ggggtattcg cagttccaga aatgtgcctg gcaggcgaca gcacaaccca catgtttaca
1381 aaatatgaga atgcaaatcc gggtgagaaa gggggtgaat tcaaagggag ttttactctg
1441 gatactaacg ctaccaaccc tgcacgcaac ttttgtcccg ttgattatct cttcgggagc
1501 ggagtactgg cgggaaatgc gtttgtttac ccacatcaga taattaatct gcgcaccaac
1561 aactgtgcca cgttggtgct gccatacgtt aattcacttt ccatagacag catgacaaaa
1621 cacaacaatt ggggaattgc tatccttccg ctggcaccac ttgactttgc caccgagtcc
1681 tccactgaga tacccattac tctaactatt gcccctatgt gttgtgaatt caatgggttg
1741 cgcaacatca ctgtacccag aactcaaggg ttgccagtct taaacactcc aggaagcaac
1801 cagtacttaa cagcagacaa ctatcaatcc ccatgtgcga tacccgagtt tgatgtaaca
1861 ccacccatag acatcccggg ggaagtgcgc aacatgatgg aattggcaga gatagacacc
1921 atgatacctc tcaatctgac gaaccagcgc aagaacacca tggatatgta cagagtcgaa
1981 ctgaatgatg cggctcactc tgacacacca atattgtgtc tctcactgtc tccagcatca
2041 gatcctaggc tagcacacac tatgctaggt gaaatactga actactacac acactgggca
2101 gggtcattga agttcacatt tctcttctgc ggctcaatga tggccactgg taaattgcta
2161 gtgtcctatg cacctcctgg tgcggaagcc cctaaaagcc gcaaagaagc gatgctcggc
2221 acccacgtga tctgggacat cggattacag tcatcatgca ctatggtggt accttggatt
2281 agcaacacca catacagaca aaccatcaac gatagcttca cagaaggagg gtacatcagt
2341 atgttttacc aaactagagt tgttgtgcca ttgtccaccc ctagaaagat ggacatattg
2401 ggctttgtgt cagcctgcaa tgacttcagt gtgcgcctgt tgcgtgacac gacgcacata
2461 agccaagagg ctatgccaca aggattgggt gatttaattg aaggggttgt tgagggagtc
2521 acgagaaatg ccttgacacc actgacacct gccaacaact tgcctgatac acaatctagc
2581 ggcccagccc actctaagga aacaccagcg ctaacagccg tagagacagg ggccaccaac
2641 ccattggtgc cttcagacac ggtacaaact cgtcacgtca tccaaaagcg gacgcggtcg
2701 gagtctacgg ttgagtcttt cttcgcaaga ggagcttgtg tggccattat tgaagtggat
2761 aatgatgctc caacaaagcg tgccagtaaa ttattttcag tctggaagat aacttacaaa
2821 gacaccgttc agttaagacg taagttggag ttctttacat attcaaggtt tgacatggag
2881 ttcacctttg tggttacatc caattatacc gatgcaaaca atgggcacgc actaaatcaa
2941 gtttaccaga taatgtacat accacctggg gcaccgatcc ctggcaagtg gaatgattac
3001 acatggcaaa cgtcatctaa cccatcagtg ttttacactt acggggcacc tccagctaga
3061 atatcagtgc cctacgtggg cattgccaat gcatattctc atttttacga tgggtttgcc
3121 aaagtaccac tagcaggcca agcctcaaca gagggtgact cgctgtatgg agcggcttca
3181 ttgaatgact tcggatcact ggctgttcga gtggtgaatg accacaaccc tacgaaactc
3241 acctcaaaaa tcagagtgta catgaaacca aagcacgtca gagtgtggtg tccgcgaccc
3301 cctcgagcag tcccatacta cggaccaggg gttgactaca aggatggact agccccactg
3361 ccagggaaag gcttgacaac ctatggtttt ggccaccaaa ataaggcagt gtacacggca
3421 ggttacaaaa tttgcaatta ccacctcgcc acccaggaag acttacaaaa tgcggtaaac
3481 attatgtgga ttagagacct tttagtagtg gaatccaaag cccaaggcat agactcaatt
3541 gctagatgta actgccacac tggagtgtac tactgtgaat ccaggaggaa gtactacccg
3601 gtctctttta ctggccccac ctttcagtac atggaagcaa atgagtacta tccagcccga
3661 taccaatccc acatgttaat tggccatggt tttgcatctc caggggactg tggtgggatt
3721 ctcaggtgcc aacatggagt aattggaatc attacagctg gaggagaagg cctagtcgct
3781 ttctcggaca tcagagatct gtacgcatac gaggaggagg ctatggagca gggagtctcc
3841 aactatattg agtcccttgg ggctgcattt gggagtggat tcacccagca aataggaaac
3901 aaaatttcag aactcactag catggtcacc agcactataa ctgagaaact actaaagaat
3961 ctcattaaaa taatttcatc ccttgttatc atcaccagaa actatgaaga cacgaccaca
4021 gtgctggcta cccttgctct cctcggttgt gatgcgtccc catggcaatg gctaaagaag
4081 aaagcctgtg acatcttgga aatcccctac atcatgcgac agggcgatag ctggttgaag
4141 aagtttacag aggcatgcaa tgcagctaag ggattggaat gggtgtctaa taaaatatcc
4201 aaatttattg actggctcaa agagaagatc attccacagg ctagagacaa gctagagttt
4261 gttaccaaac tgaagcaact agaaatgttg gagaaccaaa ttgcaaccat tcatcaatcg
4321 tgcccaagtc aggagcatca agaaatcctg ttcaataacg tgagatggtt atccatacag
4381 tcaaagagat ttgccccgct ctatgcggtt gaggctaaga gaatacaaaa gttagagcac
4441 acgattaaca actacgtaca gttcaagagc aaacaccgta ttgaaccagt atgtttgttg
4501 gtgcacggta gcccaggcac gggcaagtca gttgccacca atttaattgc cagagcaata
4561 gcagagaagg agaacacctc cacatactca ctaccaccag atccctccca tttcgatggg
4621 tacaagcaac aaggtgtggt gatcatggat gatttgaatc agaacccaga cggagcagac
4681 atgaagctgt tttgtcagat ggtctccact gtagaattca taccaccaat ggcttcgcta
4741 gaagaaaagg gtattttgtt cacatctaat tacgttttgg cctcaaccaa ttccagtcgc
4801 atcaccccac caactgttgc gcacagcgat gccctagcca ggcgctttgc atttgacatg
4861 gacatacaaa tcatgagcga gtattctaga gatggaaaat tgaacatggc gatggcaact
4921 gaaatgtgta agaactgtca tcacccagca aacttcaaga gatgttgccc attggtgtgt
4981 ggcaaagcca tccagctgat ggacaaatct tccagagtca gatatagtat agatcagatt
5041 actaccatga ttattaatga gaggaacaga agatcaagta tcggtaattg catggaggca
5101 cttttccaag gtcctcttca atacaaagac ctgaaaatag acattaagac cacacctcct
5161 cctgagtgca tcaatgattt gctccaagca gttgattctc aagaggtaag agactactgt
5221 gagaagaagg gttggatagt agacatcact agtcaggtgc aaaccgaaag aaacatcaat
5281 agagcaatga ctattcttca ggcggtcacc acatttgccg cagttgctgg agtggtgtat
5341 gtgatgtaca aactctttgc agggcatcaa ggagcgtata cagggcttcc caataagaga
5401 cccaatgtcc ccaccatcag gactgccaag gttcagggcc caggatttga ctacgcagtg
5461 gcaatggcca aaagaaacat tcttacggca actaccatta agggagagtt cacaatgctc
5521 ggagtgcatg ataatgtggc cattctacca acccacgcat caccgggtga aacaatagtc
5581 attgatggca aggaagtaga ggtactggat gctaaagccc tggaggacca ggccgggacc
5641 aacctggaaa tcaccattgt cactcttaag agaaatgaga agttcaggga catcagacca
5701 cacatcccca ctcaaatcac tgagacaaat gatggagttt taattgtgaa cactagtaag
5761 taccccaaca tgtatgttcc tgtcggtgct gtgactgaac aggggtatct caatctcagt
5821 ggacgccaaa ctgctcgtac tttaatgtac aactttccaa cgagagcagg tcaatgtggt
5881 ggagttatca cctgcactgg caaggtcatc gggatgcatg ttggtgggaa cggttcacat
5941 gggttcgcag cagccctgaa gcgatcctat ttcactcaga gtcaaggtga aatccagtgg
6001 atgagaccat caaaagaagt gggctacccc gttattaatg ctccatctaa aactaaactg
6061 gaacccagtg cattccatta tgtgtttgaa ggtgtcaagg aaccagctgt gctcaccaaa
6121 agtgacccca gattgaagac agattttgaa gaggctatct tttccaagta tgtgggaaat
6181 aagattactg aagtggatga gtacatgaaa gaagctgtcg atcattacgc aggccagctc
6241 atgtcactag acatcaacac agaacaaatg tgccttgagg atgcaatgta tggcactgac
6301 ggtctcgaag ctctagacct cagtaccagt gctgggtatc cctatgtggc aatggggaaa
6361 aagaaaagag acattttgaa taagcaaacc agagacacaa aggaaatgca aaggcttctg
6421 gacacctatg gtattaattt acctttagtc acctatgtga aagatgagct tagatccaag
6481 accaaagtgg aacagggcaa gtccaggcta attgaggcct caagtctcaa tgactctgtc
6541 gccatgagga tggcttttgg caacttgtac gcagcattcc acaagaaccc aggtgtagtg
6601 acaggatcgg ctgttggctg tgacccagat ttgttttgga gtaaaatacc agtcctcatg
6661 gaggaaaaac tctttgcatt tgattacacg ggttatgatg cttcactaag ccccgcctgg
6721 tttgaggctc tcaagatggt tctagagaaa attgggtttg gtgacagagt ggattacatt
6781 gattatctga atcactcgca ccatctatat aaaaataaga catattgtgt taagggcggc
6841 atgccatctg gctgctctgg cacctcaatt tttaattcaa tgattaataa tctaataatc
6901 aggactctct tactgaaaac ctacaagggc atagatttag accacctgaa gatgatagcc
6961 tatggtgatg atgtaattgc ttcctacccc catgaggttg atgctagtct cctagcccaa
7021 tcaggaaaag actatggact aaccatgaca ccagctgaca aatcagccac ctttgaaaca
7081 gtcacatggg agaatgtaac attcttgaaa agattcttta gagcagatga aaagtatccc
7141 tttctggtac atccagtgat gccaatgaaa gaaattcacg aatcaattag atggactaaa
7201 gatcccagaa acactcagga tcatgttcgc tcactgtgct tattggcttg gcacaatggc
7261 gaggaagagt acaataaatt tttagctaag attagaagtg tgccaatcgg aagagcatta
7321 ctgctccctg agtactccac attgtaccgc cgttggctcg actcatttta gtaaccctac
7381 ctcagtcgaa ttggattggg tcatactgtt gtaggggtaa atttttcttt aattcggagg
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