GenBank-Updates@genbank.bio.net (05/02/91)
LOCUS MBOPILINB 3051 bp ds-DNA BCT 02-MAY-1991
DEFINITION M.bovis pilin subunit protein (tfpI) pseudogene, tfpB gene, and
pilin subunit gene (tfpQ), complete cds.
ACCESSION M59712
KEYWORDS pilin; pilin gene inverting protein; pseudogene; type 4 pilin.
SOURCE M.bovis (strain Epp63) DNA.
ORGANISM Moraxella bovis
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE 1 (bases 1 to 3051)
AUTHORS Marrs,C.F. and Rozsa,F.W.
TITLE Interesting sequence differences between the pilin gene inversion
regions of Moraxella lacunata ATCC 17956 and Moraxella bovis Epp63
JOURNAL Unpublished (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
misc_feature 31..58
/note="This is the 5' inversion junction site flanking a
2.1 kb invertible region. It has homology to the Hin
family of junction sites, particularily Cin."
/evidence=EXPERIMENTAL
CDS 34..459
/pseudo
/note="This is a partial pilin gene lacking the amino
terminus. Inversion of a 2.1 kb region occurs such that
this gene is joined to the conserved amino terminus. The
functional gene produces an alternative pilin subunit
protein for pili assembly."
/gene="tfpI"
/evidence=EXPERIMENTAL
/codon_start=34
/partial
CDS complement(771..1556)
/note="This gene has a potential transcription terminator
(1687-1714) but no known promoter, it may be transcribed
off the pilin subunit gene promoter."
/product="TfpB protein"
/gene="tfpB"
/codon_start=1556
terminator complement(1687..1714)
/function="transcription terminator for tfpQ"
/evidence=EXPERIMENTAL
CDS complement(1737..2210)
/note="This gene produces a functional type pilin gene
subunit."
/product="pilin subunit"
/gene="tfpQ"
/codon_start=2210
misc_feature 2133..2158
/note="This is the 3' inversion junction site. It has
homology to the Cin junction site. The amino portion of
the expressed pilin subunit gene goes through this
region."
/evidence=EXPERIMENTAL
BASE COUNT 966 a 595 c 597 g 893 t
ORIGIN
1 cccttttgac aaacgcattc atgaataaca tttattggta tcctagctgc aatcgcttta
61 cctgcttacc aagactatat ctctaagtct caaactactc gtgtttctgg tgagctagct
121 gctggtaaaa ctgctgtaga cgctgctcta tttgaaggta aaactcctgt gctaagtgaa
181 gaatctagca cttcaaaaga gaatattggt ctgactagct cagaaactag cacaaaacca
241 cgttccaatc tgatggcttc tgttgaacta actggttttg ctgacaatgg tgcaggtact
301 atttctgcta ctttgggtaa caaagctaat aaagacatcg ccaaaactgt tattacacaa
361 gaacgtacta ctgacggtgt atggacttgc aaaatcgatg gttctcaagc agctaaatac
421 aaagagaagt tcaacccaac tggttgtgtc aagaaataat ctcatagtag attgcaaaaa
481 ccactcctta tggggtggtt ttttacgtaa atacatcatc agcaaccact attaacactc
541 cttaaaagag cgattaacat caaatataag aaaaaccgcc actcaaagaa ccgttggcgt
601 accagctagg taaccacact caattcatat caaaatcata caaaaccgtt cgctctgagc
661 ctgcctgcgg gtgaagcggt tctccgttat gggcgggcat agcttaccat aaacggaaaa
721 cctttgttgt gctttgaata gaatttacca tacaaattgt caaatattgc ttattcttgg
781 tggtatattt gtgctgatag ttggttttta tagggtaggt aaggagatgg tgtgatgtgt
841 atggtgtaat gatttaaggt ttttcgatta aaaaagataa aatgactgtt atgcttgatg
901 ttctcaccca agataaactc ttcatcaccg tttgccacca agatataata agggcgattc
961 tctacttcaa taatatcata acctgtaaaa aaggctgaac gcaaataccc tatcgaaaac
1021 gcagaaacga ccacatacac aaaaataaaa gccataaaaa tctgataata tttttcgtta
1081 ctcatcaact ccctaagacg aatagaaaaa gagattgccc gtccgaactt atggcaaact
1141 aaagaaatgg ctaaaataga aagcacacat cccatcagac ggtatgacga caaaatacca
1201 acataaaaat acagcagcaa catgatgggt gttgatacaa tcaccaataa aataaaaatt
1261 ttgacaaacc ccaaatgaga tgcaccccaa gagacatgta gtagcccaaa aaatctctta
1321 ataaacttaa acgcctgata tccgatcaaa tagccaataa caaaaaccat ggataccaca
1381 cagacataag ccaaacttct ggcaatcatg ctaccacctt gcaccacatg ccaccatggg
1441 tagccatgat atgctgcttg accccaacca taagcgtaag ccgtcgccca acttaataca
1501 gccaaataca ccattgatac cgacgaggcg acaaatttat taaaattatt tagcataact
1561 taagtatttt ttaaaacaag aataaaaaga aatggatgat aattgtgatt gattatttta
1621 gataaaaaat agacaagtgt tttcttattt ttgtatattt attgtaaata ttatgtcttt
1681 atgttaaaaa acaccactcc aagggagtgg tgttgttgtg atttgcaatc agatgattac
1741 tctttacaac cagtaggtac gaacttagat ttccaacctg gagcggcaga accgttaatg
1801 gtgcaggtcc atacaccttc cgcatcacga gattgagtaa tgacagcacc agcgatgtct
1861 ttattggcac ggttacccaa agtagcttct agagtgccag caccagttgc aaatgcacca
1921 ccaccgatat taacactaga catcaagtta gaacgagcgg taccgccagt agtggtaagg
1981 ccgatgtctt cttcagtgtc gttagcagct ttacctagtt tgggagtttt accctcaaac
2041 aaagcagcat ccacagcagt tttaccagca gctagttcgc caactacacg agtagtttga
2101 gacttagaga tatagtcttg gtaagcaggt agagcgattg cagctaggat accaataatg
2161 gcgataacaa tcatcaattc gataagggtg aaaccttttt gagcgttcat aatgaactcc
2221 ttagttcata agggcgatat tgcccagttt gtttaagtta actgattagc aattttaaac
2281 atatttgcta accttacact catactgttg cattgtctgt gccaactttt tagcggattt
2341 gggctgtttt tggaaaaaat acgcaaaatt gagaaaattt taaagatttt gtctaaaaaa
2401 taggcatatt ttttgctatt ttttaaaatt gattaaaata tgtacaattt ttttcttttt
2461 taccaaaaaa tttttaaaat ttgatgtctg gcgggtaatt ttgggtgaca atttttgtca
2521 gttttggatt tttcgacatt ttttggcagg ttggactgac attttagcag agatgtcaat
2581 ttttgacatg gacaaaattt ttacctgtca aaaattgttg ccaaagtttg tcatcttggc
2641 tgattttgga cacaaatgaa cactgacatg gtattaatgt gttgtatgtg acgatttttg
2701 tcaatgttga taaactggca taagatgatg tgaaacgggt gatcatcaca gatccatggc
2761 gaacatcaga ttcgctgggt gtaaatttat ttttatcaaa aaagccccga acatgttcgg
2821 ggctttggtg atggtggatt agtacttttg gtcttcgggg gcttgctgtt cggcggtggc
2881 ggtgttgcct gttacggtat cagtggcact gctggcagcc cctgtcacgg catcggcggt
2941 ggcggtagcg gcatcagaga ccacgcttgc ggcagcttcg gcggtatttt tggcattttc
3001 tacgccatta tttgccacgt catgagctac attagagaca ccttcagcgg t
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