GenBank-Updates@genbank.bio.net (05/02/91)
LOCUS STMKAMB 850 bp ds-DNA BCT 02-MAY-1991
DEFINITION Streptomyces tenebrarius kanamycin-apramycin resistance methylase
(KAMB) gene, complete cds.
ACCESSION M64625
KEYWORDS S-adenosyl methionine; kanamycin-apramycin resistance methylase.
SOURCE Streptomyces tenebrarius (strain NCIB 11028) DNA.
ORGANISM Streptomyces tenebrarius
Prokaryota; Bacteria; Firmicutes; Streptomycetaceae.
REFERENCE 1 (bases 1 to 850)
AUTHORS Holmes,D.J., Drocourt,D., Tiraby,G. and Cundliffe,E.
TITLE Cloning of an aminoglycoside-resistance-encoding gene, KAMC, from
Saccharopolyspora hirsuta: Comparison with KAMB from Streptomyces
tenebrarius
JOURNAL Gene (1991) In press
STANDARD full staff_entry
FEATURES Location/Qualifiers
mRNA 3..850
/note="transcript start point 1"
/gene="KAMB"
mRNA 124..850
/note="transcript start point 2"
/gene="KAMB"
RBS 171..178
/gene="KAMB"
CDS 183..647
/product="kanamycin-apramycin resistance methylase"
/gene="KAMB"
/codon_start=183
BASE COUNT 114 a 315 c 314 g 107 t
ORIGIN
1 gcatgcgccg cgtggtgggc aagcgggtcc aggagttctc cgacgccgag ttcgagcagc
61 tacggagtca gtacgacgac gtggtgctcg acgtcggcac cggcgacggg aagcatccgt
121 acaaggtcgc ccgccagaac ccctcccggc tggtggtggc gctcgacgcc gacaagagcc
181 ggatggagaa gatctcggcg aaggcggcgg ccaagcccgc gaaggccctg cccaacctgc
241 tgtacctgtg ggccaccgcc gagcggctcc ccccgttgtc gggggtgggc gagctgcacg
301 tcctcatgcc gtggggcagc ctgctgcgcg gggtcctcgg ctcctcgccg gagatgctgc
361 gcgggatggc ggcggtgtgc cggccgggcg cgtccttcct ggtcgagtgc aaccgcagct
421 gccgtgggcc cccggtgccg gaggtgggcg agcaccccga gcccaccccg gactccgccg
481 acgagtggct ggcgccccgc tacgccgagg ccgggtggaa gctcgccgac tgccgctacc
541 tggagccgga ggaggtggcg ggtctggaga cctcctggac ccgccgtctg cactcctccc
601 gcgaccggtt cgacgtgctc gcgctcaccg gcacgatcag tccgtgacgc cggaccccgg
661 gcgtcgggcg ggcgtgaacg tccgccgcgc gccgggggcc agctcgacgc gcgtgtcgtc
721 gacccggacc ggcagcggca cgcgttgtcc gcgtcggcca gcaactccac ggtccccggg
781 gtgagcacca cggtcaaccc ccggccacgc caaccggcag cggaacccgc aagccgggtc
841 acgccctccg
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