GenBank-Updates@genbank.bio.net (05/02/91)
LOCUS SCSKAMC 850 bp ds-DNA BCT 02-MAY-1991
DEFINITION Saccharopolyspora hirsuta kanamycin-apramycin resistance methylase
(KAMC) gene, complete cds.
ACCESSION M64626
KEYWORDS S-adenosyl methionine; aminoglycosides resistance;
kanamycin-apramycin resistance methylase; oncogene;
transmembrane protein; tyrosine kinase.
SOURCE Saccharopolyspora hirsuta (strain CL 102) DNA.
ORGANISM Saccharopolyspora hirsuta
Prokaryota; Bacteria; Firmicutes; Nocardioform actinomycetes.
REFERENCE 1 (bases 1 to 850)
AUTHORS Holmes,D.J., Drocourt,D., Tiraby,G. and Cundliffe,E.
TITLE Cloning of an aminoglycoside-resistance-encoding gene, KAMC, from
Saccharopolyspora hirsuta: Comparison with KAMB from Streptomyces
tenebrarius
JOURNAL Gene (1991) In press
STANDARD full staff_entry
FEATURES Location/Qualifiers
RBS 203..207
/gene="KAMC"
CDS 212..682
/product="kanamycin-apramycin resistance methylase"
/gene="KAMC"
/codon_start=212
BASE COUNT 125 a 278 c 334 g 113 t
ORIGIN
1 gatcggggaa cggcggtctc tagactgggc gcatgcgcag ggtggtgggc aagaacgtcg
61 tggagttctc cgcggagagt tcgacgagat gttggaccgg tactccggcg gggtcgtgct
121 cgacgtcggc gcgggcaacg caagcacgcc taccagctcg cccgcggggc aggacacgtt
181 ggtgatgcgc gctcgacggg ccaaggacaa catgcagaag atcgccggga aggccgctgc
241 ttcgccgaag aagggcggtg cgccgaacct gctctgcgtg tgggcgtccg cggagaagcc
301 gccgccggag ctcgcgcgcg tcacggagct gcactcgctg atgccgtggg ggagcctgct
361 gcgcgggatg ctcgggtcgg acaccgagat gcggccgggg ctcgcggagc tctgcgtgcc
421 cggcgcggag ttcctgatca cgctcaacct gcacgcctgg cggccgccgg tccccgaggt
481 cggcgaccac ccggagccca ccccggagtc cgcgatggag ctgctcgccc cgtactacgc
541 ggcggccggg tggaccctgg aggaggcgcg gtacttcggc gccgaggagc tggacgcgct
601 ggccacctcg tggacccggc ggctggggtc gacccgcgac gagctcgacg tcctcgggat
661 cacgggcgtc atcgggaagt gacggcgatg ctggacgatc ccggaggcgt gctgctgcgc
721 atgaagtgcc gcgggcacgc cgacatccgc gccacgcacg ggaaaaccct ggagttcgcc
781 gccgatgcgg agatcaccca gcgggcgacg tgcgtcgtcg gggtggccgc cgaggtccgc
841 gagctcgccg
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