GenBank-Updates@genbank.bio.net (05/03/91)
LOCUS SYNECOPGEX 4932 bp ds-DNA SYN 03-MAY-1991
DEFINITION Plasmid pGEX-3 expression vector encoding a transformed glutathione
S-transferase (Sj26) gene, 5' end.
ACCESSION M21676
KEYWORDS fusion protein; glutathione S-transferase.
SOURCE E.coli DNA and S.japonicum DNA, clone pGEX-1.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 4932)
AUTHORS Smith,D.B. and Johnson,K.S.
TITLE Single-step purification of polypeptides expressed in Escherichia
coli as fusions with glutathione S-transferase
JOURNAL Gene 67, 31-40 (1988)
STANDARD full staff_entry
REFERENCE 2 (bases 1 to 4932)
AUTHORS Davern,K.M.
JOURNAL Unpublished (1991)
STANDARD full staff_review
COMMENT The protease cleavage site described by pGEX-3 is recognized by
factor X.
FEATURES Location/Qualifiers
CDS 258..944
/note="transformed"
/product="glutathione S-transferase"
/codon_start=258
/citation=[Publication] ??, ??-?? (1991)
misc_feature 914..919
/note="BamHI clonong site"
/citation=[Publication] ??, ??-?? (1991)
misc_feature 919..924
/note="SmaI clonong site"
/citation=[Publication] ??, ??-?? (1991)
misc_feature 924..929
/note="EcoRI clonong site"
/citation=[Publication] ??, ??-?? (1991)
BASE COUNT 1222 a 1190 c 1278 g 1242 t
ORIGIN 29 bp upstream of BamHI site.
1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
901 atcctccaaa atcggatccc cgggaattca tcgtgactga ctgacgatct gcctcgcgcg
961 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
1021 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
1081 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata attcttgaag
1141 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
1201 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
1261 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
1321 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
1381 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
1441 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
1501 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
1561 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
1621 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
1681 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
1741 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
1801 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
1861 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
1921 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
1981 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
2041 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
2101 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
2161 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
2221 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
2281 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
2341 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
2401 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
2461 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
2521 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
2581 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
2641 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
2701 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
2761 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
2821 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
2881 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
2941 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
3001 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
3061 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
3121 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
3181 tatttcacac cgcataaatt ccgacaccat cgaatggtgc aaaacctttc gcggtatggc
3241 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata
3301 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
3361 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
3421 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
3481 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
3541 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
3601 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
3661 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
3721 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
3781 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
3841 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
3901 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
3961 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
4021 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
4081 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgtcaac
4141 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
4201 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
4261 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
4321 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
4381 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
4441 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
4501 gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
4561 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
4621 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
4681 gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc
4741 gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc
4801 tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg
4861 ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
4921 ggcgttggaa tt
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