GenBank-Updates@genbank.bio.net (04/03/91)
LOCUS ECOTGT 1823 bp ds-DNA BCT 03-APR-1991
DEFINITION E.coli tRNA-guanine-transglycosylase (tgt) gene, complete cds.
ACCESSION M63939 M35286
KEYWORDS tRNA-binding cytoplasmic protein; tRNA-guanine-transglycosylase;
transglycosylase.
SOURCE E.coli K-12 DNA.
ORGANISM Escherichia K-12
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1823)
AUTHORS Reuter,K., Frey,B. and Kersten,H.
TITLE Structure, organization, and function of the tgt locus in
Escherichia coli K-12
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
-10_signal 122..127
/gene="tgt"
/evidence=EXPERIMENTAL
CDS 216..1343
/EC_number="2.4.2.29"
/product="tRNA-guanine-transglycosylase"
/gene="tgt"
/evidence=EXPERIMENTAL
/codon_start=216
-35_signal 1247..1252
/gene="tgt"
-10_signal 1271..1276
/gene="tgt"
/evidence=EXPERIMENTAL
CDS 1366..1698
/note="ORF"
/evidence=EXPERIMENTAL
/codon_start=1366
BASE COUNT 441 a 415 c 490 g 477 t
ORIGIN 9 min. on K-12 map.
1 gtcgacgctg attatgctgg tttcggcctt tgccggttat caacacacca tgaacgccta
61 taaagcagcg gtagaagaga aatatcgctt ttttagttac ggtgatgcga tgtttatcac
121 gtacaatccg caggcaatta atgagcgcgt cggggagtaa ttccgcggcg ctggtttaaa
181 acgttggact gtttttctga cgtagtggag aaaaaatgaa atttgaactg gacaccaccg
241 acggtcgcgc acgccgtggc cgcctggtct ttgatcgtgg cgtagtggaa acgccttgtt
301 ttatgcctgt tggcacctac ggcaccgtaa aagggatgac gccggaagaa gttgaagcca
361 ctggcgcgca aattatggtc ggcaacacct tccacctgtg gctgcgcccg ggccaggaaa
421 tcatgaaact gcacggcgat ctgcacgatt ttatgcagtg gaaggggccg atcctcaccg
481 actccggcgg cttccaggtc ttcagccttg gcgatattcg taaaatcacc gaacagggcg
541 tgcacttccg taacccgatc aacggcgatc cgattttcct cgatcctgaa aaatcaatgg
601 agattcagta cgatcttggt tcggatatcg tcatgatctt tgatgagtgt acgccgtatc
661 ctgctgactg ggattacgca aaacgctcca tggagatgtc tctgcgttgg gcgaagcgta
721 gccgtgagcg ttttgacagt ctcggaaaca aaaatgcgct gtttggtatc atccagggca
781 gcgtttacga agatttacgt gatatttctg ttaaaggtct ggtagatatc ggttttgatg
841 gctacgctgt cggcggtctg gctgtgggtg agccgaaagc agatatgcac cgcattctgg
901 agcatgtatg cccgcaaatt ccggcagaca aaccgcgtta cctgatgggc gttggtaaac
961 cagaagacct ggttgaaggc gtacgtcgtg gtatcgatat gtttgactgc gtaatgccaa
1021 cccgcaacgc ccgaaatggt catttgttcg tgaccgatgg cgtggtgaaa atccgcaatg
1081 cgaagtataa gagcgatact ggcccactcg atcctgagtg tgattgctac acctgtcgca
1141 attattcacg cgcttacttg catcatcttg accgttgcaa cgaaatatta ggcgcgcgac
1201 tcaacaccat tcataacctt cgttactacc agcgtttgat ggcgggttta cgcaaggcta
1261 ttgaagaggg taaattagag agcttcgtaa ctgattttta ccagcgtcag gggcgagaag
1321 taccaccttt gaacgttgat taatattaat aatgagggaa atttaatgag cttttttatt
1381 tctgatgcgg tagcggcaac gggtgcaccg gcgcaaggta gcccgatgtc tttgattttg
1441 atgctggtgg tattcggtct gattttctat ttcatgatcc tgcgtccaca gcagaagcgc
1501 accaaagaac acaaaaagct gatggactcc attgccaaag gtgatgaagt tctgacgaac
1561 ggtggcctgg ttggtcgcgt aaccaaagta gcggaaaacg gctacattgc tatcgcgctg
1621 aatgacacca ctgaagtagt tattaaacgt gacttcgtag ctgccgtcct gccgaaaggc
1681 accatgaagg cgctgtaatt aaaatttttc cctaagggaa ttgccgtgtt aaaccgttat
1741 cctttgtgga agtacgtcat gctgatcgtg gtgattgtca tcggtctgct gtatgcgctt
1801 cccaacctgt ttggtgagga tcc
//GenBank-Updates@genbank.bio.net (05/03/91)
LOCUS ECOTGT 1823 bp ds-DNA BCT 03-MAY-1991
DEFINITION E.coli tRNA-guanine-transglycosylase (tgt) gene, complete cds.
ACCESSION M63939 M35286
KEYWORDS tRNA-binding cytoplasmic protein; tRNA-guanine-transglycosylase;
transglycosylase.
SOURCE E.coli K-12 DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1823)
AUTHORS Reuter,K., Frey,B. and Kersten,H.
TITLE Structure, organization, and function of the tgt locus in
Escherichia coli K-12
JOURNAL J. Bacteriol. 173, 2256-2264 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 216..1343
/EC_number="2.4.2.29"
/product="tRNA-guanine-transglycosylase"
/gene="tgt"
/evidence=EXPERIMENTAL
/codon_start=216
CDS 1366..1698
/note="ORF"
/evidence=EXPERIMENTAL
/codon_start=1366
BASE COUNT 441 a 415 c 490 g 477 t
ORIGIN 9 min. on K-12 map.
1 gtcgacgctg attatgctgg tttcggcctt tgccggttat caacacacca tgaacgccta
61 taaagcagcg gtagaagaga aatatcgctt ttttagttac ggtgatgcga tgtttatcac
121 gtacaatccg caggcaatta atgagcgcgt cggggagtaa ttccgcggcg ctggtttaaa
181 acgttggact gtttttctga cgtagtggag aaaaaatgaa atttgaactg gacaccaccg
241 acggtcgcgc acgccgtggc cgcctggtct ttgatcgtgg cgtagtggaa acgccttgtt
301 ttatgcctgt tggcacctac ggcaccgtaa aagggatgac gccggaagaa gttgaagcca
361 ctggcgcgca aattatggtc ggcaacacct tccacctgtg gctgcgcccg ggccaggaaa
421 tcatgaaact gcacggcgat ctgcacgatt ttatgcagtg gaaggggccg atcctcaccg
481 actccggcgg cttccaggtc ttcagccttg gcgatattcg taaaatcacc gaacagggcg
541 tgcacttccg taacccgatc aacggcgatc cgattttcct cgatcctgaa aaatcaatgg
601 agattcagta cgatcttggt tcggatatcg tcatgatctt tgatgagtgt acgccgtatc
661 ctgctgactg ggattacgca aaacgctcca tggagatgtc tctgcgttgg gcgaagcgta
721 gccgtgagcg ttttgacagt ctcggaaaca aaaatgcgct gtttggtatc atccagggca
781 gcgtttacga agatttacgt gatatttctg ttaaaggtct ggtagatatc ggttttgatg
841 gctacgctgt cggcggtctg gctgtgggtg agccgaaagc agatatgcac cgcattctgg
901 agcatgtatg cccgcaaatt ccggcagaca aaccgcgtta cctgatgggc gttggtaaac
961 cagaagacct ggttgaaggc gtacgtcgtg gtatcgatat gtttgactgc gtaatgccaa
1021 cccgcaacgc ccgaaatggt catttgttcg tgaccgatgg cgtggtgaaa atccgcaatg
1081 cgaagtataa gagcgatact ggcccactcg atcctgagtg tgattgctac acctgtcgca
1141 attattcacg cgcttacttg catcatcttg accgttgcaa cgaaatatta ggcgcgcgac
1201 tcaacaccat tcataacctt cgttactacc agcgtttgat ggcgggttta cgcaaggcta
1261 ttgaagaggg taaattagag agcttcgtaa ctgattttta ccagcgtcag gggcgagaag
1321 taccaccttt gaacgttgat taatattaat aatgagggaa atttaatgag cttttttatt
1381 tctgatgcgg tagcggcaac gggtgcaccg gcgcaaggta gcccgatgtc tttgattttg
1441 atgctggtgg tattcggtct gattttctat ttcatgatcc tgcgtccaca gcagaagcgc
1501 accaaagaac acaaaaagct gatggactcc attgccaaag gtgatgaagt tctgacgaac
1561 ggtggcctgg ttggtcgcgt aaccaaagta gcggaaaacg gctacattgc tatcgcgctg
1621 aatgacacca ctgaagtagt tattaaacgt gacttcgtag ctgccgtcct gccgaaaggc
1681 accatgaagg cgctgtaatt aaaatttttc cctaagggaa ttgccgtgtt aaaccgttat
1741 cctttgtgga agtacgtcat gctgatcgtg gtgattgtca tcggtctgct gtatgcgctt
1801 cccaacctgt ttggtgagga tcc
//