GenBank-Updates@genbank.bio.net (04/25/91)
LOCUS ECOEBGRA 4983 bp ds-DNA BCT 25-APR-1991
DEFINITION E.coli ebgR, ebgA, ebgC genes complete cds.
ACCESSION M64441
KEYWORDS EBG enzyme alpha subunit; EBG enzyme beta subunit; EGB repressor;
ebgA gene; ebgC gene; ebgR gene.
SOURCE E.coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Stokes,H.W., Betts,P.W. and Hall,B.G.
TITLE Sequence of the ebgA gene of Escherichia coli: Comparison with the
lacZ gene
JOURNAL Mol. Biol. Evol. 2, 469-477 (1985)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Stokes,H.W. and Hall,B.G.
TITLE Sequence of the ebgR gene of Escherichia coli: Evidence that the
EBG and LAC operons are descended from a common ancestor
JOURNAL Mol. Biol. Evol. 2, 478-483 (1985)
STANDARD full staff_review
REFERENCE 3 (bases 1 to 4983)
AUTHORS Hall,B.G., Betts,P.W. and Wootton,J.C.
TITLE DNA sequence analysis of artificially evolved ebg enzyme and ebg
repressor genes
JOURNAL Genetics 123, 635-648 (1989)
STANDARD full automatic
FEATURES Location/Qualifiers
CDS 126..1109
/product="EBG repressor"
/gene="ebgR"
/codon_start=126
mutation 695..695
/note="'ebgR, results in increased sensitivity to
inducers'"
/note="'G' in mutation"
mutation 708..708
/note="'ebgR, results in increased sensitivity to
inducers'"
/note="'A' in mutation"
mutation 712..712
/note="'ebgR, results in increased sensitivity to
inducers'"
/note="'G' in mutation"
mutation 760..761
/note="'ebgR-, ochre, constitutive synthesis of EBG
enzyme'"
/note="'AA' in mutation"
mutation 1021..1021
/note="'ebgR-, singe base insertion causes frameshift,
constitutive'"
/note="'TT' in mutation"
CDS 1293..4388
/product="EBG enzyme alpha subunit"
/gene="ebgA"
/codon_start=1293
mutation 1566..1566
/note="'ClassI mutation site in ebgA'"
/note="'A' in mutation"
mutation 4223..4223
/note="'Class II site in ebgA'"
/note="'T' in mutation"
mutation 4227..4227
/note="'Class V site in ebgA'"
/note="'G' in mutation"
CDS 4385..4903
/product="EGB enzyme beta subunit"
/gene="ebgC"
/codon_start=4385
mutation 4749..4749
/note="'ebgC mutation present in some Class IV alleles'"
/note="'G' in mutation"
BASE COUNT 1245 a 1338 c 1269 g 1131 t
ORIGIN
1 ttcgtgagcc gctgtgttac cgttacagcg tcaaagaaac gcgctttatt tactgaaaac
61 aggtgacccg ataagcactt cctctacaat gggggcgcac atcagggaaa gtaaaaaagg
121 taaacatggc aacactaaaa gacatcgcaa tcgaagctgg cgtatccctg gcgacagtat
181 ccagggtctt aaatgacgat ccgacattga atgtgaaaga agagacgaaa catcgcattc
241 tcgagatcgc cgaaaagctg gagtacaaga ccagtagtgc ccgtaaactc cagacaggtg
301 cagtcaacca acaccatatt ctggctatct acagctacca gcaggagctg gagatcaacg
361 atccttacta tctggcgatc cgccacggca ttgaaaccca gtgcgaaaag ctgggcatcg
421 agctcaccaa ctgttatgaa cacagcggct taccagacat taaaaacgtc accggtattt
481 taattgtcgg caaacccacg cccgccctgc gcgccgctgc cagcgcgttg accgacaata
541 tctgttttat cgactttcac gaacccggca gcggttacga tgcggtggat atcgatctgg
601 cacgcatcag taaagaaatc atcgacttct atatcaacca gggcgttaat cgtattggtt
661 ttattggcgg tgaagatgag cctggcaagg cggatattcg tgaggtcgcc tttgcggaat
721 atggccgact gaaacaagtg gtacgcgaag aggatatctg gcgcggcggt ttttccagtt
781 cgtcgggtta tgaactggca aaacaaatgc tggcgcggga agactatccg aaggcactgt
841 ttgttgcttc cgattccatt gctatcggcg tactgcgggc aattcatgaa cgaggcctga
901 acatcccaca ggatatttcg cttatcagcg ttaacgatat ccccaccgcg cgatttacct
961 ttccgccgct ctccaccgtg cgcatccatt ccgagatgat gggaagtcag ggcgttaacc
1021 tggtgtatga aaaagcccgc gatggtcgcg cgctgccgct gttagtcttc gttcccagca
1081 aattaaaact gcgcggcacg acccgttaaa tccccttaca cactgtccgg caatcgtttt
1141 tgccggacag tgctgccgtt tattttcgtg atccagttaa agtaaatgca tttacctgct
1201 actttttagt aaaaatttta ctaaactccc cagcaattac acaaactacc atcaccatga
1261 atggttccga tttctctcta ccgggaggcc ctatgaatcg ctgggaaaac attcagctca
1321 cccacgaaaa ccgacttgcg ccgcgtgcgt actttttttc atatgattct gttgcgcaag
1381 cgcgtacctt tgcccgcgaa accagcagcc tgtttctgcc cttaagcggt cagtggaatt
1441 tccacttttt tgaccatccg ctgcaagtac cagaagcctt cacctctgag ttaatggctg
1501 actgggggca tattaccgtc cccgccatgt ggcaaatgga aggtcacggc aaactgcaat
1561 ataccgacga aggttttccg ttccccatcg atgtgccgtt tgtccccagc gataacccaa
1621 ccggtgccta tcaacgtatt ttcaccctca gcgacggctg gcagggtaaa cagacgctga
1681 ttaaatttga cggcgtcgaa acctattttg aagtctatgt taacggtcag tatgtgggtt
1741 tcagcaaggg cagtcgcctg accgcagagt ttgacatcag cgcgatggtt aaaaccggcg
1801 acaacctgtt gtgtgtgcgc gtgatgcagt gggcggactc tacctacgtg gaagaccagg
1861 atatgtggtg gtcagcgggg atcttccgcg atgtttatct ggtcggaaaa cacctaacgc
1921 atattaacga tttcactgtg cgtaccgact ttgacgaagc ctattgcgat gccacgcttt
1981 cctgcgaagt ggtgctggaa aatctcgccg cctcccctgt cgtcacgacg ctggaatata
2041 ccctgtttga tggcgaacgc gtggtgcaca gcagcgccat tgatcatttg gcaattgaaa
2101 aactgaccag cgccacgttt gcttttactg tcgaacagcc gcagcaatgg tcagcagaat
2161 ccccttatct ttaccatctg gtcatgacgc tgaaagacgc caacggcaac gttctggaag
2221 tggtgccaca acgcgttggc ttccgtgata tcaaagtgcg cgacggtctg ttctggatca
2281 ataaccgtta tgtgatgctg cacggcgtca accgtcacga caacgatcat cgcaaaggcc
2341 gcgccgttgg aatggatcgc gtcgagaaag atctccagtt gatgaagcag cacaatatca
2401 actccgtgcg taccgctcac tacccgaacg atccgcgttt ttacgaactg tgtgatatct
2461 acggcctgtt tgtgatggcg gaaaccgacg tcgaatcgca cggctttgct aatgtcggcg
2521 atattagccg tattaccgac gatccgcagt gggaaaaagt ctacgtcgag cgcattgttc
2581 gccatatcca cgcgcagaaa aaccatccgt cgatcatcat ctggtcgctg ggcaatgaat
2641 ccggctatgg ctgtaacatc cgcgcgatgt accatgcggc gaaacggctg gatgacacgc
2701 gactggtgca ttacgaagaa gatcgcgatg ctgaagtggt cgatattatt tccaccatgt
2761 acacccgcgt gccgctgatg aatgagtttg gtgaataccc gcatccgaag ccgcgcatca
2821 tctgtgaata tgctcatgcg atggggaacg gaccgggcgg gctgacggag taccagaacg
2881 tcttctataa gcacgattgc attcagggtc attatgtctg ggagtggtgc gaccacggga
2941 tccaggcaca ggacgaccac ggcaatgtct ggtataaatt cggcggcgac tacggcgact
3001 atcccaacaa ctataacttc tgtcttgatg gtttgatcta ttccgatcag acgccgggac
3061 cgggcctgaa agagtacaaa caggttatcg cgccggtaaa aatccacgcg cgggatctga
3121 ctcgcggcga gttgaaagtc gaaaataaac tgtggtttac cacgcttgat gactacaccc
3181 tgcacgcaga ggtgcgcgcc gaaggtgaaa gcctcgcgac gcagcagatt aaactgccgg
3241 acgttgcgcc gaacagcgaa gcccccttgc agatcacgct gccgcagctg gacgcccgcg
3301 aagcgttcct caacattacg gtgaccaaag attcccgcac ccgctacagc gaagccggac
3361 accctatcgc cacttatcag ttcccgctga aggaaaacac cgcgcagcca gtgcctttcg
3421 caccaaataa tgcgcgtccg ctgacgctgg aagacgatcg tttgagctgc accgttcgcg
3481 gctacaactt cgcgatcacc ttctcaaaaa tgagtggcaa accgacatcc tggcaggtga
3541 atggcgaatc gctgctgact cgcgagccaa agatcaactt cttcaagccg atgatgatcg
3601 acaaccacaa gcaggagtac gaagggctgt ggcaaccgaa tcatttgcag atcatgcagg
3661 aacatctgcg cgactttgcc gtagaacaga gcgatggtga agtgctgatc atcagccgca
3721 cagttattgc cccgccggtg tttgacttcg ggatgcgctg cacctacatc tggcgcatcg
3781 ctgccgatgg ccaggttaac gtggcgcttt ccggcgagcg ttacggcgac tatccgcaca
3841 tcattccgtg catcggtttc accatgggaa ttaacggcga atacgatcag gtggcgtatt
3901 acggtcgtgg accgggcgaa aactacgccg acagccagca ggctaacatc atcgatatct
3961 ggcgccaagc cgtcgatgcc atgttcgaga actatccctt cccgcagaac aacggtaacc
4021 gtcagcatgt ccgctggacg gcactgacta accgccacgg taacggtctg ctggtggttc
4081 cgcagcgccc aattaacttc agcgcctggc actataccca ggaaaacatc cacgctgccc
4141 agcactgtaa cgagctgcag cgcagtgatg acatcaccct gaacctcgat caccagctgc
4201 ttggcctcgg ctccaactcc tggggcagcg aggtgctgga ctcctggcgc gtctggttcc
4261 gtgacttcag ctacggcttt acgttgctgc cggtttctgg cggagaagct accgcgcaaa
4321 gcctggcgtc gtatgagttc ggcgcagggt tcttttccac gaatttgcac acggagaata
4381 agcaatgagg atcatcgata acttagaaca gttccgccag atttacgcct ctggcaagaa
4441 gtggcaacgc tgcgttgaag cgattgaaaa tatcgacaac attcagcctg gcgtcgccca
4501 ctccatcggt gactcattga cttaccgcgt ggagacagac tccgcgaccg atgcgctatt
4561 taccgggcat cgacgctatt ttgaagtgca ttactacctg caagggcagc aaaaaattga
4621 atatgcgccg aaagagacat tacaggtagt ggaatattat cgtgatgaaa ctgaccgtga
4681 atatttaaaa ggctgcggag aaaccgttga ggtccacgaa gggcaaatcg ttatttgcga
4741 tatccatgaa gcgtatcggt ttatctgcaa taacgcggtc aaaaaagtgg ttctcaaagt
4801 caccatcgaa gatgttattt ccataacaaa taacaactac ggcggcaaaa ggagtttgcc
4861 gccaccgcta ccctactcat tttcggagat gtgttatgtc tgataccaaa cgtaatacaa
4921 tcggcaaatt cggcttcgtc tcgctgactt ttgccgccgt ttacagcttt aacaacgtta
4981 tga
//GenBank-Updates@genbank.bio.net (05/03/91)
LOCUS ECOEBGRA 4983 bp ds-DNA BCT 03-MAY-1991
DEFINITION E.coli ebgR, ebgA, ebgC genes complete cds.
ACCESSION M64441 M13700 M13796
KEYWORDS EBG enzyme alpha subunit; EBG enzyme beta subunit; EGB repressor;
ebgA gene; ebgC gene; ebgR gene.
SOURCE E.coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Stokes,H.W., Betts,P.W. and Hall,B.G.
TITLE Sequence of the ebgA gene of Escherichia coli: Comparison with the
lacZ gene
JOURNAL Mol. Biol. Evol. 2, 469-477 (1985)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Stokes,H.W. and Hall,B.G.
TITLE Sequence of the ebgR gene of Escherichia coli: Evidence that the
EBG and LAC operons are descended from a common ancestor
JOURNAL Mol. Biol. Evol. 2, 478-483 (1985)
STANDARD full staff_review
REFERENCE 3 (bases 1 to 4983)
AUTHORS Hall,B.G., Betts,P.W. and Wootton,J.C.
TITLE DNA sequence analysis of artificially evolved ebg enzyme and ebg
repressor genes
JOURNAL Genetics 123, 635-648 (1989)
STANDARD full automatic
COMMENT The sequence presented in Genetics 123, 635-648 (1989) is the
corrected sequence for those which appeared in Mol. Biol. Evol. 2,
469-477 (1985) and in Mol. Biol. Evol. 2, 478-483 (1985) and which
had accession numbers M13700 and M13796.
FEATURES Location/Qualifiers
CDS 126..1109
/product="EBG repressor"
/gene="ebgR"
/codon_start=126
mutation 695..695
/note="'ebgR, results in increased sensitivity to
inducers'"
/note="'G' in mutation"
mutation 708..708
/note="'ebgR, results in increased sensitivity to
inducers'"
/note="'A' in mutation"
mutation 712..712
/note="'ebgR, results in increased sensitivity to
inducers'"
/note="'G' in mutation"
mutation 760..761
/note="'ebgR-, ochre, constitutive synthesis of EBG
enzyme'"
/note="'AA' in mutation"
mutation 1021..1021
/note="'ebgR-, singe base insertion causes frameshift,
constitutive'"
/note="'TT' in mutation"
CDS 1293..4388
/product="EBG enzyme alpha subunit"
/gene="ebgA"
/codon_start=1293
mutation 1566..1566
/note="'ClassI mutation site in ebgA'"
/note="'A' in mutation"
mutation 4223..4223
/note="'Class II site in ebgA'"
/note="'T' in mutation"
mutation 4227..4227
/note="'Class V site in ebgA'"
/note="'G' in mutation"
CDS 4385..4903
/product="EGB enzyme beta subunit"
/gene="ebgC"
/codon_start=4385
mutation 4749..4749
/note="'ebgC mutation present in some Class IV alleles'"
/note="'G' in mutation"
BASE COUNT 1245 a 1338 c 1269 g 1131 t
ORIGIN
1 ttcgtgagcc gctgtgttac cgttacagcg tcaaagaaac gcgctttatt tactgaaaac
61 aggtgacccg ataagcactt cctctacaat gggggcgcac atcagggaaa gtaaaaaagg
121 taaacatggc aacactaaaa gacatcgcaa tcgaagctgg cgtatccctg gcgacagtat
181 ccagggtctt aaatgacgat ccgacattga atgtgaaaga agagacgaaa catcgcattc
241 tcgagatcgc cgaaaagctg gagtacaaga ccagtagtgc ccgtaaactc cagacaggtg
301 cagtcaacca acaccatatt ctggctatct acagctacca gcaggagctg gagatcaacg
361 atccttacta tctggcgatc cgccacggca ttgaaaccca gtgcgaaaag ctgggcatcg
421 agctcaccaa ctgttatgaa cacagcggct taccagacat taaaaacgtc accggtattt
481 taattgtcgg caaacccacg cccgccctgc gcgccgctgc cagcgcgttg accgacaata
541 tctgttttat cgactttcac gaacccggca gcggttacga tgcggtggat atcgatctgg
601 cacgcatcag taaagaaatc atcgacttct atatcaacca gggcgttaat cgtattggtt
661 ttattggcgg tgaagatgag cctggcaagg cggatattcg tgaggtcgcc tttgcggaat
721 atggccgact gaaacaagtg gtacgcgaag aggatatctg gcgcggcggt ttttccagtt
781 cgtcgggtta tgaactggca aaacaaatgc tggcgcggga agactatccg aaggcactgt
841 ttgttgcttc cgattccatt gctatcggcg tactgcgggc aattcatgaa cgaggcctga
901 acatcccaca ggatatttcg cttatcagcg ttaacgatat ccccaccgcg cgatttacct
961 ttccgccgct ctccaccgtg cgcatccatt ccgagatgat gggaagtcag ggcgttaacc
1021 tggtgtatga aaaagcccgc gatggtcgcg cgctgccgct gttagtcttc gttcccagca
1081 aattaaaact gcgcggcacg acccgttaaa tccccttaca cactgtccgg caatcgtttt
1141 tgccggacag tgctgccgtt tattttcgtg atccagttaa agtaaatgca tttacctgct
1201 actttttagt aaaaatttta ctaaactccc cagcaattac acaaactacc atcaccatga
1261 atggttccga tttctctcta ccgggaggcc ctatgaatcg ctgggaaaac attcagctca
1321 cccacgaaaa ccgacttgcg ccgcgtgcgt actttttttc atatgattct gttgcgcaag
1381 cgcgtacctt tgcccgcgaa accagcagcc tgtttctgcc cttaagcggt cagtggaatt
1441 tccacttttt tgaccatccg ctgcaagtac cagaagcctt cacctctgag ttaatggctg
1501 actgggggca tattaccgtc cccgccatgt ggcaaatgga aggtcacggc aaactgcaat
1561 ataccgacga aggttttccg ttccccatcg atgtgccgtt tgtccccagc gataacccaa
1621 ccggtgccta tcaacgtatt ttcaccctca gcgacggctg gcagggtaaa cagacgctga
1681 ttaaatttga cggcgtcgaa acctattttg aagtctatgt taacggtcag tatgtgggtt
1741 tcagcaaggg cagtcgcctg accgcagagt ttgacatcag cgcgatggtt aaaaccggcg
1801 acaacctgtt gtgtgtgcgc gtgatgcagt gggcggactc tacctacgtg gaagaccagg
1861 atatgtggtg gtcagcgggg atcttccgcg atgtttatct ggtcggaaaa cacctaacgc
1921 atattaacga tttcactgtg cgtaccgact ttgacgaagc ctattgcgat gccacgcttt
1981 cctgcgaagt ggtgctggaa aatctcgccg cctcccctgt cgtcacgacg ctggaatata
2041 ccctgtttga tggcgaacgc gtggtgcaca gcagcgccat tgatcatttg gcaattgaaa
2101 aactgaccag cgccacgttt gcttttactg tcgaacagcc gcagcaatgg tcagcagaat
2161 ccccttatct ttaccatctg gtcatgacgc tgaaagacgc caacggcaac gttctggaag
2221 tggtgccaca acgcgttggc ttccgtgata tcaaagtgcg cgacggtctg ttctggatca
2281 ataaccgtta tgtgatgctg cacggcgtca accgtcacga caacgatcat cgcaaaggcc
2341 gcgccgttgg aatggatcgc gtcgagaaag atctccagtt gatgaagcag cacaatatca
2401 actccgtgcg taccgctcac tacccgaacg atccgcgttt ttacgaactg tgtgatatct
2461 acggcctgtt tgtgatggcg gaaaccgacg tcgaatcgca cggctttgct aatgtcggcg
2521 atattagccg tattaccgac gatccgcagt gggaaaaagt ctacgtcgag cgcattgttc
2581 gccatatcca cgcgcagaaa aaccatccgt cgatcatcat ctggtcgctg ggcaatgaat
2641 ccggctatgg ctgtaacatc cgcgcgatgt accatgcggc gaaacggctg gatgacacgc
2701 gactggtgca ttacgaagaa gatcgcgatg ctgaagtggt cgatattatt tccaccatgt
2761 acacccgcgt gccgctgatg aatgagtttg gtgaataccc gcatccgaag ccgcgcatca
2821 tctgtgaata tgctcatgcg atggggaacg gaccgggcgg gctgacggag taccagaacg
2881 tcttctataa gcacgattgc attcagggtc attatgtctg ggagtggtgc gaccacggga
2941 tccaggcaca ggacgaccac ggcaatgtct ggtataaatt cggcggcgac tacggcgact
3001 atcccaacaa ctataacttc tgtcttgatg gtttgatcta ttccgatcag acgccgggac
3061 cgggcctgaa agagtacaaa caggttatcg cgccggtaaa aatccacgcg cgggatctga
3121 ctcgcggcga gttgaaagtc gaaaataaac tgtggtttac cacgcttgat gactacaccc
3181 tgcacgcaga ggtgcgcgcc gaaggtgaaa gcctcgcgac gcagcagatt aaactgccgg
3241 acgttgcgcc gaacagcgaa gcccccttgc agatcacgct gccgcagctg gacgcccgcg
3301 aagcgttcct caacattacg gtgaccaaag attcccgcac ccgctacagc gaagccggac
3361 accctatcgc cacttatcag ttcccgctga aggaaaacac cgcgcagcca gtgcctttcg
3421 caccaaataa tgcgcgtccg ctgacgctgg aagacgatcg tttgagctgc accgttcgcg
3481 gctacaactt cgcgatcacc ttctcaaaaa tgagtggcaa accgacatcc tggcaggtga
3541 atggcgaatc gctgctgact cgcgagccaa agatcaactt cttcaagccg atgatgatcg
3601 acaaccacaa gcaggagtac gaagggctgt ggcaaccgaa tcatttgcag atcatgcagg
3661 aacatctgcg cgactttgcc gtagaacaga gcgatggtga agtgctgatc atcagccgca
3721 cagttattgc cccgccggtg tttgacttcg ggatgcgctg cacctacatc tggcgcatcg
3781 ctgccgatgg ccaggttaac gtggcgcttt ccggcgagcg ttacggcgac tatccgcaca
3841 tcattccgtg catcggtttc accatgggaa ttaacggcga atacgatcag gtggcgtatt
3901 acggtcgtgg accgggcgaa aactacgccg acagccagca ggctaacatc atcgatatct
3961 ggcgccaagc cgtcgatgcc atgttcgaga actatccctt cccgcagaac aacggtaacc
4021 gtcagcatgt ccgctggacg gcactgacta accgccacgg taacggtctg ctggtggttc
4081 cgcagcgccc aattaacttc agcgcctggc actataccca ggaaaacatc cacgctgccc
4141 agcactgtaa cgagctgcag cgcagtgatg acatcaccct gaacctcgat caccagctgc
4201 ttggcctcgg ctccaactcc tggggcagcg aggtgctgga ctcctggcgc gtctggttcc
4261 gtgacttcag ctacggcttt acgttgctgc cggtttctgg cggagaagct accgcgcaaa
4321 gcctggcgtc gtatgagttc ggcgcagggt tcttttccac gaatttgcac acggagaata
4381 agcaatgagg atcatcgata acttagaaca gttccgccag atttacgcct ctggcaagaa
4441 gtggcaacgc tgcgttgaag cgattgaaaa tatcgacaac attcagcctg gcgtcgccca
4501 ctccatcggt gactcattga cttaccgcgt ggagacagac tccgcgaccg atgcgctatt
4561 taccgggcat cgacgctatt ttgaagtgca ttactacctg caagggcagc aaaaaattga
4621 atatgcgccg aaagagacat tacaggtagt ggaatattat cgtgatgaaa ctgaccgtga
4681 atatttaaaa ggctgcggag aaaccgttga ggtccacgaa gggcaaatcg ttatttgcga
4741 tatccatgaa gcgtatcggt ttatctgcaa taacgcggtc aaaaaagtgg ttctcaaagt
4801 caccatcgaa gatgttattt ccataacaaa taacaactac ggcggcaaaa ggagtttgcc
4861 gccaccgcta ccctactcat tttcggagat gtgttatgtc tgataccaaa cgtaatacaa
4921 tcggcaaatt cggcttcgtc tcgctgactt ttgccgccgt ttacagcttt aacaacgtta
4981 tga
//