GenBank-Updates@genbank.bio.net (04/27/91)
LOCUS R10CMLA 2141 bp ds-DNA BCT 27-APR-1991
DEFINITION Plasmid R1033 from Pseudomonas aeruginosa cmlA gene, complete cds,
and streptomycin/spectinomycin 3''-adenyltransferase gene, 3' end.
ACCESSION M64556
KEYWORDS streptomycin-spectinomycin resistance gene;
streptomycin/spectinomycin adenyltransferase.
SOURCE Plasmid R1033 from Pseudomonas aeruginosa DNA.
ORGANISM Plasmid R1033
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 2141)
AUTHORS Roy,P.H., Buisson,J.-P., Champetier,S. and Bissonnette,L.
TITLE Characterization of the nonenzymatic chloramphenicol resistance
(cmlA) gene of the In4 integron of Tn1696: Similarity of the
product to transmembrane transport proteins
JOURNAL Unpublished (1991)
STANDARD full automatic
FEATURES Location/Qualifiers
CDS <1..339
/product="'streptomycin/spectinomycin
3''-adenyltransferase'"
/gene="aadA2"
/codon_start=1
misc_recomb 394^396
/organism="'Pseudomonas aeruginosa, beginning
ofintegrase-mediated cmlA cassette insertion'"
-35_signal 416..421
/note="'p1, probable promoter'"
-35_signal 428..433
/note="'p2, possible alternate promoter'"
-35_signal 435..440
/note="'p3, possible alternate promoter'"
-10_signal 439..444
/note="'p1'"
-10_signal 450..455
/note="'p2'"
-10_signal 458..463
/note="'p3'"
RBS 588..592
CDS 601..1860
/note="'GTG initiation codon'"
/product="'chloramphenicol resistance protein'"
/gene="cmlA"
/codon_start=601
misc_recomb 1943^1945
/organism="'Pseudomonas aeruginosa, end of cmlA cassette
insertion'"
CDS 2052..>2141
/product="'putative ethidium bromide-resistanceprotein'"
/gene="ORF4"
/codon_start=2052
BASE COUNT 447 a 529 c 596 g 569 t
ORIGIN
1 gaattctttg acccggttcc tgaacaggat ctattcgagg cgctgaggga aaccttgaag
61 ctatggaact cgcagcccga ctgggccggc gatgagcgaa atgtagtgct tacgttgtcc
121 cgcatttggt acagcgcaat aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
181 gcaataaaac gcctacctgc ccagtatcag cccgtcttac ttgaagctaa gcaagcttat
241 ctgggacaaa aagaagatca cttggcctca cgcgcagatc acttggaaga atttattcgc
301 tttgtgaaag gcgagatcat caagtcagtt ggtaaatgat gtctaacaat tcgttcaagc
361 cgaccgcgct acgcgcggcg gcttaactcc ggcgttgggc gcacaataag gctccttgca
421 gagttgcttg aaagttgtta cgattcaaat tcaatcatga gatagtcagc agatgagcac
481 ttccaagaac gcagacaagt aagccgcagc aaccttcatt tttcggttgt tgcggcgttc
541 tcatgaatcc ttttgctcta cgggagcgcc gccaaatcct ttgttcaagg agatggtttc
601 gtgagctcaa aaaactttag ttggcggtac tcccttgccg ccacggtgtt gttgttatca
661 ccgttcgatt tattggcatc actcggcatg gacatgtact tgccagcagt gccgtttatg
721 ccaaacgcgc ttggtacgac agcgagcaca attcagctta cgctgacaac gtacttggtc
781 atgattggtg ccggtcagct cttgtttgga ccgctatcgg accgactggg gcgccgcccc
841 gttctactgg gaggtggcct cgcctacgtt gtggcgtcaa tgggcctcgc tcttacgtca
901 tcggctgaag tctttctggg gcttcggatt cttcaggctt gtggtgcctc ggcgtgcctt
961 gtttccacat ttgcaacagt acgtgacatt tacgcaggtc gcgaggaaag taatgtcatt
1021 tacggcatac tcggatccat gctggccatg gtcccggcgg taggcccatt gctcggagcg
1081 ctcgtcgaca tgtggcttgg gtggcgggct atctttgcgt ttctaggttt gggcatgatc
1141 gctgcatctg cagcagcgtg gcgattctgg cctgaaaccc gggtgcaacg agttgcgggc
1201 ttgcaatggt cgcagctgct actccccgtt aagtgcctga acttctggtt gtacacgttg
1261 tgttacgccg ctggaatggg tagcttcttc gtctttttct ccattgcgcc cggactaatg
1321 atgggcaggc aaggtgtgtc tcagcttggc ttcagcctgc tgttcgccac agtggcaatt
1381 gccatggtgt ttacggctcg ttttatgggg cgtgtgatac ccaagtgggg cagcccaagt
1441 gtcttgcgaa tgggaatggg atgcctgata gctggagcag tattgcttgc catcaccgaa
1501 atatgggctt tgcagtccgt gttaggcttt attgctccaa tgtggctagt gggtattggt
1561 gtcgccacag cggtatctgt ggcgcccaat ggcgctcttc gaggattcga ccatgttgct
1621 ggaacggtca cggcagtcta cttctgcttg ggcggtgtac tgctaggaag catcggaacg
1681 ttgatcattt cgctgttgcc gcgcaacacg gcttggccgg ttgtcgtgta ctgtttgacc
1741 cttgcaacag tcgtgctcgg tctgtcttgt gtttcccgag tgaagggctc tcgcggccag
1801 ggggagcatg atgtggtcgc gctacaaagt gcgggaagta catcaaatcc caatcgttga
1861 gagaatgtgg caagctatcg cccaacaaat cgctgcagcc gacccaaaac cgctacgcgg
1921 tttcggtcgg ctgagctcag gcgttagatg cactaagcac ataattgctc acagccaaac
1981 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
2041 ggagatatat catgaaaggc tggctttttc ttgttatcgc aatagttggc gaagtaatcg
2101 caacatccgc attaaaatct agcgagggct ttactaagct t
//GenBank-Updates@genbank.bio.net (05/03/91)
LOCUS R10CMLA 2141 bp ds-DNA BCT 03-MAY-1991
DEFINITION Plasmid R1033 from Pseudomonas aeruginosa cmlA gene, complete cds,
and streptomycin/spectinomycin 3''-adenyltransferase gene, 3' end.
ACCESSION M64556
KEYWORDS streptomycin-spectinomycin resistance gene;
streptomycin/spectinomycin adenyltransferase.
SOURCE Plasmid R1033 from Pseudomonas aeruginosa DNA.
ORGANISM Plasmid R1033
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 2141)
AUTHORS Bissonnette,L., Champetier,S., Buisson,J.-P. and Roy,P.H.
TITLE Characterization of the nonenzymatic chloramphenicol resistance
(cmlA) gene of the In4 integron of Tn1696: Similarity of the
product to transmembrane transport proteins
JOURNAL Unpublished (1991)
STANDARD full automatic
FEATURES Location/Qualifiers
CDS <1..339
/product="'streptomycin/spectinomycin
3''-adenyltransferase'"
/gene="aadA2"
/codon_start=1
misc_recomb 394^396
/organism="'Pseudomonas aeruginosa, beginning of
integrase-mediated cmlA cassette insertion'"
RBS 588..592
CDS 601..1860
/note="'GTG initiation codon'"
/product="'chloramphenicol resistance protein'"
/gene="cmlA"
/codon_start=601
misc_recomb 1943^1945
/organism="'Pseudomonas aeruginosa, end of cmlA cassette
insertion'"
CDS 2052..>2141
/product="'putative ethidium bromide-resistance protein'"
/gene="ORF4"
/codon_start=2052
BASE COUNT 447 a 529 c 596 g 569 t
ORIGIN
1 gaattctttg acccggttcc tgaacaggat ctattcgagg cgctgaggga aaccttgaag
61 ctatggaact cgcagcccga ctgggccggc gatgagcgaa atgtagtgct tacgttgtcc
121 cgcatttggt acagcgcaat aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
181 gcaataaaac gcctacctgc ccagtatcag cccgtcttac ttgaagctaa gcaagcttat
241 ctgggacaaa aagaagatca cttggcctca cgcgcagatc acttggaaga atttattcgc
301 tttgtgaaag gcgagatcat caagtcagtt ggtaaatgat gtctaacaat tcgttcaagc
361 cgaccgcgct acgcgcggcg gcttaactcc ggcgttgggc gcacaataag gctccttgca
421 gagttgcttg aaagttgtta cgattcaaat tcaatcatga gatagtcagc agatgagcac
481 ttccaagaac gcagacaagt aagccgcagc aaccttcatt tttcggttgt tgcggcgttc
541 tcatgaatcc ttttgctcta cgggagcgcc gccaaatcct ttgttcaagg agatggtttc
601 gtgagctcaa aaaactttag ttggcggtac tcccttgccg ccacggtgtt gttgttatca
661 ccgttcgatt tattggcatc actcggcatg gacatgtact tgccagcagt gccgtttatg
721 ccaaacgcgc ttggtacgac agcgagcaca attcagctta cgctgacaac gtacttggtc
781 atgattggtg ccggtcagct cttgtttgga ccgctatcgg accgactggg gcgccgcccc
841 gttctactgg gaggtggcct cgcctacgtt gtggcgtcaa tgggcctcgc tcttacgtca
901 tcggctgaag tctttctggg gcttcggatt cttcaggctt gtggtgcctc ggcgtgcctt
961 gtttccacat ttgcaacagt acgtgacatt tacgcaggtc gcgaggaaag taatgtcatt
1021 tacggcatac tcggatccat gctggccatg gtcccggcgg taggcccatt gctcggagcg
1081 ctcgtcgaca tgtggcttgg gtggcgggct atctttgcgt ttctaggttt gggcatgatc
1141 gctgcatctg cagcagcgtg gcgattctgg cctgaaaccc gggtgcaacg agttgcgggc
1201 ttgcaatggt cgcagctgct actccccgtt aagtgcctga acttctggtt gtacacgttg
1261 tgttacgccg ctggaatggg tagcttcttc gtctttttct ccattgcgcc cggactaatg
1321 atgggcaggc aaggtgtgtc tcagcttggc ttcagcctgc tgttcgccac agtggcaatt
1381 gccatggtgt ttacggctcg ttttatgggg cgtgtgatac ccaagtgggg cagcccaagt
1441 gtcttgcgaa tgggaatggg atgcctgata gctggagcag tattgcttgc catcaccgaa
1501 atatgggctt tgcagtccgt gttaggcttt attgctccaa tgtggctagt gggtattggt
1561 gtcgccacag cggtatctgt ggcgcccaat ggcgctcttc gaggattcga ccatgttgct
1621 ggaacggtca cggcagtcta cttctgcttg ggcggtgtac tgctaggaag catcggaacg
1681 ttgatcattt cgctgttgcc gcgcaacacg gcttggccgg ttgtcgtgta ctgtttgacc
1741 cttgcaacag tcgtgctcgg tctgtcttgt gtttcccgag tgaagggctc tcgcggccag
1801 ggggagcatg atgtggtcgc gctacaaagt gcgggaagta catcaaatcc caatcgttga
1861 gagaatgtgg caagctatcg cccaacaaat cgctgcagcc gacccaaaac cgctacgcgg
1921 tttcggtcgg ctgagctcag gcgttagatg cactaagcac ataattgctc acagccaaac
1981 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
2041 ggagatatat catgaaaggc tggctttttc ttgttatcgc aatagttggc gaagtaatcg
2101 caacatccgc attaaaatct agcgagggct ttactaagct t
//