[bionet.molbio.genbank.updates] Bacteriophage Mu wild type DNA fragment with a gyrase cleavage

GenBank-Updates@genbank.bio.net (03/27/91)

LOCUS       PMUCEN        150 bp ds-DNA             PHG       27-MAR-1991
DEFINITION  Bacteriophage Mu wild type DNA fragment with a gyrase cleavage
            site.
ACCESSION   M32302
KEYWORDS    .
SOURCE      Bacteriophage Mu (wild type) DNA.
  ORGANISM  Bacteriophage mu
            Viridae; ds-DNA nonenveloped viruses; Myoviridae.
REFERENCE   1  (bases 1 to 150)
  AUTHORS   Pato,M.L., Howe,M.M. and Higgins,N.P.
  TITLE     A DNA gyrase-binding site at the center of the bacteriphage Mu
            genome is required for efficient replicative transposition
  JOURNAL   Unpublished (1990) In Press
  STANDARD  full staff_review
COMMENT     Draft entry and computer-readable sequence for [Unpublished (1990)
            In Press] kindly submitted
            by M.L.Pato, 23-FEB-1990.
FEATURES             Location/Qualifiers
     mutation        44..44
                     /note="a in wt; g in Mu nuB103"
     misc_feature    49..50
                     /note="DNA gyrase cleavage site in complimentary strand"
     mutation        50..50
                     /note="g in wt; c in Mu nuB1"
BASE COUNT       40 a     38 c     27 g     45 t
ORIGIN      Map position at 18.0 kb.
        1 acgcgtcagc gccgctctga ggcaataaac agaatcaggc ataaaatcag ccgcacagat
       61 tttttaaaac gcgccacggg atttttaaac cggtatttaa cggtgtatga atcccgtttt
      121 atcttccttt cactttcttt ctccagtact
//

GenBank-Updates@genbank.bio.net (05/04/91)

LOCUS       PMUCEN        150 bp ds-DNA             PHG       04-MAY-1991
DEFINITION  Bacteriophage Mu wild type DNA fragment with a gyrase cleavage
            site.
ACCESSION   M32302
KEYWORDS    .
SOURCE      Bacteriophage Mu (wild type) DNA.
  ORGANISM  Bacteriophage mu
            Viridae; ds-DNA nonenveloped viruses; Myoviridae.
REFERENCE   1  (bases 1 to 150)
  AUTHORS   Pato,M.L., Howe,M.M. and Higgins,N.P.
  TITLE     A DNA gyrase-binding site at the center of the bacteriphage Mu
            genome is required for efficient replicative transposition
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 87, 8716-8720 (1990)
  STANDARD  full staff_review
COMMENT     Draft entry and computer-readable sequence for [Unpublished (1990)
            In Press] kindly submitted
            by M.L.Pato, 23-FEB-1990.
FEATURES             Location/Qualifiers
     mutation        44..44
                     /note="a in wt; g in Mu nuB103"
     misc_feature    49..50
                     /note="DNA gyrase cleavage site in complimentary strand"
     mutation        50..50
                     /note="g in wt; c in Mu nuB1"
BASE COUNT       40 a     38 c     27 g     45 t
ORIGIN      Map position at 18.0 kb.
        1 acgcgtcagc gccgctctga ggcaataaac agaatcaggc ataaaatcag ccgcacagat
       61 tttttaaaac gcgccacggg atttttaaac cggtatttaa cggtgtatga atcccgtttt
      121 atcttccttt cactttcttt ctccagtact
//