GenBank-Updates@genbank.bio.net (05/08/91)
LOCUS TOBCPLB3 1268 bp ds-DNA ORG 08-MAY-1991
DEFINITION Tobacco Leu-tRNA and Phe-tRNA genes.
ACCESSION M16898
KEYWORDS transfer RNA-Leu; transfer RNA-Phe.
SEGMENT 3 of 3
SOURCE Tobacco (variety Bright Yellow 4) chloroplast DNA.
ORGANISM Chloroplast Nicotiana tabacum
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Asteridae; Solanales; Solanaceae.
REFERENCE 1 (bases 1 to 1268)
AUTHORS Yamada,K., Shinozaki,K. and Sugiura,M.
TITLE DNA sequences of tobacco chloroplast genes for tRNA-Ser (GGA),
tRNA-Thr (UGU), tRNA-Leu (UAA), tRNA-Phe (GAA): the tRNA-Leu gene
contains a 503 by intron
JOURNAL Plant Mol. Biol. 6, 193-199 (1986)
STANDARD simple staff_review
COMMENT The Leu-tRNA anticodon (TAA) begins with the last bp (119) of exon
1, and ends with the first two bp (623-624) of exon 2.
FEATURES Location/Qualifiers
tRNA join(85..119,623..673)
/note="Leu-tRNA"
tRNA 85..119
/note="Leu-tRNA, exon 1"
intron 120..622
/note="Leu-tRNA, intron A (no splice consensus)"
tRNA 623..673
/note="Leu-tRNA, exon 2"
tRNA 1029..1101
/anticodon=(pos:1062..1064,aa:Phe)
/note="Phe-tRNA"
BASE COUNT 443 a 223 c 226 g 376 t
ORIGIN About 0.7 kb after segment 2.
1 taatgaattc aatggttcca gtataaatga aagaaaaaga aaaaggaatg acatcacaac
61 gagatcctaa tctcaaaaag aaagggggat atggcgaaat cggtagacgc tacggactta
121 attggattga gccttggtat ggaaacttac taagtgatca ctttcaaatt cagagaaacc
181 ctggaattaa caaaaatggg caatcctgag ccaaatcctg ttttccgaaa acaaacaaag
241 gttcagaaaa aaaggatagg tgcagagact caatggaagc tattctaaca aatggagtta
301 aatgcgttgg tagaggaatc tttacatcga aacttcagaa agaaaaagaa tgaagtgaag
361 gataaacgta tatacatacg tattgaatac tatatcaaaa tcaaatgatt aatgatgacc
421 cgaatctgta ttttttctat aaaaaataga agaattggtg tgaatcgatt ctacattgaa
481 gaaagaatcg aatattcatt gatcaaacca ttcactccat agtctgatag atcttttgaa
541 gaactgatta atcggacgag aataaagata gagtcccgtt ctacatgtca ataccggcaa
601 caatgaaatt tatcgtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
661 cctctatccc caaaaagact atttcactcc ccaactattt atccgacccc ctttccttag
721 cggttccaaa ttccttatct ttctcattca ctctattctt ttagaaatgg atttgagcgt
781 aaatggcttt ctcttatcac aagtcttgtg atatatatga tacacataga aatgaacgtc
841 tttgagcaag gaatccctag ttgaatgatt ccctatcaat atcattactc atactgaaac
901 ttacaaagtc atctttttga agatcgaaga aattccccgg ctttgagaaa atttttaatc
961 tacttttgtc cttgtaattg acatagaccc cagttctcta ataaaatgag gatactacat
1021 tgggaatagc cgggatagct cagttggtag agcagaggac tgaaaatcct cgtgtcacca
1081 gttcaaatct ggttcctggc acatgattaa tttgtatggg tctctcttcc ctcgaattaa
1141 tttctaatta attgatatga atcaacatac atattctttt agagtctaga ttagaataat
1201 agctttatcc agtttggcga gatatacccc atctatgttc tagatgggta gagtttctta
1261 gataaagt
//