GenBank-Updates@genbank.bio.net (05/08/91)
LOCUS HUMAPOBA1 840 bp ds-DNA PRI 08-MAY-1991
DEFINITION Human apolipoprotein B-100 gene, exons 1 and 2.
ACCESSION M15053
KEYWORDS apolipoprotein B-100.
SEGMENT 1 of 2
SOURCE Human (adult) liver, cDNA to mRNA (library of E.F.Fritsch) clones
lambda-B[6,19,23]; DNA (library of T.Maniatis), clone lambda-GB18.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 840)
AUTHORS Cladaras,C., Hadzopoulou-Cladaras,M., Nolte,R.T., Atkinson,D. and
Zannis,V.I.
TITLE The complete sequence and structural analysis of human
apolipoprotein B-100: Relationship between apoB-100 and apoB-48
forms
JOURNAL EMBO J. 5, 3495-3507 (1986)
STANDARD full staff_review
COMMENT Computer-readable sequence for [EMBO J. 5, 3495-3507 (1986)] kindly
provided by C.Cladaras,
12-MAR-1987. Apolipoprotein B-100 is produced by liver and
intestine [2]. Two forms of apoB mRNA exist, about 8 and 15 kb
long. These two messages may have resulted from alternative
splicing, since only one gene was found in the genomic DNA.
FEATURES Location/Qualifiers
prim_transcript 155..>840
/note="apoB100 mRNA"
exon 283..355
/note="apolipoprotein B-100 precursor, exon 1
/nomgen='APOB' /map='2p24-p23' /hgml_locus_uid='LD0091N'"
sig_peptide 283..354
/note="apolipoprotein B-100 signal peptide"
/codon_start=283
mat_peptide 355..355
/note="apolipoprotein B-100"
intron 356..669
/note="apoB100 intron A"
exon 670..708
/note="apolipoprotein B-100 precursor, exon 2"
mat_peptide 670..708
/note="apolipoprotein B-100"
intron 709..>840
/note="apoB100 intron B"
BASE COUNT 132 a 266 c 312 g 130 t
ORIGIN 70 bp upstream of HpaI site; chromosome 2p24-p23.
1 cgtgccccct ccccgaggct cttcaaggct caaagagaag ccagtgtaga aaagcaaaca
61 ggtcaggccc gggaggcgcc ctttggacct tttgcaatcc tggcgctctt gcagcctggg
121 cttcctataa atggggtgcg ggcgccggcc gcgcattccc accgggacct gcggggctga
181 gtgcccttct cggttgctgc cgctgaggag cccgcccagc cagccagggc cgcgaggccg
241 aggccaggcc gcagcccagg agccgcccca ccgcagctgg cgatggaccc gccgaggccc
301 gcgctgctgg cgctgcctgc gctgctgctg ctgctgctgg cgggcgccag ggccggtgag
361 tgcgcggccg ctctgcgggc agcagaggga gcgggaggga gccggcggac cgaggttggc
421 cggggcagcc tgggcctagg ccagagggag ggcagccaca gggtccaggg cgagtggggg
481 gattggacca gctggcggcc cctgcaggct caggatgggg ggcgcgggat ggaggggctg
541 aggagggggt ctccggagcc tgcctccctc ctgaaaggtg aaacctgtgc cggtggtccc
601 cctgtcgggc ccctagcacc cgctgggaag acgtgggaag ctcacagatt tctttctcct
661 gtcttacaga agaggaaatg ctggaaaatg tcagcctggt ctgtccaagt aaggcatctg
721 cgcatggggc gtggaagggc gcccagcccc gtgcactctc ctacacccgg gtccctgagg
781 gcctcccact ctacagggct gagatggcat cgtggtgtgc cttgctctga cccaggaagc
//