GenBank-Updates@genbank.bio.net (05/08/91)
LOCUS HUMASA1PS 1877 bp ds-DNA PRI 08-MAY-1991
DEFINITION Human argininosuccinate synthetase pseudogene 1.
ACCESSION K01845
KEYWORDS argininosuccinate synthetase; processed gene; pseudogene;
synthetase.
SOURCE Human genomic DNA, Charon-4A library of Lawn et al, clone psi-AS-1.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 1877)
AUTHORS Freytag,S.O., Bock,H.-G.O., Beaudet,A.L. and O'Brien,W.E.
TITLE Molecular structures of human argininosuccinate synthetase
pseudogenes: Evolutionary and mechanistic implications
JOURNAL J. Biol. Chem. 259, 3160-3166 (1984)
STANDARD full staff_review
COMMENT There is one expressed gene and 14 pseudogenes fo argininosuccinate
synthetase in the human genome. Three of the pseudogenes (psi-AS-1,
-3, -7) were sequenced, in addition to the cDNA (AS). The
pseudogenes are 89-93% homologous to the cDNA and lack introns. The
authors [J. Biol. Chem. 259, 3160-3166 (1984)] estimate that
psi-AS-1 and psi-AS-3 were created 10-11
million years ago (93% homology), and psi-AS-7 was created
approximately 21 million years ago (89% homology).
The functional gene contains 13 exons spanning 65 kb on chromosome
9 (9q34-qter locus).
BASE COUNT 480 a 498 c 498 g 401 t
ORIGIN 927 bp upstream of AvaI site.
1 ttcaagacct tgtcaacttt aaggaggtta aacagaggaa aatcttggga cttttggcag
61 gattgcctct gaaaggaagt tggcaggagc taagtggctg aggcctaggg gaattaatta
121 ttaactcaag tgccctccag tggagaggag gctgagaaga ggccactctg ccatgctcca
181 caggccctag accactcccg agtggttcac tgcaccctga agacagattc cagacgctgg
241 gaactcgcgc ctccaatccc agacactatg tccagcaaag gctccatgag agttccggcc
301 tatggtggcg gcctagacac ctcctgcatc ctcgtgtggg tgaaggaaca aggctatgat
361 gtcattgcct atctggccaa cactggccag aaggaagact tcgaggaagc caggaagaag
421 gcactgaaac ttggggccaa aaaggtgttc attgaggatg tcatcaggga gtttgtggag
481 gagttcaact ggccggccat ccagtccagc acactgtatg aggaccgcta cctcctgggc
541 acctctctcg ccaggccctg cattgccggc aaacaagtgg aaatcgccca gcaggagggg
601 gtgtcccgcg gcgccaccgg taaggggaat atcaggtccg gtttgagctc acctggtact
661 cgctggtccc ccagataaag ggcactgctc cctggaggat gcccgagttc tacaaccggt
721 tcaagggccg ccctgacctg atggaatacg caaagcagca cgggattcct atcccggtca
781 ctcccaagaa cccgtggaac atggacgaga acctcatgca catcagctac gaggctggaa
841 tcatggagaa ccccaagaac caagtgcctc taggtctcta cacgaagacc taggaaccgg
901 tcaaagcccc caacacccct gacattctcg agatcgagtt aaaaaagggg tccccgtgaa
961 ggtgaccaac gtcaacgatg gctccaccca cccgacctcc ttggagttcc tgtacctgaa
1021 cgaagtcgcg gacaagcacg gtgtgggccg tactgacatc gtggagaacc gcttcactgg
1081 aatgaagtcc cgaggtatct atgagacccc agcaggcacc atcctttacc acactcattt
1141 agacatcaag gccttcacca tggactggga ggtgtgcaaa atcaaacaag gcctgggctt
1201 gaaatttgct gagctggtgt acaccgcttt ctggcatagc cctgagtgtg aatttgtccg
1261 ccactgcatc gccaagtccc aggagcaagt gggagggaaa gcgcaggtgt ccgtcctcaa
1321 gggccgggtg tacatccttg gctgggagtt cccactctct ccctacaacg aggagccggt
1381 gagcatgaac gtgtagggtg attatgagcc aattgaggcc accgggttca tcaacatcaa
1441 ttccctcagg ctgaaggaat aggaatatca tcgtctgcag agcaaggtca ctgccaaata
1501 gacccctgta caatgaggag ctgaggcctc ctcaatttgc agatccccca agtacaggcg
1561 ctaattgttg tgataatttg taattgtgac ttgttcttcc cggctggcag cgtagtgggg
1621 ctaccaggcc ccagctttgt tccctggtcc ccctgaagcc tgcaaacgtc gtcatctaag
1681 ggaagggtgg ggggcagctg cggtgggtag ctattaaatg acaattaaaa gatacactag
1741 taaaaaaaaa aattgactgc tctgaaaacg agtctactgg gcagtgacat tcacatttct
1801 gaaattaatg catactctat ttgcattttc tctttttcct gtcttcacct gactttgaca
1861 attagtaaat aaatccc
//