GenBank-Updates@genbank.bio.net (05/08/91)
LOCUS YSCTYA117 11220 bp ds-DNA PLN 08-MAY-1991 DEFINITION Yeast transposon Ty1-17 and flanking Glu-tRNA and Leu-tRNA genes, and beta-isopropylmalate dehydrogenase LEU2) gene, complete cds. ACCESSION X03840 M19542 X02991 KEYWORDS Ty1 transposon B10; beta-isopropylmalate dehydrogenase; transfer RNA-Glu; transfer RNA-Leu. SOURCE Yeast (S.cerevisiae) DNA. ORGANISM Saccharomyces cerevisiae Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes; Endomycetales; Saccharomycetaceae. REFERENCE 1 (bases 2885 to 9388) AUTHORS Warmington,J.R., Waring,R.B., Newlon,C.S., Indge,K.J. and Oliver,S.G. TITLE Nucleotide sequence characterization of Ty 1-17, a class II transposon from yeast JOURNAL Nucleic Acids Res. 13, 6679-6693 (1985) STANDARD simple automatic REFERENCE 2 (bases 1 to 3840) AUTHORS Warmington,J.R., Anwar,R., Newlon,C.S., Waring,R.B., Davies,R.W., Indge,K.J. and Oliver,S.G. TITLE A 'hot-spot' for Ty transposition on the left arm of yeast chromosome III JOURNAL Nucleic Acids Res. 14, 3475-3485 (1986) STANDARD simple staff_review REFERENCE 3 (bases 1 to 11220) AUTHORS Warmington,J.R. JOURNAL Unpublished (1987) Univ. of Manchester, Manchester M60 1QD UK STANDARD simple staff_review COMMENT EMBL features not translated to GenBank features: key from to description SITE 1575 1590 delta insert SITE 1627 1636 delta insert RPT 1818 1680 (C) repeat delta sequence RPT 1819 1878 repeat delta sequence RPT 2680 2685 direct repeat 1 RPT 2685 2910 repeat delta A sequence RPT 2906 2910 direct repeat 2 RPT 2911 3243 repeat delta B sequence RPT 3244 3378 repeat delta A sequence RPT 3244 3248 direct repeat 2 RPT 3378 3382 direct repeat 1 TPOSON 3427 9388 Ty 1-17 RPT 3427 3758 direct repeat, delta sequence PRM 3593 3599 put. TATA-box RBS 3667 3671 put. rRNA binding site SITE 3759 9056 epsilon sequence SITE 3759 3782 sequence homologous to yeast tRNA-Met INVREP 3819 3832 inverted repeat A SITE 4075 4092 put. regulatory region; sequence homologous to diploid control site OPR 4215 4229 enhancer-like element SITE 4241 4258 put. regulatory region; sequence homologous to diploid control site RPT 9057 9388 direct repeat, delta sequence INVREP 9170 9183 inverted repeat A' OPR 9309 9312 5' end of put. transcript termination signal SITE 9357 9362 transcription termination region FEATURES Location/Qualifiers tRNA 1428..1499 /note="Glu-tRNA" CDS 3718..5034 /note="TYA" /codon_start=3718 CDS 4991..9034 /note="TYB" /codon_start=4991 tRNA 9478..9594 /note="Leu-tRNA" CDS 9943..11037 /note="beta-isopropylmalate dehydrogenase (LEU 2)" /codon_start=9943 BASE COUNT 3998 a 2177 c 1933 g 3112 t ORIGIN 1 bp upstream of BamHI site; chromosome III. 1 ggatcccgga ttaacgcctg ttactgagac acttcctaac aacctaaaag aaaaaacagc 61 tctgctgcag gatactgaaa agaaaataat agaaacgata cgcagatcca aatttttagg 121 atcgctacta aatgttagag ggggactatc gccagggaaa agtgaactgg cacctatcga 181 agagtctccc atagtttcaa ccacaccact aatatataat gatcgaatgg aacctcgtag 241 gatatccgat gtggaagtcc cacatttcac gaggaaatca aaacacttta ctactgctaa 301 taatcggcgc tcagtcttat ctttgtatgc gaaggattca atcaaagact taaacgaatt 361 tttaataaag gaagatcctg atttgcctcc acaaggaagc actgataacg aaagtaggag 421 cgaagatccc gaaatagccg agagtatcac tgattcaagg aatatacaat atgatgagga 481 tgatagtaag gatggtgata atgtgaataa tgataatata ttgagcgact ttccctcaag 541 gcgttggcat atcacaggaa tacgacatga aggataaaaa tccaaaccaa tctccaatat 601 caaaaagcgc agagcccaca ctggtagtga aacttccgtc tttgagttct ttccaaggaa 661 aaaacgccag tgggttgggc ctataccaaa gagagccttc taaggtaacc ttaccgagcc 721 ttacaagtaa taacagcagc gtcggagaaa acactagagg atggggcgga aaaagggact 781 gagagtgaga aaatcgctgc ttccctgtca gacgatgact tgaaggaaga taatgacaag 841 aaagataacg acacagtgaa tgccctacta cagtaaaaaa gccacctaac agcgtactct 901 tgaaaaaatt ctcaaaaggt aaaatcttag aacttgaaat acatgccaaa atacctgaaa 961 agagactata cgagggccta cataaactac tagaaggctg gaagcaatac gggttgaaaa 1021 acctggtgtt taacatcact aatatgatca taacaggaaa attggtgaat gacagtattt 1081 tatttttacg atctacgctt tttgaaataa tggttttacc aaatggagac ggtagaagtt 1141 taattaaatt caataagaaa acaggatcta ctaaaacgct gaccaagctt gctacagaaa 1201 tccaaattat tttacaaaaa gaaggtgttt tggacaaata aaaaaaaggg agggataatc 1261 tatccccaaa tggacaatac gatgtaacac cgttatataa ttaataaatg tatatatgta 1321 actatttttt gaaaaaaata aaatggtaat ttattaaata tttggaccgg tcaacaatct 1381 cagatatagt tgaaaaaatc tttaattgta atagcagaaa aaaaaaactc cgatacgggg 1441 agtcgaaccc cggtctccac ggtgaaagcg tgatgtgata gccgttacac tatatcggat 1501 aacaaattat taaaattatt taataaaaag atgaactaat ggtgtataaa aacatcataa 1561 aaactcaact caactgttcc aataaaatct gcaatttaaa ttaattattc tgaaaaaaca 1621 cgtaaattgt tggaatggaa ccctaaaata tttcctcttt agtactattc atgttacttg 1681 agaaatgggt gaattttgag atagttgttg ggattccatt gttgataaag gctaataata 1741 ttaggtatac agaatatgct agaggttctc ctcgagcata taggaatcct caaaatggaa 1801 ttagcatttc tacataataa cctcaattcc cttttgtgga ttcacatatt ctcgatcgga 1861 actcctagca tagtgcagac aacactcaca tagctctccc atttatcaag ttggaattca 1921 ctccaagaaa ttttcttgct tctctctttc ccacagaaat gaaaaggaac agtttggcgg 1981 ctattgtttt agcccttact ttttggaccc tttgaaaaaa tgccaagaat gcgtgacggt 2041 ttccctccat gctcgagaac cacaaaaacc ctatgtggta ttcgattatg cgactcacgg 2101 caaatatttg acttgtcaaa gttattcggg atttccttga atccctgaga agtgaaatgg 2161 aaagaattta agggcataaa tacctttcaa gcacccagaa gaacagtaaa ataaagcaag 2221 gtacgtgaaa ttaatatttt taaatggttc taaccgatgc cgaagaactg cgcgatccgg 2281 ttataacgtc tgacatgtcc ttttttgatt tggaatccaa ccactcagtg actctgtcat 2341 tacttgcgaa attattccac aaattccatc gaaagtgaat cgcaaaccac cttcagactg 2401 gcaccgacaa agcaaagatt atacagacag agtactttat acgtaccgtt aagtctcaag 2461 caaagggttt tcttatttac tgaacgggta aagagtatct gggccggctt gccaagatgc 2521 aaaccgaata agtatttcaa agttgcattt gccttagccg tcctgacacc attggctatt 2581 tggatatttt atattgactt tcgtgtacat tgatcacatc gactgttcta ttggcaaatg 2641 aaccacgggc attgactatt tttcaggtta ctactatata ttattgttgg aataaaaatc 2701 aactatcatc tactaactag tatttacgtt actagtatat tatcatatac ggtgttagaa 2761 gatgacgcaa atgatgagaa atagtcatct aaattagtgg aagctgaaac acaaggattg 2821 ataatgtaat aggatcaatg aatattaaca tataaaatga tgataataat atttatagaa 2881 ttgtgtagaa ttgcagattc ccttttatgg tgttggaata aaaatcaact atcatctact 2941 aactagtatt tacgttacct agtatattat catatacggt gttagaagat gacgcaaatg 3001 atgagaaata gtcatctaaa ttagtggaag ctgaaacgca aggattgata atgtaatagg 3061 atcaatgaat attaacatat aaaatgatga taataatatt tatagaattg tgtagaattg 3121 cagattccct tttatggatt cctaaatcct cgaggagaac ttctagtata tctacatacc 3181 taatattatt gccttataaa aaatggaatc ccaacaatta catcaaaatc cacgttcttt 3241 tcatatggat tcctaaatcc tcgaggagaa tttataatat agtctgtata cataatatta 3301 tagcttctaa cgacaatgga attctaacaa ttatcaaatt gccgccagtt cctcaaaaag 3361 atccatgtat aatcttcatt attacagccc tcttgacctc taatcatgaa tgttctcaaa 3421 taaatctgtt ggaataaaaa tcaactatca tctactaact agtatttacg ttactagtat 3481 attatcatat acggtgttag aagatgacgc aaatgatgag aaatagtcat ctaaattagt 3541 ggaagctgaa acgcaaggat tgataatgta ataggatcaa tgaatattaa catataaaat 3601 gatgataata atatttatag aattgtgtag aattgcagat tcccttttat ggattcctaa 3661 atcctcgagg agaacttcta gtatatctac atacctaata ttattgcctt ataaaaaatg 3721 gaatcccaac aattacatca aaatccacgt tctcttcatg gtagcgccta tgcttcggtt 3781 acttctaagg aagtcccatc aaatcaagat ccgttagccg tttcagcttc caatttaccg 3841 gaatttgata gagattccac taaggttaat tctcaacaag agacaacacc tgggacatca 3901 gctgttccag agaaccatca tcatgtctct cctcaacctg cttcagtacc acctccacag 3961 aatggacagt accaacagca cggcatgatg accccaaaca aagctatggc ctctaactgg 4021 gcacattacc aacaaccgtc tatgatgacg tgttcacatt atcaaacgtc acctgcgtat 4081 tatcaaccgg acccacacta tccgttgcca cagtatatcc caccactgag tacttcctca 4141 cctgatccaa tcgattcaca gaatcaacac tctgaagtac ctcaagctga gacaaaggtg 4201 agaaataacg tcttaccacc acacacttta acatcagatg aaaacttttc tacatgggtt 4261 aaattttaca tcagattttt gaagaactct aatctcggtg acatcattcc aaatgaccag 4321 ggtgaaatca aaagacaaat gacttatgaa gaacatgcgt atatatacaa taccttccaa 4381 gcatttgccc catttcattt attgccaaca tgggtaaaac aaattttaga aattaattat 4441 gctgacatcc ttacagtcct ttgtaaaagt gtgtccaaaa tgcaaactaa caatcaagaa 4501 ttaaaggatt ggatagctct tgccaacctt gagtacgacg gaagtacatc tgctgataca 4561 tttgaaatta cagtcagtac gatcattcag aggctaaaag aaaacaatat caatgttagc 4621 gacagattgg cctgtcaact aatacttaaa ggtctatccg gtgacttcaa atacctacgt 4681 aatcaatatc gtaccaaaac gaacatgaaa ctttcccaat tattcgctga aattcagtta 4741 atatatgacg aaaataaaat catgaatcta aataaaccgt cccaatacaa acaacacagc 4801 gaatacaaaa atgtttctcg cacatctcca aacacgacta acacaaaggt tacaactcgt 4861 aattatcaga gaacaaatag ttcaaaacca agagcagcaa aagctcacaa tattgctaca 4921 tctagtaaat tctcaagggt gaacaatgat cacattaatg aatcaaccgt ttcatcacaa 4981 tacttaagcg atgacaacga acttagtctt aggccagcaa cagaaagaat ctaagccaac 5041 acacacaata gactcgaatg acgaactacc tgatcacctt cttattgatt caggacgttc 5101 gcaaacgctt gtcagatcag cccattattt acaccatgca acacccaatt ctgaaataaa 5161 catagtcgat gctcaaaaac aagacattcc tataaatgcc attggtaatc ttcacttcaa 5221 ctttcagaac ggcaccaaaa catcaataaa agcactacac acaccaaaca tagcctatga 5281 tctattaagt ttgagtgagc tggctaatca aaatattaca gcctgcttta ccagaaacac 5341 tttagaaaga tcggatggta cagtcctagc tcccatagtc aaacatggag acttttactg 5401 gttatctaaa aaatacctaa ttccttcgca catttcaaag ctaacaataa acaacgtcaa 5461 caaaagcaaa agcgtaaata aatatccata tccgttaata catcgaatgc ttggacatgc 5521 taacttccga agtattcaga agtctcttaa gaagaatgca gttacatatt tgaaggaatc 5581 ggatattgaa tggtctaacg catgcacata tcaatgtcct gactgtctaa tcggcaaaag 5641 cacgaaacat agacatgtca aaggatcacg actaaagtac caagaatcat atgagccttt 5701 tcagtacttg cataccgata tatttggtcc tgtacatcac ttaccgaaaa gtgcaccttc 5761 ttactttata tcgtttacag atgagaaaac cagattccaa tgggtgtacc cattacacga 5821 ccgtcgtgaa gaatctatcc tcaatgtttt tacatcgata ttagcattta ttaagaacca 5881 attcaatgct cgcgttctag ttatccagat ggatcgtggc tccgagtaca ctaacaaaac 5941 tcttcataag ttctttacga acagaggtat tactgcatgc tatacaacca cggcagattc 6001 tagagcacac ggtgtcgctg aacgattaaa tcgtacttta ttaaacgatt gtcgcacact 6061 gcttcattgc agtggtctac caaatcatct atggttctca gcagtcgaat tttctactat 6121 aatcagaaat tcattagtct caccaaaaaa cgataaatcc gccgcaagac aacatgcagg 6181 tttagctgga ctggacatta ctactatact acctttcggt caaccggtta tagttaacaa 6241 ccataatccc gactcgaaaa tacatcctcg tggcattcca ggttacgcct tacatccgtc 6301 acgaaactct tatggctata ttatctatct tccatcatta aaaaagacag tagatactac 6361 caattacgtt atattacaag acaagcaatc caaattggac caattcaatt atgatacact 6421 cacctttgat gatgatctca atcgtttaac agcccataac caatctttta ttgaacaaaa 6481 tgaaacggag cagtcatatg atcaaaatac agaatctgat catgactatc aatcggagat 6541 tgaaataaac tctgatcctc tagtgaacga cttctcgtcc caatcaataa accctttaca 6601 attagacaag gaaccagtcc aaaaagtacg tgcaccaaaa gaagttgatg ccgacatatc 6661 tgaatacaat attcttccat ctcctgtacg atctcgtaca ccccatatca ttaataaaga 6721 gagtaccgaa atgggtggta ccgttgaatc agatactact tcacctagac actcgtctac 6781 cttcactgca cgaaaccaaa agcgacctgg tagtcccaat gatatgattg atttgacctc 6841 acaggataga gttaattatg gacttgaaaa catcaaaact acacgtttgg gtggtacgga 6901 ggaaccatat attcaacgaa atagtgatac aaatatcaaa tacaggacta caaatagtac 6961 gccctcaata gatgaccgtt cgtccaacag tgaatccact actcccatca tctccataga 7021 aacaaaggct gtatgtgata atacaccctc cattgatacg gatccgccag aatatcgatc 7081 ttctgaccat gcgactccta atataatgcc tgacaaatcc tcaaaaaatg ttacggctga 7141 ttctattctt gacgacctcc cacttcctga cttaacccat caatctccta cggacacttc 7201 tgatgtttca aaagatattc cacacataca ctctcgtcag actaattcca gtttgggtgg 7261 tatggatgat tctaatgttc tgactactac caaaagtaag aaaagatcat tagaagataa 7321 tgaaactgaa attgaggtat cccgagacac atggaataat aagaatatga gaagtctgga 7381 accaccaaga tcgaagaaac gcataaattt aattgcagca ataaaaggag tgaaatcgat 7441 caaaccagtt cgaacgacct taagatatga tgaagcaatt acttataata aagacaacaa 7501 agaaaaagac agatatgttg aagcttatca taaagaaatt agccaactat tgaaaatgaa 7561 cacttgggat acaaacaaat attatgatag aaatgacata gatcctaaaa aagtaataaa 7621 ctcaatgttt atatttaaca agaaacgtga tggtacacac aaagctagat ttgttgcaag 7681 aggcgacatt caacaccccg atacatatga ttctgatatg caatccaata ccgtacatca 7741 ctatgcactg atgacgtcac tgtcaatcgc attagacaac gactattata tcacacagct 7801 ggacatatcc tctgcttact tatatgctga tatcaaagaa gaattataca taagacctcc 7861 accacattta ggtttgaatg ataaattact acgtttgaga aaatcactct atggtttgaa 7921 acaaagtggt gcaaactggt atgaaaccat taaatcatat ttaataaatt gttgcgacat 7981 gcaagaagtt cgcggatggt catgcgtatt taagaatagt caagtaacaa tttgcttatt 8041 cgttgatgat atgatattat tcagcaaaga cttaaatgca aataagaaaa tcataacaac 8101 actcaagaaa caatacgata caaagataat aaatctgggt gaaagtgata acgaaattca 8161 gtacgacata cttggattag agatcaaata tcaaagaagc aagtacatga aattaggtat 8221 ggaaaaatcc ttgacagaaa aattacccaa actaaacgta cctttgaacc caaaaggaaa 8281 gaaacttaga gctccaggtc aaccaggtca ttatatagac caggatgaac tagaaataga 8341 tgaagatgaa tacaaagaga aagtacatga aatgcaaaag ttgattggtc tagcttcata 8401 tgttggatat aaatttagat ttgacttact atactacatc aacacattgc tcaaccatat 8461 actattcccc tctaggcaag ttttagacat gacatatgag ttaatacaat tcatgtggga 8521 cactagagat aaacaattaa tatggcacaa aaacaaacct accaagccag ataataaact 8581 agtcgcaata agcgatgctt catatggtaa ccaaccatat tacaagtcac aaattggtaa 8641 cattttccta ctcaacggaa aagtgattgg aggaaagtcg acaaaggctt cgttaacatg 8701 cacttcaact acagaagcag aaatacacgc ggtcagtgaa gctattccgc tattgaataa 8761 cctcagtcac cttgtgcaag aacttaacaa gaaaccaatt attaaaggct tacttactga 8821 tagtagatca acgatcagta taattaagtc tacaaatgaa gagaaattta gaaacagatt 8881 ttttggcaca aaggcaatga gacttagaga tgaagtatca ggtaataatt tatacgtata 8941 ctacatcgag accaagaaga acattgctga tgtgatgaca aaacctcttc cgataaaaac 9001 atttaaacta ttaactaaca aatggattca ttagatctat tacattatgg gtggtatgtt 9061 ggaataaaaa tcaactatca tctactaact agtatttacg ttactagtat attatcatat 9121 acggtgttag aagatgacgc aaatgatgag aaatagtcat ctaaattagt ggaagctgaa 9181 acgcaaggat tgataatgta ataggatcaa tgaatattaa catataaaat gatgataata 9241 atatttatag aattgtgtag aattgcagat tcccttttat ggattcctaa atcctcgagg 9301 agaacttcta gtatatctac atacctaata ttattgcctt attaaaaatg gaatcccaac 9361 aattacatca aaatccacat tctcttcaaa atcaattgtc ctgtacttcc ttgttcatgt 9421 gtgttcaaaa acgttatatt tataggataa ttatactcta tttctcaaca agtaattggt 9481 tgtttggccg agcggtctaa ggcgcctgat tcaagaaata tcttgaccgc agttaactgt 9541 gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc attatttttt 9601 tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg 9661 aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac tgaaaaattg 9721 ggagaaaaag gaaaggtgag agccgcggaa ccggcttttc atatagaata gagaagcgtt 9781 catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa ggacctattg 9841 ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac cttttacatt 9901 tcagcaatat atatatatat atttcaagga tataccattc taatgtctgc ccctaagaag 9961 atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc cattaaggtt 10021 cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt 10081 ggtggtgctg ctatcgatgc tacaggtgtc ccacttccag atgaggcgct ggaagcctcc 10141 aagaaggttg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg taccggtagt 10201 gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta cgccaactta 10261 agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa gccacaattt 10321 gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg gaggtattta ctttggtaag 10381 agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac cgttccagaa 10441 gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc accattgcct 10501 atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag aaaaactgtg 10561 gaggaaacca tcaagaacga attccctaca ttgaaggttc aacatcaatt gattgattct 10621 gccgccatga tcctagttaa gaacccaacc cacctaaatg gtattataat caccagcaac 10681 atgtttggtg atatcatctc cgatgaagcc tccgttatcc caggttcctt gggtttgttg 10741 ccatctgcgt ccttggcctc tttgccagac aagaacaccg catttggttt gtacgaacca 10801 tgccacggtt ctgctccaga tttgccaaag aataaggtca accctatcgc cactatcttg 10861 tctgctgcaa tgatgttgaa attgtcattg aacttgcctg aagaaggtaa ggccattgaa 10921 gatgcagtta aaaaggtttt ggatgcaggt atcagaactg gtgatttagg tggttccaac 10981 agtaccacgg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa 11041 gattctcttt ttttatgata tttgtacata aactttataa atgaaattca taatagaaac 11101 gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat acgcaatcta 11161 catacattta tcaagaagga gaaaaaggag gatgtaaagg aatacaggta agcaaattga //