GenBank-Updates@genbank.bio.net (05/09/91)
LOCUS ECORRG01 129 bp ss-rRNA RNA 09-MAY-1991
DEFINITION E.coli 23S ribosomal RNA (approx 80%), fragment A.
ACCESSION K01107
KEYWORDS 23S ribosomal RNA; ribosomal RNA.
SEGMENT 1 of 26
SOURCE Escherichia coli rRNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 129)
AUTHORS Branlant,C., Krol,A., Machatt,M.A. and Ebel,J.-P.
TITLE Structural study of ribosomal 23 S RNA from Escherichia coli
JOURNAL FEBS Lett. 107, 177-181 (1979)
STANDARD full staff_review
COMMENT [FEBS Lett. 107, 177-181 (1979)] found difficulty in obtaining
overlapping fragments
encompassing the whole 23S rRNA molecule. There are about 60
sequence differences between given sequence and that derived from
the 23S rRNA gene sequence <ecorgnb>. Microheterogeneity between
different rRNA operons may explain some of the discrepancies.
Another significant difference is that the alignment of large
fragments by [FEBS Lett. 107, 177-181 (1979)] is not in agreement
with the gene sequence.
Finally, some overlapping fragments were located distantly from
each other in the structure, so that some sections of the molecule
appear in two different locations in given sequence. Sequence
heterogeneities found by [FEBS Lett. 107, 177-181 (1979)] are shown
as variations in the Sites
Table.
FEATURES Location/Qualifiers
rRNA 1..>129
/note="23S rRNA"
BASE COUNT 36 a 25 c 42 g 26 t
ORIGIN 5' end of mature rRNA.
1 ggttaagcga ctaagcgtac acggtggatg ccctggcagt cagaggcgat gaaggacgtg
61 ctaatctgcg ataagcgtcg gtaaggtgat atgaaccgtt ataaccggcg atttccgaat
121 gggaaacag
//