GenBank-Updates@genbank.bio.net (05/09/91)
LOCUS ECORRG22 102 bp ss-rRNA RNA 09-MAY-1991 DEFINITION E.coli 23S ribosomal RNA, fragment S/T. ACCESSION K01128 KEYWORDS 23S ribosomal RNA; L1 protein; binding site; ribosomal RNA. SEGMENT 22 of 26 SOURCE Escherichia coli (strain MRE 600 [Eur. J. Biochem. 70, 471-482 (1976)]) rRNA. ORGANISM Escherichia coli Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 63 to 102) AUTHORS Branlant,C., Korobko,V. and Ebel,J.-P. TITLE The binding site of protein L1 on 23-S ribosomal RNA from Escherichia coli: 3. Nucleotide sequence JOURNAL Eur. J. Biochem. 70, 471-482 (1976) STANDARD full staff_review REFERENCE 2 (bases 1 to 102) AUTHORS Branlant,C., Krol,A., Machatt,M.A. and Ebel,J.-P. TITLE Structural study of ribosomal 23 S RNA from Escherichia coli JOURNAL FEBS Lett. 107, 177-181 (1979) STANDARD full staff_review COMMENT [Eur. J. Biochem. 70, 471-482 (1976)] see comment. See segment 1. [Eur. J. Biochem. 70, 471-482 (1976)] shows the almost complete sequence of a region of the 23S rRNA containing the L1 protein binding site, and suggests possible secondary structure models. The sequence is 175 bp in length. [Eur. J. Biochem. 70, 471-482 (1976)] proposes that it is located between the 550th and 1000th nucleotide at the 3' end of the 23S RNA. Comparison between [Eur. J. Biochem. 70, 471-482 (1976)] and [FEBS Lett. 107, 177-181 (1979)] sequence data, however, shows very little homology, the most homologous region being between bases 63 and 102 of this segment. Given sequence is that of [FEBS Lett. 107, 177-181 (1979)] . FEATURES Location/Qualifiers rRNA <1..>102 /note="23S rRNA" modified_base 44..44 /note="m7g = 7-methylguanosine" conflict 73..73 /location=Eur. J. Biochem. 70, 471-482 (1976) | 11..11 BASE COUNT 27 a 22 c 31 g 22 t ORIGIN 1 tgtgcagatg cagtgtaccc gcggcaagac ggaaagaccc cgtgaacctt tactatagct 61 tgacactgaa cactgagcct tgatgtgtag gataggtggg ag //