GenBank-Updates@genbank.bio.net (05/09/91)
LOCUS HRSIFNAA 3243 bp ds-DNA MAM 09-MAY-1991
DEFINITION Horse interferon-alpha-1 gene, complete cds.
ACCESSION M14540
KEYWORDS alpha-interferon; interferon.
SOURCE Horse liver DNA, clone lambda-Eq-alpha-1, subclone pAH50.
ORGANISM Equus caballus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Perissodactyla; Equidae.
REFERENCE 1 (bases 1 to 3241)
AUTHORS Himmler,A., Hauptmann,R., Adolf,G.R. and Swetly,P.
TITLE Molecular cloning and expression in Escherichia coli of equine type
I interferons
JOURNAL DNA 5, 345-356 (1986)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 3243)
AUTHORS Hauptmann,R.
JOURNAL Unpublished (1987) Ernst Boehringer Inst, Vienna, Austria, A-1121.
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [DNA 5, 345-356
(1986)],[Unpublished (1987) Ernst Boehringer Inst, Vienna, Austria,
A-1121.] kindly
provided by R.Hauptmann, 23-FEB-1987.
Polyadenylation signals are located at positions 1338-1343,
1417-1422, and 1946-1951 and a Goldberg-Hogness box is found at
333-339.
FEATURES Location/Qualifiers
CDS 425..979
/note="interferon-alpha-1 precursor"
/codon_start=425
sig_peptide 425..493
/note="interferon-alpha-1 signal peptide"
/codon_start=425
mat_peptide 494..976
/note="interferon-alpha-1"
/codon_start=494
BASE COUNT 1066 a 600 c 675 g 902 t
ORIGIN 1 bp upstream of HindIII site.
1 aagcttaaaa ttttagatca ttcttaatct tgcagtgaag aaaaagagta aagtttacac
61 tttctttttc taagtataaa gtagcgatag gcagggtgca cataaacaga tataccgtat
121 atctgtgtta ctaagttttc ttgagggctt caattaggaa aacatctaaa aagagtctgg
181 cagcaggagg caataacaac aacgacaaaa tatggtcaag aaacactgcc ctacaaccac
241 atcaacatct acttcatata gaaagcacat aaagaaacta aaaacagaag tagaaagtaa
301 gggaaacatg cagaaaatgg aaactagttc cctatttaag acacatgcac aaaggaaggt
361 cttcagagaa cccagagacc aaggctcaca gggtcaccca ccagcagcat ctgcaagatc
421 cccaatggct ctgcctgttt ccttactgat ggccctggtg gtgctcagct gccactccat
481 ctgctctctg ggatgtgacc tgcctcacac ccatagcctg ggcaacacaa gggtcttgat
541 gctcctgggg caaatgagga gaatctcccc cttctcctgc ctgaaggaca gaaatgactt
601 tggattcccc caggaggtgt ttgacggcaa ccagttccgg aagcctcaag ccatctctgc
661 ggtccatgag acgatccaac agatcttcca cctcttcagc acagacggct cgtctgccgc
721 ctgggacgag agcctcctag acaaactcta cactggactc tatcagcagc tgactgagct
781 ggaagcctgt ctgagccagg aggtgggggt ggaagagacg cccctgatga acgaggactc
841 cctgctggct gtgaggagat acttccaaag aatcgctctc tatctgcaag agaagaaata
901 cagcccttgt gcctgggaga tcgtcagagc agaaatcatg agatccttct cttcatccac
961 aaacttgccg cagagttaag gaggaagaaa tgacacctgg ttcaacatgg aaatgcttct
1021 cattgactga taatatcaca cttccacttg ctctgccatc tcaaggactc tcatgtctgc
1081 tgtaatcatg acctgaattg aatcaatttt tcaaatgttt tcagtagtat taatgaatgt
1141 tgggtctaac cctgtggaca ttagtctgat acagacgacc atgttgatct atttatttat
1201 ttatttacat atttatttaa ttatttatga gatttaaatt atttttgttg ctataacatt
1261 atgtgcacct ttacactgta gtttaatata acaaaatgta tgcttcatat ttagcctatt
1321 tattattttc tgtgttcatt aaatctttac tgtagaaaat atcttctatt tgtttattct
1381 ttaaaagaga aacaccacac ctgagtgtgc aagctgatta aagaatggat ggcactattc
1441 atttaccatc attgtcatat tcaagttaga agtaaaaata gacttcctct aagcctggtt
1501 gtatgttgac ctcaggatat gaggtgaaca caacaaatac agttcctgct ttcttgaatg
1561 tttgtttttt ctggggaagg taacctaaaa acactaacca attgattggt aattgaatat
1621 taattatttt taaattaaca ctaattaata ttagaaatta ttatcagtta ggtttaatgc
1681 taaatgggaa aggaaaaaaa tggagtgcaa atgtccgctg gatattcgag tttgcaattt
1741 acaagcaatt ccatgaactg taggtggaat tgaagtagga atggaagtgg tcacatggga
1801 agagacgaca gaatgagaaa aggacttaaa attgattcct aggaccttca accttaaaag
1861 ggaaggacaa gccataaagg aaacaggtgg aatggccata tgttaacagg acgagagaga
1921 atggtgagat gaccatgttt aaggaattaa actgcttaga agactcaatg cattcatgaa
1981 aaatgtggat ggaaactgcc caccacgctg ggaaaggaga gcgcattggc gacctcaggg
2041 agagctagtg tggtggaatt caccaccaga aagcacactg gagtaggagg aaaggtgaat
2101 aagagaagtt aatgacaata tatagaaaat ggttttgaga cctttgcctt ctgaagagga
2161 ggagaggaat aaggcaggac ctagaataga gtgtggatta ttttttctct gcatcgtttt
2221 cccttttttg aaatacactt gcttctggat agatttcacg gctttgatgt attgacaaat
2281 catattaata tgtttaatgt tttatttact ttaaatcatg aaatatttaa ttacttacaa
2341 tgataattta tcattattta gattagtact atgttgaatg ccagtgggct gctctgtgga
2401 ttgaaaccat ccactacacg gggaaacaaa tggaacagac cttagtgaga gctagtttgg
2461 tggaactaat gagcagaaac cacattggag tagcggaaag gtgataagag aagaactccc
2521 attgtcattt ttctcacgtg tgggctcact ttatttctgt catcatattc agacctacat
2581 ttattctcct tttgcctcag tgcatccaaa tgcaaggata gctttctaag aaagctgaaa
2641 ggggaaggaa acaagtgatg tattgaaaat gatggaaaat aatagcacag gtctcttaag
2701 tttccatttt tacttcccta ttttggtact aacctcaaga tgaaatagga ggtaattcag
2761 atgcagcaag acaactcttg ccaaggaaaa tactccatta aaatgaacat acaaaatgat
2821 aaaaaccctg aaactagtaa agcaaagatg gcaagtttcc tgtaacacca caaggcagtc
2881 tcactgtcct acattcttca ctgtgagatg gagaaagatg aacctagaag ctctgtggaa
2941 atgtactgac aaataatcta gggatgatta attaaatctt gagtaattat tatgaactat
3001 gtgttaacga tagcatagtg aacaagatat agacctttct cctgaggagg tttcttgcca
3061 aacaggtcta atagatccat tttcaagaag acaaatccag aagggtaagg cagcctggct
3121 gacatgcaaa acaaagaggg caaaagagag ctcaggatca gaggagaaat tttgttctta
3181 gcagaagaac actatttaca taagtctaag tttaacaaaa aaactttcct catttgaaag
3241 ctt
//