GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS CHKMYLCZ2F 689 bp ss-mRNA VRT 10-MAY-1991
DEFINITION Chicken myosin light chain 2 (LC2f) mRNA, complete cds.
ACCESSION M11030
KEYWORDS myosin.
SOURCE Chicken (White Leghorn) pectoralis major muscle, cDNA to mRNA,
clonelambda-gt11/L10.
ORGANISM Gallus gallus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Aves;
Neornithes; Neognathae; Galliformes; Phasianidae.
REFERENCE 1 (bases 1 to 689)
AUTHORS Reinach,F.C. and Fischman,D.A.
TITLE Recombinant DNA approach for defining the primary structure of
monoclonal antibody epitopes
JOURNAL J. Mol. Biol. 181, 411-422 (1985)
STANDARD full staff_review
COMMENT Draft entry and clean copy sequence for [J. Mol. Biol. 181, 411-422
(1985)] kindly provided by
F.Reinach, 14-AUG-1985.
Using a monoclonal antibody (MF5) that recognizes a reversible
divalent metal-induced conformational change in LC2f makes it
possible to establish the antibody binding site within the primary
structure of the protein and thereby locate a region that undergoes
conformational change upon metal-binding. Three DNA regions were
located using this method, of which two produce protein that is
reactive with the antibody but is not the immunogen. The sequence
presented here is that coding for the immunogen. The binding sites
for the monoclonal antibodies are located in the first 38 amino
acids of chicken myosin light chain 2 and AAs 5 through 13 are
unnecessary for antibody binding.
Two poly-C regions are found at 552-568 and 646-666. A partially
conserved repeat sequence (550-664) is located between these two
poly-C regions. A polyadenylation signal is located at positions
677-682.
FEATURES Location/Qualifiers
mRNA <1..689
/note="LC2f mRNA"
CDS 25..531
/note="myosin light chain type 2 (LC2f)"
/codon_start=25
BASE COUNT 154 a 233 c 190 g 112 t
ORIGIN 98 bp upstream of MboI site.
1 gcgaccccac tgacgagcag cgctatggcc cccaagaagg cgaagcgccg cgcagccgag
61 ggctcctcca atgtcttctc tatgttcgac cagacccaga tccaggagtt caaagaggcg
121 ttcacggtga tcgatcagaa ccgcgatggc atcattgata aggacgacct gcgggagacc
181 ttcgccgcca tggggcgcct gaacctgaag aacgaggagt tggacgccat gatcaaggag
241 gcgagcggcc ccattaactt caccgtcttc ctcaccatgt ttggggagaa gctgaagggc
301 gctgaccccg aagacgtcat catgggggcc ctcaaagtgc tcgaccccga tgggaaggga
361 tccatcaaga agagcttcct ggaggagctg ctgaccacac agtgcgaccg cttcaccccc
421 gaggagatca agaacatgtg ggccgccttc ccccccgacg tggcgggcaa cgtggactat
481 aagaacatct gctacgtcat cacccacggg gaggacaaag agggggaatg accccaaaac
541 cgggaccccc cccccccccc ccatatggga ccccccgtgg gacccctatg ggacccctta
601 tgggatcctt ctgggacccc ttatgggatc cttttgggac cccccccccc cccccccccc
661 ggctatccca attaaagcga tgcttttcc
//