GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS PBR322P1 84 bp ds-DNA SYN 10-MAY-1991 DEFINITION Plasmid pBR322, pre-penicillinase signal peptide, partial cds with PstI site. ACCESSION M10537 KEYWORDS . SOURCE Plasmid pBR322 from E.coli DNA. ORGANISM Cloning vector Artificial sequences; Cloning vehicles. REFERENCE 1 (bases 1 to 84) AUTHORS Talmadge,K. and Gilbert,W. TITLE Construction of plasmid vectors with unique PstI cloning sites in a signal sequence coding region JOURNAL Gene 12, 235-241 (1980) STANDARD simple staff_review FEATURES Location/Qualifiers CDS 1..>84 /note="penicillinase" /codon_start=1 sig_peptide 1..69 /note="pre-penicillinase signal peptide" /codon_start=1 mat_peptide 70..>84 /note="penicillinase, mature peptide" /codon_start=70 BASE COUNT 13 a 24 c 14 g 33 t ORIGIN 1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 61 gtttttgctc acccagaaac gctg //