GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS STYCYSJIH 3876 bp ds-DNA BCT 10-MAY-1991
DEFINITION S.typhimurium cysJ, cysI and cysH genes, complete cds.
ACCESSION J05009
KEYWORDS 3'phosphoadenosine 5-phosphosulfate sulfotransferase; flavoprotein;
hemoprotein; sulfite reductase; sulfotransferase.
SOURCE E.coli (strain B; ATCC 11303) DNA, clone pJYW2.
ORGANISM Salmonella typhimurium
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 3876)
AUTHORS Ostrowski,J., Wu,J.-Y., Rueger,D.C., Miller,B.E., Siegel,L.M. and
Kredich,N.M.
TITLE Characterization of the cysJIH regions of Salmonella typhimurium
and Escherichia coli B: DNA sequences of cysI and cysH and a model
for the siroheme-Fe4S4 active center of sulfite reductase
hemoprotein based on amino acid homology with spinach nitrit
JOURNAL J. Biol. Chem. 264, 15726-15737 (1989)
STANDARD full staff_review
COMMENT Draft entry computer-readable copy of sequence [J. Biol. Chem. 264,
15726-15737 (1989)] kindly submitted
by N.M.Kredich 16-AUG-1989.
FEATURES Location/Qualifiers
CDS <1..21
/note="CysJ flavoprotein component of sulfite reductase
(EC 1.8.1.2) /nomgen='cysJ'"
/codon_start=1
CDS 21..1733
/note="CysI hemoprotein component of sulfite reductase (EC
1.8.1.2) /nomgen='cysI'"
/codon_start=21
repeat_region 1750..1785
/note="repetitive extragenic palindromic sequence"
CDS 1809..2543
/note="CysH 3'phosphoadenosine 5'-phosphosulfate
sulfotransferase /nomgen='cysH'"
/codon_start=1809
BASE COUNT 980 a 936 c 1024 g 936 t
ORIGIN 84 bp upstream of HindIII site.
1 tatcagcgag atgtctacta atgagcgaaa aacatccagg cccactggtg gtcgaaggta
61 aactgtccga tgccgagcgc atgaagctgg aaagcaacta tctgcgcggc accattgcgg
121 aagatctgaa cgacggtctc accggcggtt ttaaaggcga taacttcctg ctgatacgct
181 ttcatggcat gtatcagcag gatgaccgcg atattcgcgc cgagcgtgcg gcgcagaagc
241 tggagccgcg ccacgcgatg ctgctgcgct gccgcctgcc gggcggggtg attactacga
301 cgcaatggca ggcgatcgat aaattcgcgg cggataacac gatttacggc agtatccgtc
361 tgaccaaccg ccagaccttc cagttccacg gcattctgaa gaagaacgtt aagccggtgc
421 accagatgct gcactcggtg gggctggatg cgctggcaac cgccaacgac atgaaccgta
481 acgtgctgtg tacctctaac ccgtacgagt ctcagctgca tgctgaagcc tacgaatggg
541 cgaagaagat ctccgaacat ctgctgccgc gcacccgtgc gtatgcggag atctggctcg
601 atcaggagaa ggtcgcgacc accgatgaag aaccgatcct cgggcagacc tatctgccgc
661 gtaaatttaa aaccacggtg gtgatcccgc cgcaaaatga tatcgatctg cacgccaacg
721 acatgaactt tgtggcgatt gccgagaacg gcaagctggt cggctttaac ctgctggtgg
781 gcggcggtct gtctattgag catggtaata agaaaaccta cgctcgtacg gccagcgagt
841 ttggctatct gccgctggag cacaccctgg cggtggccga agcggtagtg acgacgcagc
901 gcgactgggg taaccgtacc gacaggaaaa atgccaaaac caaatacacc cttgagcgcg
961 tgggactgga gacatttaaa gcggaagtcg agcgtcgcgc ggggatcaaa tttgagccga
1021 tccgcccgta tgagtttacc gggcgtggcg acaggattgg ctgggtgaaa ggcattgatg
1081 ataaatggca cctgacgctg tttattgaga acggccgtat cctggattat ccggggcgtc
1141 cgctgaaaac cggcctgctg gagatcgcga agattcatca gggcgagttc cgtatcaccg
1201 ctaaccagaa cctgattatt gccagcgtgc cggaaagcca gaaagcgaag atcgagacgc
1261 tggcgcgcga ccacgggttg atgaatgcgg tgaaaccgca gcgtgaaaac tcaatggcct
1321 gcgtgtcgtt cccaacctgc ccgctggcaa tggcggaagc cgagcgtttc ctgccgtcgt
1381 tcacggacaa agtggaagcg attctggaaa aacacggcat tcctgatgag catatcgtga
1441 tgcgcgtgac cggctgcccg aacggctgtg gccgcgcgat gctggcggaa attggcttag
1501 tagggaaagc gcctggtcgt tataacctgc atcttggtgg caaccgcatc gggagtcgta
1561 tcccacggat gtataaagaa aatatcgccg agccggatat tctggcctcg ctggatgaac
1621 tgattgggcg ctgggcgaaa gagcgtgaag cgggtgaagg cttcggcgac tttacggtgc
1681 gtgcgggcat cattcgcccg gtgctcgatc ccgcgcgtga tttctgggaa taacctgaat
1741 tgccggatgg cgtctgtgta ggcctgataa gcacagcgcc atcaggcaag gcaaacagtg
1801 aggaactcat gtcccaactc gatctcaacg cattgaatga attgccaaaa gtcgatcgcg
1861 taatggctct ggcggaaacc aacgcccaac tggaaaaact gagcgccgaa gaacgcgtgg
1921 cctgggcgct ggaaaatctg cccggcgaat acgtgctctc ctcaagcttt ggcatccagg
1981 cggcggtgag cctgcacctg gtgaaccaga ttcgcccgga tatcccggtg atcctcaccg
2041 ataccggcta tttgttcccg gaaacctacc agtttattga tgagctaacg gacaaactca
2101 aactcaacct gaaagtttac cgcgcgggcg aaagtccggc ctggcaggaa gcgcgctacg
2161 gcaagctgtg ggagcagggc gttgagggca ttgagaaata caatgagatc aacaaagtcg
2221 agccgatgaa ccgggcgcta aaagagctga aagcgcagac gtggtttgcg ggtctgcgtc
2281 gcgagcagtc cggcagccgc gcgcatttac ccgttctcgc tatccagcgc ggcgtgttta
2341 aagtgctacc gattatcgac tgggataatc gcaccgttta ccagtacctg caaaagcatg
2401 gcctgaaata ccatccgctg tgggatcagg gctatctgtc ggtgggcgat acgcacacca
2461 cccgcaaatg ggagccgggt atggcggaag aagagacacg cttcttcggg ctgaagcgcg
2521 agtgcgggct gcatgaaggg taattgtcct tacgcggtat gattatactt gctgctatga
2581 aaaatccggc aggcagccgg attttaaatt ggttatatta ctgactatct ttatgtcagt
2641 aatatattac gactgcaccc atctttacca atgtgcaaag aacgttcatg ggttagatat
2701 agtttttcaa gaattacatc aataaacatt ttttttgcat gatagtcgtt ataatcctga
2761 ttaagttttt ttaaaaaacc agaatttgtt ggtttaaact ctgaaaacgg tgaatagcta
2821 tgtagtaaat aggagatgca cggatgcatc atgctaaaag cgacttcacc ttgtacctcc
2881 tgataaccta ttttgcaaaa ctgagtcgta ggctcaaagt taccggcatt aatacaggcc
2941 agcactgatt tttcatctgc agtaagtgag tcctgccatt cgacaagcgg gtggtctgga
3001 gcctctgcgg gacgcatcat ctcataaacc gccgttttga tttgtgcatt tcccgtcact
3061 tcttttcgta gagttgaaat cgactcctga agtaattccg ggtgccaggt catcttcgaa
3121 ccaatcacct cacatatttg taaaaataat tgtctctcct ctctactcat agattctatc
3181 actgcatcat ctgatatatt aaggatgtcc ttaatattgc tttggctgat caccgtgtcg
3241 ccgaccataa gtaaaaattg tgtctgagaa gcgtccatct tgatagtaaa cagatcttga
3301 tgagccgggc aggccagatg ttgtagccgt ttaaaggcgt aaattttatt tatatcctct
3361 acgttaactt ccatctctcc aggttcacca cggccctgcc cgtgaatgat ggagtgtaac
3421 acttccagcg catggtcctt tttttccgct ctaaaatgat cctggacttt atcccaaccc
3481 cccatatgga ttgctttttc tttatctgcg cttaacagat gagcaagccg actttcatta
3541 agcgtataat tttgatgatt accgaagctt aaagtgactg gcataatatt ttccttcaaa
3601 tttatattat tcaatatccg gagtaaaata tatggttcaa ttctctcttt tttattaaac
3661 agataaagtt tactacagga gagaatttga acgacattat taatacagca catttatggt
3721 tggtaatcct ttgtggtgac tataattcag acaaaaggta gatgattcgc cgcaaatggt
3781 cgtttttttt aaattttggt ttatgcagaa ggcattggag gagcctcttt gagcgcttct
3841 tcctgttgaa aaggcggaaa atttataata attatt
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