GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS ECOPHOAAZ 1772 bp ds-DNA BCT 10-MAY-1991
DEFINITION E.coli JM83 alkaline phosphatase gene, complete cds.
ACCESSION X04586
KEYWORDS alkaline phosphatase; phosphatase.
SOURCE Escherichia coli.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1772)
AUTHORS Shuttleworth,H., Taylor,J. and Minton,N.
TITLE Sequence of the gene for alkaline phosphase from Escherichia coli
JM83
JOURNAL Nucleic Acids Res. 14, 8689-8689 (1986)
STANDARD simple automatic
COMMENT
[Nucleic Acids Res. 14, 8689-8689 (1986)] enum. 1 to 1772.
*source: strain=JM83;
Data kindly reviewed (15-MAY-1987) by H. Shuttleworth
EMBL features not translated to GenBank features:
key from to description
PRM 208 213 put. -35 region
PRM 231 236 put. -10 region
RBS 271 274 put. rRNA binding site
TERM 1699 1736 stem-loop structure
FEATURES Location/Qualifiers
CDS 283..1698
/note="alkaline phosphatase /nomgen='phoA'"
/codon_start=283
BASE COUNT 472 a 439 c 475 g 386 t
ORIGIN
1 aagctttgga gattatcgtc actgcaatgc ttcgcaatat ggcgcaaaat gaccaacagc
61 ggttgattga tcaggtagag ggggcgctgt acgaggtaaa gcccgatgcc agcattcctg
121 acgacgatac ggagctgctg cgcgattacg taaagaagtt attgaagcat cctcgtcagt
181 aaaaagttaa tcttttcaac agctgtcata aagttgtcac ggccgagact tatagtcgct
241 ttgtttttat tttttaatgt atttgtacat ggagaaaata aagtgaaaca aagcactatt
301 gcactggcac tcttaccgtt actgtttacc cctgtgacaa aagcccggac accagaaatg
361 cctgttctgg aaaaccgggc tgctcagggc gatattactg cacccggcgg tgctcgccgt
421 ttaacgggtg atcagactgc cgctctgcgt gattctctta gcgataaacc tgcaaaaaat
481 attattttgc tgattggcga tgggatgggg gactcggaaa ttactgccgc acgtaattat
541 gccgaaggtg cgggcggctt ttttaaaggt atagatgcct taccgcttac cgggcaatac
601 actcactatg cgctgaataa aaaaaccggc aaaccggact acgtcaccga ctcggctgca
661 tcagcaaccg cctggtcaac cggtgtcaaa acctataacg gcgcgctggg cgtcgatatt
721 cacgaaaaag atcacccaac gattctggaa atggcaaaag ccgcaggtct ggcgaccggt
781 aacgtttcta ccgcagagtt gcaggatgcc acgcccgctg cgctggtggc acatgtgacc
841 tcgcgcaaat gctacggtcc gagcgcgacc agtgaaaaat gtccgggtaa cgctctggaa
901 aaaggcggaa aaggatcgat taccgaacag ctgcttaacg ctcgtgccga cgttacgctt
961 ggcggcggcg caaaaacctt tgctgaaacg gcaaccgctg gtgaatggca gggaaaaacg
1021 ctgcgtgaac aggcacaggc gcgtggttat cagttggtga gcgatgctgc ctcactgaat
1081 tcggtgacgg aagcgaatca gcaaaaaccc ctgcttggcc tgtttgctga cggcaatatg
1141 ccagtgcgct ggctaggacc gaaagcaacg taccatggca atatcgataa gcccgcagtc
1201 acctgtacgc caaatccgca acgtaatgac agtgtaccaa ccctggcgca gatgaccgac
1261 aaagccattg aattgttgag taaaaatgag aaaggctttt tcctgcaagt tgaaggtgcg
1321 tcaatcgata aacaggatca tgctgcgaat ccttgtgggc aaattggcga gacggtcgat
1381 ctcgatgaag ccgtacaacg ggcgctggaa ttcgctaaaa aggagggtaa cacgctggtc
1441 atagtcaccg ctgatcacgc ccacgccagc cagattgttg cgccggatac caaagctccg
1501 ggcctcaccc aggcgctaaa taccaaagat ggcgcagtga tggtgatgag ttacgggaac
1561 tccgaagagg attcacaaga acataccggc agtcagttgc gtattgcggc gtatggcccg
1621 catgccgcca atgttgttgg actgaccgac cagaccgatc tcttctacac catgaaagcc
1681 gctctggggc tgaaataaaa ccgcgcccgg cagtgaattt tcgctgccgg gtggtttttt
1741 tgctgttagc aaccagactt aatggcagat ca
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