GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS ECOCYSJIH 3651 bp ds-DNA BCT 10-MAY-1991
DEFINITION E.coli cysJ, cysI and cysH genes, complete cds.
ACCESSION J05057
KEYWORDS 3' phosphoadenosine 5'-phosphosulfate sulfotransferase;
flavoprotein; hemoprotein; sulfite reductase; sulfotransferase.
SOURCE S.typhimurium (strain LT7; isolate hisG70) DNA, clone pGBK5.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 3651)
AUTHORS Ostrowski,J., Wu,J.-Y., Rueger,D.C., Miller,B.E., Siegel,L.M. and
Kredich,N.M.
TITLE Characterization of the cysJIH regions of Salmonella typhimurium
and Escherichia coli B: DNA sequences of cysI and cysH and a model
for the siroheme-Fe4S4 active center of sulfite reductase
hemoprotein based on amino acid homology with spinach nitrit
JOURNAL J. Biol. Chem. 264, 15726-15737 (1989)
STANDARD full staff_review
COMMENT Draft entry and computer-readable copy of sequence [J. Biol. Chem.
264, 15726-15737 (1989)] kindly
submitted by N.M.Kredich, 16-AUG-1989.
FEATURES Location/Qualifiers
CDS <1..21
/note="CysJ flavoprotein component of sulfite reductase
(EC 1.8.1.2)"
/codon_start=1
CDS 21..1733
/note="CysI hemoprotein component of sulfite reductase (EC
1.8.1.2)"
/codon_start=21
repeat_region 1758..1787
/note="repetitive extragenic palindromic sequence"
CDS 1807..2541
/note="CysH 3' phosphoadenosine 5'-phosphosulfate
sulfotransferase"
/codon_start=1807
BASE COUNT 917 a 911 c 1003 g 820 t
ORIGIN
1 tatcagcgag atgtctacta atgagcgaaa aacatccagg gcctttagtg gtcgaaggaa
61 aactgacaga cgccgagcgc atgaagcttg aaagcaacta cctgcgcggc accattgcgg
121 aagatttaaa cgacggtctg accggcggct ttaagggcga caacttcctg ctgatccgct
181 tccacggcat gtatcagcag gatgaccgcg acatccgcgc cgaacgtgct gaacagaagc
241 tggagccgcg ccacgcgatg ctgttgcgct gccgtctgcc gggtggggtg attaccacta
301 aacagtggca ggcgatagac aaatttgccg gtgaaaacac catctatggc agcattcgcc
361 tgaccaaccg ccagacgttt cagttccacg gcattctgaa aaagaacgtc aaaccggtgc
421 accagatgct gcactcggtt ggtcttgatg cgctggcgac cgctaacgac atgaaccgta
481 acgtactctg cacctcgaac ccttacgagt cgcagctaca cgcggaagcg tacgagtggg
541 cgaagaagat ttctgagcat ctgctgccgc gtacccgcgc gtatgcggag atctggctcg
601 accaggaaaa agtcgccact actgatgaag aaccgatcct cggccagacc tacctgccgc
661 gtaaattcaa aaccacggta gtgatcccgc cacagaacga tatcgatctg cacgccaacg
721 acatgaactt cgtggcgatc gccgaaaacg gcaagctggt gggctttaac ctgttggtgg
781 gcggtgggct ttccatcgaa cacggcaaca agaaaaccta cgcccgcacc gcgagtgagt
841 ttggctatct gccgctggag catacgctgg cggtggccga agccgtcgtg acaactcaac
901 gtgactgggg taaccgaacc gatcgtaaaa atgccaaaac caaatacacg ctggagcgcg
961 tgggggttga gacgtttaaa gcggaagtgg agcgtcgcgc ggggatcaaa tttgaaccga
1021 tccgtccata tgagttcacc ggacgaggcg atcgtattgg ctgggttaag ggcattgatg
1081 ataactggca cctgacgctg tttatcgaaa atggtcgcat ccttgattat ccggcgcgtc
1141 cgctgaaaac cggcctgctg gagatcgcga agatccacaa aggcgatttc cgcattacgg
1201 cgaaccagaa tctgatcatc gccggtgtac cggaaagcga gaaagcgaag atcgagaaga
1261 tcgccaaaga gagcgggtta atgaatgccg tcacgccgca gcgtgaaaac tcaatggcct
1321 gcgtgtcatt cccgacttgc ccgctggcga tggcggaagc ggagcgtttc ctgccgtctt
1381 ttatcgacaa catcgataat ttaatggcga aacatggtgt cagcgatgag catatcgtga
1441 tgcgtgtaac aggctgcccg aacggttgtg gtcgcgcgat gctggcggaa gtgggcctgg
1501 tgggtaaagc gccgggtcgc tacaacctgc atcttggcgg caaccgcatt gggacacgta
1561 tccctcggat gtataaagaa aacatcaccg agccggaaat cctggcgtcg cttgatgaac
1621 tgatagggcg ctgggcgaaa gagcgcgaag cgggtgaagg cttcggcgac tttacggtgc
1681 gtgcgggcat cattcgcccg gtgctcgatc cggcgcgcga tttgtgggat taaccatcag
1741 cccggtcttg taggcctgat aagacgcgtg agcgtcgcat caggcaaggc aaacagtgag
1801 gaatctatgt ccaaactcga tctaaacgcc ctgaacgaac tgccgaaggt agatcgcatt
1861 ctggcgctgg cggaaactaa cgcccaactg gaaaaactgg acgctgaagg ccgcgtagcc
1921 tgggcgctgg ataatctgcc cggtgaatat gtgctttctt ccagcttcgg cattcaggcg
1981 gcggtgagcc tgcatctggt gaatcagatt cggccggata ttccagtgat cctcaccgat
2041 accggttatc tgtttccgga aacctaccgc tttattgacg agttaacgga caaactcaag
2101 ctcaacctga aagtgtaccg tgctaccgaa agcgccgcct ggcaggaagc acgctacggc
2161 aaactgtggg aacagggcgt tgaaggcatt gaaaagtaca atgacatcaa caaagtcgaa
2221 ccgatgaacc gggcgctgaa agaacttaac gcgcaaacct ggtttgctgg cctgcgccgc
2281 gaacaatccg gcagccgcgc caatttaccg gtgctggcaa ttcagcgtgg cgtatttaaa
2341 gtgctgccga ttatcgactg ggataaccga actatttatc agtacctgca aaaacatggc
2401 ctgaaatatc acccattatg ggatgaagga tatttatcgg tcggtgatac ccatacaacc
2461 cgtaaatggg aacctggcat gtcggaagaa gaaacacgtt tctttggctt aaaaagggaa
2521 tgtggattgc acgaagggta aatattgccg gataaattaa acgcctctgt cagaaatgat
2581 gggggcgttt ttattttgcg agcagtattc ctgattttaa acgtgaacgc cgctggaaga
2641 attatcaggt atagaatggc gtcgggtgct tgaggctgtc tggcgttaag cattcgcgag
2701 gttccagatc cacaaaagcc ccaggcgata tttctatcaa cctaggctta cgttcgaacc
2761 caaacaattc gaatgttagt ctcttctttg cagggaggca agacatgctg acaaaatatg
2821 cccttgtggc aatatagtgc tgtgttttac agtactggga ttcacgctgc atggtaggcc
2881 actcgttgtg tgagttgagt atcagagaac gtggtatgga gtttaaggca gttctcgctt
2941 acgaatcgaa gaagtagccc accacgcggg gtaaaaccca taagcgctaa cttaagggtt
3001 gaaccatctg aagaatgcga tgcctcggtg cctcgttaag acgatgcctc gcgttcttaa
3061 ttgcgttttg taggctgtca gggatactgt cccacgaatg gccacctgta agctccaaga
3121 tgaccatttt tgttattctc cacaacgagt tagttcttct tttcggatcc ggcattctgg
3181 gggggaaatc cagcgaatgg ctggattatg tcgtcaattt tttgcggtga gtagtaatgc
3241 aaatatccag ctttcgcgag ttcaggttct ttgctcgcaa agcatccagg tgctgcaaac
3301 ttttgagccg cttaaaaagc cagttcaatt tgccatcgca gacggtaaca atcagccact
3361 tgctctgctg aatattcatc ttccggtaat gatgttagca atagcacatg gcccgctgct
3421 tccagcgttt ccgcctgaac tactcgtcct tttcgacgat tctcgctgag cagtcgggtt
3481 ttactgatta atgctttttc gggaggaagt gatacggcaa tgagacgtgc cggaaaggga
3541 gctccggctt tttattacct gaattgccta tcattacagt ggtttcaccg ttgttaccgc
3601 aatccagccc gcgcagaaaa cccatcatgt caaagcgcat tccttctgca g
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