GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS MUSIGAA302 384 bp ds-DNA ROD 10-MAY-1991
DEFINITION Mouse Ig H chain (subgroup I) pseudogene VGAM3-0 V/DFL6.1/J2
region.
ACCESSION X03090
KEYWORDS diversity region; immunoglobulin;
immunoglobulin heavy chain subgroup I; joining region; pseudogene;
variable region.
SEGMENT 2 of 2
SOURCE Mouse (strain C56BL/6 x BALB/c)F1 anti-bacterial lipopolysaccharide
B-lymphocyte DNA, clone GAM3-0-b.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 384)
AUTHORS Winter,E., Radbruch,A. and Krawinkel,U.
TITLE Members of novel VH gene families are found in VDJ regions of
polyclonally activated B-lymphocytes
JOURNAL EMBO J. 4, 2861-2867 (1985)
STANDARD simple automatic
COMMENT library=lambda L47.1; VGAM3-0 contains a VH segment belonging to
the J558 family and is derived from the Ighb locus. VGAM3-0 could
represent a rearranged pseudogene
EMBL features not translated to GenBank features:
key from to description
SITE 114 131 CDR1
SITE 174 227 CDR2
FEATURES Location/Qualifiers
CDS <17..>384
/partial
/pseudo
/note="pseudo-Ig H-chain precursor V/DFL6.1/J2-region (AA
at 19)"
sig_peptide <17..26
/partial
/pseudo
/note="pseudo-Ig H-chain signal peptide"
mat_peptide 27..>384
/pseudo
/note="pseudo-Ig H-chain"
misc_recomb 320^321
/note="V-regionend/N-region start"
misc_recomb 327^328
/note="N-region end/DSP2.2-region start"
misc_recomb 339^340
/note="DSP2.2-region end/N-region start"
misc_recomb 342^343
/note="N-region end/J2 region start"
BASE COUNT 99 a 98 c 101 g 86 t
ORIGIN About 300 bp after segment 1; chromosome 12.
1 ctgttctctc cacaggggtc tactcagagg ttcagctcca gcagtctggg actgtgctgg
61 caaggcctgg ggcttcagtg aagatgtcct gcaagacttc tggctacaca tttaccagct
121 actggatgca ctgggtaaaa cagaggcctg gacagggtct ggaatggata ggggctattt
181 atcctggaaa tagtgatact agctacaacc agaagttcaa gggcaaggcc aaactgactg
241 cagtcacatc cgccagcact gcctacatgg agctcagcag cctgacaaat gaggactctg
301 cggtctatta ctgtacaaga taagccttat gattacgacg tagactactg gggccaaggc
361 accactctca cagtctcctc aggt
//