GenBank-Updates@genbank.bio.net (11/22/90)
LOCUS VCHIRGA 1530 bp ds-DNA BCT 22-NOV-1990
DEFINITION V.cholerae IrgA (igrA) gene,.
ACCESSION M37773
KEYWORDS iron-regulated protein; transcriptional regulation.
SOURCE V.cholerae (strain 0395) DNA.
ORGANISM Vibrio cholerae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 1530)
AUTHORS Goldberg,M.B., Boyko,S.A. and Calderwood,S.B.
TITLE Transcriptional regulation by iron of a virulence gene of Vibrio
cholerae, and its homology to the Escherichia coli fur system
JOURNAL Unpublished (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence kindly submitted by
S.B.Calderwood, 16-AUG-1990.
Infect Disease Unit Mass General Hosp Fruit Street Boston, MA
02114
FEATURES from to/span description
pept 1077 > 1529 iron-regulated protein
mRNA 1041 1530 IrgA mRNA
signal 1027 1032 /new_qualifier="-10_signal"
signal 1066 1070 Shine Dalgarno /new_qualifier="RBS"
site 1529 1530 Vibrio cholerae/TnphoA junction
BASE COUNT 393 a 361 c 394 g 382 t
ORIGIN
1 atcgatgata aaaaatcccg ctgcggcggg attttttatt gccactcatc gggccttgct
61 tggcggagcg catcaataaa taggcgcagc cgaagtgggt gacgaccgag cggatagaag
121 cagttgattt ctgttggctg tgattgccat ccgttgacgc aaggaatgag gctgcccgga
181 tgcgccgttt caaaaccatt ggcaaaccaa gtgggaagca aaccaatacc acgaccttta
241 gcaatcgcat cggcttgcat ggcaagatta tcgctttgta aacgactctc tagtgctggc
301 agtgaataac tgccgaactc tggatggtgc agttcaagct ccgcgcgccg acaagcaata
361 aaatcaatcc atgggtgatg aatcagctca cgaggatggg tcggtttatc tcgatgggcc
421 aaatatttgg gagaggcgta agtggcatag cgccaatagc ctaagcgttc tttgcgataa
481 cccatggggg cggcgtgttc aatccaaatg atcaaatcgg gctcaaacac ctcatcactg
541 tgttgaaact ggctgagtag acggatcttc aatgtcgaat gctgctgcat aaactcatcc
601 aatacttggc tgagccagcc gcggatcaaa ttggggtgta ccaccagcgt gagttcgcca
661 gtcacttgat tgttcaattc ttgcaacgct tcctgacttt tattggccag ttcaagtagt
721 tgctccgagt aaaccgcaaa cacttctcct gctttggtga gcgttaagcg gttgccttga
781 cgcatcaaca agctttgtcc caagtcctct tcaagttgcg ccaaacggcg actcagggtg
841 gatttaggct gttcaagcgc tttggcagcg gcagtcaggc tcttatgttg gcaaagcgca
901 tggaaagctt ttacggcgct gagatcttgc ataggtattt gacccttaaa gaataattac
961 cacagacgtt ccatatttgg accgaactat tccatgtgtc gatctatctc cagtacagaa
1021 tatatgaata atccgcttct gaaattaaga ataattatca tttaaaggag tggtaaatgt
1081 ccagattcaa tccatccccc gtcagtttat ctgtgacact aggcttaatg ttttcggcta
1141 gcgcttttgc tcaagacgcg acgaaaacgg atgaaaccat ggtggtcact gcggcgggat
1201 acgcgcaagt gattcaaaat gcaccagcca gtatcagtgt gatttcaaga gaagatctgg
1261 aatctcgcta ttaccgtgat gtgaccgatg cgctaaaaag cgtaccgggt gtgacagtca
1321 ccggaggggg cgatactacc gatatcagca ttcgtggtat gggatcaaac tatactctta
1381 tcttggtgga tggtaagcgc caaacctcac gccagacccg tccaaacagc gatggcccgg
1441 gcattgagca aggttggtta ccgccactgc aagcgattga acgtatcgag gtgatccgtg
1501 gcccgatgtc tacgctgtac ggctcggatg
//GenBank-Updates@genbank.bio.net (01/17/91)
LOCUS VCHIRGA 1530 bp ds-DNA BCT 17-JAN-1991
DEFINITION V.cholerae IrgA (igrA) gene,.
ACCESSION M37773
KEYWORDS iron-regulated protein; transcriptional regulation.
SOURCE V.cholerae (strain 0395) DNA.
ORGANISM Vibrio cholerae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 1530)
AUTHORS Goldberg,M.B., Boyko,S.A. and Calderwood,S.B.
TITLE Transcriptional regulation by iron of a Vibrio cholerae virulence
gene and homology of the gene to the Escherichia coli Fur system
JOURNAL J. Bacteriol. 172, 6863-6870 (1991)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence kindly submitted
by S.B.Calderwood, 16-AUG-1990.
Infect Disease Unit
Mass General Hosp
Fruit Street
Boston, MA 02114
FEATURES Location/Qualifiers
-10_signal 1027..1032
/gene="irgA"
mRNA (1040.1041)..>1530
/gene="irgA"
RBS 1066..1070
/note="Shine Dalgarno"
/gene="irgA"
CDS 1077..>1529
/gene="irgA"
/codon_start=1077
/product="IrgA protein"
misc_feature 1529..1530
/note="Vibrio cholerae/TnphoA junction"
/gene="irgA"
BASE COUNT 393 a 361 c 394 g 382 t
ORIGIN
1 atcgatgata aaaaatcccg ctgcggcggg attttttatt gccactcatc gggccttgct
61 tggcggagcg catcaataaa taggcgcagc cgaagtgggt gacgaccgag cggatagaag
121 cagttgattt ctgttggctg tgattgccat ccgttgacgc aaggaatgag gctgcccgga
181 tgcgccgttt caaaaccatt ggcaaaccaa gtgggaagca aaccaatacc acgaccttta
241 gcaatcgcat cggcttgcat ggcaagatta tcgctttgta aacgactctc tagtgctggc
301 agtgaataac tgccgaactc tggatggtgc agttcaagct ccgcgcgccg acaagcaata
361 aaatcaatcc atgggtgatg aatcagctca cgaggatggg tcggtttatc tcgatgggcc
421 aaatatttgg gagaggcgta agtggcatag cgccaatagc ctaagcgttc tttgcgataa
481 cccatggggg cggcgtgttc aatccaaatg atcaaatcgg gctcaaacac ctcatcactg
541 tgttgaaact ggctgagtag acggatcttc aatgtcgaat gctgctgcat aaactcatcc
601 aatacttggc tgagccagcc gcggatcaaa ttggggtgta ccaccagcgt gagttcgcca
661 gtcacttgat tgttcaattc ttgcaacgct tcctgacttt tattggccag ttcaagtagt
721 tgctccgagt aaaccgcaaa cacttctcct gctttggtga gcgttaagcg gttgccttga
781 cgcatcaaca agctttgtcc caagtcctct tcaagttgcg ccaaacggcg actcagggtg
841 gatttaggct gttcaagcgc tttggcagcg gcagtcaggc tcttatgttg gcaaagcgca
901 tggaaagctt ttacggcgct gagatcttgc ataggtattt gacccttaaa gaataattac
961 cacagacgtt ccatatttgg accgaactat tccatgtgtc gatctatctc cagtacagaa
1021 tatatgaata atccgcttct gaaattaaga ataattatca tttaaaggag tggtaaatgt
1081 ccagattcaa tccatccccc gtcagtttat ctgtgacact aggcttaatg ttttcggcta
1141 gcgcttttgc tcaagacgcg acgaaaacgg atgaaaccat ggtggtcact gcggcgggat
1201 acgcgcaagt gattcaaaat gcaccagcca gtatcagtgt gatttcaaga gaagatctgg
1261 aatctcgcta ttaccgtgat gtgaccgatg cgctaaaaag cgtaccgggt gtgacagtca
1321 ccggaggggg cgatactacc gatatcagca ttcgtggtat gggatcaaac tatactctta
1381 tcttggtgga tggtaagcgc caaacctcac gccagacccg tccaaacagc gatggcccgg
1441 gcattgagca aggttggtta ccgccactgc aagcgattga acgtatcgag gtgatccgtg
1501 gcccgatgtc tacgctgtac ggctcggatg
//GenBank-Updates@genbank.bio.net (01/31/91)
LOCUS VCHIRGA 1530 bp ds-DNA BCT 31-JAN-1991
DEFINITION V.cholerae IrgA (igrA) gene,.
ACCESSION M37773
KEYWORDS iron-regulated protein; transcriptional regulation.
SOURCE V.cholerae (strain 0395) DNA.
ORGANISM Vibrio cholerae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 1530)
AUTHORS Goldberg,M.B., Boyko,S.A. and Calderwood,S.B.
TITLE Transcriptional regulation by iron of a Vibrio cholerae virulence
gene and homology of the gene to the Escherichia coli Fur system
JOURNAL J. Bacteriol. 172, 6863-6870 (1991)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence kindly submitted
by S.B.Calderwood, 16-AUG-1990.
Infect Disease Unit
Mass General Hosp
Fruit Street
Boston, MA 02114
FEATURES Location/Qualifiers
-10_signal 1027..1032
/gene="irgA"
mRNA (1040.1041)..>1530
/gene="irgA"
RBS 1066..1070
/note="Shine Dalgarno"
/gene="irgA"
CDS 1077..>1529
/product="IrgA protein"
/gene="irgA"
/codon_start=1077
misc_feature 1529..1530
/note="Vibrio cholerae/TnphoA junction"
/gene="irgA"
BASE COUNT 393 a 361 c 394 g 382 t
ORIGIN
1 atcgatgata aaaaatcccg ctgcggcggg attttttatt gccactcatc gggccttgct
61 tggcggagcg catcaataaa taggcgcagc cgaagtgggt gacgaccgag cggatagaag
121 cagttgattt ctgttggctg tgattgccat ccgttgacgc aaggaatgag gctgcccgga
181 tgcgccgttt caaaaccatt ggcaaaccaa gtgggaagca aaccaatacc acgaccttta
241 gcaatcgcat cggcttgcat ggcaagatta tcgctttgta aacgactctc tagtgctggc
301 agtgaataac tgccgaactc tggatggtgc agttcaagct ccgcgcgccg acaagcaata
361 aaatcaatcc atgggtgatg aatcagctca cgaggatggg tcggtttatc tcgatgggcc
421 aaatatttgg gagaggcgta agtggcatag cgccaatagc ctaagcgttc tttgcgataa
481 cccatggggg cggcgtgttc aatccaaatg atcaaatcgg gctcaaacac ctcatcactg
541 tgttgaaact ggctgagtag acggatcttc aatgtcgaat gctgctgcat aaactcatcc
601 aatacttggc tgagccagcc gcggatcaaa ttggggtgta ccaccagcgt gagttcgcca
661 gtcacttgat tgttcaattc ttgcaacgct tcctgacttt tattggccag ttcaagtagt
721 tgctccgagt aaaccgcaaa cacttctcct gctttggtga gcgttaagcg gttgccttga
781 cgcatcaaca agctttgtcc caagtcctct tcaagttgcg ccaaacggcg actcagggtg
841 gatttaggct gttcaagcgc tttggcagcg gcagtcaggc tcttatgttg gcaaagcgca
901 tggaaagctt ttacggcgct gagatcttgc ataggtattt gacccttaaa gaataattac
961 cacagacgtt ccatatttgg accgaactat tccatgtgtc gatctatctc cagtacagaa
1021 tatatgaata atccgcttct gaaattaaga ataattatca tttaaaggag tggtaaatgt
1081 ccagattcaa tccatccccc gtcagtttat ctgtgacact aggcttaatg ttttcggcta
1141 gcgcttttgc tcaagacgcg acgaaaacgg atgaaaccat ggtggtcact gcggcgggat
1201 acgcgcaagt gattcaaaat gcaccagcca gtatcagtgt gatttcaaga gaagatctgg
1261 aatctcgcta ttaccgtgat gtgaccgatg cgctaaaaag cgtaccgggt gtgacagtca
1321 ccggaggggg cgatactacc gatatcagca ttcgtggtat gggatcaaac tatactctta
1381 tcttggtgga tggtaagcgc caaacctcac gccagacccg tccaaacagc gatggcccgg
1441 gcattgagca aggttggtta ccgccactgc aagcgattga acgtatcgag gtgatccgtg
1501 gcccgatgtc tacgctgtac ggctcggatg
//GenBank-Updates@genbank.bio.net (04/10/91)
LOCUS VIBIRGA 1530 bp ds-DNA BCT 10-APR-1991
DEFINITION V.cholerae IrgA (igrA) gene, 5' end.
ACCESSION M37773
KEYWORDS iron-regulated protein; transcriptional regulation.
SOURCE V.cholerae (strain 0395) DNA.
ORGANISM Vibrio cholerae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 1530)
AUTHORS Goldberg,M.B., Boyko,S.A., Butterton,J.R., Stoebner,J.A.,
Payne,S.M. and Calderwood,S.B.
TITLE Nucleotide sequence of irgA, an iron-regulated virulence gene of
Vibrio cholerae, with homology to the Escherichia coli colicin I
receptor
JOURNAL Unpublished (1991)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1530)
AUTHORS Goldberg,M.B., Boyko,S.A. and Calderwood,S.B.
TITLE Transcriptional regulation by iron of a Vibrio cholerae virulence
gene and homology of the gene to the Escherichia coli Fur system
JOURNAL J. Bacteriol. 172, 6863-6870 (1991)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence kindly submitted
by S.B.Calderwood, 16-AUG-1990.
Infect Disease Unit
Mass General Hosp
Fruit Street
Boston, MA 02114
FEATURES Location/Qualifiers
-10_signal 1027..1032
/gene="irgA"
mRNA (1040.1041)..>1530
/gene="irgA"
RBS 1066..1070
/note="Shine Dalgarno"
/gene="irgA"
CDS 1077..>1529
/product="IrgA protein"
/gene="irgA"
/codon_start=1077
misc_feature 1529..1530
/note="Vibrio cholerae/TnphoA junction"
/gene="irgA"
BASE COUNT 393 a 361 c 394 g 382 t
ORIGIN
1 atcgatgata aaaaatcccg ctgcggcggg attttttatt gccactcatc gggccttgct
61 tggcggagcg catcaataaa taggcgcagc cgaagtgggt gacgaccgag cggatagaag
121 cagttgattt ctgttggctg tgattgccat ccgttgacgc aaggaatgag gctgcccgga
181 tgcgccgttt caaaaccatt ggcaaaccaa gtgggaagca aaccaatacc acgaccttta
241 gcaatcgcat cggcttgcat ggcaagatta tcgctttgta aacgactctc tagtgctggc
301 agtgaataac tgccgaactc tggatggtgc agttcaagct ccgcgcgccg acaagcaata
361 aaatcaatcc atgggtgatg aatcagctca cgaggatggg tcggtttatc tcgatgggcc
421 aaatatttgg gagaggcgta agtggcatag cgccaatagc ctaagcgttc tttgcgataa
481 cccatggggg cggcgtgttc aatccaaatg atcaaatcgg gctcaaacac ctcatcactg
541 tgttgaaact ggctgagtag acggatcttc aatgtcgaat gctgctgcat aaactcatcc
601 aatacttggc tgagccagcc gcggatcaaa ttggggtgta ccaccagcgt gagttcgcca
661 gtcacttgat tgttcaattc ttgcaacgct tcctgacttt tattggccag ttcaagtagt
721 tgctccgagt aaaccgcaaa cacttctcct gctttggtga gcgttaagcg gttgccttga
781 cgcatcaaca agctttgtcc caagtcctct tcaagttgcg ccaaacggcg actcagggtg
841 gatttaggct gttcaagcgc tttggcagcg gcagtcaggc tcttatgttg gcaaagcgca
901 tggaaagctt ttacggcgct gagatcttgc ataggtattt gacccttaaa gaataattac
961 cacagacgtt ccatatttgg accgaactat tccatgtgtc gatctatctc cagtacagaa
1021 tatatgaata atccgcttct gaaattaaga ataattatca tttaaaggag tggtaaatgt
1081 ccagattcaa tccatccccc gtcagtttat ctgtgacact aggcttaatg ttttcggcta
1141 gcgcttttgc tcaagacgcg acgaaaacgg atgaaaccat ggtggtcact gcggcgggat
1201 acgcgcaagt gattcaaaat gcaccagcca gtatcagtgt gatttcaaga gaagatctgg
1261 aatctcgcta ttaccgtgat gtgaccgatg cgctaaaaag cgtaccgggt gtgacagtca
1321 ccggaggggg cgatactacc gatatcagca ttcgtggtat gggatcaaac tatactctta
1381 tcttggtgga tggtaagcgc caaacctcac gccagacccg tccaaacagc gatggcccgg
1441 gcattgagca aggttggtta ccgccactgc aagcgattga acgtatcgag gtgatccgtg
1501 gcccgatgtc tacgctgtac ggctcggatg
//GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS VIBIRGA 1530 bp ds-DNA BCT 10-MAY-1991
DEFINITION V.cholerae IrgA (igrA) gene, 5' end.
ACCESSION M37773
KEYWORDS iron-regulated protein; transcriptional regulation.
SOURCE V.cholerae (strain 0395) DNA.
ORGANISM Vibrio cholerae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 1530)
AUTHORS Goldberg,M.B., Boyko,S.A., Butterton,J.R., Stoebner,J.A.,
Payne,S.M. and Calderwood,S.B.
TITLE Nucleotide sequence of irgA, an iron-regulated virulence gene of
Vibrio cholerae, with homology to the Escherichia coli colicin I
receptor
JOURNAL Unpublished (1991)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1530)
AUTHORS Goldberg,M.B., Boyko,S.A. and Calderwood,S.B.
TITLE Transcriptional regulation by iron of a Vibrio cholerae virulence
gene and homology of the gene to the Escherichia coli Fur system
JOURNAL J. Bacteriol. 172, 6863-6870 (1991)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence kindly submitted
by S.B.Calderwood, 16-AUG-1990.
Infect Disease Unit
Mass General Hosp
Fruit Street
Boston, MA 02114
FEATURES Location/Qualifiers
-10_signal 1027..1032
/gene="irgA"
mRNA (1040.1041)..>1530
/gene="irgA"
RBS 1066..1070
/note="Shine Dalgarno"
/gene="irgA"
CDS 1077..>1529
/product="IrgA protein"
/gene="irgA"
/codon_start=1077
misc_feature 1529..1530
/note="Vibrio cholerae/TnphoA junction"
/gene="irgA"
BASE COUNT 393 a 361 c 394 g 382 t
ORIGIN
1 atcgatgata aaaaatcccg ctgcggcggg attttttatt gccactcatc gggccttgct
61 tggcggagcg catcaataaa taggcgcagc cgaagtgggt gacgaccgag cggatagaag
121 cagttgattt ctgttggctg tgattgccat ccgttgacgc aaggaatgag gctgcccgga
181 tgcgccgttt caaaaccatt ggcaaaccaa gtgggaagca aaccaatacc acgaccttta
241 gcaatcgcat cggcttgcat ggcaagatta tcgctttgta aacgactctc tagtgctggc
301 agtgaataac tgccgaactc tggatggtgc agttcaagct ccgcgcgccg acaagcaata
361 aaatcaatcc atgggtgatg aatcagctca cgaggatggg tcggtttatc tcgatgggcc
421 aaatatttgg gagaggcgta agtggcatag cgccaatagc ctaagcgttc tttgcgataa
481 cccatggggg cggcgtgttc aatccaaatg atcaaatcgg gctcaaacac ctcatcactg
541 tgttgaaact ggctgagtag acggatcttc aatgtcgaat gctgctgcat aaactcatcc
601 aatacttggc tgagccagcc gcggatcaaa ttggggtgta ccaccagcgt gagttcgcca
661 gtcacttgat tgttcaattc ttgcaacgct tcctgacttt tattggccag ttcaagtagt
721 tgctccgagt aaaccgcaaa cacttctcct gctttggtga gcgttaagcg gttgccttga
781 cgcatcaaca agctttgtcc caagtcctct tcaagttgcg ccaaacggcg actcagggtg
841 gatttaggct gttcaagcgc tttggcagcg gcagtcaggc tcttatgttg gcaaagcgca
901 tggaaagctt ttacggcgct gagatcttgc ataggtattt gacccttaaa gaataattac
961 cacagacgtt ccatatttgg accgaactat tccatgtgtc gatctatctc cagtacagaa
1021 tatatgaata atccgcttct gaaattaaga ataattatca tttaaaggag tggtaaatgt
1081 ccagattcaa tccatccccc gtcagtttat ctgtgacact aggcttaatg ttttcggcta
1141 gcgcttttgc tcaagacgcg acgaaaacgg atgaaaccat ggtggtcact gcggcgggat
1201 acgcgcaagt gattcaaaat gcaccagcca gtatcagtgt gatttcaaga gaagatctgg
1261 aatctcgcta ttaccgtgat gtgaccgatg cgctaaaaag cgtaccgggt gtgacagtca
1321 ccggaggggg cgatactacc gatatcagca ttcgtggtat gggatcaaac tatactctta
1381 tcttggtgga tggtaagcgc caaacctcac gccagacccg tccaaacagc gatggcccgg
1441 gcattgagca aggttggtta ccgccactgc aagcgattga acgtatcgag gtgatccgtg
1501 gcccgatgtc tacgctgtac ggctcggatg
//