GenBank-Updates@genbank.bio.net (09/29/90)
LOCUS NEURRNA1 614 bp ss-rRNA RNA 29-SEP-1990
DEFINITION N.crassa (isolate N4317) 28S-like ribosomal RNA, 5' end.
ACCESSION M38154
KEYWORDS 28S ribosomal RNA; 28S-like ribosomal RNA.
SEGMENT 1 of 2
SOURCE N.crassa (isolate N 4317), cDNA to rRNA.
ORGANISM Neurospora crassa
Eukaryota; Plantae; Thallobionta; Eumycota; Ascomycotina;
Pyrenomycetes; Sordariales; Sordariaceae.
REFERENCE 1 (bases 1 to 614)
AUTHORS Guadet,J., Julien,J., Lafay,J.F. and Brygoo,Y.
TITLE Phylogeny of some Fusarium species, as determined by large-subunit
rRNA sequence comparison
JOURNAL Mol. Biol. Evol. 6, 227-242 (1989)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by J.F.Lafay, 28-AUG-1990.
FEATURES from to/span description
rRNA 1 614 28S-like ribosomal RNA
BASE COUNT 166 a 133 c 180 g 135 t
ORIGIN
1 ttgacctcgg atcaggtagg aatacccgct gaacttaagc atatcaataa gcggaggaaa
61 agaaaccaac agggattgcc ctagtaacgg cgagtgaagc ggcaacagct caaatttgaa
121 atctggcttc ggcccgagtt gtaatttgta gaggaagctt ttggtgaggc accttctgag
181 tcccctggaa cggggcgcca tagagggtga gagccccgta tagtcggctg ccgatccaat
241 gtaaagctcc ttcgacgagt cgagtagttt gggaatgctg ctcaaaatgg gaggtaaatt
301 tcttctaaag ctaaatattg gccagagacc gatagcgcac aagtagagtg atcgaaagat
361 gaaaagcact ttgaaaagag agtcaaaaag tacgtgaaat tgttgaaagg gaagcgtttg
421 tgaccagact tcgccttcca tcatcatgtg ctgttctcac cggtgcactc ggacagctca
481 ggccagcatc ggttttggcg ggggataaag gtccggggaa cgtagctctc cgggagtgtt
541 atagcccggc gtaatgcctc gccgggaccg aggttcgcgc atctgcaagg atgctggcgt
601 aatggtcatc aacg
//GenBank-Updates@genbank.bio.net (05/07/91)
LOCUS NEUTUBB 2585 bp ds-DNA PLN 07-MAY-1991
DEFINITION N.crassa (benomyl-resistant mutant) beta-tubulin gene, complete
cds.
ACCESSION M13630
KEYWORDS benomyl resistance; beta-tubulin; tubulin.
SOURCE N.crassa (benomyl resistant mutant Bml511(r)a) DNA, clone pBT3.
ORGANISM Neurospora crassa
Eukaryota; Plantae; Thallobionta; Eumycota; Ascomycotina;
Pyrenomycetes; Sordariales; Sordariaceae.
REFERENCE 1 (bases 1 to 2585)
AUTHORS Orbach,M.J., Porro,E.B. and Yanofsky,C.
TITLE Cloning and characterization of the gene for beta-tubulin from a
benomyl-resistant mutant of Neurospora crassa and its use as a
dominant selectable marker
JOURNAL Mol. Cell. Biol. 6, 2452-2461 (1986)
STANDARD full staff_review
COMMENT Draft entry and sequence in computer-readable form for [Mol. Cell.
Biol. 6, 2452-2461 (1986)] kindly
provided by M.J.Orbach, 08-DEC-1986.
The N.crassa genome contains only one beta-tubulin gene. The
change at position 1378 to an 'a' in the benomyl-resistant strain
of N.crassa results in the substitution of a tyrosine for a
phenylalanine (found in the wild type).
FEATURES Location/Qualifiers
prim_transcript <359..2546
/note="btub mRNA (alt.)"
prim_transcript <359..2547
/note="btub mRNA (alt.)"
CDS join(359..370,611..634,709..734,803..844,910..964,
1038..1828,1886..2279)
/note="beta-tubulin"
/codon_start=359
exon 359..370
/note="beta-tubulin, exon 1"
intron 371..610
/note="btub intron A"
exon 611..634
/note="beta-tubulin, exon 2"
intron 635..708
/note="btub intron B"
exon 709..734
/note="beta-tubulin, exon 3"
intron 735..802
/note="btub intron C"
exon 803..844
/note="beta-tubulin, exon 4"
intron 845..909
/note="btub intron D"
exon 910..964
/note="beta-tubulin, exon 5"
intron 965..1037
/note="btub intron E"
exon 1038..1828
/note="beta-tubulin, exon 6"
mutation 1378..1378
/note="a in Bml511(r)a; t in wt"
intron 1829..1885
/note="btub intron F"
exon 1886..2279
/note="beta-tubulin, exon 7"
BASE COUNT 533 a 846 c 591 g 615 t
ORIGIN 1 bp upstream of SalI site; linkage group VI L.
1 gtcgacaggg ggccttccac ccttccaaaa gtttgacagt gcctgatgtc cccactttgg
61 caagcgcccc ggcaacccag cagcagcccg gcgagcggcg gcggcggtga acgagaagct
121 ggtgctggcc ggctgtggcc cgctcatttc ttgcaccaaa aatcaccaaa tctccctttc
181 cactcccaag acttcccagt ctcccctagg ccagacatca aacctctcca ctttcaccga
241 cctccgtcgt cttcgtctat ccgcgtcggc tttatcacca cttccatctt tttgctcttc
301 catctttctg ccttcaacct ccacatagac ccgtgttcgt catcaccaaa ccgtcaagat
361 gcgtgaaatt gtaagtctcc ctctgatgcc tactgtgccg cgcagcagag caatccctct
421 tttttttctc tttccctccc aagagacccc tgattgctct accccaccaa actgcctcgt
481 ccccttctgt gcctctacct actgctgccc gactcattat gatcctgctc gctttctcgc
541 catcgcggac tgccatctat cgcgagtatt atggacatcg acgctgacac gatttcttca
601 cgtgctatag gttcatctcc aaaccggcca atgcgtaagt cgatgatggc ttccaccccc
661 gtcgcgatcc aacaacaaca gcaggactaa cacaacagcc acgatcaggg taaccaaatc
721 ggtgctgctt tctggtacgt cgtcatcatc gcgaacaacc acacctttga gcgagggagc
781 ccgacgctga cagaataaac aggcagacta tctccggcga gcacggcctc gatgcctccg
841 gtgtgtacgt aacctcgtgc ccaataacca tatctggtga tggcctcgaa agctcaccgc
901 ccactacagg tacaatggca cctctgagct ccagctcgag cgcatgaacg tctacttcaa
961 cgaggtgagc aacaaccggt ctcgacgatc tccccctcct cgagaattcg ctcgctaact
1021 agcttcgcgc ttgacaggct tccggcaaca agtatgtccc tcgtgccgtc ctcgtcgatc
1081 tcgagcccgg taccatggac gccgttcgcg ccggtccctt cggccagctc ttccgccccg
1141 ataacttcgt cttcggccag tccggtgctg gcaacaactg ggccaagggt cactacactg
1201 agggtgctga gcttgttgac caggttctcg atgtcgtccg tcgcgaggct gagggctgcg
1261 actgcctcca gggcttccag atcacccact ccctcggtgg tggtaccggt gccggtatgg
1321 gtaccctcct tatctccaag attcgtgagg agttccccga ccgcatgatg gctacctact
1381 ccgtcgtgcc ctcccccaag gtctccgata ccgttgtcga gccctacaac gccaccctct
1441 ccgtccatca gctcgttgag aactccgacg agaccttctg cattgacaac gaggcgcttt
1501 acgacatttg catgaggacc ctcaagctct ccaacccctc ttacggcgat cttaaccacc
1561 tcgtctccgc cgtcatgtcc ggtgtcaccg tctccctccg tttccccggc cagctgaact
1621 ccgatctccg caagctcgcc gtcaacatgg ttcccttccc ccgtctccac ttcttcatgg
1681 tcgggttcgc tccccttacc agccgcggcg cccaccattt ccgtgccgtc tccgtgcccg
1741 agttgaccca gcagatgttc gaccccaaga acatgatggc tgcttctgac ttccgcaacg
1801 gtcgttacct cacctgctct gccatcttgt aagttgctga tcatggaatg ctgcttttga
1861 ccactaatac tgacgaatca aacagccgtg gcaaggtctc catgaaggag gttgaggacc
1921 agatgcgcaa cgttcagaac aagaactctt cctacttcgt cgagtggatc cccaacaacg
1981 tccagactgc cctctgctct atccctcccc gcggtctcaa gatgtcctcc accttcgtcg
2041 gtaactccac cgccatccag gagctcttca agcgtatcgg cgagcagttc actgccatgt
2101 tcaagcgcaa ggctttcttg cattggtaca ctggtgaggg tatggacgag atggagttca
2161 ctgaggctga gtccaacatg aacgatctcg tctccgagta ccagcagtac caggatgctg
2221 gtgttgacga ggaggaggag gagtacgagg aggaggcccc ccttgagggc gaggagtaaa
2281 tcattccact caacattcag gctcctctgc gcacgtaaag tccaaaggca ataccctgct
2341 cggtggaatg cgccgggctt gtcgatttta cgcacatatg cgcattcttg acttgaagcg
2401 gaggagttct tcgttgcggg ttacagtgtt ttaataaaag aatggtcaaa tcaaactgct
2461 agatatacct gtcagacact ctagttgttg acccctatac tcttaataca tcagacagta
2521 catgcatgtt gcatgatgat gataatgtct gtttagattc caagtgtcta ctgctggcca
2581 agctt
//GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS NEURRNA 614 bp ss-rRNA RNA 10-MAY-1991
DEFINITION N.crassa (isolate N4317) 28S-like ribosomal RNA, 5' end.
ACCESSION M38154
KEYWORDS 28S ribosomal RNA; 28S-like ribosomal RNA.
SOURCE N.crassa (isolate N 4317), cDNA to rRNA.
ORGANISM Neurospora crassa
Eukaryota; Plantae; Thallobionta; Eumycota; Ascomycotina;
Pyrenomycetes; Sordariales; Sordariaceae.
REFERENCE 1 (bases 1 to 614)
AUTHORS Guadet,J., Julien,J., Lafay,J.F. and Brygoo,Y.
TITLE Phylogeny of some Fusarium species, as determined by large-subunit
rRNA sequence comparison
JOURNAL Mol. Biol. Evol. 6, 227-242 (1989)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Mol. Biol. Evol. 6,
227-242 (1989)] kindly submitted
by J.F.Lafay, 28-AUG-1990.
FEATURES Location/Qualifiers
rRNA 1..>614
/product="28S-like ribosomal RNA"
BASE COUNT 166 a 133 c 180 g 135 t
ORIGIN
1 ttgacctcgg atcaggtagg aatacccgct gaacttaagc atatcaataa gcggaggaaa
61 agaaaccaac agggattgcc ctagtaacgg cgagtgaagc ggcaacagct caaatttgaa
121 atctggcttc ggcccgagtt gtaatttgta gaggaagctt ttggtgaggc accttctgag
181 tcccctggaa cggggcgcca tagagggtga gagccccgta tagtcggctg ccgatccaat
241 gtaaagctcc ttcgacgagt cgagtagttt gggaatgctg ctcaaaatgg gaggtaaatt
301 tcttctaaag ctaaatattg gccagagacc gatagcgcac aagtagagtg atcgaaagat
361 gaaaagcact ttgaaaagag agtcaaaaag tacgtgaaat tgttgaaagg gaagcgtttg
421 tgaccagact tcgccttcca tcatcatgtg ctgttctcac cggtgcactc ggacagctca
481 ggccagcatc ggttttggcg ggggataaag gtccggggaa cgtagctctc cgggagtgtt
541 atagcccggc gtaatgcctc gccgggaccg aggttcgcgc atctgcaagg atgctggcgt
601 aatggtcatc aacg
//