GenBank-Updates@genbank.bio.net (02/02/91)
LOCUS BUTXYLB 4197 bp ds-DNA BCT 02-FEB-1991
DEFINITION B.fibrisolvens beta-D-xylosidase/alpha-L-arabinofuranosidase gene,
complete cds.
ACCESSION M55537
KEYWORDS alpha-L-arabinofuranosidase; beta-D-xylosidase; beta-xylosidase.
SOURCE B.fibrisolvens DNA.
ORGANISM Butyrivibrio fibrisolvens
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Anaerobic
gram-negative straight, curved and helical rods; Bacteroidaceae.
REFERENCE 1 (bases 1 to 4197)
AUTHORS Utt,E.A., Eddy,C.K., Keshav,K.F. and Ingram,L.O.
TITLE Sequencing and expression of the Butyrivibrio fibrisolvens xylB
gene encoding a novel bifunctional protein with beta-D-Xylosidase
and alpha-L-Arabinofuranosidase activities
JOURNAL Unpublished (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS <1..1339
/label=ORF1
CDS 1353..2906
/number=3.2.1.37
/number=3.2.1.55
/gene="xylB"
/codon_start=1353
/product="beta-D-xylosidase/alpha-L-arabinofuranosi
dase"
CDS 3030..4197
/label=ORF3
/codon_start=3030
/partial
BASE COUNT 1350 a 666 c 944 g 1237 t
ORIGIN
1 aattgtggat gcacatatga aaagctgatt tatgcttata aggcaggtct tgtcaaggaa
61 gagaccatcg atgaggctgt tactcgactt atggaaatca gacttcgtct aggtactatt
121 ccagagagaa agagtaagta tgatgatatc ccatatgaag tggtcgaatg caaagagcat
181 atcaaacttg ctcttgacgc tgcaaaggat agctttgtcc ttttgaagaa tgatggttta
241 cttccactga ataaaaagga ttataaatct attgctgtta ttggacccaa ctctgattca
301 agaagagctt taattggaaa ttatgagggc ctttcttcag agtatattac agttttagag
361 gggattcgtc aggttgtcgg tgatgatatt agattattcc acgctgaggg cactcatctt
421 tggaaggata gaattcacgt aatcagtgag ccaaaagatg gatttgccga ggctaaaatc
481 gtggcagagc attcagattt agttgtgatg tgtcttggac ttgacgcatc aatcgaagga
541 gaagaaggag acgagggtaa tcagttcggt agcggagaca agcctggatt aaagcttaca
601 ggttgtcagc aagagctact tgaggaaatt gccaaaatcg gcaagcctgt tgtacttctt
661 gtgctttcag gttctgctct tgatttatca tgggcgcagg aatctaataa cgtaaatgcg
721 ataatgcagt gctggtatcc aggcgcaaga ggtggacgtg ctattgcaga ggttttattt
781 ggcaaggcca gtccaggcgg taaaatgcct cttacatttt atgcctcaga tgatgacctt
841 cctgattttt ctgattattc aatggaaaat aggacataca gatatttcaa gggcacacca
901 ctttatccat ttggttatgg actaggttat tctaaaattg attatctatt tgcttctatt
961 gataaagata agggagcaat tggtgataca ttcaagctaa aggtagatgt taaaaatacc
1021 ggtaagtata cacagcatga ggctgttcaa gtatatgtaa cggaccttga ggcaacgaca
1081 agagtgccta ttagaagcct tagaaaggtt aaatgtctag agcttgagcc tggtgaaaca
1141 aaagaggttg aatttaccct ttttgcaaga gattttgcca ttattgatga aaggggaaaa
1201 tgtatcatag agccaggcaa gtttaagatt tctattgggg gacaacagcc agacgataga
1261 agtaaagaac ttatgggcag agagtgtgat atttttgaaa ttgaattaac aggctctgtt
1321 acagaagttg aatattaatt gagaggtgca tcatggttat agctaacaat ccaattttaa
1381 aaggttttta tccagaccct tctatctgca gaaaagggga tgatttttat ctagtttgtt
1441 caagttttgt gtatgctccg ggagtaccga tttttcacac taaggatttg gcacattttg
1501 agcaaattgg aaatatatta gacagagaaa gtcaacttcc attgtcggga gatatatcta
1561 gaggcatatt tgccccaaca ataagagagc ataatggaat cttttacatg ataacaacta
1621 atgtaagctc tggcggcaac tttattgtta ctgcaaaaga tccagctggt ccttggtcag
1681 agccatatta tttaggtgaa gatgaggcgc caggtattga tccatctctg ttttttgatg
1741 acgatggcaa atgttattac gttggtacca gaccaaatcc tgatggagtt cgttacaacg
1801 gtgattggga gatatgggtt caagagctgg atttagagca aatgaaactt gtaggtcctt
1861 cgatggcaat ttggaagggc gctcttaagg atgttatttg gccagaagga ccacaccttt
1921 ataagaaaga tggatattat tatcttttac atgcagaagc tggcacaagc tttgaacatg
1981 ctatttctgt agctcgctca aaggagctat tcaaatggtt tgagggatgt cctagaaatc
2041 ctatatttac ccatagaaat ttaggcaagg attatccagt atgcaatgtt ggacatgctg
2101 atttagttga tgatatcaat ggcaactggt atatggtgat gctggcatct agaccatgca
2161 agggaaagtg cagcttggga cgagagacat tccttgcaaa agtaatttgg gaagacggat
2221 ggccagtggt taatccggga gttggtcgtt tgactgatga ggtggagatg gaccttcctg
2281 aatatagatt ctcaaaagag attactacaa aggataaaat gacctttgaa gagacagtcc
2341 tagatgatag atttgttgga attgaaagaa gaagtgagga cttttattcc cttactgaca
2401 atcctggatt cttaagatta aagcttcgtc ctgaggccat agaaaatact ggcaatccat
2461 cttacttagg aattcgtcaa aagactcatt cgtttagagc aagctgtggc cttaagttta
2521 caccagcaaa agataatgaa tgtgcaggaa tggtgttatt ccagaataat gaaaatcact
2581 tggagctttt agttgtaaag aagaaagata agctacagtt taaagtagga ccagttatta
2641 aaggaaccaa aatcagactt gctacttttg atatttcatc aggtgattta gaaattattc
2701 ttgaggcagc aaatcagctg gctaatatct atattaaaaa gaataatgaa aagattcttg
2761 tggcagaatg tattgatttg agcccataca ctaccgaaga atcaggcgga ttcgtaggat
2821 gtaccattgg actatatgct tcttcaaatg gaaagaccag tgataactat tgcgattatt
2881 cctactttac agtagaagaa gtatagcatt ttcaatgagc gaatttgcaa gttttatata
2941 cgggattaat tgtacgtaaa aaccatacag gtgtaaaata gtttccagag aaagtttttt
3001 ctctggaatt ttttattatg gaggggatta tgcttcagga aagtattaag aagttggtac
3061 agtacggtat tgatatgggg cttacaccag aatgtgagag aatatatact acaaatcttt
3121 tgcttgaatg tatgaaagaa gatgagtaca tagatccaga ctgtgattta agcaatatta
3181 tacttgaaga tgtattaaag gaacttttag atgaggcagt taatagaggt atcatagagg
3241 attcagttac acatagggat ttgtttgata caaagctaat gaatcagcta tgcccacgtc
3301 ctaaacaggt tatagatgat tttaaccgta tatacgataa ccatggtcca atagctgcaa
3361 cagattattt ttacaagtta agcaaagcct ctgactatat ccgtacttac agggtaaaaa
3421 aggacctaaa atggacatgc gatacagagt atggcactct tgacataaca attaatctct
3481 ctaagccaga aaaagaccca aaggcaattg ctgcagctaa gaatgcaaaa caatccacat
3541 atccgaagtg ccaattatgt atggaaaatg aaggctatgc tggtcgcatt aatcatcctg
3601 ctagagagaa tcatcgcata attcctataa ctataaataa cagcaactgg ggatttcaat
3661 atagcccata cgtttattac aatgagcatt gcatagtctt taacggagag catactccta
3721 tgaaaataga gcgagctact tttgttaagc tatttgattt tatcaaacta tttccacact
3781 attttttagg aagcaatgct gatttaccaa ttgttggagg atctatttta agccatgacc
3841 atttccaagg cggccattac acatttgcca tggaaaaagc tccaattatt caggaattta
3901 ctgtaaaagg atatgaggat gttaaggctg gtatagttaa atggccactt tcagtaatta
3961 gacttcagtg caaggatgag actagactta ttgatttagc gactaatata ttagacaaat
4021 ggagaaatta caccgatgaa gaggcatata tttttgctga aacagatggt gagcctcaca
4081 atacgattac acctattgct agaaaaagag gggattactt tgaactagat cctctagagt
4141 cgacctgcag gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactggg
//GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS BUTXYLB 4197 bp ds-DNA BCT 10-MAY-1991
DEFINITION B.fibrisolvens beta-D-xylosidase/alpha-L-arabinofuranosidase gene,
complete cds.
ACCESSION M55537
KEYWORDS alpha-L-arabinofuranosidase; beta-D-xylosidase; beta-xylosidase.
SOURCE B.fibrisolvens DNA.
ORGANISM Butyrivibrio fibrisolvens
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Anaerobic gram-negative straight, curved and helical rods;
Bacteroidaceae.
REFERENCE 1 (bases 1 to 4197)
AUTHORS Utt,E.A., Eddy,C.K., Keshav,K.F. and Ingram,L.O.
TITLE Sequencing and expression of the butyrivibrio fibrisolven xylb gene
encoding a novel bifunctional protein with beta-d-xylosidas and
alpha-l-arabinofuranosidase activities
JOURNAL Appl. Environ. Microbiol. 57, 1227-1234 (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS <1..1339
/label=ORF1
/codon_start=1
CDS 1353..2906
/number=3.2.1.37
/number=3.2.1.55
/product="beta-D-xylosidase/alpha-L-arabinofuranosidase"
/gene="xylB"
/codon_start=1353
CDS 3030..4197
/label=ORF3
/codon_start=3030
/partial
BASE COUNT 1350 a 666 c 944 g 1237 t
ORIGIN
1 aattgtggat gcacatatga aaagctgatt tatgcttata aggcaggtct tgtcaaggaa
61 gagaccatcg atgaggctgt tactcgactt atggaaatca gacttcgtct aggtactatt
121 ccagagagaa agagtaagta tgatgatatc ccatatgaag tggtcgaatg caaagagcat
181 atcaaacttg ctcttgacgc tgcaaaggat agctttgtcc ttttgaagaa tgatggttta
241 cttccactga ataaaaagga ttataaatct attgctgtta ttggacccaa ctctgattca
301 agaagagctt taattggaaa ttatgagggc ctttcttcag agtatattac agttttagag
361 gggattcgtc aggttgtcgg tgatgatatt agattattcc acgctgaggg cactcatctt
421 tggaaggata gaattcacgt aatcagtgag ccaaaagatg gatttgccga ggctaaaatc
481 gtggcagagc attcagattt agttgtgatg tgtcttggac ttgacgcatc aatcgaagga
541 gaagaaggag acgagggtaa tcagttcggt agcggagaca agcctggatt aaagcttaca
601 ggttgtcagc aagagctact tgaggaaatt gccaaaatcg gcaagcctgt tgtacttctt
661 gtgctttcag gttctgctct tgatttatca tgggcgcagg aatctaataa cgtaaatgcg
721 ataatgcagt gctggtatcc aggcgcaaga ggtggacgtg ctattgcaga ggttttattt
781 ggcaaggcca gtccaggcgg taaaatgcct cttacatttt atgcctcaga tgatgacctt
841 cctgattttt ctgattattc aatggaaaat aggacataca gatatttcaa gggcacacca
901 ctttatccat ttggttatgg actaggttat tctaaaattg attatctatt tgcttctatt
961 gataaagata agggagcaat tggtgataca ttcaagctaa aggtagatgt taaaaatacc
1021 ggtaagtata cacagcatga ggctgttcaa gtatatgtaa cggaccttga ggcaacgaca
1081 agagtgccta ttagaagcct tagaaaggtt aaatgtctag agcttgagcc tggtgaaaca
1141 aaagaggttg aatttaccct ttttgcaaga gattttgcca ttattgatga aaggggaaaa
1201 tgtatcatag agccaggcaa gtttaagatt tctattgggg gacaacagcc agacgataga
1261 agtaaagaac ttatgggcag agagtgtgat atttttgaaa ttgaattaac aggctctgtt
1321 acagaagttg aatattaatt gagaggtgca tcatggttat agctaacaat ccaattttaa
1381 aaggttttta tccagaccct tctatctgca gaaaagggga tgatttttat ctagtttgtt
1441 caagttttgt gtatgctccg ggagtaccga tttttcacac taaggatttg gcacattttg
1501 agcaaattgg aaatatatta gacagagaaa gtcaacttcc attgtcggga gatatatcta
1561 gaggcatatt tgccccaaca ataagagagc ataatggaat cttttacatg ataacaacta
1621 atgtaagctc tggcggcaac tttattgtta ctgcaaaaga tccagctggt ccttggtcag
1681 agccatatta tttaggtgaa gatgaggcgc caggtattga tccatctctg ttttttgatg
1741 acgatggcaa atgttattac gttggtacca gaccaaatcc tgatggagtt cgttacaacg
1801 gtgattggga gatatgggtt caagagctgg atttagagca aatgaaactt gtaggtcctt
1861 cgatggcaat ttggaagggc gctcttaagg atgttatttg gccagaagga ccacaccttt
1921 ataagaaaga tggatattat tatcttttac atgcagaagc tggcacaagc tttgaacatg
1981 ctatttctgt agctcgctca aaggagctat tcaaatggtt tgagggatgt cctagaaatc
2041 ctatatttac ccatagaaat ttaggcaagg attatccagt atgcaatgtt ggacatgctg
2101 atttagttga tgatatcaat ggcaactggt atatggtgat gctggcatct agaccatgca
2161 agggaaagtg cagcttggga cgagagacat tccttgcaaa agtaatttgg gaagacggat
2221 ggccagtggt taatccggga gttggtcgtt tgactgatga ggtggagatg gaccttcctg
2281 aatatagatt ctcaaaagag attactacaa aggataaaat gacctttgaa gagacagtcc
2341 tagatgatag atttgttgga attgaaagaa gaagtgagga cttttattcc cttactgaca
2401 atcctggatt cttaagatta aagcttcgtc ctgaggccat agaaaatact ggcaatccat
2461 cttacttagg aattcgtcaa aagactcatt cgtttagagc aagctgtggc cttaagttta
2521 caccagcaaa agataatgaa tgtgcaggaa tggtgttatt ccagaataat gaaaatcact
2581 tggagctttt agttgtaaag aagaaagata agctacagtt taaagtagga ccagttatta
2641 aaggaaccaa aatcagactt gctacttttg atatttcatc aggtgattta gaaattattc
2701 ttgaggcagc aaatcagctg gctaatatct atattaaaaa gaataatgaa aagattcttg
2761 tggcagaatg tattgatttg agcccataca ctaccgaaga atcaggcgga ttcgtaggat
2821 gtaccattgg actatatgct tcttcaaatg gaaagaccag tgataactat tgcgattatt
2881 cctactttac agtagaagaa gtatagcatt ttcaatgagc gaatttgcaa gttttatata
2941 cgggattaat tgtacgtaaa aaccatacag gtgtaaaata gtttccagag aaagtttttt
3001 ctctggaatt ttttattatg gaggggatta tgcttcagga aagtattaag aagttggtac
3061 agtacggtat tgatatgggg cttacaccag aatgtgagag aatatatact acaaatcttt
3121 tgcttgaatg tatgaaagaa gatgagtaca tagatccaga ctgtgattta agcaatatta
3181 tacttgaaga tgtattaaag gaacttttag atgaggcagt taatagaggt atcatagagg
3241 attcagttac acatagggat ttgtttgata caaagctaat gaatcagcta tgcccacgtc
3301 ctaaacaggt tatagatgat tttaaccgta tatacgataa ccatggtcca atagctgcaa
3361 cagattattt ttacaagtta agcaaagcct ctgactatat ccgtacttac agggtaaaaa
3421 aggacctaaa atggacatgc gatacagagt atggcactct tgacataaca attaatctct
3481 ctaagccaga aaaagaccca aaggcaattg ctgcagctaa gaatgcaaaa caatccacat
3541 atccgaagtg ccaattatgt atggaaaatg aaggctatgc tggtcgcatt aatcatcctg
3601 ctagagagaa tcatcgcata attcctataa ctataaataa cagcaactgg ggatttcaat
3661 atagcccata cgtttattac aatgagcatt gcatagtctt taacggagag catactccta
3721 tgaaaataga gcgagctact tttgttaagc tatttgattt tatcaaacta tttccacact
3781 attttttagg aagcaatgct gatttaccaa ttgttggagg atctatttta agccatgacc
3841 atttccaagg cggccattac acatttgcca tggaaaaagc tccaattatt caggaattta
3901 ctgtaaaagg atatgaggat gttaaggctg gtatagttaa atggccactt tcagtaatta
3961 gacttcagtg caaggatgag actagactta ttgatttagc gactaatata ttagacaaat
4021 ggagaaatta caccgatgaa gaggcatata tttttgctga aacagatggt gagcctcaca
4081 atacgattac acctattgct agaaaaagag gggattactt tgaactagat cctctagagt
4141 cgacctgcag gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactggg
//