GenBank-Updates@genbank.bio.net (11/21/90)
LOCUS VCHIRGB 1100 bp ds-DNA BCT 21-NOV-1990
DEFINITION Vibrio cholerae iron-regulated virulence protein (irgB) gene,
complete cds.
ACCESSION M55988
KEYWORDS iron-regulated virulence protein.
SOURCE Vibrio cholerae (strain 0395) DNA.
ORGANISM Vibrio cholerae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 1100)
AUTHORS Goldberg,M.B., Boyko,S.A. and Calderwood,S.B.
TITLE Positive transcriptional regulation of an iron-regulated virulence
gene in Vibrio cholerae
JOURNAL Unpublished (1990)
STANDARD full staff_entry
FEATURES from to/span description
pept 169 1065 iron-regulated virulence protein (irgB)
pept 24 < 1 (c) iron-regulated virulence protein (irgA)
mRNA 60 < 1 (c) iron-regulated virulence protein (irgA)
mRNA 55 1063 iron-regulated virulence protein (irgB)
signal 1063 1092 transcription terminator
signal 18 23 -35_signal
signal 42 47 -10_signal
binding 42 60 DNA homologous to E.coli Fur binding site
binding 154 159 ribosome binding site
BASE COUNT 283 a 272 c 259 g 286 t
ORIGIN
1 gggggatgga ttgaatctgg acatttacca ctcctttaaa tgataattat tcttaatttc
61 agaagcggat tattcatata ttctgtactg gagatagatc gacacatgga atagttcggt
121 ccaaatatgg aacgtctgtg gtaattattc tttaagggtc aaatacctat gcaagatctc
181 agcgccgtaa aagctttcca tgcgctttgc caacataaga gcctgactgc cgctgccaaa
241 gcgcttgaac agcctaaatc caccctgagt cgccgtttgg cgcaacttga agaggacttg
301 ggacaaagct tgttgatgcg tcaaggcaac cgcttaacgc tcaccaaagc aggagaagtg
361 tttgcggttt actcggagca actacttgaa ctggccaata aaagtcagga agcgttgcaa
421 gaattgaaca atcaagtgac tggcgaactc acgctggtgg tacaccccaa tttgatccgc
481 ggctggctca gccaagtatt ggatgagttt atgcagcagc attcgacatt gaagatccgt
541 ctactcagcc agtttcaaca cagtgatgag gtgtttgagc ccgatttgat catttggatt
601 gaacacgccg cccccatggg ttatcgcaaa gaacgcttag gctattggcg ctatgccact
661 tacgcctctc ccaaatattt ggcccatcga gataaaccga cccatcctcg tgagctgatt
721 catcacccat ggattgattt tattgcttgt cggcgcgcgg agcttgaact gcaccatcca
781 gagttcggca gttattcact gccagcacta gagagtcgtt tacaaagcga taatcttgcc
841 atgcaagccg atgcgattgc taaaggtcgt ggtattggtt tgcttcccac ttggtttgcc
901 aatggttttg aaacggcgca tccgggcagc ctcattcctt gcgtcaacgg atggcaatca
961 cagccaacag aaatcaactg cttctatccg ctcggtcgtc acccacttcg gctgcgccta
1021 tttattgatg cgctccgcca agcaaggccc gatgagtggc aataaaaaat cccgccgcag
1081 cgggattttt tatcatcgat
//GenBank-Updates@genbank.bio.net (01/31/91)
LOCUS VCHIRGB 1100 bp ds-DNA BCT 31-JAN-1991
DEFINITION Vibrio cholerae iron-regulated virulence protein (irgB) gene,
complete cds.
ACCESSION M55988
KEYWORDS iron-regulated virulence protein.
SOURCE Vibrio cholerae (strain 0395) DNA.
ORGANISM Vibrio cholerae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 1100)
AUTHORS Goldberg,M.B., Boyko,S.A. and Calderwood,S.B.
TITLE Positive transcriptional regulation of an iron-regulated virulence
gene in Vibrio cholerae
JOURNAL Unpublished (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS complement(<1..24)
/product="iron-regulated virulence protein"
/gene="irgA"
/codon_start=24
mRNA complement(<1..(60.61))
/product="iron-regulated virulence protein"
/gene="irgA"
-35_signal 18..23
/gene="irgB"
-10_signal 42..47
/gene="irgB"
protein_bind 42..60
/note="homologous to E.coli Fur binding site"
mRNA (54.55)..1063
/product="iron-regulated virulence protein"
/gene="irgB"
RBS 154..159
/gene="irgB"
CDS 169..1065
/product="iron-regulated virulence protein"
/gene="irgB"
/codon_start=169
terminator 1063..1092
/gene="irgB"
BASE COUNT 283 a 272 c 259 g 286 t
ORIGIN
1 gggggatgga ttgaatctgg acatttacca ctcctttaaa tgataattat tcttaatttc
61 agaagcggat tattcatata ttctgtactg gagatagatc gacacatgga atagttcggt
121 ccaaatatgg aacgtctgtg gtaattattc tttaagggtc aaatacctat gcaagatctc
181 agcgccgtaa aagctttcca tgcgctttgc caacataaga gcctgactgc cgctgccaaa
241 gcgcttgaac agcctaaatc caccctgagt cgccgtttgg cgcaacttga agaggacttg
301 ggacaaagct tgttgatgcg tcaaggcaac cgcttaacgc tcaccaaagc aggagaagtg
361 tttgcggttt actcggagca actacttgaa ctggccaata aaagtcagga agcgttgcaa
421 gaattgaaca atcaagtgac tggcgaactc acgctggtgg tacaccccaa tttgatccgc
481 ggctggctca gccaagtatt ggatgagttt atgcagcagc attcgacatt gaagatccgt
541 ctactcagcc agtttcaaca cagtgatgag gtgtttgagc ccgatttgat catttggatt
601 gaacacgccg cccccatggg ttatcgcaaa gaacgcttag gctattggcg ctatgccact
661 tacgcctctc ccaaatattt ggcccatcga gataaaccga cccatcctcg tgagctgatt
721 catcacccat ggattgattt tattgcttgt cggcgcgcgg agcttgaact gcaccatcca
781 gagttcggca gttattcact gccagcacta gagagtcgtt tacaaagcga taatcttgcc
841 atgcaagccg atgcgattgc taaaggtcgt ggtattggtt tgcttcccac ttggtttgcc
901 aatggttttg aaacggcgca tccgggcagc ctcattcctt gcgtcaacgg atggcaatca
961 cagccaacag aaatcaactg cttctatccg ctcggtcgtc acccacttcg gctgcgccta
1021 tttattgatg cgctccgcca agcaaggccc gatgagtggc aataaaaaat cccgccgcag
1081 cgggattttt tatcatcgat
//GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS VIBIRGB 1100 bp ds-DNA BCT 10-MAY-1991
DEFINITION Vibrio cholerae iron-regulated virulence protein (irgB) gene,
complete cds.
ACCESSION M55988
KEYWORDS iron-regulated virulence protein.
SOURCE Vibrio cholerae (strain 0395) DNA.
ORGANISM Vibrio cholerae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 1100)
AUTHORS Goldberg,M.B., Boyko,S.A. and Calderwood,S.B.
TITLE Positive transcriptional regulation of an iron-regulated virulence
gene in Vibrio cholerae
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 88, 1125-1129 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
mRNA complement(<1..(60.61))
/product="iron-regulated virulence protein"
/gene="irgA"
CDS complement(<1..24)
/product="iron-regulated virulence protein"
/gene="irgA"
/codon_start=24
-35_signal 18..23
/gene="irgB"
-10_signal 42..47
/gene="irgB"
protein_bind 42..60
/note="homologous to E.coli Fur binding site"
mRNA (54.55)..1063
/product="iron-regulated virulence protein"
/gene="irgB"
RBS 154..159
/gene="irgB"
CDS 169..1065
/product="iron-regulated virulence protein"
/gene="irgB"
/codon_start=169
terminator 1063..1092
/gene="irgB"
BASE COUNT 283 a 272 c 259 g 286 t
ORIGIN
1 gggggatgga ttgaatctgg acatttacca ctcctttaaa tgataattat tcttaatttc
61 agaagcggat tattcatata ttctgtactg gagatagatc gacacatgga atagttcggt
121 ccaaatatgg aacgtctgtg gtaattattc tttaagggtc aaatacctat gcaagatctc
181 agcgccgtaa aagctttcca tgcgctttgc caacataaga gcctgactgc cgctgccaaa
241 gcgcttgaac agcctaaatc caccctgagt cgccgtttgg cgcaacttga agaggacttg
301 ggacaaagct tgttgatgcg tcaaggcaac cgcttaacgc tcaccaaagc aggagaagtg
361 tttgcggttt actcggagca actacttgaa ctggccaata aaagtcagga agcgttgcaa
421 gaattgaaca atcaagtgac tggcgaactc acgctggtgg tacaccccaa tttgatccgc
481 ggctggctca gccaagtatt ggatgagttt atgcagcagc attcgacatt gaagatccgt
541 ctactcagcc agtttcaaca cagtgatgag gtgtttgagc ccgatttgat catttggatt
601 gaacacgccg cccccatggg ttatcgcaaa gaacgcttag gctattggcg ctatgccact
661 tacgcctctc ccaaatattt ggcccatcga gataaaccga cccatcctcg tgagctgatt
721 catcacccat ggattgattt tattgcttgt cggcgcgcgg agcttgaact gcaccatcca
781 gagttcggca gttattcact gccagcacta gagagtcgtt tacaaagcga taatcttgcc
841 atgcaagccg atgcgattgc taaaggtcgt ggtattggtt tgcttcccac ttggtttgcc
901 aatggttttg aaacggcgca tccgggcagc ctcattcctt gcgtcaacgg atggcaatca
961 cagccaacag aaatcaactg cttctatccg ctcggtcgtc acccacttcg gctgcgccta
1021 tttattgatg cgctccgcca agcaaggccc gatgagtggc aataaaaaat cccgccgcag
1081 cgggattttt tatcatcgat
//