GenBank-Updates@genbank.bio.net (02/12/91)
LOCUS BSUHEMAXC 6634 bp ds-DNA BCT 12-FEB-1991
DEFINITION Bacillus subtilis hemAXCDBK gene cluster.
ACCESSION M57676
KEYWORDS NAD(P)H:glutamyl-tRNA reductase; aminolevulinic acid dehydratase;
glutamate-1-semialdehyde 2,1-aminotransferase;
porphobilinogen deaminase; uroporphyrinogen III synthase.
SOURCE B.subtilis DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 6634)
AUTHORS Hansson,M., Rutberg,L., Schroder,I. and Hederstedt,L.
TITLE The Bacillus subtilis hemAXCDBK gene cluster encoding enzymes of
the glutamate to uroporphyrinogen III biosynthetic pathway
JOURNAL Unpublished (1990)
STANDARD simple staff_entry
REFERENCE 2 (sites)
AUTHORS Petricek,M., Rutberg,L., Schroeder,I. and Hederstedt,L.
TITLE Cloning and characterization of the hemA region of the Bacillus
subtilis chromosome
JOURNAL J. Bacteriol. 172, 2250-2258 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS 232..1599
/note="The product of this hemA gene is not an
5-aminoleculinic acid synthase"
/gene="hemA"
/codon_start=1
/product="NAD(P)H:glutamyl-tRNA reductase"
CDS 1607..2437
/note="unidentified gene product"
/gene="hemX"
/evidence=EXPERIMENTAL
/codon_start=1
CDS 2470..3414
/EC_number="4.3.1.8"
/function="hydroxymethilbilane synthesis from
porphobilinogen"
/gene="hemC"
/evidence=EXPERIMENTAL
/codon_start=1
/product="porphobilinogen deaminase"
CDS 3404..4192
/EC_number="4.2.1.24"
/gene="hemD"
/evidence=EXPERIMENTAL
/codon_start=1
/product="uroporphyrinogen III synthase"
CDS 4189..5163
/EC_number="4.2.1.24"
/function="porphobilinogen synthesis"
/gene="hemB"
/evidence=EXPERIMENTAL
/codon_start=1
/product="aminolevulinic acid dehydratase"
CDS 5193..6485
/function="5-aminolevulinic acid synthesis"
/EC_number="5.4.3.8"
/gene="hemK"
/evidence=EXPERIMENTAL
/codon_start=1
/product="glutamate-1-semialdehyde
2,1-aminotransferase"
BASE COUNT 1894 a 1273 c 1669 g 1798 t
ORIGIN
1 atgcatatca ccttcttgtt ttttagagct gatgtgtagt aaatttctgc tgtttttggt
61 attgtcaata ggaatgcttc ttttccctga agctttttct aatatagcat aagaatttta
121 aaatctgttc acattttgtg aaagaaacta tgttataatt attataaata atgagttcta
181 tgttagaatg attataaatt aagattgggt gttgggggtg taattagagc gatgcatata
241 cttgttgtgg gagtagatta taaatccgcc cctattgaga tacgtgaaaa agtaagtttt
301 cagccgaatg agctggcaga agcaatggtg cagctgaaag aagagaaaag cattcttgaa
361 aacatcattg tctcaacctg caaccgcact gaaatttatg cggtagtcga ccagcttcat
421 accggccgtt attatataaa aaagttttta gctgattggt ttcaattaag caaagaagag
481 ctgtcaccgt tcttaacgtt ttatgagagc gatgccgctg ttgagcattt attccgtgta
541 gcctgcggac ttgattctat ggtgattggc gaaacgcaga ttctcggaca ggtacgcgac
601 agctttaaaa cagctcagca agaaaaaacg atcgggacta tttttaatga gctgtttaag
661 caggcagtta cagtgggcaa acggactcac gccgaaacag acattggctc aaatgcggtg
721 tcagtaagct atgctgcagt tgaacttgcc aaaaaaatct tcggaaatct ttcaagcaag
781 cacatattga ttctcggtgc gggaaaaatg ggcgagcttg ctgcggaaaa cctgcacgga
841 cagggaatcg gcaaggtcac tgtcattaac cgaacatact tgaaagcgaa ggagcttgca
901 gaccgttttt caggtgaagc gagaagcttg aatcagcttg aaagcgcgct tgcggaggct
961 gatattttaa tcagttcaac cggtgcaagt gaatttgtcg tgtccaaaga gatgatggaa
1021 aacgcgaata agcttcgcaa gggacgtccg ctgtttatgg tcgacattgc cgtgcctaga
1081 gatcttgatc cggcgctgaa tgatcttgaa ggtgtttttc tttatgatat cgacgatctg
1141 gaaggcattg tagaagcgaa catgaaagag cggagagaaa cagctgaaaa agttgaactg
1201 ttaattgaag aaaccattgt ggaatttaaa caatggatga atacacttgg tgttgtgcct
1261 gttatttctg cattgcgcga aaaggcgctt gccatccagt cagaaacgat ggacagcatt
1321 gagcgtaagc tgcctcactt aagcacaaga gagaaaaaac tgttgaacaa acacaccaaa
1381 agtattatta accaaatgct tcgtgatccg attttaaagg tgaaagagct tgcggcagat
1441 gctgattctg aagaaaagct cgcgttgttt atgcagattt ttgatattga agaagctgcg
1501 ggccgtcaaa tgatgaaaac cgttgaaagc agccagaagg tccactcttt taagaaggct
1561 gaatcaaaag cgggctttag cccacttgta agtgagtgaa agctgaatga ttgatactgc
1621 aatggcaaga cttaatgagg ggacaatcgt catttacgcg ttaagtgtac tcttttattt
1681 tatagatttt cttcaacaca accggaaggc tggaaaaatg gccttctggt tgctttctat
1741 tgtctggact ctgcaaaccg tgtatttggc ctattttatg tgggtgacgg ggcggtttcc
1801 ggtattaaat gtgacagagg cactttattt ttatgcctgg gtgcttgtca cgctgtcact
1861 tgtactgaca aagcttttac gtgttgactt tatcgtgttt tttacaaatg ttataggatt
1921 ttctatgatc gccattcaca cattttcacc gacagagcag cagtcagctg ctttttccgg
1981 gcagcttgta tccgagcttt tggtgattca tattacaatg gcgattcttt catacggcgc
2041 tttttccctt tctttcgttt tttctgtgct atatatgttt caatatcatg tgctgaaaaa
2101 gaaaaagtgg ggaaaatggc tgttgagaat agaagattta tctaagcttg attatatggc
2161 gtatgtttta aatgtcattg gggttccgat gctgctgctg agtttgattc tcggcgtcat
2221 ttgggcgtat gtctcactag aaacgctgta ttggtttgac gccaaagtgc ttggttcgtt
2281 tgtcgtcctg ctgctgtaca gctattatct ttatatcagg ctgattaagg agctgcaagg
2341 aaaggtcgct gcactgtgga atacggcttg ttttctggtg ctgatgatca attatttcct
2401 gcttggaagc ctgtcgcaat tccattggtt cagttaaacg atgtcccaag cagattcggg
2461 aggaaagaaa tgatgagaac gattaaagta ggttccagac ggagcaaact cgctatgact
2521 caaacaaaat gggttattca aaaactgaag gaaatcaatc cttcgtttgc ttttgaaatt
2581 aaagagatcg tgacaaaggg cgaccggatt gtcgatgtta cactctcaaa agtgggtgga
2641 aaagggcttt ttgtcaaaga aattgaacag gcgcttttaa acgaagagat tgatatggca
2701 gtgcacagca tgaaggacat gcctgctgtt ttgcctgaag gccttgtgat cggctgtatt
2761 cctgaacggg aggacccgcg tgatgccctt atttcaaaga atcgcgtaaa gctttcagaa
2821 atgaagaaag gtgctgtcat tggcacaagc agtttaagaa gaagcgcgca gcttttgatt
2881 gagcgccctg accttacaat taaatggatt agaggtaata ttgatacaag acttcaaaag
2941 ctggaaacag aggattatga cgcaattatt ttagcggctg ccggcctttc cagaatgggt
3001 tggaagcaag atgtcgtaac cgaattcctt gagcctgagc gctgtttgcc tgctgtgggg
3061 cagggagccc tggcgattga gtgccgagaa tcggatgaag agctgttggc gttgttttct
3121 cagtttacag atgaatatac aaaacggact gtcttagcgg aacgtgcttt tttaaacgcg
3181 atggagggcg gctgccaggt tccgatcgcg ggctactccg tgttaaatgg acaggatgaa
3241 attgaaatga caggtcttgt cgcttcacct gacggcaaaa tcatttttaa agaaaccgtc
3301 accggaaacg atccggagga agtaggaaag cgctgtgccg ctcttatggc tgacaaagga
3361 gcaaaagatt taattgatcg tgtaaaacgg gagcttgacg aggatggaaa atgattttcc
3421 gttgaaagga aaaacagtgc ttgtcacccg gaataaggca caggcagcat catttcagca
3481 aaaagtggag gcgcttggcg gtaaagcggt tttaacctct ttgattacgt ttcgccgcgc
3541 tttgccgaat gatgttgcgg aacaggtaag agaggatctt gccgcgccag gctggcttgt
3601 ttttacaagt gtgaacgggg cagacttctt tttttcttat ctgaaggaaa atcagcttat
3661 tctccctgcg cataaaaaaa ttgcagccgt cggtgaaaaa accgcgcgcc gtttaaaaat
3721 gcataacgta tcggttgatg tgatgccaca ggagtatatt gctgaacaat tggctgacgc
3781 tcttaagcag catgctgaac cgggggagac cattaccgtg atgaaaggga atttgtcacg
3841 tgatgtgata aaacaagagc ttgtcccgct cggttttgaa gtaaaggaat gggttctcta
3901 cgaaacgatt ccggatgaag aaggcattga ggcattgaaa gacgctgcgg gtcaatattc
3961 ctttgactat gtaacattta cgagttcatc aaccgtacat acgtttatgc atgtcttggg
4021 agaagaatta aaaaagtgga aggcgaatgg gacggcctgt atcagcattg ggcctttaac
4081 aaatgatgcc cttctgacgt acggcatcac atcgcatacg cctgatacat ttacaataga
4141 tggcatgctt gagttaatgt gcagcatgtc aagagaggaa gagagaatat gagtcaatca
4201 tttaatagac accgccgcct gcggacatca aaagcaatga gagaaatggt aaaggaaaca
4261 cgtttgcatc catcagattt tatatatccg atttttgtcg ttgaagggct ggaaggcaaa
4321 aaagctgttc cgtcaatgcc tgatgttcac catgtatcat tagatttatt gaaggatgaa
4381 gtagcggagc tggtcaaact gggcattcaa tctgttatcg tgttcggcat cccggaagaa
4441 aaagatgatt gcggaacaca agcgtaccat gatcacggaa ttgtccaaaa agccatcaca
4501 gaaattaaag aacacttccc tgaaatggtt gttgtcgctg acacgtgcct gtgcgaatat
4561 acagaccacg gccattgcgg acttgtcaaa gacggagtca ttctcaatga tgaatcgctg
4621 gagcttttgg cgcagacagc tgtcagccaa gcgaaagcag gtgcggatat cattgcgcca
4681 tcaaacatga tggacggatt tgttacagtg atcagagaag cacttgataa agaaggattc
4741 gtcaatattc ccatcatgtc ttacgctgtt aaatattcaa gtgagtttta cggtccgttc
4801 cgtgatgcag caaacagcac accgcaattc ggagaccgca aaacatatca gatggaccct
4861 gccaaccgta tggaggcact ccgcgaagca caatcagatg ttgaggaagg cgcggacttt
4921 ttgattgtca aaccttcgct ttcttatatg gatatcatgc gtgacgtaaa aaatgagttt
4981 actttgccgc tcgtcgctta taatgtaagc ggagagtatt caatggtgaa ggctgcagcg
5041 cagaacggct ggatcaaaga aaaagaaatt gtgttggaaa ttttgacaag catgaagcgc
5101 gcgggtgccg acctgattat tacgtatcat gcgaaagacg cagcgaaatg gcttgcggag
5161 taattttatt cagttgacag gagttgaggc agatgagaag ctatgaaaaa tcaaaaacgg
5221 cttttaaaga agcgcaaaaa ctcatgccgg gcggtgtgaa cagtcccgtt cgcgcattta
5281 aatcggtaga catggacccg atttttatgg agcgcggaaa aggctcgaaa atctttgata
5341 ttgacgggaa tgaatatatt gactacgtct tgtcatgggg gcctttaatt ttagggcata
5401 caaatgaccg cgtcgtagaa agcctcaaaa aagtggctga atacgggaca agctttggtg
5461 ctccgactga agtagaaaat gaactggcta agctcgtcat tgatcgtgtg ccatctgtag
5521 aaattgtacg aatggtaagc tccggaacag aggctacaat gagtgccctc cgtttggcaa
5581 ggggctatac gggccgcaac aagattttaa aatttgaggg ctgctaccac ggacacggcg
5641 attctctctt gattaaagct ggttcaggtg ttgccactct cggtctgcct gacagcccgg
5701 gggtgcctga aggcattgcg aaaaacacca tcaccgttcc gtacaatgat ttagaaagtg
5761 taaagcttgc tttccagcaa ttcggtgaag acattgcggg agtcattgta gagccagttg
5821 ccggaaatat gggtgttgtt ccgccgcaag aaggtttcct tcagggtctg cgtgatatca
5881 ctgagcagta cggctccctg ctgatttttg atgaagtgat gactggcttc cgggtcgatt
5941 ataactgcgc tcaaggctac ttcggcgtaa cgcctgatct gacttgttta ggaaaagtaa
6001 tcgggggcgg acttcctgtc ggcgcttatg gcggaaaggc agaaatcatg gagcagatcg
6061 ctccaagcgg tccgatctat caagctggta cattgtcagg caacccgctt gcgatgacgg
6121 ctggcttaga gacattgaaa cagctgacac ctgaatccta caagaatttc atcaaaaaag
6181 gcgacagact ggaagaagga atttcaaaaa ccgccggggc tcatggcatt ccgcatacct
6241 ttaaccgtgc aggttcgatg atcggtttct tctttacaaa cgaaccagtc atcaattatg
6301 aaacagcgaa atcatctgat ttgaagctgt tcgcaagcta ttataaaggg atggcaaatg
6361 aaggggtatt ccttccgcca tcacaattcg aaggtctttt cctctcaacg gcccatactg
6421 atgaagatat tgaaaacaca atccaggcag ctgagaaagt atttgctgag atcagccgca
6481 gataagagtg aaaaccggta tcaaggactc cttgtgccgg ttttttcgtg ctctcccact
6541 catattttct ccagttcata catacacctt tagtgacatc taattttaag gattagatga
6601 aaggaggata tgaacttgcc gcaaaatcat cgat
//GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS BSUHEMAXC 6634 bp ds-DNA BCT 22-FEB-1991
DEFINITION Bacillus subtilis hemAXCDBK gene cluster.
ACCESSION M57676
KEYWORDS NAD(P)H:glutamyl-tRNA reductase; aminolevulinic acid dehydratase;
glutamate-1-semialdehyde 2,1-aminotransferase; porphobilinogen
deaminase; uroporphyrinogen III synthase.
SOURCE B.subtilis DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 6634)
AUTHORS Hansson,M., Rutberg,L., Schroder,I. and Hederstedt,L.
TITLE The Bacillus subtilis hemAXCDBK gene cluster encoding enzymes of
the glutamate to uroporphyrinogen III biosynthetic pathway
JOURNAL Unpublished (1990)
STANDARD simple staff_entry
REFERENCE 2 (sites)
AUTHORS Petricek,M., Rutberg,L., Schroeder,I. and Hederstedt,L.
TITLE Cloning and characterization of the hemA region of the Bacillus
subtilis chromosome
JOURNAL J. Bacteriol. 172, 2250-2258 (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS 232..1599
/note="The product of this hemA gene is not an
5-aminoleculinic acid synthase"
/product="NAD(P)H:glutamyl-tRNA reductase"
/gene="hemA"
/codon_start=232
CDS 1607..2437
/note="unidentified gene product"
/gene="hemX"
/evidence=EXPERIMENTAL
/codon_start=1607
CDS 2470..3414
/EC_number="4.3.1.8"
/function="hydroxymethilbilane synthesis from
porphobilinogen"
/product="porphobilinogen deaminase"
/gene="hemC"
/evidence=EXPERIMENTAL
/codon_start=2470
CDS 3404..4192
/EC_number="4.2.1.24"
/product="uroporphyrinogen III synthase"
/gene="hemD"
/evidence=EXPERIMENTAL
/codon_start=3404
CDS 4189..5163
/EC_number="4.2.1.24"
/function="porphobilinogen synthesis"
/product="aminolevulinic acid dehydratase"
/gene="hemB"
/evidence=EXPERIMENTAL
/codon_start=4189
CDS 5193..6485
/function="5-aminolevulinic acid synthesis"
/EC_number="5.4.3.8"
/product="glutamate-1-semialdehyde 2,1-aminotransferase"
/gene="hemK"
/evidence=EXPERIMENTAL
/codon_start=5193
BASE COUNT 1894 a 1273 c 1669 g 1798 t
ORIGIN
1 atgcatatca ccttcttgtt ttttagagct gatgtgtagt aaatttctgc tgtttttggt
61 attgtcaata ggaatgcttc ttttccctga agctttttct aatatagcat aagaatttta
121 aaatctgttc acattttgtg aaagaaacta tgttataatt attataaata atgagttcta
181 tgttagaatg attataaatt aagattgggt gttgggggtg taattagagc gatgcatata
241 cttgttgtgg gagtagatta taaatccgcc cctattgaga tacgtgaaaa agtaagtttt
301 cagccgaatg agctggcaga agcaatggtg cagctgaaag aagagaaaag cattcttgaa
361 aacatcattg tctcaacctg caaccgcact gaaatttatg cggtagtcga ccagcttcat
421 accggccgtt attatataaa aaagttttta gctgattggt ttcaattaag caaagaagag
481 ctgtcaccgt tcttaacgtt ttatgagagc gatgccgctg ttgagcattt attccgtgta
541 gcctgcggac ttgattctat ggtgattggc gaaacgcaga ttctcggaca ggtacgcgac
601 agctttaaaa cagctcagca agaaaaaacg atcgggacta tttttaatga gctgtttaag
661 caggcagtta cagtgggcaa acggactcac gccgaaacag acattggctc aaatgcggtg
721 tcagtaagct atgctgcagt tgaacttgcc aaaaaaatct tcggaaatct ttcaagcaag
781 cacatattga ttctcggtgc gggaaaaatg ggcgagcttg ctgcggaaaa cctgcacgga
841 cagggaatcg gcaaggtcac tgtcattaac cgaacatact tgaaagcgaa ggagcttgca
901 gaccgttttt caggtgaagc gagaagcttg aatcagcttg aaagcgcgct tgcggaggct
961 gatattttaa tcagttcaac cggtgcaagt gaatttgtcg tgtccaaaga gatgatggaa
1021 aacgcgaata agcttcgcaa gggacgtccg ctgtttatgg tcgacattgc cgtgcctaga
1081 gatcttgatc cggcgctgaa tgatcttgaa ggtgtttttc tttatgatat cgacgatctg
1141 gaaggcattg tagaagcgaa catgaaagag cggagagaaa cagctgaaaa agttgaactg
1201 ttaattgaag aaaccattgt ggaatttaaa caatggatga atacacttgg tgttgtgcct
1261 gttatttctg cattgcgcga aaaggcgctt gccatccagt cagaaacgat ggacagcatt
1321 gagcgtaagc tgcctcactt aagcacaaga gagaaaaaac tgttgaacaa acacaccaaa
1381 agtattatta accaaatgct tcgtgatccg attttaaagg tgaaagagct tgcggcagat
1441 gctgattctg aagaaaagct cgcgttgttt atgcagattt ttgatattga agaagctgcg
1501 ggccgtcaaa tgatgaaaac cgttgaaagc agccagaagg tccactcttt taagaaggct
1561 gaatcaaaag cgggctttag cccacttgta agtgagtgaa agctgaatga ttgatactgc
1621 aatggcaaga cttaatgagg ggacaatcgt catttacgcg ttaagtgtac tcttttattt
1681 tatagatttt cttcaacaca accggaaggc tggaaaaatg gccttctggt tgctttctat
1741 tgtctggact ctgcaaaccg tgtatttggc ctattttatg tgggtgacgg ggcggtttcc
1801 ggtattaaat gtgacagagg cactttattt ttatgcctgg gtgcttgtca cgctgtcact
1861 tgtactgaca aagcttttac gtgttgactt tatcgtgttt tttacaaatg ttataggatt
1921 ttctatgatc gccattcaca cattttcacc gacagagcag cagtcagctg ctttttccgg
1981 gcagcttgta tccgagcttt tggtgattca tattacaatg gcgattcttt catacggcgc
2041 tttttccctt tctttcgttt tttctgtgct atatatgttt caatatcatg tgctgaaaaa
2101 gaaaaagtgg ggaaaatggc tgttgagaat agaagattta tctaagcttg attatatggc
2161 gtatgtttta aatgtcattg gggttccgat gctgctgctg agtttgattc tcggcgtcat
2221 ttgggcgtat gtctcactag aaacgctgta ttggtttgac gccaaagtgc ttggttcgtt
2281 tgtcgtcctg ctgctgtaca gctattatct ttatatcagg ctgattaagg agctgcaagg
2341 aaaggtcgct gcactgtgga atacggcttg ttttctggtg ctgatgatca attatttcct
2401 gcttggaagc ctgtcgcaat tccattggtt cagttaaacg atgtcccaag cagattcggg
2461 aggaaagaaa tgatgagaac gattaaagta ggttccagac ggagcaaact cgctatgact
2521 caaacaaaat gggttattca aaaactgaag gaaatcaatc cttcgtttgc ttttgaaatt
2581 aaagagatcg tgacaaaggg cgaccggatt gtcgatgtta cactctcaaa agtgggtgga
2641 aaagggcttt ttgtcaaaga aattgaacag gcgcttttaa acgaagagat tgatatggca
2701 gtgcacagca tgaaggacat gcctgctgtt ttgcctgaag gccttgtgat cggctgtatt
2761 cctgaacggg aggacccgcg tgatgccctt atttcaaaga atcgcgtaaa gctttcagaa
2821 atgaagaaag gtgctgtcat tggcacaagc agtttaagaa gaagcgcgca gcttttgatt
2881 gagcgccctg accttacaat taaatggatt agaggtaata ttgatacaag acttcaaaag
2941 ctggaaacag aggattatga cgcaattatt ttagcggctg ccggcctttc cagaatgggt
3001 tggaagcaag atgtcgtaac cgaattcctt gagcctgagc gctgtttgcc tgctgtgggg
3061 cagggagccc tggcgattga gtgccgagaa tcggatgaag agctgttggc gttgttttct
3121 cagtttacag atgaatatac aaaacggact gtcttagcgg aacgtgcttt tttaaacgcg
3181 atggagggcg gctgccaggt tccgatcgcg ggctactccg tgttaaatgg acaggatgaa
3241 attgaaatga caggtcttgt cgcttcacct gacggcaaaa tcatttttaa agaaaccgtc
3301 accggaaacg atccggagga agtaggaaag cgctgtgccg ctcttatggc tgacaaagga
3361 gcaaaagatt taattgatcg tgtaaaacgg gagcttgacg aggatggaaa atgattttcc
3421 gttgaaagga aaaacagtgc ttgtcacccg gaataaggca caggcagcat catttcagca
3481 aaaagtggag gcgcttggcg gtaaagcggt tttaacctct ttgattacgt ttcgccgcgc
3541 tttgccgaat gatgttgcgg aacaggtaag agaggatctt gccgcgccag gctggcttgt
3601 ttttacaagt gtgaacgggg cagacttctt tttttcttat ctgaaggaaa atcagcttat
3661 tctccctgcg cataaaaaaa ttgcagccgt cggtgaaaaa accgcgcgcc gtttaaaaat
3721 gcataacgta tcggttgatg tgatgccaca ggagtatatt gctgaacaat tggctgacgc
3781 tcttaagcag catgctgaac cgggggagac cattaccgtg atgaaaggga atttgtcacg
3841 tgatgtgata aaacaagagc ttgtcccgct cggttttgaa gtaaaggaat gggttctcta
3901 cgaaacgatt ccggatgaag aaggcattga ggcattgaaa gacgctgcgg gtcaatattc
3961 ctttgactat gtaacattta cgagttcatc aaccgtacat acgtttatgc atgtcttggg
4021 agaagaatta aaaaagtgga aggcgaatgg gacggcctgt atcagcattg ggcctttaac
4081 aaatgatgcc cttctgacgt acggcatcac atcgcatacg cctgatacat ttacaataga
4141 tggcatgctt gagttaatgt gcagcatgtc aagagaggaa gagagaatat gagtcaatca
4201 tttaatagac accgccgcct gcggacatca aaagcaatga gagaaatggt aaaggaaaca
4261 cgtttgcatc catcagattt tatatatccg atttttgtcg ttgaagggct ggaaggcaaa
4321 aaagctgttc cgtcaatgcc tgatgttcac catgtatcat tagatttatt gaaggatgaa
4381 gtagcggagc tggtcaaact gggcattcaa tctgttatcg tgttcggcat cccggaagaa
4441 aaagatgatt gcggaacaca agcgtaccat gatcacggaa ttgtccaaaa agccatcaca
4501 gaaattaaag aacacttccc tgaaatggtt gttgtcgctg acacgtgcct gtgcgaatat
4561 acagaccacg gccattgcgg acttgtcaaa gacggagtca ttctcaatga tgaatcgctg
4621 gagcttttgg cgcagacagc tgtcagccaa gcgaaagcag gtgcggatat cattgcgcca
4681 tcaaacatga tggacggatt tgttacagtg atcagagaag cacttgataa agaaggattc
4741 gtcaatattc ccatcatgtc ttacgctgtt aaatattcaa gtgagtttta cggtccgttc
4801 cgtgatgcag caaacagcac accgcaattc ggagaccgca aaacatatca gatggaccct
4861 gccaaccgta tggaggcact ccgcgaagca caatcagatg ttgaggaagg cgcggacttt
4921 ttgattgtca aaccttcgct ttcttatatg gatatcatgc gtgacgtaaa aaatgagttt
4981 actttgccgc tcgtcgctta taatgtaagc ggagagtatt caatggtgaa ggctgcagcg
5041 cagaacggct ggatcaaaga aaaagaaatt gtgttggaaa ttttgacaag catgaagcgc
5101 gcgggtgccg acctgattat tacgtatcat gcgaaagacg cagcgaaatg gcttgcggag
5161 taattttatt cagttgacag gagttgaggc agatgagaag ctatgaaaaa tcaaaaacgg
5221 cttttaaaga agcgcaaaaa ctcatgccgg gcggtgtgaa cagtcccgtt cgcgcattta
5281 aatcggtaga catggacccg atttttatgg agcgcggaaa aggctcgaaa atctttgata
5341 ttgacgggaa tgaatatatt gactacgtct tgtcatgggg gcctttaatt ttagggcata
5401 caaatgaccg cgtcgtagaa agcctcaaaa aagtggctga atacgggaca agctttggtg
5461 ctccgactga agtagaaaat gaactggcta agctcgtcat tgatcgtgtg ccatctgtag
5521 aaattgtacg aatggtaagc tccggaacag aggctacaat gagtgccctc cgtttggcaa
5581 ggggctatac gggccgcaac aagattttaa aatttgaggg ctgctaccac ggacacggcg
5641 attctctctt gattaaagct ggttcaggtg ttgccactct cggtctgcct gacagcccgg
5701 gggtgcctga aggcattgcg aaaaacacca tcaccgttcc gtacaatgat ttagaaagtg
5761 taaagcttgc tttccagcaa ttcggtgaag acattgcggg agtcattgta gagccagttg
5821 ccggaaatat gggtgttgtt ccgccgcaag aaggtttcct tcagggtctg cgtgatatca
5881 ctgagcagta cggctccctg ctgatttttg atgaagtgat gactggcttc cgggtcgatt
5941 ataactgcgc tcaaggctac ttcggcgtaa cgcctgatct gacttgttta ggaaaagtaa
6001 tcgggggcgg acttcctgtc ggcgcttatg gcggaaaggc agaaatcatg gagcagatcg
6061 ctccaagcgg tccgatctat caagctggta cattgtcagg caacccgctt gcgatgacgg
6121 ctggcttaga gacattgaaa cagctgacac ctgaatccta caagaatttc atcaaaaaag
6181 gcgacagact ggaagaagga atttcaaaaa ccgccggggc tcatggcatt ccgcatacct
6241 ttaaccgtgc aggttcgatg atcggtttct tctttacaaa cgaaccagtc atcaattatg
6301 aaacagcgaa atcatctgat ttgaagctgt tcgcaagcta ttataaaggg atggcaaatg
6361 aaggggtatt ccttccgcca tcacaattcg aaggtctttt cctctcaacg gcccatactg
6421 atgaagatat tgaaaacaca atccaggcag ctgagaaagt atttgctgag atcagccgca
6481 gataagagtg aaaaccggta tcaaggactc cttgtgccgg ttttttcgtg ctctcccact
6541 catattttct ccagttcata catacacctt tagtgacatc taattttaag gattagatga
6601 aaggaggata tgaacttgcc gcaaaatcat cgat
//GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS BACHEMAXC 6634 bp ds-DNA BCT 10-MAY-1991
DEFINITION Bacillus subtilis hemAXCDBK gene cluster.
ACCESSION M57676
KEYWORDS NAD(P)H:glutamyl-tRNA reductase; aminolevulinic acid dehydratase;
glutamate-1-semialdehyde 2,1-aminotransferase;
porphobilinogen deaminase; uroporphyrinogen III synthase.
SOURCE B.subtilis DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (sites)
AUTHORS Petricek,M., Rutberg,L., Schroeder,I. and Hederstedt,L.
TITLE Cloning and characterization of the hemA region of the Bacillus
subtilis chromosome
JOURNAL J. Bacteriol. 172, 2250-2258 (1990)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 6634)
AUTHORS Hansson,M., Rutberg,L., Schroeder,I. and Hederstedt,L.
TITLE The Bacillus subtilis hemAXCDBL gene cluster, which encodes enzymes
of the Biosynthetic pathway from glutamate to Uroporphyrinogen III
JOURNAL J. Bacteriol. 173, 2590-2599 (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 232..1599
/note="The product of this hemA gene is not an
5-aminoleculinic acid synthase"
/product="NAD(P)H:glutamyl-tRNA reductase"
/gene="hemA"
/codon_start=232
CDS 1607..2437
/note="unidentified gene product"
/gene="hemX"
/evidence=EXPERIMENTAL
/codon_start=1607
CDS 2470..3414
/function="hydroxymethilbilane synthesis from
porphobilinogen"
/EC_number="4.3.1.8"
/product="porphobilinogen deaminase"
/gene="hemC"
/evidence=EXPERIMENTAL
/codon_start=2470
CDS 3404..4192
/EC_number="4.2.1.24"
/product="uroporphyrinogen III synthase"
/gene="hemD"
/evidence=EXPERIMENTAL
/codon_start=3404
CDS 4189..5163
/function="porphobilinogen synthesis"
/EC_number="4.2.1.24"
/product="aminolevulinic acid dehydratase"
/gene="hemB"
/evidence=EXPERIMENTAL
/codon_start=4189
CDS 5193..6485
/function="5-aminolevulinic acid synthesis"
/EC_number="5.4.3.8"
/product="glutamate-1-semialdehyde 2,1-aminotransferase"
/gene="hemK"
/evidence=EXPERIMENTAL
/codon_start=5193
BASE COUNT 1894 a 1273 c 1669 g 1798 t
ORIGIN
1 atgcatatca ccttcttgtt ttttagagct gatgtgtagt aaatttctgc tgtttttggt
61 attgtcaata ggaatgcttc ttttccctga agctttttct aatatagcat aagaatttta
121 aaatctgttc acattttgtg aaagaaacta tgttataatt attataaata atgagttcta
181 tgttagaatg attataaatt aagattgggt gttgggggtg taattagagc gatgcatata
241 cttgttgtgg gagtagatta taaatccgcc cctattgaga tacgtgaaaa agtaagtttt
301 cagccgaatg agctggcaga agcaatggtg cagctgaaag aagagaaaag cattcttgaa
361 aacatcattg tctcaacctg caaccgcact gaaatttatg cggtagtcga ccagcttcat
421 accggccgtt attatataaa aaagttttta gctgattggt ttcaattaag caaagaagag
481 ctgtcaccgt tcttaacgtt ttatgagagc gatgccgctg ttgagcattt attccgtgta
541 gcctgcggac ttgattctat ggtgattggc gaaacgcaga ttctcggaca ggtacgcgac
601 agctttaaaa cagctcagca agaaaaaacg atcgggacta tttttaatga gctgtttaag
661 caggcagtta cagtgggcaa acggactcac gccgaaacag acattggctc aaatgcggtg
721 tcagtaagct atgctgcagt tgaacttgcc aaaaaaatct tcggaaatct ttcaagcaag
781 cacatattga ttctcggtgc gggaaaaatg ggcgagcttg ctgcggaaaa cctgcacgga
841 cagggaatcg gcaaggtcac tgtcattaac cgaacatact tgaaagcgaa ggagcttgca
901 gaccgttttt caggtgaagc gagaagcttg aatcagcttg aaagcgcgct tgcggaggct
961 gatattttaa tcagttcaac cggtgcaagt gaatttgtcg tgtccaaaga gatgatggaa
1021 aacgcgaata agcttcgcaa gggacgtccg ctgtttatgg tcgacattgc cgtgcctaga
1081 gatcttgatc cggcgctgaa tgatcttgaa ggtgtttttc tttatgatat cgacgatctg
1141 gaaggcattg tagaagcgaa catgaaagag cggagagaaa cagctgaaaa agttgaactg
1201 ttaattgaag aaaccattgt ggaatttaaa caatggatga atacacttgg tgttgtgcct
1261 gttatttctg cattgcgcga aaaggcgctt gccatccagt cagaaacgat ggacagcatt
1321 gagcgtaagc tgcctcactt aagcacaaga gagaaaaaac tgttgaacaa acacaccaaa
1381 agtattatta accaaatgct tcgtgatccg attttaaagg tgaaagagct tgcggcagat
1441 gctgattctg aagaaaagct cgcgttgttt atgcagattt ttgatattga agaagctgcg
1501 ggccgtcaaa tgatgaaaac cgttgaaagc agccagaagg tccactcttt taagaaggct
1561 gaatcaaaag cgggctttag cccacttgta agtgagtgaa agctgaatga ttgatactgc
1621 aatggcaaga cttaatgagg ggacaatcgt catttacgcg ttaagtgtac tcttttattt
1681 tatagatttt cttcaacaca accggaaggc tggaaaaatg gccttctggt tgctttctat
1741 tgtctggact ctgcaaaccg tgtatttggc ctattttatg tgggtgacgg ggcggtttcc
1801 ggtattaaat gtgacagagg cactttattt ttatgcctgg gtgcttgtca cgctgtcact
1861 tgtactgaca aagcttttac gtgttgactt tatcgtgttt tttacaaatg ttataggatt
1921 ttctatgatc gccattcaca cattttcacc gacagagcag cagtcagctg ctttttccgg
1981 gcagcttgta tccgagcttt tggtgattca tattacaatg gcgattcttt catacggcgc
2041 tttttccctt tctttcgttt tttctgtgct atatatgttt caatatcatg tgctgaaaaa
2101 gaaaaagtgg ggaaaatggc tgttgagaat agaagattta tctaagcttg attatatggc
2161 gtatgtttta aatgtcattg gggttccgat gctgctgctg agtttgattc tcggcgtcat
2221 ttgggcgtat gtctcactag aaacgctgta ttggtttgac gccaaagtgc ttggttcgtt
2281 tgtcgtcctg ctgctgtaca gctattatct ttatatcagg ctgattaagg agctgcaagg
2341 aaaggtcgct gcactgtgga atacggcttg ttttctggtg ctgatgatca attatttcct
2401 gcttggaagc ctgtcgcaat tccattggtt cagttaaacg atgtcccaag cagattcggg
2461 aggaaagaaa tgatgagaac gattaaagta ggttccagac ggagcaaact cgctatgact
2521 caaacaaaat gggttattca aaaactgaag gaaatcaatc cttcgtttgc ttttgaaatt
2581 aaagagatcg tgacaaaggg cgaccggatt gtcgatgtta cactctcaaa agtgggtgga
2641 aaagggcttt ttgtcaaaga aattgaacag gcgcttttaa acgaagagat tgatatggca
2701 gtgcacagca tgaaggacat gcctgctgtt ttgcctgaag gccttgtgat cggctgtatt
2761 cctgaacggg aggacccgcg tgatgccctt atttcaaaga atcgcgtaaa gctttcagaa
2821 atgaagaaag gtgctgtcat tggcacaagc agtttaagaa gaagcgcgca gcttttgatt
2881 gagcgccctg accttacaat taaatggatt agaggtaata ttgatacaag acttcaaaag
2941 ctggaaacag aggattatga cgcaattatt ttagcggctg ccggcctttc cagaatgggt
3001 tggaagcaag atgtcgtaac cgaattcctt gagcctgagc gctgtttgcc tgctgtgggg
3061 cagggagccc tggcgattga gtgccgagaa tcggatgaag agctgttggc gttgttttct
3121 cagtttacag atgaatatac aaaacggact gtcttagcgg aacgtgcttt tttaaacgcg
3181 atggagggcg gctgccaggt tccgatcgcg ggctactccg tgttaaatgg acaggatgaa
3241 attgaaatga caggtcttgt cgcttcacct gacggcaaaa tcatttttaa agaaaccgtc
3301 accggaaacg atccggagga agtaggaaag cgctgtgccg ctcttatggc tgacaaagga
3361 gcaaaagatt taattgatcg tgtaaaacgg gagcttgacg aggatggaaa atgattttcc
3421 gttgaaagga aaaacagtgc ttgtcacccg gaataaggca caggcagcat catttcagca
3481 aaaagtggag gcgcttggcg gtaaagcggt tttaacctct ttgattacgt ttcgccgcgc
3541 tttgccgaat gatgttgcgg aacaggtaag agaggatctt gccgcgccag gctggcttgt
3601 ttttacaagt gtgaacgggg cagacttctt tttttcttat ctgaaggaaa atcagcttat
3661 tctccctgcg cataaaaaaa ttgcagccgt cggtgaaaaa accgcgcgcc gtttaaaaat
3721 gcataacgta tcggttgatg tgatgccaca ggagtatatt gctgaacaat tggctgacgc
3781 tcttaagcag catgctgaac cgggggagac cattaccgtg atgaaaggga atttgtcacg
3841 tgatgtgata aaacaagagc ttgtcccgct cggttttgaa gtaaaggaat gggttctcta
3901 cgaaacgatt ccggatgaag aaggcattga ggcattgaaa gacgctgcgg gtcaatattc
3961 ctttgactat gtaacattta cgagttcatc aaccgtacat acgtttatgc atgtcttggg
4021 agaagaatta aaaaagtgga aggcgaatgg gacggcctgt atcagcattg ggcctttaac
4081 aaatgatgcc cttctgacgt acggcatcac atcgcatacg cctgatacat ttacaataga
4141 tggcatgctt gagttaatgt gcagcatgtc aagagaggaa gagagaatat gagtcaatca
4201 tttaatagac accgccgcct gcggacatca aaagcaatga gagaaatggt aaaggaaaca
4261 cgtttgcatc catcagattt tatatatccg atttttgtcg ttgaagggct ggaaggcaaa
4321 aaagctgttc cgtcaatgcc tgatgttcac catgtatcat tagatttatt gaaggatgaa
4381 gtagcggagc tggtcaaact gggcattcaa tctgttatcg tgttcggcat cccggaagaa
4441 aaagatgatt gcggaacaca agcgtaccat gatcacggaa ttgtccaaaa agccatcaca
4501 gaaattaaag aacacttccc tgaaatggtt gttgtcgctg acacgtgcct gtgcgaatat
4561 acagaccacg gccattgcgg acttgtcaaa gacggagtca ttctcaatga tgaatcgctg
4621 gagcttttgg cgcagacagc tgtcagccaa gcgaaagcag gtgcggatat cattgcgcca
4681 tcaaacatga tggacggatt tgttacagtg atcagagaag cacttgataa agaaggattc
4741 gtcaatattc ccatcatgtc ttacgctgtt aaatattcaa gtgagtttta cggtccgttc
4801 cgtgatgcag caaacagcac accgcaattc ggagaccgca aaacatatca gatggaccct
4861 gccaaccgta tggaggcact ccgcgaagca caatcagatg ttgaggaagg cgcggacttt
4921 ttgattgtca aaccttcgct ttcttatatg gatatcatgc gtgacgtaaa aaatgagttt
4981 actttgccgc tcgtcgctta taatgtaagc ggagagtatt caatggtgaa ggctgcagcg
5041 cagaacggct ggatcaaaga aaaagaaatt gtgttggaaa ttttgacaag catgaagcgc
5101 gcgggtgccg acctgattat tacgtatcat gcgaaagacg cagcgaaatg gcttgcggag
5161 taattttatt cagttgacag gagttgaggc agatgagaag ctatgaaaaa tcaaaaacgg
5221 cttttaaaga agcgcaaaaa ctcatgccgg gcggtgtgaa cagtcccgtt cgcgcattta
5281 aatcggtaga catggacccg atttttatgg agcgcggaaa aggctcgaaa atctttgata
5341 ttgacgggaa tgaatatatt gactacgtct tgtcatgggg gcctttaatt ttagggcata
5401 caaatgaccg cgtcgtagaa agcctcaaaa aagtggctga atacgggaca agctttggtg
5461 ctccgactga agtagaaaat gaactggcta agctcgtcat tgatcgtgtg ccatctgtag
5521 aaattgtacg aatggtaagc tccggaacag aggctacaat gagtgccctc cgtttggcaa
5581 ggggctatac gggccgcaac aagattttaa aatttgaggg ctgctaccac ggacacggcg
5641 attctctctt gattaaagct ggttcaggtg ttgccactct cggtctgcct gacagcccgg
5701 gggtgcctga aggcattgcg aaaaacacca tcaccgttcc gtacaatgat ttagaaagtg
5761 taaagcttgc tttccagcaa ttcggtgaag acattgcggg agtcattgta gagccagttg
5821 ccggaaatat gggtgttgtt ccgccgcaag aaggtttcct tcagggtctg cgtgatatca
5881 ctgagcagta cggctccctg ctgatttttg atgaagtgat gactggcttc cgggtcgatt
5941 ataactgcgc tcaaggctac ttcggcgtaa cgcctgatct gacttgttta ggaaaagtaa
6001 tcgggggcgg acttcctgtc ggcgcttatg gcggaaaggc agaaatcatg gagcagatcg
6061 ctccaagcgg tccgatctat caagctggta cattgtcagg caacccgctt gcgatgacgg
6121 ctggcttaga gacattgaaa cagctgacac ctgaatccta caagaatttc atcaaaaaag
6181 gcgacagact ggaagaagga atttcaaaaa ccgccggggc tcatggcatt ccgcatacct
6241 ttaaccgtgc aggttcgatg atcggtttct tctttacaaa cgaaccagtc atcaattatg
6301 aaacagcgaa atcatctgat ttgaagctgt tcgcaagcta ttataaaggg atggcaaatg
6361 aaggggtatt ccttccgcca tcacaattcg aaggtctttt cctctcaacg gcccatactg
6421 atgaagatat tgaaaacaca atccaggcag ctgagaaagt atttgctgag atcagccgca
6481 gataagagtg aaaaccggta tcaaggactc cttgtgccgg ttttttcgtg ctctcccact
6541 catattttct ccagttcata catacacctt tagtgacatc taattttaag gattagatga
6601 aaggaggata tgaacttgcc gcaaaatcat cgat
//