GenBank-Updates@genbank.bio.net (04/05/91)
LOCUS MUSGSA 2925 bp ds-DNA ROD 05-APR-1991
DEFINITION Mouse intronless glutamine synthetase gene, complete cds.
ACCESSION M60803
KEYWORDS glutamine synthetase.
SOURCE Mouse DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 2925)
AUTHORS Bhandari,B., Roesler,W.J., DeLisio,K.D., Klemm,D.J., Ross,N. and
Miller,R.E.
TITLE A functional promoter flanks an intronless glutamine synthetase
gene
JOURNAL J. Biol. Chem. (1991) In press
STANDARD simple staff_entry
COMMENT The 5' flanking sequence reported in this paper was also reported
in PNASU 85, 5789-7893, 1988; accession number J03820.
FEATURES Location/Qualifiers
CAAT_signal 918..924
/product="glutamine synthetase"
TATA_signal 1001..1007
/product="glutamine synthetase"
mRNA 1030..>2925
/note="intronless"
/product="glutamine synthetase"
CDS 1252..2367
/EC_number="6.3.1.2"
/note="intronless"
/product="glutamine synthetase"
/codon_start=1252
polyA_signal 2825..2830
/product="glutamine synthetase"
BASE COUNT 829 a 683 c 637 g 776 t
ORIGIN
1 ctaccccaat gtgcaataac acaccttgtt ccagtctaac tacagccctg tcacccttgc
61 acaactttta tccattcctt ctcacccctt cttctttaca gcctctagca actatcattc
121 cactgtaaag gtctttcaga acacatatca gtaagactgc tctgtacctg tcaccagacg
181 cctggctgat ttcccctcca gtttcattca cagtcagacc ttaatacaca cctttagtcc
241 aaataatgaa ggtaagttaa ttggtagaag gtagcagcca tgtttgaaag agaagcaact
301 taagggagca gggcagagag aagaaagaag catttttcat gggagagttt tacagagaag
361 tgttgaagag aaaacaagct agactccagt gaagacagac tgcgtcaggg aatgagaaga
421 agccagaaga ttagaacaga ttgccagagt tagtttgagg ccaagcaaag caattcagtc
481 agaggcctag aagagccagt ttgaatcagt cagcttggaa ggatttgagc cagaagaact
541 gagttaacca cccatccaga gctcagaaga acagatattc agcagtgagt ctcagagcct
601 gaaaaacttc taggcctaga aaggctcata cagagactag aagcttctag gactaggctt
661 aggttagtag acaaaggcag taagctttgg agacaacaat tagatccaga gaataaaaag
721 tacatttaca attttgtcct ttttgtgagt gactaccatt gatggtgtgt gtgtgtgtgt
781 gtgtgtgtgt gtgtgtgtgt gtgtatgtgt acacgtgtat atatgtacca ccatttcccc
841 atccattcct cagctgatga acacttttcc cactgcctgc ttactgtact ggaggcatga
901 gaatgtaaac atttctccaa caatttgact ttatttattc gataagatgt tccccttcca
961 aaaagaaacc attttagata gaaagataca aattataatg taatatattc ataactctat
1021 ctacataata aatgttagtg ctttattcag atctttatta tacaaataaa atgtcattga
1081 taacactcaa cttctacatt tgctcatatg aaatttttag cagagccgag aatgggagta
1141 gagcagagtg tctgaacagc acacccatct cctctctgtt tttcgcctcg ttctcgtgac
1201 ctgttcaccc atccatcatc cagctggcca ctgttctgaa caccttccac catggctacc
1261 tcagcaagtt cccacgtgaa caaaggcatc aagcaaatgt acatgtccct gccccagggt
1321 gagaaaatcc aagccatgta tatctgggtt gatggtaccg gagaaccact gcgctgcaag
1381 acctgtcgta ccctggactg tgagcccaag tgtgtggaag agttacctga gtggaacttt
1441 gatggctcca gtacctttca gtctgaaggc tccaacagca acatgtatct ccatcctgtt
1501 gccatgttta gagacccctt ccgcaacaag ctggtgctat gtgaagtttt caagtataac
1561 cggaagcctg cagagaccaa cttgaggcac atctgtaaac ggataatgga catggtgagc
1621 aaccagcacc cctggtttgg aatggagcag gaatatactc tcatgggaac agacggccac
1681 ccgtttggtt ggcctttcaa tggcttccct ggaccccaag gcccatatta ctgcggtgtg
1741 ggagcagaca aagcctatgg cagggacatc gtggaggctc actaccgggc ctgcttgtat
1801 gccggagtca agatcacggg gacaaatgcg gaggttatgc ctgcccagtg ggaattccag
1861 ataggaccct gtgaggggat ccagatggga gatcatcttt ggatagcctg ttttatcttg
1921 catcgggtat gcgaagactt tggggtgata gcaacctttg accccaagcc cattccaggg
1981 aactgggatg gtgcaggctg ccataccaac ttcagcacca aggccatgcg ggaggagaat
2041 ggtctgaagt gcattgagga ggccattgac aaactgagca agaggcacca gtaccacatc
2101 cacacctacg atcccaaggg gggcctggac aactcccggc gtctgactgg attccacgaa
2161 acctccaaca tcaacgactt ttctgccagt gttgccaacc gcagtgccag tatccgcatt
2221 ccctggactg tcggccagga gaagaagggc tactttgaag accgtcggcc ttctgccaat
2281 tgtgacccct atgcggtgac agaagccatc gtccgcacgt gtctcctcaa cgaaacaggc
2341 gacgaaccct tccaatacaa gaactaagca gactagactt ccagtgatcc ctctcccagc
2401 tcttccctct cccagttgtc cccactgtaa ctcaaaggat ggaataccaa ggtcttttta
2461 ttcttcgtgc caaaaaaaag agaaattttt aacttactgt gaatctaatc atattcattt
2521 ttttccattt ttatattatc catgaacaac ttttagtgat ctttgtctac tacattttat
2581 tatgtttttg attacatatt attctaaaaa cagcaccatt cccttttttt atttcattat
2641 tatgttccat agaggagcag tgtttagact ctgttccaaa acctccagca aaggtataat
2701 tatgggacac cctggcatag gagaatgtga agtttacatg atctgcccct agactttgag
2761 gcaaagaaag caaagatgtg gtggtaccca tagtgagtct cccagactgt aaacctatgg
2821 gcacaataaa tggtatctag ttttaactat tcaggattgg ggtaacttat tatgtagaca
2881 tagtaacttg aggtaccgga cttccttgcc ttggccacta aattc
//GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS MUSGSA 2925 bp ds-DNA ROD 10-MAY-1991
DEFINITION Mouse intronless glutamine synthetase gene, complete cds.
ACCESSION M60803
KEYWORDS glutamine synthetase.
SOURCE Mouse DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 2925)
AUTHORS Bhandari,B., Roesler,W.J., DeLisio,K.D., Klemm,D.J., Ross,N.S. and
,M.R.E..
TITLE A functional promoter flanks in intronless glutamine synthetase
gene
JOURNAL J. Biol. Chem. 266, 7784-7792 (1991)
STANDARD simple staff_entry
COMMENT The 5' flanking sequence reported in this paper was also reported
in PNASU 85, 5789-7893, 1988; accession number J03820.
FEATURES Location/Qualifiers
CAAT_signal 918..924
/product="glutamine synthetase"
TATA_signal 1001..1007
/product="glutamine synthetase"
mRNA 1030..>2925
/note="intronless"
/product="glutamine synthetase"
CDS 1252..2367
/EC_number="6.3.1.2"
/note="intronless"
/product="glutamine synthetase"
/codon_start=1252
polyA_signal 2825..2830
/product="glutamine synthetase"
BASE COUNT 829 a 683 c 637 g 776 t
ORIGIN
1 ctaccccaat gtgcaataac acaccttgtt ccagtctaac tacagccctg tcacccttgc
61 acaactttta tccattcctt ctcacccctt cttctttaca gcctctagca actatcattc
121 cactgtaaag gtctttcaga acacatatca gtaagactgc tctgtacctg tcaccagacg
181 cctggctgat ttcccctcca gtttcattca cagtcagacc ttaatacaca cctttagtcc
241 aaataatgaa ggtaagttaa ttggtagaag gtagcagcca tgtttgaaag agaagcaact
301 taagggagca gggcagagag aagaaagaag catttttcat gggagagttt tacagagaag
361 tgttgaagag aaaacaagct agactccagt gaagacagac tgcgtcaggg aatgagaaga
421 agccagaaga ttagaacaga ttgccagagt tagtttgagg ccaagcaaag caattcagtc
481 agaggcctag aagagccagt ttgaatcagt cagcttggaa ggatttgagc cagaagaact
541 gagttaacca cccatccaga gctcagaaga acagatattc agcagtgagt ctcagagcct
601 gaaaaacttc taggcctaga aaggctcata cagagactag aagcttctag gactaggctt
661 aggttagtag acaaaggcag taagctttgg agacaacaat tagatccaga gaataaaaag
721 tacatttaca attttgtcct ttttgtgagt gactaccatt gatggtgtgt gtgtgtgtgt
781 gtgtgtgtgt gtgtgtgtgt gtgtatgtgt acacgtgtat atatgtacca ccatttcccc
841 atccattcct cagctgatga acacttttcc cactgcctgc ttactgtact ggaggcatga
901 gaatgtaaac atttctccaa caatttgact ttatttattc gataagatgt tccccttcca
961 aaaagaaacc attttagata gaaagataca aattataatg taatatattc ataactctat
1021 ctacataata aatgttagtg ctttattcag atctttatta tacaaataaa atgtcattga
1081 taacactcaa cttctacatt tgctcatatg aaatttttag cagagccgag aatgggagta
1141 gagcagagtg tctgaacagc acacccatct cctctctgtt tttcgcctcg ttctcgtgac
1201 ctgttcaccc atccatcatc cagctggcca ctgttctgaa caccttccac catggctacc
1261 tcagcaagtt cccacgtgaa caaaggcatc aagcaaatgt acatgtccct gccccagggt
1321 gagaaaatcc aagccatgta tatctgggtt gatggtaccg gagaaccact gcgctgcaag
1381 acctgtcgta ccctggactg tgagcccaag tgtgtggaag agttacctga gtggaacttt
1441 gatggctcca gtacctttca gtctgaaggc tccaacagca acatgtatct ccatcctgtt
1501 gccatgttta gagacccctt ccgcaacaag ctggtgctat gtgaagtttt caagtataac
1561 cggaagcctg cagagaccaa cttgaggcac atctgtaaac ggataatgga catggtgagc
1621 aaccagcacc cctggtttgg aatggagcag gaatatactc tcatgggaac agacggccac
1681 ccgtttggtt ggcctttcaa tggcttccct ggaccccaag gcccatatta ctgcggtgtg
1741 ggagcagaca aagcctatgg cagggacatc gtggaggctc actaccgggc ctgcttgtat
1801 gccggagtca agatcacggg gacaaatgcg gaggttatgc ctgcccagtg ggaattccag
1861 ataggaccct gtgaggggat ccagatggga gatcatcttt ggatagcctg ttttatcttg
1921 catcgggtat gcgaagactt tggggtgata gcaacctttg accccaagcc cattccaggg
1981 aactgggatg gtgcaggctg ccataccaac ttcagcacca aggccatgcg ggaggagaat
2041 ggtctgaagt gcattgagga ggccattgac aaactgagca agaggcacca gtaccacatc
2101 cacacctacg atcccaaggg gggcctggac aactcccggc gtctgactgg attccacgaa
2161 acctccaaca tcaacgactt ttctgccagt gttgccaacc gcagtgccag tatccgcatt
2221 ccctggactg tcggccagga gaagaagggc tactttgaag accgtcggcc ttctgccaat
2281 tgtgacccct atgcggtgac agaagccatc gtccgcacgt gtctcctcaa cgaaacaggc
2341 gacgaaccct tccaatacaa gaactaagca gactagactt ccagtgatcc ctctcccagc
2401 tcttccctct cccagttgtc cccactgtaa ctcaaaggat ggaataccaa ggtcttttta
2461 ttcttcgtgc caaaaaaaag agaaattttt aacttactgt gaatctaatc atattcattt
2521 ttttccattt ttatattatc catgaacaac ttttagtgat ctttgtctac tacattttat
2581 tatgtttttg attacatatt attctaaaaa cagcaccatt cccttttttt atttcattat
2641 tatgttccat agaggagcag tgtttagact ctgttccaaa acctccagca aaggtataat
2701 tatgggacac cctggcatag gagaatgtga agtttacatg atctgcccct agactttgag
2761 gcaaagaaag caaagatgtg gtggtaccca tagtgagtct cccagactgt aaacctatgg
2821 gcacaataaa tggtatctag ttttaactat tcaggattgg ggtaacttat tatgtagaca
2881 tagtaacttg aggtaccgga cttccttgcc ttggccacta aattc
//