GenBank-Updates@genbank.bio.net (04/10/91)
LOCUS VIBIRGAA 2173 bp ds-DNA BCT 10-APR-1991
DEFINITION V.cholerae iron-regulated outer membrane virulence protein gene,
complete cds.
ACCESSION M63192
KEYWORDS iron-regulated virulence protein.
SOURCE V.cholerae (strain 0395) DNA.
ORGANISM Vibrio cholerae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 2173)
AUTHORS Goldberg,M.B., Boyko,S.A., Butterton,J.R., Stoebner,J.A.,
Payne,S.M. and Calderwood,S.B.
TITLE Nucleotide sequence of irgA, an iron-regulated virulence gene of
Vibrio cholerae, with homology to the Escherichia coli colicin I
receptor
JOURNAL Unpublished (1991)
STANDARD simple staff_entry
REFERENCE 2 (sites)
AUTHORS Goldberg,M.B., Boyko,S.A. and Calderwood,S.B.
TITLE Transcriptional regulation by iron of a Vibrio cholerae virulence
gene and homology of the gene to the Escherichia coli Fur system
JOURNAL J. Bacteriol. 172, 6863-6870 (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
-10_signal 123..128
/note="no -35 signal was identified for irgA,
transcription of irgA requires a trans-acting positive
activator, irgB (GenBank M55988), which is upstream and
divergent from irgA"
/product="IrgA protein"
/gene="irgA"
/evidence=EXPERIMENTAL
RBS 162..166
/product="IrgA protein"
/gene="irgA"
CDS 173..2131
/function="iron-regulated outer membrane protein"
/product="IrgA protein"
/gene="irgA"
/codon_start=173
terminator 2144..2173
/product="IrgA protein"
/gene="irgA"
/evidence=EXPERIMENTAL
BASE COUNT 592 a 512 c 544 g 525 t
ORIGIN
1 aagcttttac ggcgctgaga tcttgcatag gtatttgacc cttaaagaat aattaccaca
61 gacgttccat atttggaccg aactattcca tgtgtcgatc tatctccagt acagaatata
121 tgaataatcc gcttctgaaa ttaagaataa ttatcattta aaggagtggt aaatgtccag
181 attcaatcca tcccccgtca gtttatctgt gacactaggc ttaatgtttt cggctagcgc
241 ttttgctcaa gacgcgacga aaacggatga aaccatggtg gtcactgcgg cgggatacgc
301 gcaagtgatt caaaatgcac cagccagtat cagtgtgatt tcaagagaag atctggaatc
361 tcgctattac cgtgatgtga ccgatgcgct aaaaagcgta ccgggtgtga cagtcaccgg
421 agggggcgat actaccgata tcagcattcg tggtatggga tcaaactata ctcttatctt
481 ggtggatggt aagcgccaaa cctcacgcca gacccgtcca aacagcgatg gcccgggcat
541 tgagcaaggt tggttaccgc cactgcaagc gattgaacgt atcgaggtga tccgtggccc
601 gatgtctacg ctgtacggct cggatgcgat tggcggcgtg atcaacatca tcactcgtaa
661 agatcagcag cagtggtcag gcaatgtgca gctatcgacc gtggtacaag aaaatcgcgc
721 ctcaggcgat gaacaaagcg ctaatttctt cgtaacagga cctttaagtg atgctctatc
781 gctgcaagtc tacggacaaa ccacgcaacg cgatgaagac gaaattgagc atggttacgg
841 cgataaatca ctgcgcagct taacctcaaa gctcaactac cagcttaatc cagatcatca
901 actgcaactg gaagccggtg tatcagccca agatcgcgaa aataacgtgg gcaagtcggc
961 gcaatccagc ggatgtcgcg gtacatgcag caataccgat aaccagtacc gtcgcaacca
1021 tgttgccgtc tctcaccaag gggattggca gggtgttggg cagtctgata cttatctgca
1081 atatgaagag aacaccaata aatctcgtga gatgagcatt gataatacgg tgttcaaatc
1141 cacgttagtg gctcctattg gtgagcatat gttgagcttt ggtgtggaag gtaaacacga
1201 aagtttagag gataaaacct ccaacaaaat ttcgtctcgc acgcatatct cgaacactca
1261 atgggcggga ttcattgaag atgaatgggc gctcgcagag caattccgcc tgacctttgg
1321 tggccgtctg gatcatgata aaaactacgg cagccacttc agcccacgtg tgtatggcgt
1381 atggaatctc gatcctctat ggacagtaaa aggtggtgtt tctactggct tccgtgcacc
1441 gcaattacgt gaagtgacgc cggattgggg gcaagttagt ggcggcggta atatttacgg
1501 caacccggat ttacagcctg aaacttcgat taacaaagaa ctgagtttga tgtacagcac
1561 tggctcaggt ctggcggcat cgttaaccgc tttccataat gattttaaag ataaaatcac
1621 ccgcgtcgct tgccctgcaa atatttgtac tgccggcccc aaccaatggg gtgcaactcc
1681 tacttatcgt gtgaatattg atgaagcgga aacctatggt gcagaagcta cgttaagtct
1741 gccgattacc gaaagcgttg agttatcgtc tagctacacg tacacccatt cggagcaaaa
1801 atcagggaat tttgctggcc gtccgctact gcagttacct aaacatctgt tcaatgccaa
1861 cttgagctgg caaaccacag atcgcctcaa tagctgggct aacctcaact atcgtggtaa
1921 ggagatgcag ccggagggtg gggcatcgaa cgatgatttc atcgcgccaa gctacacctt
1981 tatcgatact ggcgtgactt acgcactcac cgataccgca acgattaaag ctgcggtgta
2041 caacctgttt gatcaagagg tgaattacgc ggagtatggc tatgtagaag atggccgccg
2101 ctactggttg ggtttagaca tcgccttcta attgctagag gttcagagga ttggcggcag
2161 cgccaatcct ctg
//GenBank-Updates@genbank.bio.net (04/16/91)
LOCUS VIBIRGAA 2173 bp ds-DNA BCT 16-APR-1991
DEFINITION V.cholerae iron-regulated outer membrane virulence protein gene,
complete cds.
ACCESSION M63192
KEYWORDS iron-regulated virulence protein.
SOURCE V.cholerae (strain 0395) DNA.
ORGANISM Vibrio cholerae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 2173)
AUTHORS Goldberg,M.B., Boyko,S.A., Butterton,J.R., Stoebner,J.A.,
Payne,S.M. and Calderwood,S.B.
TITLE Nucleotide sequence of irgA, an iron-regulated virulence gene of
Vibrio cholerae, with homology to the Escherichia coli colicin I
receptor
JOURNAL Unpublished (1991)
STANDARD full staff_entry
REFERENCE 2 (sites)
AUTHORS Goldberg,M.B., Boyko,S.A. and Calderwood,S.B.
TITLE Transcriptional regulation by iron of a Vibrio cholerae virulence
gene and homology of the gene to the Escherichia coli Fur system
JOURNAL J. Bacteriol. 172, 6863-6870 (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
misc_feature 1..6
/note="base number 1 of this sequence corresponds to base
number 905 of teh previously reported sequence of the
amino terminus of IrgA (GenBank M37773)"
-10_signal 123..128
/note="no -35 signal was identified for irgA,
transcription of irgA requires a trans-acting positive
activator, irgB (GenBank M55988), which is upstream and
divergent from irgA"
/product="IrgA protein"
/gene="irgA"
/evidence=EXPERIMENTAL
misc_signal 136..156
/function="Dyad homologous to E.coli Fur binding sites"
/phenotype="potential role in iron regulation"
RBS 162..166
/product="IrgA protein"
/gene="irgA"
CDS 173..2131
/function="iron-regulated outer membrane protein"
/product="IrgA protein"
/gene="irgA"
/codon_start=173
misc_feature 269..289
/function="potential interaction site of IrgA with
TonB-like protein"
/phenotype="TonB Box"
/standard_name="TonB Box"
/note="region of the protein homologous to the TonB box of
TonB-dependent outer membrane proteins of E.coli"
terminator 2144..2173
/product="IrgA protein"
/gene="irgA"
/evidence=EXPERIMENTAL
BASE COUNT 592 a 512 c 544 g 525 t
ORIGIN
1 aagcttttac ggcgctgaga tcttgcatag gtatttgacc cttaaagaat aattaccaca
61 gacgttccat atttggaccg aactattcca tgtgtcgatc tatctccagt acagaatata
121 tgaataatcc gcttctgaaa ttaagaataa ttatcattta aaggagtggt aaatgtccag
181 attcaatcca tcccccgtca gtttatctgt gacactaggc ttaatgtttt cggctagcgc
241 ttttgctcaa gacgcgacga aaacggatga aaccatggtg gtcactgcgg cgggatacgc
301 gcaagtgatt caaaatgcac cagccagtat cagtgtgatt tcaagagaag atctggaatc
361 tcgctattac cgtgatgtga ccgatgcgct aaaaagcgta ccgggtgtga cagtcaccgg
421 agggggcgat actaccgata tcagcattcg tggtatggga tcaaactata ctcttatctt
481 ggtggatggt aagcgccaaa cctcacgcca gacccgtcca aacagcgatg gcccgggcat
541 tgagcaaggt tggttaccgc cactgcaagc gattgaacgt atcgaggtga tccgtggccc
601 gatgtctacg ctgtacggct cggatgcgat tggcggcgtg atcaacatca tcactcgtaa
661 agatcagcag cagtggtcag gcaatgtgca gctatcgacc gtggtacaag aaaatcgcgc
721 ctcaggcgat gaacaaagcg ctaatttctt cgtaacagga cctttaagtg atgctctatc
781 gctgcaagtc tacggacaaa ccacgcaacg cgatgaagac gaaattgagc atggttacgg
841 cgataaatca ctgcgcagct taacctcaaa gctcaactac cagcttaatc cagatcatca
901 actgcaactg gaagccggtg tatcagccca agatcgcgaa aataacgtgg gcaagtcggc
961 gcaatccagc ggatgtcgcg gtacatgcag caataccgat aaccagtacc gtcgcaacca
1021 tgttgccgtc tctcaccaag gggattggca gggtgttggg cagtctgata cttatctgca
1081 atatgaagag aacaccaata aatctcgtga gatgagcatt gataatacgg tgttcaaatc
1141 cacgttagtg gctcctattg gtgagcatat gttgagcttt ggtgtggaag gtaaacacga
1201 aagtttagag gataaaacct ccaacaaaat ttcgtctcgc acgcatatct cgaacactca
1261 atgggcggga ttcattgaag atgaatgggc gctcgcagag caattccgcc tgacctttgg
1321 tggccgtctg gatcatgata aaaactacgg cagccacttc agcccacgtg tgtatggcgt
1381 atggaatctc gatcctctat ggacagtaaa aggtggtgtt tctactggct tccgtgcacc
1441 gcaattacgt gaagtgacgc cggattgggg gcaagttagt ggcggcggta atatttacgg
1501 caacccggat ttacagcctg aaacttcgat taacaaagaa ctgagtttga tgtacagcac
1561 tggctcaggt ctggcggcat cgttaaccgc tttccataat gattttaaag ataaaatcac
1621 ccgcgtcgct tgccctgcaa atatttgtac tgccggcccc aaccaatggg gtgcaactcc
1681 tacttatcgt gtgaatattg atgaagcgga aacctatggt gcagaagcta cgttaagtct
1741 gccgattacc gaaagcgttg agttatcgtc tagctacacg tacacccatt cggagcaaaa
1801 atcagggaat tttgctggcc gtccgctact gcagttacct aaacatctgt tcaatgccaa
1861 cttgagctgg caaaccacag atcgcctcaa tagctgggct aacctcaact atcgtggtaa
1921 ggagatgcag ccggagggtg gggcatcgaa cgatgatttc atcgcgccaa gctacacctt
1981 tatcgatact ggcgtgactt acgcactcac cgataccgca acgattaaag ctgcggtgta
2041 caacctgttt gatcaagagg tgaattacgc ggagtatggc tatgtagaag atggccgccg
2101 ctactggttg ggtttagaca tcgccttcta attgctagag gttcagagga ttggcggcag
2161 cgccaatcct ctg
//GenBank-Updates@genbank.bio.net (04/17/91)
LOCUS VIBIRGAA 2173 bp ds-DNA BCT 17-APR-1991
DEFINITION V.cholerae iron-regulated outer membrane virulence protein gene,
complete cds.
ACCESSION M63192
KEYWORDS iron-regulated virulence protein.
SOURCE V.cholerae (strain 0395) DNA.
ORGANISM Vibrio cholerae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 2173)
AUTHORS Goldberg,M.B., Boyko,S.A., Butterton,J.R., Stoebner,J.A.,
Payne,S.M. and Calderwood,S.B.
TITLE Nucleotide sequence of irgA, an iron-regulated virulence gene of
Vibrio cholerae, with homology to the Escherichia coli colicin I
receptor
JOURNAL Unpublished (1991)
STANDARD full staff_entry
REFERENCE 2 (sites)
AUTHORS Goldberg,M.B., Boyko,S.A. and Calderwood,S.B.
TITLE Transcriptional regulation by iron of a Vibrio cholerae virulence
gene and homology of the gene to the Escherichia coli Fur system
JOURNAL J. Bacteriol. 172, 6863-6870 (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
misc_feature complement(1..6)
/function="HindIII restriction enzyme site"
/note="base number 1 of this sequence corresponds to base
number 905 of teh previously reported sequence of the
amino terminus of IrgA (GenBank M37773)"
-10_signal 123..128
/note="no -35 signal was identified for irgA,
transcription of irgA requires a trans-acting positive
activator, irgB (GenBank M55988), which is upstream and
divergent from irgA"
/product="IrgA protein"
/gene="irgA"
/evidence=EXPERIMENTAL
misc_signal 136..156
/function="Dyad homologous to E.coli Fur binding sites"
/phenotype="potential role in iron regulation"
RBS 162..166
/product="IrgA protein"
/gene="irgA"
CDS 173..2131
/function="iron-regulated outer membrane protein"
/product="IrgA protein"
/gene="irgA"
/codon_start=173
misc_feature 269..289
/function="potential interaction site of IrgA with
TonB-like protein"
/phenotype="TonB Box"
/standard_name="TonB Box"
/note="region of the protein homologous to the TonB box of
TonB-dependent outer membrane proteins of E.coli"
terminator 2144..2173
/product="IrgA protein"
/gene="irgA"
/evidence=EXPERIMENTAL
BASE COUNT 592 a 512 c 544 g 525 t
ORIGIN
1 aagcttttac ggcgctgaga tcttgcatag gtatttgacc cttaaagaat aattaccaca
61 gacgttccat atttggaccg aactattcca tgtgtcgatc tatctccagt acagaatata
121 tgaataatcc gcttctgaaa ttaagaataa ttatcattta aaggagtggt aaatgtccag
181 attcaatcca tcccccgtca gtttatctgt gacactaggc ttaatgtttt cggctagcgc
241 ttttgctcaa gacgcgacga aaacggatga aaccatggtg gtcactgcgg cgggatacgc
301 gcaagtgatt caaaatgcac cagccagtat cagtgtgatt tcaagagaag atctggaatc
361 tcgctattac cgtgatgtga ccgatgcgct aaaaagcgta ccgggtgtga cagtcaccgg
421 agggggcgat actaccgata tcagcattcg tggtatggga tcaaactata ctcttatctt
481 ggtggatggt aagcgccaaa cctcacgcca gacccgtcca aacagcgatg gcccgggcat
541 tgagcaaggt tggttaccgc cactgcaagc gattgaacgt atcgaggtga tccgtggccc
601 gatgtctacg ctgtacggct cggatgcgat tggcggcgtg atcaacatca tcactcgtaa
661 agatcagcag cagtggtcag gcaatgtgca gctatcgacc gtggtacaag aaaatcgcgc
721 ctcaggcgat gaacaaagcg ctaatttctt cgtaacagga cctttaagtg atgctctatc
781 gctgcaagtc tacggacaaa ccacgcaacg cgatgaagac gaaattgagc atggttacgg
841 cgataaatca ctgcgcagct taacctcaaa gctcaactac cagcttaatc cagatcatca
901 actgcaactg gaagccggtg tatcagccca agatcgcgaa aataacgtgg gcaagtcggc
961 gcaatccagc ggatgtcgcg gtacatgcag caataccgat aaccagtacc gtcgcaacca
1021 tgttgccgtc tctcaccaag gggattggca gggtgttggg cagtctgata cttatctgca
1081 atatgaagag aacaccaata aatctcgtga gatgagcatt gataatacgg tgttcaaatc
1141 cacgttagtg gctcctattg gtgagcatat gttgagcttt ggtgtggaag gtaaacacga
1201 aagtttagag gataaaacct ccaacaaaat ttcgtctcgc acgcatatct cgaacactca
1261 atgggcggga ttcattgaag atgaatgggc gctcgcagag caattccgcc tgacctttgg
1321 tggccgtctg gatcatgata aaaactacgg cagccacttc agcccacgtg tgtatggcgt
1381 atggaatctc gatcctctat ggacagtaaa aggtggtgtt tctactggct tccgtgcacc
1441 gcaattacgt gaagtgacgc cggattgggg gcaagttagt ggcggcggta atatttacgg
1501 caacccggat ttacagcctg aaacttcgat taacaaagaa ctgagtttga tgtacagcac
1561 tggctcaggt ctggcggcat cgttaaccgc tttccataat gattttaaag ataaaatcac
1621 ccgcgtcgct tgccctgcaa atatttgtac tgccggcccc aaccaatggg gtgcaactcc
1681 tacttatcgt gtgaatattg atgaagcgga aacctatggt gcagaagcta cgttaagtct
1741 gccgattacc gaaagcgttg agttatcgtc tagctacacg tacacccatt cggagcaaaa
1801 atcagggaat tttgctggcc gtccgctact gcagttacct aaacatctgt tcaatgccaa
1861 cttgagctgg caaaccacag atcgcctcaa tagctgggct aacctcaact atcgtggtaa
1921 ggagatgcag ccggagggtg gggcatcgaa cgatgatttc atcgcgccaa gctacacctt
1981 tatcgatact ggcgtgactt acgcactcac cgataccgca acgattaaag ctgcggtgta
2041 caacctgttt gatcaagagg tgaattacgc ggagtatggc tatgtagaag atggccgccg
2101 ctactggttg ggtttagaca tcgccttcta attgctagag gttcagagga ttggcggcag
2161 cgccaatcct ctg
//GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS VIBIRGAA 2173 bp ds-DNA BCT 10-MAY-1991
DEFINITION V.cholerae iron-regulated outer membrane virulence protein gene,
complete cds.
ACCESSION M63192
KEYWORDS iron-regulated virulence protein.
SOURCE V.cholerae (strain 0395) DNA.
ORGANISM Vibrio cholerae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 2173)
AUTHORS Goldberg,M.B., Boyko,S.A., Butterton,J.R., Stoebner,J.A.,
Payne,S.M. and Calderwood,S.B.
TITLE Nucleotide sequence of irgA, an iron-regulated virulence gene of
Vibrio cholerae, with homology to the Escherichia coli colicin I
receptor
JOURNAL Unpublished (1991)
STANDARD full staff_entry
REFERENCE 2 (sites)
AUTHORS Goldberg,M.B., Boyko,S.A. and Calderwood,S.B.
TITLE Transcriptional regulation by iron of a Vibrio cholerae virulence
gene and homology of the gene to the Escherichia coli Fur system
JOURNAL J. Bacteriol. 172, 6863-6870 (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
misc_feature complement(1..6)
/function="HindIII restriction enzyme site"
/note="base number 1 of this sequence corresponds to base
number 905 of the previously reported sequence of the
amino terminus of IrgA (GenBank M37773)"
-10_signal 123..128
/note="no -35 signal was identified for irgA,
transcription of irgA requires a trans-acting positive
activator, irgB (GenBank M55988), which is upstream and
divergent from irgA"
/product="IrgA protein"
/gene="irgA"
/evidence=EXPERIMENTAL
misc_signal 136..156
/function="Dyad homologous to E.coli Fur binding sites"
/phenotype="potential role in iron regulation"
RBS 162..166
/product="IrgA protein"
/gene="irgA"
CDS 173..2131
/function="iron-regulated outer membrane protein"
/product="IrgA protein"
/gene="irgA"
/codon_start=173
misc_feature 269..289
/function="potential interaction site of IrgA with
TonB-like protein"
/phenotype="TonB Box"
/standard_name="TonB Box"
/note="region of the protein homologous to the TonB box of
TonB-dependent outer membrane proteins of E.coli"
terminator 2144..2173
/product="IrgA protein"
/gene="irgA"
/evidence=EXPERIMENTAL
BASE COUNT 592 a 512 c 544 g 525 t
ORIGIN
1 aagcttttac ggcgctgaga tcttgcatag gtatttgacc cttaaagaat aattaccaca
61 gacgttccat atttggaccg aactattcca tgtgtcgatc tatctccagt acagaatata
121 tgaataatcc gcttctgaaa ttaagaataa ttatcattta aaggagtggt aaatgtccag
181 attcaatcca tcccccgtca gtttatctgt gacactaggc ttaatgtttt cggctagcgc
241 ttttgctcaa gacgcgacga aaacggatga aaccatggtg gtcactgcgg cgggatacgc
301 gcaagtgatt caaaatgcac cagccagtat cagtgtgatt tcaagagaag atctggaatc
361 tcgctattac cgtgatgtga ccgatgcgct aaaaagcgta ccgggtgtga cagtcaccgg
421 agggggcgat actaccgata tcagcattcg tggtatggga tcaaactata ctcttatctt
481 ggtggatggt aagcgccaaa cctcacgcca gacccgtcca aacagcgatg gcccgggcat
541 tgagcaaggt tggttaccgc cactgcaagc gattgaacgt atcgaggtga tccgtggccc
601 gatgtctacg ctgtacggct cggatgcgat tggcggcgtg atcaacatca tcactcgtaa
661 agatcagcag cagtggtcag gcaatgtgca gctatcgacc gtggtacaag aaaatcgcgc
721 ctcaggcgat gaacaaagcg ctaatttctt cgtaacagga cctttaagtg atgctctatc
781 gctgcaagtc tacggacaaa ccacgcaacg cgatgaagac gaaattgagc atggttacgg
841 cgataaatca ctgcgcagct taacctcaaa gctcaactac cagcttaatc cagatcatca
901 actgcaactg gaagccggtg tatcagccca agatcgcgaa aataacgtgg gcaagtcggc
961 gcaatccagc ggatgtcgcg gtacatgcag caataccgat aaccagtacc gtcgcaacca
1021 tgttgccgtc tctcaccaag gggattggca gggtgttggg cagtctgata cttatctgca
1081 atatgaagag aacaccaata aatctcgtga gatgagcatt gataatacgg tgttcaaatc
1141 cacgttagtg gctcctattg gtgagcatat gttgagcttt ggtgtggaag gtaaacacga
1201 aagtttagag gataaaacct ccaacaaaat ttcgtctcgc acgcatatct cgaacactca
1261 atgggcggga ttcattgaag atgaatgggc gctcgcagag caattccgcc tgacctttgg
1321 tggccgtctg gatcatgata aaaactacgg cagccacttc agcccacgtg tgtatggcgt
1381 atggaatctc gatcctctat ggacagtaaa aggtggtgtt tctactggct tccgtgcacc
1441 gcaattacgt gaagtgacgc cggattgggg gcaagttagt ggcggcggta atatttacgg
1501 caacccggat ttacagcctg aaacttcgat taacaaagaa ctgagtttga tgtacagcac
1561 tggctcaggt ctggcggcat cgttaaccgc tttccataat gattttaaag ataaaatcac
1621 ccgcgtcgct tgccctgcaa atatttgtac tgccggcccc aaccaatggg gtgcaactcc
1681 tacttatcgt gtgaatattg atgaagcgga aacctatggt gcagaagcta cgttaagtct
1741 gccgattacc gaaagcgttg agttatcgtc tagctacacg tacacccatt cggagcaaaa
1801 atcagggaat tttgctggcc gtccgctact gcagttacct aaacatctgt tcaatgccaa
1861 cttgagctgg caaaccacag atcgcctcaa tagctgggct aacctcaact atcgtggtaa
1921 ggagatgcag ccggagggtg gggcatcgaa cgatgatttc atcgcgccaa gctacacctt
1981 tatcgatact ggcgtgactt acgcactcac cgataccgca acgattaaag ctgcggtgta
2041 caacctgttt gatcaagagg tgaattacgc ggagtatggc tatgtagaag atggccgccg
2101 ctactggttg ggtttagaca tcgccttcta attgctagag gttcagagga ttggcggcag
2161 cgccaatcct ctg
//