GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS MTXCDHAB 3342 bp ds-DNA BCT 10-MAY-1991
DEFINITION M.soehngenii carbon monoxide dehydrogenase alpha and beta subunit
genes, complete cds.
ACCESSION M55280
KEYWORDS carbon monoxide dehydrogenase.
SOURCE M.soehngenii DNA.
ORGANISM Methanothrix soehngenii
Prokaryota; Bacteria; Mendosicutes; Archaeobacteria;
Methanomicrobiales; Methanosarcinaceae.
REFERENCE 1 (bases 1 to 3342)
AUTHORS Eggen,R.I., Geerling,A.C., Jetten,M.S. and de Vos,W.M.
TITLE Cloning, expression and sequence analysis of the genes for carbon
monoxide dehydrogenase of Methanothrix soehngenii
JOURNAL J. Biol. Chem. 266, 6883-6887 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 2639..3202
/product="carbon monoxide dehydrogenase beta subunit"
/gene="cdhB"
/codon_start=2639
RBS 2625..2635
CDS 238..2619
/product="carbon monoxide dehydrogenase alpha subunit"
/gene="cdhA"
/evidence=EXPERIMENTAL
/codon_start=238
RBS 233..237
misc_feature 202..206
/note="archaeal promoter box B"
misc_feature 171..176
/note="archaeal promoter box A"
BASE COUNT 887 a 842 c 944 g 669 t
ORIGIN
1 aagcttgtgg gcagaggaca cagcattaac aatagtgatc ttactgagtc gctgttaact
61 gaaaatttat ttataaaatt aatactatga tgtttaatca aaattaataa aattctatat
121 taactggtat ccgaaagtgt tttatctact ctatgcgaac tcaagcggcg tatatcaaag
181 gaagaggagg ctaagattcc gatggcaaca agagaaggca gcttttcggt tgaggacatg
241 aagaacgtcc agatcaacat aggagctgtc gtaaaagaag aggatgaatg ggaccaggag
301 atgggcccct tccccaagcc aggtgtggca acgcttcgtg actgggactt caagatttgc
361 aaccggtaca agatcatgta ttccccggca gatgatacat gcaccctctg tacttatggg
421 ccctgcgatc tgaccggcaa caagaagggc gcttgcggca tagacatggc cgctgcctgc
481 ggcaagatcg ttctggtagc ggttttaatg ggaacctgcg cccatactgc tcacggcagg
541 cacctttacc actggtgtct ggacaaattc ggagatatgc ccttcgatat gggcagcgag
601 atcctggtgg atgctcccct ttatcgcacc atcctgggca aaaagcccaa gtcgctcaag
661 gacttcggcg aggccctgga gtattgcgaa gaggagatcg tgcagctcct ggctgcctgt
721 cacaccggtc aggaaggcca ttatatggac ttcgagtcca agtcgctgca cagcggcatg
781 atcgactccc tgggcaagga gatctgcgac atgctgcaga cggtggctta cgatatgccc
841 cgaggcgctg ccgatgcacc cctggtggag atcggcatgg gcaccctgga tcagaataag
901 ggcgttctca tcgcttacgg ccacaacctg gcagcaggcg cagaggccat gatctacacc
961 gaggagcaca atctctggga caaggtcgac atcggcggcg tatgctgtac cgccattgac
1021 ctcaccagga ttactgagac cggcagagaa tccaagatcc ccgccaacct gggaccaaag
1081 gccaaggtgg ctggtgccat gggctggtgg agaaagatgg tccgcgctgg aatcatggat
1141 acagtgattg tggacgagca gtgcgtattc tgcgatgtgc tcgaggactg ccagcaaagg
1201 cacatcccgg ttatcgccag caacgacaag atcatgctcg gcctgcccga caggaccaat
1261 gactcagccg atgccattgt ggaggatctg gtcagcttca agatgcccgg tgtggcaatc
1321 ctcgatccag taaaggccgg agaggtggcc atcaggaccg cggttgcagt caagcccaag
1381 cgcgagcagt acaagaagga gagcctcttt accgagcagc agttcaagga taccctggca
1441 acatgcacag agtgcaatca gtgtgccttc gtctgtccgc cccacatcag gatcagcgag
1501 atgataagcg aggcactgaa gggcaatctg gaaccattct cctctaccta tgaggtatgc
1561 gtgggctgcc agagatgcga gcagacctgc ccccaggaga tccccatcct gaagctctat
1621 gagtacgcca accgcgagta tatcaggaac cagaagttca agatgagagc gggcagggga
1681 cctgtgctcg ataccgagat cagaaaagtg ggcgcacccc tggttcttgg ccagattccg
1741 ggagttatag cgctggtagg atgctcgaat tatcccaacg gcaccaagga gtgctacgac
1801 atcgccaagg agttcgttga cagaggctac atcgtcgttg ccacgggctg catggccatg
1861 gacatgtccc tgtataagga tgaagatggc aagaccatct gggagcagta cgagggtgcc
1921 tttgatggca ggaacatctg caacataggc tcatgtgtgg ccaatgccca cattcatgga
1981 gcagcaatca aggtggcaac catcttcgct cacagaaatg agcgtgccaa ctacgatgat
2041 atcgccgatt atatcatgtc caaggtcgga gcatgcggcg tggcctgggg agcatactcc
2101 caaaaggcag catccatcgc taccggcgtt aacagaatcg gcattccagt ggttgttcag
2161 ccaagctcag tcatataccg caggacattt atgggcagga ccgacaagcc cgaggactgg
2221 atggtcatag atgccaagaa cggcaatatg cagcagatcg agcccgcacc tgaagccatg
2281 ctctatatcg ccgagaccaa ggaggaggcc atgctggaga tggcaaagct ctgcttccgc
2341 ccgtctgata acacccaggg cagaggaatc aagctgaccc attactgcga tatctccatg
2401 aagtacttcg gcaagctgcc cgatgactgg catctctttg tccgcgatgt caaggatctg
2461 cccctgaact accagacgca gatgatgaag gagctggagg agaagcacgg ctggaagatc
2521 gactggaagg cgaagaagtt catctccgga ccgctcaggc ccgcagacgt cagcttcgat
2581 cccacgaaca tcccgcgcaa gataagggcc aagaagtgag gacgaggagg agatagaaat
2641 ggcagcagaa gttaagaagg gcattgacac caccaagaat cccattccct ttgagatggc
2701 tcagatccct gggccagaga tggcgaagac ctatttgccg aaggtaatcg gcgcgatcat
2761 cagaaaggcg aagaggcctc tgctcgtggt aggagcagag ctcttcgacg atcccgtgat
2821 gttcgataag atgattgaga tgggaaagat gggaattccc atcgccgcca cggcgcatag
2881 cgtcaagggc tttgtggata gaggctacct ggagaacgtc tatcagatcg gcctgcatcc
2941 gttgaccaac tttttgaggt tccccgactg gaagggcctg gatggacagg gacagtacga
3001 tgtggtcata ttcctgggca tctactacaa gttcgccaat ggcatgctat ccacattgaa
3061 gaacttcaac cgggacatca agcgcgtctc catagaccgc tactatcatg tcaatgccga
3121 tatgaccttc ggcaacctgg ccttcaatcc ggacgactat cacgcagccg tagacgaagt
3181 aatcgcagcg atgaagaaat agagtttatc ctataacaga agaaaacaga agaggtgtat
3241 taatggcaga tataaagttg gacatatccc ccatgtatga gggcgagaga atcagaaggg
3301 acgatctatg ggtagagctt ggcggtccca agcagatgat tt
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