GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS SYNMOV1 6366 bp ds-DNA SYN 10-MAY-1991
DEFINITION Moloney murine leukemia virus retroviral vector pLXSHD, complete
sequence.
ACCESSION M64753
KEYWORDS cloning vector; histidinol dehydrogenase; retroviral vector.
SOURCE Synthetic, DNA.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 6366)
AUTHORS Stockshlaeder,M.A., Storb,R., Osborne,W.R. and Miller,A.D.
TITLE L-histidinol provides effective selection of
retrovirus-vector-transduced keratinocytes without impairing their
proliferative potential
JOURNAL Hum. Gene Ther. 2, 33-39 (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
misc_recomb 174^175
/note="mouse DNA end/Mo-MuSV DNA start"
LTR 175..763
/note="Mo-MuSV 5' long terminal repeat"
misc_signal 833..1634
/note="extended packaging signal"
misc_recomb 1159^1160
/note="Mo-MuSV DNA end/Mo-MuLV DNA start"
mutation 1215..1217
/note="gag ATG start codon to TAG stop codon"
misc_recomb 1646^1647
/note="Mo-MuLV DNA end/simian virus 40 DNA start"
promoter 1647..1986
/note="simian virus 40 early promoter"
misc_recomb 1986^1987
/note="simian virus 40 DNA end/hisD DNA start"
CDS 1996..3300
/EC_number="1.1.1.23"
/product="histidinol dehydrogenase"
/codon_start=1996
misc_recomb 3340^3341
/note="hisD DNA end/Mo-MuLV DNA start"
LTR 3412..4005
/note="Mo-MuLV 3' long terminal repeat"
misc_recomb 4074^4075
/note="Mo-MuLV DNA end/plasmid pBR322 DNA start"
BASE COUNT 1480 a 1777 c 1626 g 1483 t
ORIGIN
1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
241 atacataact gagaatagga aagttcagat caaggtcagg aacaaagaaa cagctgaata
301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
1141 ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt
1201 atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc ttaggtcact
1261 ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg
1321 ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct
1381 ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac
1441 acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct
1501 gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc
1561 tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc
1621 cttctctagg cgccggaatt cgttaactcg aggatccggc tgtggaatgt gtgtcagtta
1681 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
1741 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
1801 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
1861 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
1921 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
1981 ggcagatccc ggaccatgag cttcaatacc ctgattgact ggaacagctg tagccctgaa
2041 cagcagcgtg cgctgctgac gcgtccggcg atttccgcct ctgacagtat tacccggacg
2101 gtcagcgata ttctggataa tgtaaaaacg cgcggtgacg atgccctgcg tgaatacagc
2161 gctaaatttg ataaaacaga agtgacagcg ctacgcgtca cccctgaaga gatcgccgcc
2221 gccggcgcgc gtctgagcga cgaattaaaa caggcgatga ccgctgccgt caaaaatatt
2281 gaaacgttcc attccgcgca gacgctaccg cctgtagatg tggaaaccca gccaggcgtg
2341 cgttgccagc aggttacgcg tcccgtctcg tctgtcggtc tgtatattcc cggcggctcg
2401 gctccgctct tctcaacggt gctgatgctg gcgacgccgg cgcgcattgc gggatgccag
2461 aaggtggttc tgtgctcgcc gccgcccatc gctgatgaaa tcctctatgc ggcgcaactg
2521 tgtggcgtgc aggaaatctt taacgtcggc ggcgcgcagg cgattgccgc tctggccttc
2581 ggcagcgagt ccgtaccgaa agtggataaa atttttggcc ccggcaacgc ctttgtaacc
2641 gaagccaaac gtcaggtcag ccagcgtctc gacggcgcgg ctatcgatat gccagccggg
2701 ccgtctgaag tactggtgat cgcagacagc ggcgcaacac cggatttcgt cgcttctgac
2761 ctgctctccc aggctgagca cggcccggat tcccaggtga tcctgctgac gcctgatgct
2821 gacattgccc gcaaggtggc ggaggcggta gaacgtcaac tggcggaact gccgcgcgcg
2881 gacaccgccc ggcaggccct gagcgccagt cgtctgattg tgaccaaaga tttagcgcag
2941 tgcgtcgcca tctctaatca gtatgggccg gaacacttaa tcatccagac gcgcaatgcg
3001 cgcgatttgg tggatgcgat taccagcgca ggctcggtat ttctcggcga ctggtcgccg
3061 gaatccgccg gtgattacgc ttccggaacc aaccatgttt taccgaccta tggctatact
3121 gctacctgtt ccagccttgg gttagcggat ttccagaaac ggatgaccgt tcaggaactg
3181 tcgaaagcgg gcttttccgc tctggcatca accattgaaa cattggcggc ggcagaacgt
3241 ctgaccgccc ataaaaatgc cgtgaccctg cgcgtaaacg ccctcaagga gcaagcatga
3301 gcactgaaaa cactctcagc gtcgctgact tagcccggga tctgcctagg cttttgcaaa
3361 aagcttatcg ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
3421 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa
3481 tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata
3541 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
3601 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
3661 agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca
3721 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
3781 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
3841 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt
3901 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
3961 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg
4021 gatcgggaga cccctgccca gggaccaccg acccaccacc gggaggtaag ctggctgcct
4081 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
4141 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
4201 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
4261 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
4321 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
4381 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
4441 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
4501 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
4561 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
4621 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
4681 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
4741 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
4801 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
4861 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
4921 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
4981 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
5041 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
5101 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
5161 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
5221 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
5281 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
5341 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
5401 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
5461 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
5521 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
5581 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
5641 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
5701 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
5761 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
5821 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
5881 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
5941 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
6001 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
6061 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
6121 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
6181 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
6241 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
6301 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
6361 cttcaa
//